Preparation of thiolated polymeric nanocomposite for sensitive electroanalysis of dopamine
- 作者：Zhaohong Su ; Ying Liu ; Qingji Xie<sup> ; sup><sup>xieqj@hunnu.edu.cnsup> ; Li Chen ; Yi Zhang ; Yue Meng ; Yan Li ; Yingchun Fu ; Ming Ma ; Shouzhuo Yao
- 关键词：Thiol-ene chemistry ; Polypyrrole ; Carboxylated thiols ; Multiwalled carbon nanotubes ; Dopamine electroanalysis
- 刊名：Biosensors and Bioelectronics
- 出版年：2012
- 期刊代码：12_09565663
- 类别：ch
- 出版时间：June-July, 2012
- 卷：36
- 期：1
- 页码：154-160
- 文件大小：722 K
摘要
We report on the thiol-ene chemistry guided preparation of novel thiolated polymeric nanocomposite films of abundant anionic carboxylic groups for electrostatic enrichment and sensitive electroanalysis of cationic dopamine (DA) in neutral solution. Briefly, the thiol-ene nucleophilic reaction of a carboxylated thiol with oxidized polypyrrole (PPy), which was electrosynthesized on an Au electrode in the presence of solution-dispersed acidified multiwalled carbon nanotubes (MWCNTs), produced an a PPy-thiol-MWCNTs/Au electrode, and the PPy can be electrochemically overoxidized (OPPy) to form an OPPy-thiol-MWCNTs/Au electrode. The carboxylic groups of the polymeric nanocomposite film originate from the acidified MWCNTs, PPy-tethered carboxylated thiol, and OPPy. The carboxylated thiols examined are mercaptosuccinic acid (MSA) and thioglycolic acid, with 尾-mercaptoethanol as a control. Electrochemical quartz crystal microbalance, scanning electron microscopy, Fourier transform infrared spectroscopy and ultraviolet-visible spectroscopy were used for film characterization and process monitoring. Under the optimized condition, the differential pulse voltammetry peak current of DA oxidation at OPPy-MSA-MWCNTs/Au electrode is linear with DA concentration from 1.00脳10<sup>-9sup> to 2.87脳10<sup>-6sup> mol L<sup>鈭?sup>, with a limit of detection of 0.4 nmol L<sup>鈭?sup>, good anti-interferent ability and stability.