灰茶尺蠖雌成虫生殖器形态特征与组织结构的研究
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摘要
为了解茶树主要食叶类害虫灰茶尺蠖(EctropisgrisescensWarren)雌成虫的内外生殖器结构特征,通过解剖切片和显微观察的方法,明确了雌成虫内外生殖器主要由1对卵巢、1对侧输卵管、1根中输卵管、1个受精囊、附腺、产卵孔、交配囊及其附属结构、导精管、表皮突和肛突等组成。卵巢小管8根,左右对称;交配囊呈典型袋状结构,内有表面布满刺状突的心型交尾囊片,且囊片左右两侧16根刺状突呈对称分布;后表皮突较细长,似铲状。切片观察可知,卵巢管滋养细胞和卵母细胞交替排列,为典型的多滋式;产卵器内有发达的性腺等。掌握灰茶尺蠖雌成虫生殖器结构,对其卵巢发育级别划分、田间预测预报、近缘种的鉴定以及性信息素的研究等具有重要意义。
The tea geometrid, Ectropis grisescens Warren is a major defoliator of tea trees. The morphology and histology of internal and external reproductive organs of the female adult insects were described here using both anatomical sections and microscopic observation. The female adult reproductive organs were comprised of a pair of ovaries, two lateral oviducts, one common oviduct, spermatheca, accessory gland, oviporus, corpus bursae and associated parts, ductus seminalis, apophyses and papillae anales. Each ovary contained eight symmetrical ovarioles. The corpus bursae was a typical bag structure and possessed a heart-shaped signum which covered 16 symmetrical spines on the surface of both sides. The apophyses posteriores was slender and shovel-like. Nurse cells and oocytes were arranged alternately in polytrophic ovarioles. The ovipositor possessed a well-developed gonad. This study was the first research on the genital structure of female adult of E. grisescens Warren. The results provided guidance for the study of ovarian development, field forecasting, and identification of related species.
The tea geometrid, Ectropis grisescens Warren is a major defoliator of tea trees. The morphology and histology of internal and external reproductive organs of the female adult insects were described here using both anatomical sections and microscopic observation. The female adult reproductive organs were comprised of a pair of ovaries, two lateral oviducts, one common oviduct, spermatheca, accessory gland, oviporus, corpus bursae and associated parts, ductus seminalis, apophyses and papillae anales. Each ovary contained eight symmetrical ovarioles. The corpus bursae was a typical bag structure and possessed a heart-shaped signum which covered 16 symmetrical spines on the surface of both sides. The apophyses posteriores was slender and shovel-like. Nurse cells and oocytes were arranged alternately in polytrophic ovarioles. The ovipositor possessed a well-developed gonad. This study was the first research on the genital structure of female adult of E. grisescens Warren. The results provided guidance for the study of ovarian development, field forecasting, and identification of related species.
引文
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[2]Kim S S,Beljaev E A,Oh S H.I llustrated catalogue of Geometridae in Korea(Lepidoptera:Geometrinae,Ennominae)[M]//Park KT.Insects of Korea Series 8.Korea:Korea Research Institute of Bioscience and Biotechnology&Center for Insect Systematics,2001.
[3]Prout L B.On the Japanese Geometridae of the Aigner collection[J].Novitates Zoologicae,1930,35:289-377.
[4]葛超美.灰茶尺蠖的生物学特性及其体色遗传规律研究[D].北京:中国农业科学院,2016.
[5]Sziráki G.Contribution to knowledge of female internal genitalia of Neuroptera[J].Acta Zoologica Academiae Scientiarum Hungarica,2002,48(s2):341-349.
[6]王伟芹,马丽丽,Sziráki G,等.中国粉蛉属四种雌虫的分类学研究(脉翅目:粉蛉科)[J].昆虫分类学报,2007,29(1):19-25.
[7]王宪辉,徐洪富,徐永玉,等.甜菜夜蛾雌性生殖系统结构、发育分级及在测报上的应用[J].植物保护学报,2003,30(3):261-266.
[8]林玉英,金涛,甘挺峰,等.椰子木蛾生殖系统初步研究[J].热带作物学报,2017,38(9):1704-1711.
[9]常明山,吴耀军,刘文爱,等.桐花树毛颚小卷蛾雌成虫内生殖系统研究[J].中国森林病虫,2013,32(2):1-6.
[10]黄忠宝.条沙叶蝉Psammotettix striatus(L.)生殖系统的形态和超微结构研究[D].杨凌:西北农林科技大学,2011.
[11]刘颖,徐鹏辉,陈舟,等.柑橘大实蝇雌成虫卵巢发育的分级[J].华中农业大学学报,2015,34(1):33-37.
[12]张家侠,孙钦玉,赵强,等.茶尺蠖雄成虫生殖系统形态学与组织学观察[J].茶叶科学,2015,35(6):527-533.
[13]王晓芳.中国黄粉蝶亚科昆虫雌性生殖系统研究(鳞翅目:粉蝶科)[D].杨凌:西北农林科技大学,2010.
[14]孟银凤,尚素琴,张雅林.橙黄豆粉蝶生殖系统形态学研究[J].应用昆虫学报,2013,50(3):813-817.
[15]Ma M,Chang MM,Lu Y,et al.Ultrastructure of sensilla of antennae and ovipositor of Sitotroga cerealella(Lepidoptera:Gelechiidae),and location of female sex pheromone gland[J].Scientific Reports,2017,7:40637.
[16]曹帅,尚素琴,张雅林.黑纹粉蝶Pieris melete Ménétriès生殖系统解剖学研究(鳞翅目:粉蝶科)[J].西北农林科技大学学报(自然科学版),2012,40(9):77-82.
[17]王丽平,嵇保中,刘曙雯,等.微红梢斑螟生殖系统及卵巢发育特征研究[J].应用昆虫学报,2015,52(4):844-853.
[18]李卫春.中国苔螟亚科和草螟亚科系统学研究(鳞翅目:螟蛾总科:草螟科)[D].天津:南开大学,2010.
[19]王颖.东北地区尺蛾亚科分类研究(鳞翅目:尺蛾科)[D].哈尔滨:东北林业大学,2010.
[20]刘淑蓉.中国祝蛾亚科分类学研究(鳞翅目:祝蛾科)[D].天津:南开大学,2014.
[21]丁昌萍.中国粉蝶族昆虫系统分类研究(鳞翅目:粉蝶科:粉蝶亚科)[D].杨凌:西北农林科技大学,2011.
[22]刘晓燕,吴孝兵.中国黄粉蝶亚科六属间基于COⅡ和EF-1α基因部分序列的系统发育关系[J].昆虫学报,2007,50(6):604-609.
[23]Braby M F,Vila R,Pierce N E.Molecular phylogeny and systematics of the Pieridae(Lepidoptera:Papilionoidea):higher classification and biogeography[J].Zoological Journal of the Linnean Society,2006,147(2):239-275.