金属离子对中等嗜热混合菌活性的影响
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摘要
通过金属耐受试验,测定了金属离子Mo~(6+)、Cu~(2+)、Zn~(2+)对中等嗜热混合菌(喜温嗜酸硫杆菌和氧化亚铁微螺菌共培养体系)活性的影响。结果表明,随着金属离子浓度的提高,细菌的活性降低,受试菌对上述3种金属离子的耐受浓度分别为0.6g/L、10 g/L和30 g/L,3种金属离子的毒性顺序为:Mo~(6+)>Cu~(2+)>Zn~(2+)。喜温嗜酸硫杆菌对金属离子的耐受能力高于氧化亚铁微螺菌。结果说明喜温嗜酸硫杆菌和氧化亚铁微螺菌共培养体系具有较强的金属离子耐受能力,特别是耐受钼的能力很强,适于含钼硫化矿、含钼废料以及含钼污泥等的生物浸出。
The effects of metal ions Mo~(6+),Cu~(2+),and Zn~(2+) on the activities of mixed moderately thermophilic bacteria such asLeptospirillum ferriphilum and Acidithiobacillus caldus,were tested by the metal tolerance experiment. As results,the activities of mixmoderately thermophilic bacteria decreased with increasing concentration of metal ions. The tolerated concentration of bacteria tested for metalions Mo~(6+),Cu~(2+) and Zn~(2+) were 0.6 g/L,10 g/L and 30 g/L respectively. The toxicity order of metal ions was in Mo~(6+) >Cu~(2+)>Zn~(2+). A. calduspresented a higher degree of resistance to metal ions than L. ferriphilum. Results in this study demonstrated that L. ferriphilum and A. caldusin co-culture had strong resistance to metal ions,especially to Mo~(6+),thus it can be applied to the bioleaching for molybdenum sulphide ore,molybdenum waste and molybdenum sludge.
The effects of metal ions Mo~(6+),Cu~(2+),and Zn~(2+) on the activities of mixed moderately thermophilic bacteria such asLeptospirillum ferriphilum and Acidithiobacillus caldus,were tested by the metal tolerance experiment. As results,the activities of mixmoderately thermophilic bacteria decreased with increasing concentration of metal ions. The tolerated concentration of bacteria tested for metalions Mo~(6+),Cu~(2+) and Zn~(2+) were 0.6 g/L,10 g/L and 30 g/L respectively. The toxicity order of metal ions was in Mo~(6+) >Cu~(2+)>Zn~(2+). A. calduspresented a higher degree of resistance to metal ions than L. ferriphilum. Results in this study demonstrated that L. ferriphilum and A. caldusin co-culture had strong resistance to metal ions,especially to Mo~(6+),thus it can be applied to the bioleaching for molybdenum sulphide ore,molybdenum waste and molybdenum sludge.
引文
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[2]贾红秀,高丽梅,姜威.钼市场30年回顾与展望[J].中国钼业,2006,30(1):42-47.
[3]吕发奎.辉钼矿与难选钼矿的柱式高效分选工艺研究[D].徐州:中国矿业大学,2010:22.
[4]杨显万,沈庆峰,郭玉霞.微生物湿法冶金[M].北京:冶金工业出版社,2003:123-125.
[5]Dew DW,Buuren CV,Mcewan K,et al.Bioleaching of base metalsulphide concentrates:A comparison of mesophile and thermophilebacterial cultures[J].Process Metallurgy,1999,9(99):229-238.
[6]Klaus B.Bioleaching:Metal solubilization by microorganisms[J].FEMS Microbiology Reviews,1997,20:591-604.
[7]石绍渊,方兆衍.氧化亚铁硫杆菌与中度嗜热铁氧化菌浸出铁闪锌矿的比较[J].过程工程学报,2005,5(4):384-388.
[8]谢海云,陆建生,蓝卓越,等.中等嗜热菌浸出高砷铜精矿研究[J].昆明理工大学学报:理工版,2010,01:24-28.
[9]王有团,李广悦,刘玉龙,等.JX嗜酸异养菌与氧化亚铁硫杆菌联合浸铀的研究[J].有色金属(冶炼部分),2010(2):42-45.
[10]吉兆宁,余斌,刘坚,等.金堆城低品位钼矿石可浸性研究[J].有色金属(矿山部分),2002,54(5):15-18,26.
[11]Pradhan D,Ahn JG,Kim DJ,et al.Effect of Ni2+,V4+andMo6+concentration on iron oxidation by Acidithiobacillus ferrooxidans[J]Korean Journal of Chemical Engineering,2009,26(3):736-741.
[12]Zamani MAA,Hiroyoshi N,Tsunekawa M,et al.Bioleachingof sarcheshmeh molybdenum concentrate for extraction ofrhenium[J].Hydrometallurgy,2005,80:23-31.
[13]Olson GJ,Clark TR.Bioleaching of molybdenite[J].Hydrometallurgy,2008,93:10-15.