摘要
采用序批式生物膜反应器(SBBR),以混合污泥作为接种污泥,研究了反应器启动过程中菌群演替规律。结果表明,通过逐步提高进水NH4+-N和NO2--N含量的方式将SBBR的N容积负荷由0.10 g/(L·d)提升至1.164 g/(L·d),经过144 d成功启动厌氧氨氧化SBBR,TN去除率达93.92%,厌氧氨氧化活性为5.86 mg/(g·h)。在反应器启动过程中,厌氧氨氧化菌的丰度不断升高,其它非功能菌的丰度逐渐降低。稳定运行期,浮霉菌门在活性污泥和生物膜上的丰度有显著的差异,前者为8.83%,后者为24.21%。3种检出的厌氧氨氧化菌属在活性污泥和生物膜上的差异也十分明显,Candidatus Jettenia、Candidatus Brocadia和Candidatus Kuenenia在活性污泥中的丰度分别为2.89%、2.29%和0.65%,而在生物膜的丰度分别为10.04%、5.93%、2.22%。
The SBBR reactor was used to inoculate the sludge with the mixed sludge, and the succession of the bacterial community during the start-up of the reactor was studied. The results showed the N volumetric load of the SBBR reactor was increased from 0.10 g/(L·d) to 1.164 g/(L·d) by gradually increasing the content of influent NH4+-N and NO2--N. After 144 d, the anammox SBBR was successfully started, and the TN removal rate was 93.92%and an anammox activity was 5.86 mg/(g·h). That the abundance of anammox bacteria increased continuously during the start-up of the reactor, and the abundance of other non-functional bacteria gradually decreased. During the stable operation period, there was a significant difference in the abundance of the Planctomycetes on the activated sludge and biofilm, the former being 8.83% and the latter being 24.21%. The differences between the three detected anammox species in activated sludge and biofilm were also obvious. The abundances of Candidatus Jettenia, Candidatus Brocadia and Candidatus Kuenenia in activated sludge were 2.89%, 2.29% and 0.65%, respectively, while the abundance of biofilms was 10.04%, 5.93%, and 2.22%, respectively.
引文
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