N-乙酰氨基葡萄糖转移酶Ⅴ在卵巢浆液性囊腺癌中表达及临床意义
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摘要
目的探讨N-乙酰氨基葡萄糖转移酶Ⅴ(GnT-Ⅴ)在卵巢浆液性囊腺癌中的表达及临床意义。方法收集卵巢浆液性囊腺癌标本152例、卵巢浆液性囊腺瘤标本100例及卵巢交界性浆液性囊腺瘤标本20例,检测标本中GnT-Ⅴ的表达水平并分析其与卵巢浆液性囊腺癌临床病理参数的关系。结果卵巢浆液性囊腺癌GnT-Ⅴ阳性表达率25.7%(39/152)明显低于浆液性囊腺瘤67.0%(67/100)与交界性浆液性囊腺瘤45.0%(9/20);早期卵巢浆液性囊腺癌GnT-Ⅴ阳性表达率33.3%(23/69)明显高于晚期19.3%(16/83);高分化卵巢浆液性囊腺癌GnT-Ⅴ阳性表达率38.6%(22/57)明显高于中低分化17.9%(17/95),差异均有统计学意义(P<0.05)。已有淋巴结转移的卵巢浆液性囊腺癌GnT-Ⅴ阳性表达率22.9%(16/70)与无淋巴结转移的卵巢浆液性囊腺癌GnT-Ⅴ阳性表达率28.8%(23/82)比较,差异无统计学意义(P>0.05)。结论 GnT-Ⅴ在卵巢浆液性囊腺癌中表达下调,其表达水平降低可能预示着肿瘤分化程度低、预后差。
Objective To investigate the expression level and clinical significance of N-acetylglucosaminyltransferase V( GnT-Ⅴ) in ovarian serous cystadenocarcinoma. Methods A retrospective study was performed on 152 cases of ovarian serous cystadenocarcinoma,100 cases of ovarian serous cystadenoma and 20 cases of borderline ovarian serous cystadenoma. The relationship of GnT-Ⅴ expression and clinicopathologic parameters of ovarian serous cystadenocarcinoma was analyzed. Results The positive expression rate of ovarian serous cystadenocarcinoma( 25. 7%,39/152) was significantly lower than that of ovarian serous cystadenoma( 67. 0%,67/100) and borderline ovarian serous cystadenoma(45. 0%,9/20);early ovarian serous cystadenocarcinoma GnT-Ⅴ positive expression rate(33. 3%,23/69) was significantly higher than that in the late ovarian serous cystadenocarcinoma(19. 3%,16/83);high differentiation of ovarian serous cystadenocarcinoma GnT-Ⅴ positive expression rate(38. 6%,22/57) was significantly higher than that in low differentiation(17. 9%,17/95),and the difference had statistical significance( P < 0. 05). The GnT-Ⅴ positive expression rate in ovarian serous cystadenocarcinoma with lymph node metastasis was(22. 9%,16/70),and in ovarian serous cystadenocarcinoma without lymph node metastasis was(28. 8%,23/82),the difference was not statistically significant(P > 0. 05). Conclusion The expression of GnT-Ⅴ is down-regulated in ovarian serous cystadenocarcinoma,which may be correlated with low degree of differentiation and poor prognosis.
