45味中药对多重耐药大肠杆菌的抑菌效果
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摘要
目的:筛选对多重耐药大肠杆菌抑制作用较好的中药,进行组方,研发新药,探讨中药提取物或中药制剂对防治多重耐药菌株引起疾病的效果。方法:采用纸片扩散法(K-B法)检测不同来源的大肠杆菌对13种抗生素类药物的敏感性,筛选出多重耐药菌株;采用牛津杯法测定45味中药提取液对多重耐药菌的抑菌圈直径,筛选出表现为高敏的中药;采用二倍稀释法确定10味高敏中药的最小抑菌浓度,评估不同中药对多重耐药菌株的抑制效果。结果:筛选出耐药率高于70%的多重耐药菌株7株,其中猪源3株、羊源1株、鹅源3株;多重耐药菌株对28味中药表现为不敏感,对7味中药表现为中敏,对10味中药表现为高敏;抑菌效果最好的3味中药依次是博落回、黄连和水杨梅。结论:不同中药对同一多重耐药菌株抑制效果不同,一些中药对多重耐药菌可以起到较好的体外抑制效果,为研制临床上耐药性疾病防治的新药物提供参考。
Objective:To screen some Chinese medicines with strong inhibitory effect on multidrug-resistant E.coli and formulate the prescription,develop new drugs,and discuss the effect of using Chinese herbal medicine extract or Chinese herbal medicine preparation to prevent and cure the disease caused by multi-drug-resistant strains.Methods:K-B method was applied to test the susceptibility of E.coli to 13 kinds of antibiotics and screen out the multi-drug resistant strains.Oxford cup method was used to determine the inhibition zone diameter of 45 Chinese herbal extracts against multidrug-resistant bacteria and screen out highly sensitive Chinese herbs.Two fold dilution method was used to determine the minimal inhibitory concentration(MIC)of the 10 highly sensitive Chinese herbs and evaluate the inhibitory effect of different Chinese medicines on multidrug-resistant strains.Results:A total of 7 strains of multidrug-resistant strains with a resistance rate of more than 70% were screened out.Among them,3 strains were from pigs,1 from sheep and 3 from goose.Multidrug-resistant strains showed resistance to 28 Chinese medicines,medium sensitivity to 7 Chinese herbs and highly sensitive to 10 herbs.The best 3 antibacterial herbs were Macleaya cordata,Coptis chinensis and Myrica rubra.Conclusion:Different Chinese medicines have different inhibitory effects on the same multidrug-resistant bacteria.Some Chinese medicines exhibit better inhibitory effects on multidrug-resistant bacteria in vitro,which provides a basis for the development of new drugs for the prevention and treatment of clinical drug-resistant diseases.
Objective:To screen some Chinese medicines with strong inhibitory effect on multidrug-resistant E.coli and formulate the prescription,develop new drugs,and discuss the effect of using Chinese herbal medicine extract or Chinese herbal medicine preparation to prevent and cure the disease caused by multi-drug-resistant strains.Methods:K-B method was applied to test the susceptibility of E.coli to 13 kinds of antibiotics and screen out the multi-drug resistant strains.Oxford cup method was used to determine the inhibition zone diameter of 45 Chinese herbal extracts against multidrug-resistant bacteria and screen out highly sensitive Chinese herbs.Two fold dilution method was used to determine the minimal inhibitory concentration(MIC)of the 10 highly sensitive Chinese herbs and evaluate the inhibitory effect of different Chinese medicines on multidrug-resistant strains.Results:A total of 7 strains of multidrug-resistant strains with a resistance rate of more than 70% were screened out.Among them,3 strains were from pigs,1 from sheep and 3 from goose.Multidrug-resistant strains showed resistance to 28 Chinese medicines,medium sensitivity to 7 Chinese herbs and highly sensitive to 10 herbs.The best 3 antibacterial herbs were Macleaya cordata,Coptis chinensis and Myrica rubra.Conclusion:Different Chinese medicines have different inhibitory effects on the same multidrug-resistant bacteria.Some Chinese medicines exhibit better inhibitory effects on multidrug-resistant bacteria in vitro,which provides a basis for the development of new drugs for the prevention and treatment of clinical drug-resistant diseases.
引文
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[13] 张代玉,周庆民,徐馨,等.中药对奶牛乳房炎主要病原菌体外抑菌试验效果的研究[J].黑龙江畜牧兽医,2016,3(5):206-208.
[14] 刘辉.中药组方对猪大肠杆菌病抑菌效果观察[J].中国畜禽种业,2017,13(5):101.
[15] 李晏乐,岳肖华,孙卫东.中草药抑菌作用的现代研究进展[J].中国中医基础医学杂志,2018,24(6):860-862.
[16] 李蕴玉,李佩国,张召兴,等.单味中药水提物对鸡源耐药性E.coli地方株的体外抑菌活性研究[J].中国兽医杂志,2018,54(4):47-49.
[17] 蔡香菊,马骏,王志晓,等.黄芩等中药及复方制剂对多重耐药鲍曼不动杆菌体外抑菌活性研究[J].中兽医医药杂志,2018,37(1):8-10.
[18] 毛红梅.几种中药颗粒剂与水煎剂对不同种类耐药菌株的体外抑菌作用比较[J].当代医学,2017,23(19):73-74.
