中药雄黄的微生物炮制条件优化
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摘要
利用铁氧化菌优选中药雄黄微生物炮制方法的最佳工艺条件。采用毛细管电泳法测定雄黄中可溶性砷盐的含量,分别考察初始pH,炮制时间,雄黄粒径,雄黄矿浆浓度以及Fe2+浓度对雄黄微生物炮制的影响。利用铁氧化菌优选的雄黄微生物炮制最优工艺条件为:初始pH 2.5、炮制时间27 d、雄黄粒径75μm、矿浆浓度2.5%(w/v),Fe2+离子浓度1 g·L-1。初步确定了铁氧化菌生物炮制雄黄的有利条件,为雄黄炮制品的进一步开发提供了一定的科学依据。
To optimize the microbial processing condition of Chinese medicine Realgar by iron-oxidizing bacteria,the content of dissolved arsenic of Realgar was established by capillary,the factors were researched including initial pH,bioleaching time,the particle size of Realgar,the slurry concentration of Realgar and Fe2+ ion concentration,separately. The optimized microbial processing condition by iron-oxidizing bacteria was pH2.5,bioleaching time of 27 days,the particle size 75 μm of Realgar,the slurry concentration of 2.5 %(w/v)and Fe2+ ion concentration of 1 g·L-1. The optimized microbial processing condition was estabilished by iron-oxidizing bacteria,which could be used to research the processed products of Realgar.
To optimize the microbial processing condition of Chinese medicine Realgar by iron-oxidizing bacteria,the content of dissolved arsenic of Realgar was established by capillary,the factors were researched including initial pH,bioleaching time,the particle size of Realgar,the slurry concentration of Realgar and Fe2+ ion concentration,separately. The optimized microbial processing condition by iron-oxidizing bacteria was pH2.5,bioleaching time of 27 days,the particle size 75 μm of Realgar,the slurry concentration of 2.5 %(w/v)and Fe2+ ion concentration of 1 g·L-1. The optimized microbial processing condition was estabilished by iron-oxidizing bacteria,which could be used to research the processed products of Realgar.
引文
[1]翁雪萍,洪美华.雄黄中三氧化二砷盐的检查及含量测定方法概述[J].中国药师,2008,11(9):1117-1118.
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[6]张爽,晏磊,陈志宝,等.雄黄注射液安全性研究[J].药物评价研究,2012,35(6):451-453.
[7]张景红,李红玉.高效毛细管电泳法测定雄黄微生物炮制液中砷的形态成分[J].时珍国医国药,2011,22(2):41-46.
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[10]刘红昌,夏金兰.硫化矿生物浸出过程中界面物质形态转化及硫活化机制研究[C]//全国矿物科学与工程学术研讨会. 2016.
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[15]张爽,晏磊,陈志宝.硫氧化菌的地域分布特征[J].黑龙江八一农垦大学学报,2017,29(2):68-73.
[16]邹燕,徐文彬,宾丽英,等.两种优势菌浸矿机理及动力学研究概况[J].矿业工程,2007,5(4):65-68.
[17] Shuang Z,Lei Y,Wei D,et al. Analysis of arsenic species in realgar bioleaching solution by capillary zone electrophore sis[J].Africn Journal of Microbiology Research,2014(51):3976-3985.
[18]陈朋.氧化亚铁硫杆菌生物浸出雄黄系统研究[D].兰州:兰州大学,2011.
[19]田香,玉华,白玉霞.HPLC法测定蒙药如达七味散中栀子苷的含量[J].内蒙古民族大学学报,2016(2):152-155.
[20]金东春.黄芪复方中草药饲料添加剂对东北黑脚麻鸡免疫功能的影响[J].延边大学农学学报,2017(4):90-95.
[21]张景红,张红丽,李红玉.毛细管电泳法分离分析雄黄微生物炮制液中砷的形态成分[C]//全国离子色谱学术报告会. 2008.
[2]张志杰,周群,尉京志,等.我国药用雄黄的晶体结构鉴定[J].光谱学与光谱分析,2011,31(2):23-27.
[3]晏磊,张爽,陈志宝,等.雄黄注射液体内外抗肿瘤作用研究[J].黑龙江医药,2012,25(2):227-229.
[4]罗雅琴,徐瑞荣.雄黄治疗白血病机制研究进展[J].吉林中医药,2011,31(7):703-706.
[5]钟萌.雄黄炮制降毒方法的研究进展[J].中国药房,2007,18(33):2633-2635.
[6]张爽,晏磊,陈志宝,等.雄黄注射液安全性研究[J].药物评价研究,2012,35(6):451-453.
[7]张景红,李红玉.高效毛细管电泳法测定雄黄微生物炮制液中砷的形态成分[J].时珍国医国药,2011,22(2):41-46.
[8]张景红,樊秦,李红玉.雄黄微生物溶解液药物动力学研究[J].药学学报,2010,45(10):1279-1284.
[9]张爽,晏磊,崔玉东,等.雄黄微生物浸提液稳定性研究[J].黑龙江八一农垦大学学报,2013,25(1):45-49.
[10]刘红昌,夏金兰.硫化矿生物浸出过程中界面物质形态转化及硫活化机制研究[C]//全国矿物科学与工程学术研讨会. 2016.
[11]邓敬石,温健康,孙雪南,等.中等嗜热菌浸出硫化矿的研究现状及展望[J].矿产综合利用,2002,23(4):33-35.
[12]董浩,王红丽,王莘.降胆固醇乳酸菌的筛选、鉴定及与中药协同作用的研究[J].长春大学学报,2016(10):53-58.
[13] Goebel BM,Stackebrandt E. Cultural and phylogenetical analysis of mixed microbial populations found in natural and commercial bioleaching environments[J].Appl Environ Microbiol,1994,60:1614-1621.
[14] Tuovinen OH,Bhatti AM,Bigham JM,et a1. Oxidative dissolution of arsenopyrite by mesophilic and moderately hemophilic acidophilus[J].Appl Environ Microbiol,1994,60:3268-3274.
[15]张爽,晏磊,陈志宝.硫氧化菌的地域分布特征[J].黑龙江八一农垦大学学报,2017,29(2):68-73.
[16]邹燕,徐文彬,宾丽英,等.两种优势菌浸矿机理及动力学研究概况[J].矿业工程,2007,5(4):65-68.
[17] Shuang Z,Lei Y,Wei D,et al. Analysis of arsenic species in realgar bioleaching solution by capillary zone electrophore sis[J].Africn Journal of Microbiology Research,2014(51):3976-3985.
[18]陈朋.氧化亚铁硫杆菌生物浸出雄黄系统研究[D].兰州:兰州大学,2011.
[19]田香,玉华,白玉霞.HPLC法测定蒙药如达七味散中栀子苷的含量[J].内蒙古民族大学学报,2016(2):152-155.
[20]金东春.黄芪复方中草药饲料添加剂对东北黑脚麻鸡免疫功能的影响[J].延边大学农学学报,2017(4):90-95.
[21]张景红,张红丽,李红玉.毛细管电泳法分离分析雄黄微生物炮制液中砷的形态成分[C]//全国离子色谱学术报告会. 2008.