miR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3

详细信息	查看全文 | 推荐本文 |

• 作者：Zhengrong Li^a ; ¹ ; ^{lizhengrong08@yahoo.com.cn} ; Yi Cao^a ; ¹ ; Zhigang Jie^a ; ^{jiezg123@126.com} ; Yi Liu^a ; Yingliang Li^a ; Junhe Li^b ; Guoming Zhu^a ; Zhengren Liu^a ; Yi Tu^c ; Gen Peng^a ; Dong-woo Lee^d ; Sung-soo Park^d
• 关键词：miR-495 ; miR-551a ; PRL-3 ; Gastric carcinoma
• 刊名：Cancer Letters
• 出版年：2012
• 期刊代码：44_03043835
• 类别：med
• 出版时间：1 October, 2012
• 卷：323
• 期：1
• 页码：41-47
• 文件大小：1182 K

摘要

The phosphatase of regenerating liver-3 (PRL-3) gene is associated with metastasis in gastric cancer, and is believed to play a causative role by promoting tumor cell motility, invasion, and metastasis, but little is known of the mechanisms involved. We previously reported that PRL-3 expression is significantly higher in the tissues of primary gastric carcinomas with peritoneal metastasis. In the present study, we found that two microRNAs (miRNAs), miR-495 and miR-551a, predicted to target PRL-3, are downregulated in gastric carcinoma samples. The validation of this interaction between those two miRNAs and PRL-3 was confirmed by western blotting and quantitative real-time PCR (qPCR) in GC cell lines transfected with miR-495 and miR-551a mimics. Furthermore, the migration and invasion of GC cells were significantly inhibited by transfection with miR-495 or -551a mimics, and the mRNA and protein levels of PRL-3 were reduced in cells overexpressing miR-495 or -551a. Collectively, our findings suggest that miR-495 and miR-551a both act as tumor suppressors by targeting the PRL-3 oncogene and inhibiting gastric cancer cell migration and invasion. The findings of this study contribute to current understanding of the functions of miRNA mimics in GC gene therapy.