基于油菜籽碳量子点荧光恢复测定盐酸米诺环素的研究
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摘要
以油菜籽和水为原料,一步合成了强荧光碳量子点(CQDs)。基于KMnO_4能使该CQDs的荧光显著猝灭;而当有盐酸米诺环素(MINO)存在时,CQDs-KMnO_4体系的荧光恢复,使荧光信号重新"打开"的原理,提出了以油菜籽CQDs荧光信号的"关开"为荧光探针测定盐酸米诺环素的新方法。方法的线性范围为5.0×10~(-7)～1.0×10~(-5) mol/L,相关系数r=0.999 5,方法检出限为4.9×10~(-7) mol/L。方法用于样品中盐酸米诺环素的测定,回收率为99.6%～100%。通过紫外-可见吸收光谱的变化和荧光寿命的测定,确定CQDs与KMnO_4间的猝灭类型为动态猝灭。同时对体系的"关-开"机理进行了初步探讨。
In this paper,a new fluorescent carbon quantum dots(CQDs)were synthesized by one-step method using rapeseed and water as raw material.The experiments showed that potassium permanganate(KMnO_4)could effectively quench the fluorescence of the CQDs.However,the fluorescence of CQDs-KMnO_4 system restored reversibly in the presence of minocycline hydrochloride.Based on this principle,a new method was proposed for the determination of minocycline hydrochloride with the"off-on"fluorescence signal of rapeseed CQDs as a fluorescent probe.Under the optimum conditions,the linear range of the method was 5.0×10~(-7) mol/L to 1.0×10~(-5) mol/L with a correlation coefficient of 0.999 5 and a detection limit of 4.9×10~(-7) mol/L.The method was used in the determination of minocycline hydrochloride in samples with a recovery of 99.6%-100%.The mechanisms for CQDs fluorescence quenched by KMnO_4 and recovered by minocycline hydrochloride were studied.
In this paper,a new fluorescent carbon quantum dots(CQDs)were synthesized by one-step method using rapeseed and water as raw material.The experiments showed that potassium permanganate(KMnO_4)could effectively quench the fluorescence of the CQDs.However,the fluorescence of CQDs-KMnO_4 system restored reversibly in the presence of minocycline hydrochloride.Based on this principle,a new method was proposed for the determination of minocycline hydrochloride with the"off-on"fluorescence signal of rapeseed CQDs as a fluorescent probe.Under the optimum conditions,the linear range of the method was 5.0×10~(-7) mol/L to 1.0×10~(-5) mol/L with a correlation coefficient of 0.999 5 and a detection limit of 4.9×10~(-7) mol/L.The method was used in the determination of minocycline hydrochloride in samples with a recovery of 99.6%-100%.The mechanisms for CQDs fluorescence quenched by KMnO_4 and recovered by minocycline hydrochloride were studied.
引文
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[6] Zhang H J,Shi J,Jin X Y,Peng J.J.Instrum.Anal.(张慧佳,石静,晋晓勇,彭娟.分析测试学报),2018,37(6):724-728.
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[11] Hoshino M,MorI I,Motomatsu M,Nakatani K,Yamaguchi T,Fujita Y.Bunseki Kagaku,2001,50(8):547-551.
[12] Huang H,Xu Y,Tang C J,Chen J R,Wang A J,Feng J J.New J.Chem.,2014,38(2):784-789.
[13] Liu J,Liu X L,Luo H J,Gao Y F.RSC Adv.,2014,4:7648-7654.
[14] Hou C Y,Li H Y.China Pharm.(侯昌元,李红玉.中国药师),2011,14(6):821-823.
[15] Tang L B,Ji R B,Li X M,Teng K S,Shu P L.Part.Part.Syst.Charact.,2013,30(6):523-531.
[16] Wang J Y,Zhang Y C,Ma H Y,Liu X L,Wang Q.Chin.J.Lumin.(王靖原,张越诚,马红燕,刘晓莉,王琦.发光学报),2018,39(4):600-607.
[17] Huang S,Zhu F W,Xiao Q,Su W,Seng J R,Huang C S,Hu B Q.RSC Adv.,2014,4:46751-46761.