中药药效物质的研究方法
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摘要
中药药效物质的研究是中药现代化研究的核心,科学阐明中药临床治疗的物质基础,是中药药效学研究的最终目标。现阶段中药药效物质基础研究方法主要有化学分离分析、生物色谱法和虚拟筛选等方法。本文对现有中药药效物质基础的几种研究方法进行了分类,并对其特点进行了介绍。
The research of pharmacophore substance of traditional Chinese medicine(TCM) is the core of modernization of TCM. The material basis of clinical treatment of TCM is clarified scientifically that is the ultimate goal of research on the pharmacodynamics of TCM. At present, the main research methods of material basis of TCM include chemical separation and analysis, biochromatography, virtual screening and so on. In this paper, several existing research methods are classified and their characteristics are introduced.
The research of pharmacophore substance of traditional Chinese medicine(TCM) is the core of modernization of TCM. The material basis of clinical treatment of TCM is clarified scientifically that is the ultimate goal of research on the pharmacodynamics of TCM. At present, the main research methods of material basis of TCM include chemical separation and analysis, biochromatography, virtual screening and so on. In this paper, several existing research methods are classified and their characteristics are introduced.
引文
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[5]于垂亮,侯惠民.蟾酥抗肿瘤有效成分的活性追踪分离及急性毒性研究.中草药,2011,42(2):307-311
[6]吕丽娟,任凯,那木汗,等.尖叶假龙胆有效成分的活性追踪分离及对H2O2诱导PC12细胞氧化损伤的保护作用研究.中草药,2017,48(10):1957-1963
[7]俞凌燕,王毅,范骁辉,等.用组分剔除法研究中药的有效组分.中国中药杂志,2009,34(3):336-339
[8]夏青.新技术、新方法在中药药效物质基础发现中的应用概述[A].中华中医药学会.中华中医药学会中药化学分会第九届学术年会论文集(第一册)[C].中华中医药学会,2014:7
[9]刘菊燕.茅苍术的血清药物化学和血清药理学研究[D].南京:南京中医药大学,2015
[10]许闪,王靓,黄金玲,等.苓桂术甘汤含药血清对TGF-β诱导的大鼠心肌细胞株H9c2凋亡的影响.中药药理与临床,2016,32(3):4-8
[11]李月婷,李靖,肖婷婷,等.基于中药血清药物化学及血清药理学方法探讨荭草保护心肌细胞氧化损伤的物质基础.中国实验方剂学杂志,2013,19(2):158-162
[12]杨文宇,万德光,杨鑫嵎.虚拟筛选辅助揭示中药药效物质基础的思路与初步实践.中草药,2011,42(9):1665-1672
[13]严一文,徐培平,黄新安,等.基于计算机虚拟筛选的赤芍抗流感作用物质基础研究.中药材,2017,40(2):430-435
[14]陈梅妹,杨雪梅,赖新梅,等.清眩降压汤治疗高血压作用机制的虚拟筛选研究.中华中医药杂志,2016,31(04):1471-1474
[15]Moy TI,Ball AR,Ausubel FM,et al.From the cover identification of novel antimicrobials using a live-animal infection model.Proc Nat Acad Sci USA,2006,103(27):10414-10419
[16]辛胜昌,赵艳秋,李松,等.斑马鱼模型在药物筛选中的应用.遗传,2012,34(9):1144-1152
[17]冯康虎,方鹏飞,张文贤,等.斑马鱼模型及其在血管生成抑制剂筛选中的应用.西部中医药,2014,27(12):141-144
[18]张君,金亚,叶燕锐,等.G蛋白偶联受体配体结合分析技术.药物分析杂志,2015,35(1):1-7
[19]孔爱英,乔晋萍,武仙英,等.受体-配体结合的质谱分析方法研究进展.中国药学杂志,2013,48(15):1244-1248
[20]周思维,邱瑞琪,高彩芳,等.生物色谱用于中药活性成分筛选.中国医药工业杂志,2017,48(12):1692-1697
[21]Zhang C,Li J,Xu L,et al.Fast immobilized liposome chromatography based on penetrable silica microspheres for screening and analysis of permeable compounds.JChromato A,2012,1233(7):78-84
