长丝裂腹鱼温和气单胞菌的分离鉴定及药敏检测
|
摘要
2017年4月,四川省西昌某养殖场养殖的长丝裂腹鱼暴发一种以体表、内脏器官出血及角膜浑浊为特征的传染病。为探究此次疫病的病因,自病鱼的肝、脾和肾组织中分离到1株病原菌LXP170412,在脑心浸液琼脂上28℃培养48h,形成白色、半透明、表面光滑的圆形菌落,人工感染试验证实了其病原性;通过表型特征特性测定、16SrDNA和gyrB基因序列分析鉴定,分离菌LXP170412为温和气单胞菌。药敏试验结果表明,温和气单胞菌LXP170412对强力霉素、环丙沙星、氧氟沙星、诺氟沙星等敏感,对头孢拉定、新霉素、阿莫西林、庆大霉素、丁胺卡那、恩诺沙星、青霉素、罗红霉素等耐药。试验证实,温和气单胞菌为此次流行病的病原菌,药敏试验结果将为临床用药提供参考依据。
A serious infectious disease characterized by nubecula,hemorrhage in body surface and internal organs was found in Schizothorax dolichonemain a farm in Xichang,Sichuan Province in April 2017,and apathogenic bacterium(LXP170412)was isolated from the liver,spleen and kidney tissues of the diseased fish to explore the causes of the epidemic.The phenotype of the colonies cultured at 28 ℃for 24 hwas white,translucent,smooth-surface and circular in brain heart infusion(BHI).Artificial infection proved that the isolate was the pathogen of the disease,and identified as Aeromonas sobria by physicochemical properties,and sequence analysis of the 16 SrDNA and gyrB gene.The sensitivity test revealed that the strain was sensitive to doxycycline,ciprofloxacin,ofloxacin,norfloxacin and erythromycin,and resistance to cefazolin,florfenicol,amoxicillin,lincomycin,streptomycin,gentamicin,kanamycin and enrofloxacin.The findings confirmed that A.sobria was the cause of this epidemic,and provided reference data for rationally clinical application of these drugs.
A serious infectious disease characterized by nubecula,hemorrhage in body surface and internal organs was found in Schizothorax dolichonemain a farm in Xichang,Sichuan Province in April 2017,and apathogenic bacterium(LXP170412)was isolated from the liver,spleen and kidney tissues of the diseased fish to explore the causes of the epidemic.The phenotype of the colonies cultured at 28 ℃for 24 hwas white,translucent,smooth-surface and circular in brain heart infusion(BHI).Artificial infection proved that the isolate was the pathogen of the disease,and identified as Aeromonas sobria by physicochemical properties,and sequence analysis of the 16 SrDNA and gyrB gene.The sensitivity test revealed that the strain was sensitive to doxycycline,ciprofloxacin,ofloxacin,norfloxacin and erythromycin,and resistance to cefazolin,florfenicol,amoxicillin,lincomycin,streptomycin,gentamicin,kanamycin and enrofloxacin.The findings confirmed that A.sobria was the cause of this epidemic,and provided reference data for rationally clinical application of these drugs.
引文
[1]钟有光.气单胞菌及其感染流行状况[J].医学信息旬刊,2011,24(3):1233-1234.
[2]肖艳翼,王斌,夏永涛,等.鲟病原性温和气单胞菌的分离鉴定及药敏试验[J].南方农业学报,2015,46(10):1909-1914.
[3]乔国平,钱建华.温和气单胞菌引起感染性腹泻暴发调查分析[J].河南医学高等专科学校学报,2016,28(6):510-512.
[4]王根春,耿际泉,白庆燕,等.两种气单胞菌致创伤混合感染一例[J].检验医学,1994,9(4):252.
[5] Sarasqueta A O R,Garcíairure A O J,Ortega A O M,et al. Meningitis by Aeromonas sobria following cranio-encephalic traumatism[J].Infectious Diseases and Clinical Microbiology,1998,16(10):491-492.
[6] Li Y,Cai S H.Identification and pathogenicity of Aeromonas sobria on tail-rot disease in juvenile tilapia Oreochromis niloticus[J].Current Microbiology,2011,62(2):623-627.
[7] Dar G H,Dar S A,Kamili A N,et al.Detection and characterization of potentially pathogenic Aeromonas sobria isolated from fish Hypophthalmichthys molitrix(Cypriniformes:Cyprinidae)[J].Microbial Pathogenesis,2016(91):136-140.
[8]汪沐,周凤建,张天虎,等.黄颡鱼温和气单胞菌的分离鉴定与中草药药敏试验[J].科学养鱼,2017(6):63-64.
[9]张晓君,阎斌伦,邴旭文,等.异育银鲫病原温和气单胞菌表型及分子鉴定与溶血素基因检测[J].水生态学杂志,2010,3(4):102-107.
[10]张晓君,邴旭文,姚东瑞,等.泥鳅溃疡病及病原温和气单胞菌生物学及分子特征研究[J].水产科学,2010,29(12):696-702.