[19] 芦亚君,程宁.3种中药方剂逆转大肠杆菌耐药性的实验观察[J].西北药学杂志,2007,22(6)309-311.
[20] 牛志伟,范华龙,吕小丽,等.4种中药对嗜水气单胞菌抑菌效果的Meta分析[J].南方农业学报,2017,48(1):140-150.
[21] HUANG X L,LIU R J,WHYTE S,et al.The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.[J].J Appl Ichthyol,2015,31(4):681-686.
[22] LAU C,MOOIMAN K D,MAAS-BAKKER R F,et al.Meijerman.Effect of Chinese herbs on CYP3A4 activity and expression in vitro[J].J Ethnopharmacol,2013,149(2):543-549.
[23] 邓婷,金彦.14种中药对奶牛子宫内膜炎主要致病菌的体外抑菌试验[J].湖南畜牧兽医,2018,19(1):38-41.
[24] 李蕾,谭文颖,杨博,等.柚子叶等5种中药对鸡粪中大肠杆菌的体外抑菌试验[J].广东化工,2017,44(3):28-29,22.
[2] YEPEZ E S,BOVERA M M,ROSENTHAL V D,et al.Device-associated infection rates,mortality,length of stay and bacterial resistance in intensive care units in Ecuador:International Nosocomial Infection Control Consortium’s findings[J].World J Biol chem,2017,8(1):95-101.
[3] 伊风军.抗生素添加剂在饲料中的应用历史和现状[J].中国畜牧兽医文摘,2017,33(11):221.
[4] WU M,DENG J P,YAO C H,et al.Extended-spectrum β-lactamases producingKlebsiella pneumoniae and Escherichia coli:distribution and drug-Resistance analysis[J].Chin J Nosocomiol,2015,17(2):454.
[5] 李海华,郭蔚冰,杨海明,等.内蒙古中东部地区不同来源大肠杆菌耐药性调查[J].中国畜牧兽医,2017,44(4):1182-1188.
[6] 马存,冼少华,相雨,等.肉豆蔻药理作用研究进展[J].中国现代中药,2017,19(8):1200-1206.
[7] LIU J H,LI L,SHANG X D,et al.Anti-Helicobacter pylori activity of bioactive components isolated from Hericium erinaceus[J].Journal of Ethnopharmacology,2016,183(5):54-58.
[8] 曾建国.博落回资源为示范的中药材全产业链研究与综合利用[J].中国现代中药,2017,19(10):1359-1366.
[9] 朴喜航,艾红佳.中药抗菌成分及其抗菌机制的研究进展[J].吉林医药学院学报,2017,38(6):445-447.
[10] 陈静,林海英,杨霞卿,等.11味中药对痤疮丙酸杆菌的体外抑制作用研究[J].药学与临床研究,2018,26(3):187-189.
[11] 陈希文,赖守勋,尹苗,等.22种中药多糖对猪源金黄色葡萄球菌的体外抑菌活性[J].江苏农业科学,2016,44(9):264-267.
[12] 王帅兵,王婧,陈海峰,等.8种中药对猪源大肠杆菌的体外抑菌效果[J].贵州农业科学,2018,46(4):94-97.
[13] 张代玉,周庆民,徐馨,等.中药对奶牛乳房炎主要病原菌体外抑菌试验效果的研究[J].黑龙江畜牧兽医,2016,3(5):206-208.
[14] 刘辉.中药组方对猪大肠杆菌病抑菌效果观察[J].中国畜禽种业,2017,13(5):101.
[15] 李晏乐,岳肖华,孙卫东.中草药抑菌作用的现代研究进展[J].中国中医基础医学杂志,2018,24(6):860-862.
[16] 李蕴玉,李佩国,张召兴,等.单味中药水提物对鸡源耐药性E.coli地方株的体外抑菌活性研究[J].中国兽医杂志,2018,54(4):47-49.
[17] 蔡香菊,马骏,王志晓,等.黄芩等中药及复方制剂对多重耐药鲍曼不动杆菌体外抑菌活性研究[J].中兽医医药杂志,2018,37(1):8-10.
[18] 毛红梅.几种中药颗粒剂与水煎剂对不同种类耐药菌株的体外抑菌作用比较[J].当代医学,2017,23(19):73-74.
[19] 芦亚君,程宁.3种中药方剂逆转大肠杆菌耐药性的实验观察[J].西北药学杂志,2007,22(6)309-311.
[20] 牛志伟,范华龙,吕小丽,等.4种中药对嗜水气单胞菌抑菌效果的Meta分析[J].南方农业学报,2017,48(1):140-150.
[21] HUANG X L,LIU R J,WHYTE S,et al.The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.[J].J Appl Ichthyol,2015,31(4):681-686.
[22] LAU C,MOOIMAN K D,MAAS-BAKKER R F,et al.Meijerman.Effect of Chinese herbs on CYP3A4 activity and expression in vitro[J].J Ethnopharmacol,2013,149(2):543-549.
[23] 邓婷,金彦.14种中药对奶牛子宫内膜炎主要致病菌的体外抑菌试验[J].湖南畜牧兽医,2018,19(1):38-41.
[24] 李蕾,谭文颖,杨博,等.柚子叶等5种中药对鸡粪中大肠杆菌的体外抑菌试验[J].广东化工,2017,44(3):28-29,22.