[22]Wang S,Wang C,Zhao X,et al.Comprehensive two-dimensional high performance liquid chromatography system with immobilized liposome chromatography column and monolithic column for separation of the traditional Chinese medicine Schisandra chinensis.Anal Chim Acta,2012,713(3):121-129
[23]赵小亮.脂质体色谱及其在中药活性成分筛选和评价中的应用进展[A].中国药学会2014:8
[24]封顺.牛血清白蛋白与人血清白蛋白键合液相手性固定相的制备及其性质的研究[D].乌鲁木齐:新疆大学,2002
[25]何媛.功能蛋白与药物相互作用的亲和色谱法研究[D].西安:西北大学,2012
[26]Tong S,Fu M,Cao X,et al.Lipid Raft Stationary Phase Chromatography for Screening Anti-tumor Components from Galla chinensis.Chromatographia,2014,77(5-6):419-429
[27]Yue Y,Xue H,Wang X,et al.A high expressio1 AR/cell membrane chromatography method based on a target receptor to screen active ingredients from traditional Chinese medicine.J Sep Sci,2013,37(3):244-252
[28]杜晖,吕楠,黄洁,等.细胞膜色谱法筛选川芎-白芷中5-HT受体激动剂的研究.中国中药杂志,2015,40(3):490-494
[29]纪松岗,丁璇,曹岩,等.用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质.药学实践杂志,2014,32(06):425-427,433
[30]吕艳妮,付佳,孔利云,等.细胞膜色谱在线HPLC-IT-TOF MS联用系统筛选分析射干中次野鸢尾黄素的抗EGFR活性作用.质谱学报,2017,38(04):425-432
[31]朱建梁.细胞固相色谱法研究牡丹皮中的效应成分[D].合肥:安徽大学,2013
[32]方慧,鄢庆枇.靶向核受体RXRα的淫羊藿抗炎活性成分的快速精准鉴定.国际检验医学杂志,2017,38(23):3245-3248
[33]Liu S,Yan J,Xing J,et al.Characterization of compounds and potential neuraminidase inhibitors from the n-butanol extract of compound indigowoad root granule using ultrafiltration and liquid chromatography-tandem mass spectrometry.J Pharm Biomed Anal,2012,59(1):96-101
[34]Singh N,Ravichandran S,Spelman K,et al.The identification of a novel SIRT6 modulator from Trigonella foenum-graecum,using ligand fishing with protein coated magnetic beads.J Chromatogr B,2014,968:105-111
[35]Liu M,Fan J,Wang S,et al.Transcriptional profiling of Chinese medicinal formula Si-Wu-Tang on breast cancer cells reveals phytoestrogenic activity.BMC Complem Altern M,2013,13(1):11
[2]朱诗塔,雷鹏,李新中,等.掌叶大黄不同炮制品指纹图谱与其止血作用的灰关联度分析.中南药学,2009,7(1):55-58
[3]郭延生,华永丽,邓红娟,等.当归不同炮制品清除自由基谱效关系研究.中成药,2010,32(12):2107-2111
[4]许良,毕开顺.多元线性回归分析法在蒙药森登-4汤谱效关系解析中的应用.计算机与应用化学,2008,25(10):1189-1192
[5]于垂亮,侯惠民.蟾酥抗肿瘤有效成分的活性追踪分离及急性毒性研究.中草药,2011,42(2):307-311
[6]吕丽娟,任凯,那木汗,等.尖叶假龙胆有效成分的活性追踪分离及对H2O2诱导PC12细胞氧化损伤的保护作用研究.中草药,2017,48(10):1957-1963
[7]俞凌燕,王毅,范骁辉,等.用组分剔除法研究中药的有效组分.中国中药杂志,2009,34(3):336-339
[8]夏青.新技术、新方法在中药药效物质基础发现中的应用概述[A].中华中医药学会.中华中医药学会中药化学分会第九届学术年会论文集(第一册)[C].中华中医药学会,2014:7
[9]刘菊燕.茅苍术的血清药物化学和血清药理学研究[D].南京:南京中医药大学,2015
[10]许闪,王靓,黄金玲,等.苓桂术甘汤含药血清对TGF-β诱导的大鼠心肌细胞株H9c2凋亡的影响.中药药理与临床,2016,32(3):4-8
[11]李月婷,李靖,肖婷婷,等.基于中药血清药物化学及血清药理学方法探讨荭草保护心肌细胞氧化损伤的物质基础.中国实验方剂学杂志,2013,19(2):158-162
[12]杨文宇,万德光,杨鑫嵎.虚拟筛选辅助揭示中药药效物质基础的思路与初步实践.中草药,2011,42(9):1665-1672
[13]严一文,徐培平,黄新安,等.基于计算机虚拟筛选的赤芍抗流感作用物质基础研究.中药材,2017,40(2):430-435