[11]刘敏,韩英.鲤鱼温和气单胞菌的分离鉴定和药敏试验[J].东北农业大学学报,2005,36(4):486-489.
[12]苏应兵,邹桂伟,袁科平,等.斑点叉尾鮰暴发性败血症病原的分离与鉴定[J].淡水渔业,2006,36(5):37-41.
[13]马有智,舒妙安.中华鳖温和气单胞菌病的病原研究[J].浙江大学学报:理学版,2000,27(3):329-333.
[14]叶雪平,顾金华.牛蛙温和气单胞菌病病原及防治[J].上海海洋大学学报,2000,9(1):78-80.
[15]白富瑾,唐毅,王舒正,等.大鲵温和气单胞菌的药物敏感性试验[J].水产科技情报,2014,41(6):296-301.
[16]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:106-119.
[17]Jensen S,Bergh O,Enger O,et al.Use of PCR-RFLP for genotyping 16SrRNA and characterizing bacteria cultured from halibut fry[J].Canadian Journal of Microbiology,2002,48(5):379-386.
[18]Yamamoto S,Harayama S.PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J].Appl Environ Microbiol,1995,61(3):1104-1109.
[19]徐敬钧,耿毅,汪开毓,等.似鲶高原鳅温和气单胞菌的分离鉴定及其感染的病理损伤[J].四川动物,2014,33(5):708-714.
[20]韩继卫,罗文,郑大恒.黄颡鱼温和气单胞菌的分离鉴定与药敏试验[J].绍兴文理学院学报,2011,31(9):19-21.
[21]巴翠玉,张雅斌,张培军,等.大麻哈鱼温和气单胞菌的分离鉴定及药敏试验[J].中国兽医科学,2016(6):775-780.
[22]Wahli T,Burr S E,Pugovkin D,et al.Aeromonas sobria,a causative agent of disease in farmed perch,Perca fluviatilis L[J].Journal of Fish Diseases,2005,28(3):141-150.
[23]童桂香,韦信贤,黎小正,等.黄沙鳖温和气单胞菌的分离鉴定及药敏试验[J].广东农业科学,2010,37(12):124-126.
[24]马志宏,杨慧,李铁梁,等.西伯利亚鲟(Acipenser baerii)致病性维氏气单胞菌的分离鉴定[J].微生物学报,2009,49(10):1289-1294.
[25]龚倩,高淑琴,单晓枫,等.框镜鲤致病性维氏气单胞菌的分离鉴定[J].中国预防兽医学报,2010,32(12):981-983.
[26]黎炯,叶星,卢迈新,等.罗非鱼维氏气单胞菌的分离鉴定和药敏试验[J].水生态学杂志,2011,32(3):132-136.
[27]郭闯,朱国强,王永坤,等.14株患病水生动物气单胞菌属细菌的分离鉴定与最佳治疗药物筛选试验[J].水产科学,2003,22(4):14-17.
[28]贺文旭,毛会丽,杨利敏,等.豫北地区主要淡水鱼类感染嗜水气单胞菌的流行病学调查[J].水产科学,2016,35(3):278-283.
[29]赵贤亮,陈鹤,杨潇,等.黄河鲤嗜水气单胞菌的分离、毒力及用药分析[J].水产科学,2017,36(5):642-646.
[30]蔡完其,孙佩芳.罗非鱼温和气单胞菌病的病原研究和药敏试验[J].中国水产科学,2002,9(3):243-246.
[31]胡琳琳,房文红,梁思成,等.金鱼温和气单胞菌的分离鉴定及药敏试验[J].上海海洋大学学报,2008,17(3):285-290.
[32]杜宗君,黄小丽,汪开毓,等.齐口裂腹鱼溃疡病病原的分离鉴定[J].四川农业大学学报,2011,29(2):274-279.
[33]耿毅,余泽辉,邓龙君,等.短须裂腹鱼感染迟缓爱德华氏菌的诊断与治疗[J].科学养鱼,2017(9):65.
[34] Majtn J,Jaroslaverny,Ofkan A,et al.Mortality of therapeutic fish Garra rufacaused by Aeromonas sobria[J].Asian Pac J Trop Biomed,2012,2(2):85-87.
[2]肖艳翼,王斌,夏永涛,等.鲟病原性温和气单胞菌的分离鉴定及药敏试验[J].南方农业学报,2015,46(10):1909-1914.
[3]乔国平,钱建华.温和气单胞菌引起感染性腹泻暴发调查分析[J].河南医学高等专科学校学报,2016,28(6):510-512.
[4]王根春,耿际泉,白庆燕,等.两种气单胞菌致创伤混合感染一例[J].检验医学,1994,9(4):252.
[5] Sarasqueta A O R,Garcíairure A O J,Ortega A O M,et al. Meningitis by Aeromonas sobria following cranio-encephalic traumatism[J].Infectious Diseases and Clinical Microbiology,1998,16(10):491-492.
[6] Li Y,Cai S H.Identification and pathogenicity of Aeromonas sobria on tail-rot disease in juvenile tilapia Oreochromis niloticus[J].Current Microbiology,2011,62(2):623-627.