Objective To investigate the expression level and clinical significance of N-acetylglucosaminyltransferase V( GnT-Ⅴ) in ovarian serous cystadenocarcinoma. Methods A retrospective study was performed on 152 cases of ovarian serous cystadenocarcinoma,100 cases of ovarian serous cystadenoma and 20 cases of borderline ovarian serous cystadenoma. The relationship of GnT-Ⅴ expression and clinicopathologic parameters of ovarian serous cystadenocarcinoma was analyzed. Results The positive expression rate of ovarian serous cystadenocarcinoma( 25. 7%,39/152) was significantly lower than that of ovarian serous cystadenoma( 67. 0%,67/100) and borderline ovarian serous cystadenoma(45. 0%,9/20);early ovarian serous cystadenocarcinoma GnT-Ⅴ positive expression rate(33. 3%,23/69) was significantly higher than that in the late ovarian serous cystadenocarcinoma(19. 3%,16/83);high differentiation of ovarian serous cystadenocarcinoma GnT-Ⅴ positive expression rate(38. 6%,22/57) was significantly higher than that in low differentiation(17. 9%,17/95),and the difference had statistical significance( P < 0. 05). The GnT-Ⅴ positive expression rate in ovarian serous cystadenocarcinoma with lymph node metastasis was(22. 9%,16/70),and in ovarian serous cystadenocarcinoma without lymph node metastasis was(28. 8%,23/82),the difference was not statistically significant(P > 0. 05). Conclusion The expression of GnT-Ⅴ is down-regulated in ovarian serous cystadenocarcinoma,which may be correlated with low degree of differentiation and poor prognosis.
引文
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[18]Takahashi N,Yamamoto E,Ino K,et al.High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary[J].Oncol Rep,2009,22(5):1027-1032.
[2]Li N,Xu H,Fan K,et al.Alteredβ1,6-Glc NAc branched N-glycans impair TGF-β-mediated epithelial-to-mesenchymal transition through Smad signalling pathway in human lung cancer[J].J Cell Mol Med,2014,18(10):1975-1991.
[3]贺宏勇,陈伟东,汪学非,等.胃癌分化程度与β1,6 N-乙酰氨基葡萄糖转移酶V的关系[J].中华实验外科杂志,2012,29(12):2569-2571.
[4]Denny L,Quinn M.FIGO Cancer Report 2015[J].Int J Gynaecol Obstet,2015,131(2):S75.
[5]Jayson GC,Kohn EC,Kitchener HC,et al.Ovarian cancer[J].Lancet,2014,384(9951):1376-1388.
[6]Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[7]刘彦红,刘红燕,张立会,等.卵巢浆液性囊腺癌及囊腺瘤的差异蛋白质组学分析[J].中国实验诊断学,2011,15(6):1069-1073.
[8]张晓霞,孙晓琦,张松灵,等.卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义[J].吉林大学学报(医学版),2010,36(3):536-539.
[9]Takahashi M,Kizuka Y,Ohtsubo K,et al.Disease-associated glycans on cell surface proteins[J].Mol Aspects Med,2016,51:56-70.
[10]Taniguchi N,Kizuka Y.Glycans and cancer:role of N-glycans in cancer biomarker,progression and metastasis,and therapeutics[J].Adv Cancer Res,2015,126:11-51.
[11]Saito H,Gu J,Nishikawa A,et al.Organization of the human Nacetylglucosaminyltransferase V gene[J].Eur J Biochem,1995,233(1):18-26.
[12]杨娟,陈杰.N-乙酰氨基葡萄糖转移酶-V与恶性肿瘤增殖和侵袭转移的关系[J].华西医学,2014,29(9):1779-1782.
[13]Wei T,Liu Q,He F,et al.The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma[J].Exp Mol Pathol,2012,93(1):8-17.
[14]Pinho SS,Seruca R,Gartner F,et al.Modulation of E-cadherin function and dysfunction by N-glycosylation[J].Cell Mol Life Sci,2011,68(6):1011-1020.
[15]Granovsky M,Fata J,Pawling J,et al.Suppression of tumor growth and metastasis in Mgat5-deficient mice[J].Nat Med,2000,6(3):306-312.
[16]Murata K,Miyoshi E,Kameyama M,et al.Expression of Nacetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis[J].Clin Cancer Res,2000,6(5):1772-1777.
[17]Ishimura H,Takahashi T,Nakagawa H,et al.N-acetylglucosaminyltransferase V and beta1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer[J].Clin Cancer Res,2006,12(8):2506-2511.
[18]Takahashi N,Yamamoto E,Ino K,et al.High expression of N-acetylglucosaminyltransferase V in mucinous tumors of the ovary[J].Oncol Rep,2009,22(5):1027-1032.