通过CHD基因鉴定信鸽性别
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摘要
本试验采用羽毛样本成功提取了信鸽基因组DNA,然后利用引物对2550F/2718R对其CHD基因的特异性片段进行扩增,琼脂糖凝胶电泳检测结果显示,雌性信鸽有2条带,分别在400 bp与600 bp左右,雄性仅有1条带,在400 bp左右。而后利用此方法,对600只性别未知的信鸽进行了性别鉴定,其中雄性信鸽358只,雌性信鸽242只,准确率达到100%,结果表明:本试验的分子生物学方法可用于鉴别信鸽性别,提高养殖经济效益,在生产管理上有广阔的应用前景。
The primer pairs(2550F/2718R) was desgned and polymerase chain reaction(PCR) technique to amplificate the segments of CHD gene to identificate pigeon's sex using a DNA sample which was extracted from feather pulps.Results showed that a DNA fragment(about 400 bp) was amplified from male pigeons, and two DNA fragments(about 400 bp and 600 bp) were amplified from the female. Then 600 unknown sex samples were successfully identified by this PCR technique(358 females and 242 males). This technique is useful in the economic and the management of pigeon production.
The primer pairs(2550F/2718R) was desgned and polymerase chain reaction(PCR) technique to amplificate the segments of CHD gene to identificate pigeon's sex using a DNA sample which was extracted from feather pulps.Results showed that a DNA fragment(about 400 bp) was amplified from male pigeons, and two DNA fragments(about 400 bp and 600 bp) were amplified from the female. Then 600 unknown sex samples were successfully identified by this PCR technique(358 females and 242 males). This technique is useful in the economic and the management of pigeon production.
引文
[1]Griffiths R,Tiwari B.Sex of the last wild Spix's macaw[J].Nature,1995,375(6531):454.
[2]Fridolfsson A K,Ellegren H.A simple and universal method for molecular sexing of non-ratite birds[J].J Avian Biol,1999,116-121.
[3]Cerit H,Avanus K.鸟类性别鉴别方法的研究进展[J].中国家禽,2008,30(18):30-33.
[4]Horváth M B,Martínez-Cruz B,Negro J J,et al.An overlooked DNA source for non-invasive genetic analysis in birds[J].J Avian Biol,2005,36(1):84-88.
[5]Leeton P.Feathers from museum bird skins-a good source of DNA for phylogenetic studies[J].Condor,1993,95:465-466.
[6]Bello N,Francino O,Sánchez A.Isolation of genomic DNA from feathers[J].J Vet Diagnos Investigat,2001,13(2):162-164.
[7]胡飞,张红霞,包文斌,等.黑冠鹤性别的分子鉴定方法[J].中国畜牧兽医,2008,34(11):61-63.
[8]Alipanah M.Sex determination of Ostrich(struthio camelus)using DNA markers[J].Can J Anim SCi,2010,90(3):357-360.
[9]盖玉林,路迪,周材权.两种观赏鸟性别的快速鉴定研究[J].西华师范大学学报(自然科学版),2014,35(2):166-171.
[10]Griffiths R,Daan S,Dijkstra C.Sex identitication in biirds using two CHD genes[J].Proc Biol Sci,1996,263(1374):1251-1256.
[11]Griffiths R,Double M C,Orr K,et al.A DNA test to sex most birds[J].Mol Ecol 1998,7(8):1071-1075.
[12]Sacchi P,Soglia D,Maione S,et al.A non-invasive test for sex identification in Short-toed Eagle(Circaetus gallicus)[J].Mol Cell Probes,2004,18(3):193-196.
[13]Cerit H,Avanus K.Sex determination by CHDW and CHDZ genes of avian sex chromosomes in Nymphicus hollandicus[J].Turkish J Veterinary Anim Sci,2007,31(6):100-103.
[14]陈宇科,林海,赵苗苗,等.PCR法鉴定雏鸽性别的初步研究[J].经济动物学报,2013,17(4):203-206.
[15]陈蓉,陈楠,章小小,等.美洲红鹮和火烈鸟性别的分子鉴定[J].畜牧与兽医,2014,36(1):60-62.
[16]田秀华,刘铸,阿相宝,等.7种鹤形目鸟类性别的分子鉴定[J].动物学杂志,2006,41(5):62-67.
[17]刘铸,田秀华,白素英.一种准确简便的东方白鹤性别分子鉴定方法[J].野生动物,2006,27(3):50-53.
[2]Fridolfsson A K,Ellegren H.A simple and universal method for molecular sexing of non-ratite birds[J].J Avian Biol,1999,116-121.
[3]Cerit H,Avanus K.鸟类性别鉴别方法的研究进展[J].中国家禽,2008,30(18):30-33.
[4]Horváth M B,Martínez-Cruz B,Negro J J,et al.An overlooked DNA source for non-invasive genetic analysis in birds[J].J Avian Biol,2005,36(1):84-88.
[5]Leeton P.Feathers from museum bird skins-a good source of DNA for phylogenetic studies[J].Condor,1993,95:465-466.
[6]Bello N,Francino O,Sánchez A.Isolation of genomic DNA from feathers[J].J Vet Diagnos Investigat,2001,13(2):162-164.
[7]胡飞,张红霞,包文斌,等.黑冠鹤性别的分子鉴定方法[J].中国畜牧兽医,2008,34(11):61-63.
[8]Alipanah M.Sex determination of Ostrich(struthio camelus)using DNA markers[J].Can J Anim SCi,2010,90(3):357-360.
[9]盖玉林,路迪,周材权.两种观赏鸟性别的快速鉴定研究[J].西华师范大学学报(自然科学版),2014,35(2):166-171.
[10]Griffiths R,Daan S,Dijkstra C.Sex identitication in biirds using two CHD genes[J].Proc Biol Sci,1996,263(1374):1251-1256.
[11]Griffiths R,Double M C,Orr K,et al.A DNA test to sex most birds[J].Mol Ecol 1998,7(8):1071-1075.
[12]Sacchi P,Soglia D,Maione S,et al.A non-invasive test for sex identification in Short-toed Eagle(Circaetus gallicus)[J].Mol Cell Probes,2004,18(3):193-196.
[13]Cerit H,Avanus K.Sex determination by CHDW and CHDZ genes of avian sex chromosomes in Nymphicus hollandicus[J].Turkish J Veterinary Anim Sci,2007,31(6):100-103.
[14]陈宇科,林海,赵苗苗,等.PCR法鉴定雏鸽性别的初步研究[J].经济动物学报,2013,17(4):203-206.
[15]陈蓉,陈楠,章小小,等.美洲红鹮和火烈鸟性别的分子鉴定[J].畜牧与兽医,2014,36(1):60-62.
[16]田秀华,刘铸,阿相宝,等.7种鹤形目鸟类性别的分子鉴定[J].动物学杂志,2006,41(5):62-67.
[17]刘铸,田秀华,白素英.一种准确简便的东方白鹤性别分子鉴定方法[J].野生动物,2006,27(3):50-53.