[7] Dar G H,Dar S A,Kamili A N,et al.Detection and characterization of potentially pathogenic Aeromonas sobria isolated from fish Hypophthalmichthys molitrix(Cypriniformes:Cyprinidae)[J].Microbial Pathogenesis,2016(91):136-140.
[8]汪沐,周凤建,张天虎,等.黄颡鱼温和气单胞菌的分离鉴定与中草药药敏试验[J].科学养鱼,2017(6):63-64.
[9]张晓君,阎斌伦,邴旭文,等.异育银鲫病原温和气单胞菌表型及分子鉴定与溶血素基因检测[J].水生态学杂志,2010,3(4):102-107.
[10]张晓君,邴旭文,姚东瑞,等.泥鳅溃疡病及病原温和气单胞菌生物学及分子特征研究[J].水产科学,2010,29(12):696-702.
[11]刘敏,韩英.鲤鱼温和气单胞菌的分离鉴定和药敏试验[J].东北农业大学学报,2005,36(4):486-489.
[12]苏应兵,邹桂伟,袁科平,等.斑点叉尾鮰暴发性败血症病原的分离与鉴定[J].淡水渔业,2006,36(5):37-41.
[13]马有智,舒妙安.中华鳖温和气单胞菌病的病原研究[J].浙江大学学报:理学版,2000,27(3):329-333.
[14]叶雪平,顾金华.牛蛙温和气单胞菌病病原及防治[J].上海海洋大学学报,2000,9(1):78-80.
[15]白富瑾,唐毅,王舒正,等.大鲵温和气单胞菌的药物敏感性试验[J].水产科技情报,2014,41(6):296-301.
[16]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:106-119.
[17]Jensen S,Bergh O,Enger O,et al.Use of PCR-RFLP for genotyping 16SrRNA and characterizing bacteria cultured from halibut fry[J].Canadian Journal of Microbiology,2002,48(5):379-386.
[18]Yamamoto S,Harayama S.PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J].Appl Environ Microbiol,1995,61(3):1104-1109.
[19]徐敬钧,耿毅,汪开毓,等.似鲶高原鳅温和气单胞菌的分离鉴定及其感染的病理损伤[J].四川动物,2014,33(5):708-714.
[20]韩继卫,罗文,郑大恒.黄颡鱼温和气单胞菌的分离鉴定与药敏试验[J].绍兴文理学院学报,2011,31(9):19-21.
[21]巴翠玉,张雅斌,张培军,等.大麻哈鱼温和气单胞菌的分离鉴定及药敏试验[J].中国兽医科学,2016(6):775-780.
[22]Wahli T,Burr S E,Pugovkin D,et al.Aeromonas sobria,a causative agent of disease in farmed perch,Perca fluviatilis L[J].Journal of Fish Diseases,2005,28(3):141-150.
[23]童桂香,韦信贤,黎小正,等.黄沙鳖温和气单胞菌的分离鉴定及药敏试验[J].广东农业科学,2010,37(12):124-126.
[24]马志宏,杨慧,李铁梁,等.西伯利亚鲟(Acipenser baerii)致病性维氏气单胞菌的分离鉴定[J].微生物学报,2009,49(10):1289-1294.
[25]龚倩,高淑琴,单晓枫,等.框镜鲤致病性维氏气单胞菌的分离鉴定[J].中国预防兽医学报,2010,32(12):981-983.
[26]黎炯,叶星,卢迈新,等.罗非鱼维氏气单胞菌的分离鉴定和药敏试验[J].水生态学杂志,2011,32(3):132-136.
[27]郭闯,朱国强,王永坤,等.14株患病水生动物气单胞菌属细菌的分离鉴定与最佳治疗药物筛选试验[J].水产科学,2003,22(4):14-17.
[28]贺文旭,毛会丽,杨利敏,等.豫北地区主要淡水鱼类感染嗜水气单胞菌的流行病学调查[J].水产科学,2016,35(3):278-283.
[29]赵贤亮,陈鹤,杨潇,等.黄河鲤嗜水气单胞菌的分离、毒力及用药分析[J].水产科学,2017,36(5):642-646.
[30]蔡完其,孙佩芳.罗非鱼温和气单胞菌病的病原研究和药敏试验[J].中国水产科学,2002,9(3):243-246.
[31]胡琳琳,房文红,梁思成,等.金鱼温和气单胞菌的分离鉴定及药敏试验[J].上海海洋大学学报,2008,17(3):285-290.
[32]杜宗君,黄小丽,汪开毓,等.齐口裂腹鱼溃疡病病原的分离鉴定[J].四川农业大学学报,2011,29(2):274-279.
[33]耿毅,余泽辉,邓龙君,等.短须裂腹鱼感染迟缓爱德华氏菌的诊断与治疗[J].科学养鱼,2017(9):65.
[34] Majtn J,Jaroslaverny,Ofkan A,et al.Mortality of therapeutic fish Garra rufacaused by Aeromonas sobria[J].Asian Pac J Trop Biomed,2012,2(2):85-87.