QuEChERS-气相色谱法检测鳞茎类蔬菜中多种有机磷农药残留的方法优化
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摘要
根据2018年国家食品安全监督抽检实施细则,对于鳞茎类蔬菜,以大葱为例,需要检测的有机磷农药有19种。本文利用Qu ECh ERS进行前处理优化,利用气相色谱仪快速测定鳞茎类蔬菜中的18种有机磷农药(甲胺磷、乙酰甲胺磷、氧乐果、乐果、甲基对硫磷、倍硫磷、对硫磷、甲基硫环磷、硫环磷、灭线磷、甲拌磷、二嗪磷、久效磷、马拉硫磷、杀螟硫磷、甲基异柳磷、水胺硫磷和杀扑磷)。Qu ECh ERS前处理使用乙腈作为萃取剂,无水硫酸镁用于除去基质中的水分,QuEChERS方法中最佳配比是55 mg C18、55 mg PSA、55 mg GCB,能提高农药的提取效率,方法重复性好,为检测机构提供了一种简便、快速的农药多残留分析方法。
According to a 2018 national food safety supervision and sampling rules, bulb vegetables green Chinese onion, for example, the need to detect organophosphorus pesticide has 19 kinds. In this paper, using the QuEChERS pretreatment optimization, using gas chromatograph, rapid determination of 18 organophosphorus pesticides in vegetables bulbs(methamidophos, acephate, oxygen dimethoate, dimethoate, methyl parathion, fenthion, parathion methyl sulfur phosphorus, sulfur, ring ring, destroy the line p, phorate, diazinon monocrotophos, marla sulfur phosphorus, fenitrothion,methyl isopropyl willow phosphorus, isocarbophos and methidathion). Qu ECh ERS pretreatment using acetonitrile as extraction agent, anhydrous magnesium sulfate is used to remove moisture from the substrate, Qu ECh ERS method is optimal proportion of 55 mg C18, 55 mg PSA, 55 mg GCB, improved the extraction efficiency of pesticide, the method repeatability, provides an easy and rapid for testing institutions of pesticide residue analysis method.
According to a 2018 national food safety supervision and sampling rules, bulb vegetables green Chinese onion, for example, the need to detect organophosphorus pesticide has 19 kinds. In this paper, using the QuEChERS pretreatment optimization, using gas chromatograph, rapid determination of 18 organophosphorus pesticides in vegetables bulbs(methamidophos, acephate, oxygen dimethoate, dimethoate, methyl parathion, fenthion, parathion methyl sulfur phosphorus, sulfur, ring ring, destroy the line p, phorate, diazinon monocrotophos, marla sulfur phosphorus, fenitrothion,methyl isopropyl willow phosphorus, isocarbophos and methidathion). Qu ECh ERS pretreatment using acetonitrile as extraction agent, anhydrous magnesium sulfate is used to remove moisture from the substrate, Qu ECh ERS method is optimal proportion of 55 mg C18, 55 mg PSA, 55 mg GCB, improved the extraction efficiency of pesticide, the method repeatability, provides an easy and rapid for testing institutions of pesticide residue analysis method.
引文
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[3]叶宇飞,许秀琴,王立君,等.气相色谱法测定蔬菜水果中农药残留的基质效应研究[J].安徽农学通报,2015(5):114-116.
[4]高青珍.QuEChERS-色谱法快速测定蔬菜水果中的农药多残留[D].北京:中国农业科学院,2014.
[5]Diez C,Traag W A,Zommer P,et al.Comparison of An Acetonitrile Extraction/partitioning and“Dispersive Solid-phase Extraction”Method with Classical Multiresidue Methods for the Extraction of Herbicide Residues in Barley Samples[J].Journal of Chromatography A,2006,1131(1-2):11-23.
[6]黄雪松.大葱挥发油含量与化学成分的分析[J].食品与发酵工业,2004,30(10):114-117.
[2]Hamilton D J,Dieterl R M,Felsot A S,et al.Regulatory Limits for Pesticide Residues in Water[J].Chemistry International--Newsmagazine for IUPAC,2003,25(6):21-23.
[3]叶宇飞,许秀琴,王立君,等.气相色谱法测定蔬菜水果中农药残留的基质效应研究[J].安徽农学通报,2015(5):114-116.
[4]高青珍.QuEChERS-色谱法快速测定蔬菜水果中的农药多残留[D].北京:中国农业科学院,2014.
[5]Diez C,Traag W A,Zommer P,et al.Comparison of An Acetonitrile Extraction/partitioning and“Dispersive Solid-phase Extraction”Method with Classical Multiresidue Methods for the Extraction of Herbicide Residues in Barley Samples[J].Journal of Chromatography A,2006,1131(1-2):11-23.
[6]黄雪松.大葱挥发油含量与化学成分的分析[J].食品与发酵工业,2004,30(10):114-117.
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