[14]陈梅妹,杨雪梅,赖新梅,等.清眩降压汤治疗高血压作用机制的虚拟筛选研究.中华中医药杂志,2016,31(04):1471-1474
[15]Moy TI,Ball AR,Ausubel FM,et al.From the cover identification of novel antimicrobials using a live-animal infection model.Proc Nat Acad Sci USA,2006,103(27):10414-10419
[16]辛胜昌,赵艳秋,李松,等.斑马鱼模型在药物筛选中的应用.遗传,2012,34(9):1144-1152
[17]冯康虎,方鹏飞,张文贤,等.斑马鱼模型及其在血管生成抑制剂筛选中的应用.西部中医药,2014,27(12):141-144
[18]张君,金亚,叶燕锐,等.G蛋白偶联受体配体结合分析技术.药物分析杂志,2015,35(1):1-7
[19]孔爱英,乔晋萍,武仙英,等.受体-配体结合的质谱分析方法研究进展.中国药学杂志,2013,48(15):1244-1248
[20]周思维,邱瑞琪,高彩芳,等.生物色谱用于中药活性成分筛选.中国医药工业杂志,2017,48(12):1692-1697
[21]Zhang C,Li J,Xu L,et al.Fast immobilized liposome chromatography based on penetrable silica microspheres for screening and analysis of permeable compounds.JChromato A,2012,1233(7):78-84
[22]Wang S,Wang C,Zhao X,et al.Comprehensive two-dimensional high performance liquid chromatography system with immobilized liposome chromatography column and monolithic column for separation of the traditional Chinese medicine Schisandra chinensis.Anal Chim Acta,2012,713(3):121-129
[23]赵小亮.脂质体色谱及其在中药活性成分筛选和评价中的应用进展[A].中国药学会2014:8
[24]封顺.牛血清白蛋白与人血清白蛋白键合液相手性固定相的制备及其性质的研究[D].乌鲁木齐:新疆大学,2002
[25]何媛.功能蛋白与药物相互作用的亲和色谱法研究[D].西安:西北大学,2012
[26]Tong S,Fu M,Cao X,et al.Lipid Raft Stationary Phase Chromatography for Screening Anti-tumor Components from Galla chinensis.Chromatographia,2014,77(5-6):419-429
[27]Yue Y,Xue H,Wang X,et al.A high expressio1 AR/cell membrane chromatography method based on a target receptor to screen active ingredients from traditional Chinese medicine.J Sep Sci,2013,37(3):244-252
[28]杜晖,吕楠,黄洁,等.细胞膜色谱法筛选川芎-白芷中5-HT受体激动剂的研究.中国中药杂志,2015,40(3):490-494
[29]纪松岗,丁璇,曹岩,等.用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质.药学实践杂志,2014,32(06):425-427,433
[30]吕艳妮,付佳,孔利云,等.细胞膜色谱在线HPLC-IT-TOF MS联用系统筛选分析射干中次野鸢尾黄素的抗EGFR活性作用.质谱学报,2017,38(04):425-432
[31]朱建梁.细胞固相色谱法研究牡丹皮中的效应成分[D].合肥:安徽大学,2013
[32]方慧,鄢庆枇.靶向核受体RXRα的淫羊藿抗炎活性成分的快速精准鉴定.国际检验医学杂志,2017,38(23):3245-3248
[33]Liu S,Yan J,Xing J,et al.Characterization of compounds and potential neuraminidase inhibitors from the n-butanol extract of compound indigowoad root granule using ultrafiltration and liquid chromatography-tandem mass spectrometry.J Pharm Biomed Anal,2012,59(1):96-101
[34]Singh N,Ravichandran S,Spelman K,et al.The identification of a novel SIRT6 modulator from Trigonella foenum-graecum,using ligand fishing with protein coated magnetic beads.J Chromatogr B,2014,968:105-111
[35]Liu M,Fan J,Wang S,et al.Transcriptional profiling of Chinese medicinal formula Si-Wu-Tang on breast cancer cells reveals phytoestrogenic activity.BMC Complem Altern M,2013,13(1):11
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