Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli

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• 作者：Xiangwei Wang^a ; ^b ; Lingling Li^a ; ^c ; Yanning Zheng^a ; ^b ; Huibin Zou^a ; Yujin Cao^a ; Hui Liu^a ; Wei Liu^a ; Mo Xian^a ; ^{xianmo@qibebt.ac.cn}
• 关键词：Long chain hydroxyfatty acid ; Escherichia coli ; Thioesterase ; Fatty acid hydroxylase ; fadD knocking out
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：561-566
• 文件大小：350 K

摘要

This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 鈥楾esA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450_BM3 to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0 mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources.