脉络通颗粒HPLC指纹图谱研究
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摘要
目的建立脉络通颗粒高效液相(HPLC)指纹图谱,并采用三重四极杆液质联用(LC-MS)系统对其化学成分进行定性分析,为脉络通颗粒的质量评价提供依据。方法采用Phenomenex Luna C_(18)(2)色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.3%甲酸溶液为流动相,梯度洗脱,流速1.0 mL/min,柱温25℃,检测波长270 nm。测定10批脉络通颗粒,应用中药色谱指纹图谱相似度评价系统(2004A版)建立脉络通颗粒HPLC指纹图谱,采用正、负离子模式对共有峰进行成分分析,并通过现有对照品进行确认。结果建立了脉络通颗粒HPLC指纹图谱,共标定了21个共有峰,分别归属到了8味药材,液质联用系统指认了其中19个共有峰,其中7个采用对照品对比确认。结论该方法重现性好、特征性强,能为脉络通颗粒的质量控制提供依据,并可为其他中药复方的物质基础研究提供参考。
Objective To establish a high performance liquid chromatography(HPLC) fingerprint of Mailuotong Granules,and qualitatively analyze its chemical composition by triple quadrupole liquid chromatography-mass(LC-MS) system,and provide evidence for the quality evaluation of Mailuotong Granules. Methods Phenomenex Luna C_(18)(2) column(4.6 mm × 250 mm, 5 μm) was used. Acetonitrile-0.3% formic acid solution was used as mobile phase for gradient elution. The flow rate was 1.0 mL/min, column temperature was 25℃, and the detection wavelength was 270 nm. Ten batches of Mailuotong Granules were determined. The fingerprint of Mailuotong Granules was established with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition). The common peaks were analyzed by positive and negative ion modes, and confirmed by the existing reference substances. Results The HPLC fingerprints of Mailuotong Granules were established. A total of 21 common peaks were identified and assigned to 8 herbs. The LC/MS system identified 19 common peaks, 7 of which were confirmed by comparison. Conclusion The method has good reproducibility and strong characteristics, which can provide a basis for the quality control of Mailuotong Granules and provide reference for the material basic research of other traditional Chinese medicine compounds.
Objective To establish a high performance liquid chromatography(HPLC) fingerprint of Mailuotong Granules,and qualitatively analyze its chemical composition by triple quadrupole liquid chromatography-mass(LC-MS) system,and provide evidence for the quality evaluation of Mailuotong Granules. Methods Phenomenex Luna C_(18)(2) column(4.6 mm × 250 mm, 5 μm) was used. Acetonitrile-0.3% formic acid solution was used as mobile phase for gradient elution. The flow rate was 1.0 mL/min, column temperature was 25℃, and the detection wavelength was 270 nm. Ten batches of Mailuotong Granules were determined. The fingerprint of Mailuotong Granules was established with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2004 A edition). The common peaks were analyzed by positive and negative ion modes, and confirmed by the existing reference substances. Results The HPLC fingerprints of Mailuotong Granules were established. A total of 21 common peaks were identified and assigned to 8 herbs. The LC/MS system identified 19 common peaks, 7 of which were confirmed by comparison. Conclusion The method has good reproducibility and strong characteristics, which can provide a basis for the quality control of Mailuotong Granules and provide reference for the material basic research of other traditional Chinese medicine compounds.
引文
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[2]郭学文.脉络通治疗椎基底动脉供血不足性眩晕的临床研究[J].齐齐哈尔医学院学报,2014,35(23):3484-3485.
[3]祖若玉,翟燕,赵沈娟,等.HPLC法测定脉络通颗粒中葛根素含量[J].中国药师,2013,13(2):255-256.
[4]赵沈娟,石晶萍,祖若玉,等.顶空气相色谱法测定脉络通颗粒中大孔树脂有机残留物[J].药物分析杂志,2009,29(3):458-461.
[5]王伟,杨素德,李家春,等.银翘清热片HPLC指纹图谱研究[J].中草药,2016,47(11):1882-1889.
[6]周菲菲,王彬杰,张元媛,等.冠心舒通胶囊HPLC指纹图谱研究[J].中草药,2016,47(7):1137-1141.
[7]韦辉,刘素香,刘毅,等.强肝胶囊HPLC指纹图谱研究[J].中草药,2013,44(7):845-850.
[8]常安,王维宁,杨燕云,等.透骨草药材UPLC指纹图谱研究[J].中草药,2016,47(5):832-836.
[9]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3014.
[10]陈林伟,秦昆明,徐雪松,等.中药指纹图谱数据库的研究现状及展望[J].中草药,2014,45(21):3041-3047.
[11]孙政华,邵晶,郭玫.党参化学成分及药理作用研究进展[J].安微农业科学,2015,33(57):174-176.
[12]冯佩佩,李忠祥,原忠.党参属药用植物化学成分和药理研究进展[J].沈阳药科大学学报,2012,4(5):307-311.
[13]李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,6(49):1023-1028.
[14]杜航,孙佳明,郭晓庆,等.地龙的化学成分及药理作用[J].吉林中医药,2014,7(20):707-709.
[15]李巧玉,刘杨,包华音,等.近5年丹参化学成分及药理作用研究进展[J].食品与药品,2014,16(2):145-146.
[16]瞿城,乐世俊,林航,等.红花化学成分研究[J].中草药,2015,46(13):1872-1877.
[17]朴惠顺,金光洙.木贼的化学成分和药理作用研究进展[J].时珍国医国药,2006,17(6):1077-1078.
[18]李国辉,张庆文,王一涛.葛根的化学成分研究[J].中国中药杂志,2011,35(23):3156-3160.
[19]刘丽丽,李晓霞,陈玥.槐米化学成分研究I[J].天津中医药大学学报,2014,33(4):230-233.
[20]楼陆军,罗洁霞,高云.山楂的化学成分和药理作用研究概述[J].中国药业,2014,23(3):92-94.
[21]金玉青,洪远林,李建蕊,等.川芎的化学成分及药理作用研究进展[J].中药与临床,2013,4(3):44-48.