鼠尾草酸对大鼠大脑中动脉阻断引起的局灶性脑缺血的保护作用
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摘要
目的:研究鼠尾草酸预处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的保护作用。方法:将健康雄性SD大鼠随机分为7个组:假手术组、模型组、尼莫地平组、灯盏花素组、鼠尾草酸180 mg/kg鼠尾草酸90 mg/kg组、鼠尾草酸45 mg/kg组。各组大鼠连续灌胃给药7 d,末次给药后,采用改良线栓法制备大鼠大脑中动脉栓塞(Middle CerebralArtery Occlusion,MCAO)模型,观察鼠尾草酸预处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的影响。结果:鼠尾草酸180 mg/kg组能明显缩小脑梗死面积百分比,抑制血小板聚集,显著性降低纤维蛋白原(FIB)含量;鼠尾草酸90 mg/kg组能明显改善大鼠神经损伤症状,缩小脑梗死面积百分比,延长活化部分凝血活酶时间(Activated Partial ThromboplastinTime,ATPP),降低纤维蛋白原(Fibrinogen,FIB);鼠尾草酸45 mg/kg组能延长ATPP,明显降低纤维蛋白原(FIB)含量。此外,血常规结果显示,不同剂量鼠尾草酸预处理对MCAO大鼠的的各项生理指标起到一定的调节作用,差异具有统计学意义。结论:鼠尾草酸预处理对大鼠大脑中动脉阻断引起的局灶性脑缺血的具有良好的保护作用。
Objective Our aim is to study the role of carnosic acid preconditioning on focal cerebral ischemia caused by middle cerebral artery occlusion in rats. Methods Healthy male Sprague-Dawley rats were randomly divided into 7 groups: sham operation group,model group,nimodipine group,breviscapine group,carnosic acid 180 mg/kg group,carnosic acid 90 mg/kg group,and carnosic acid 45 mg/kg group. Rats in each group were administered with intragastric administration for 7 days continuously. After the last administration,the Middle Cerebral Artery Occlusion( MCAO) model of rats were prepared by modified suture method to observe the influence of focal cerebral ischemia caused by the treatment of rat middle cerebral artery occlusion. Results The percentage of cerebral infarction area were significantly reduced,the aggregation of platelets were inhibited,and fibrinogen( FIB) content dramatically was decreased in carnosic acid 180 mg/kg group. Then the symptoms of nerve damage in rats were remarkably improved,the percentage of cerebral infarction were reduced,the activated partial thromboplastin time( ATPP),and decrease fibrinogen( FIB) content were lengthened in carnosic acid 90 mg/kg group,compared with model group. Ultimately,the ATPP and fibrinogen( FIB) content of carnosic acid 45 mg/kg group were longer and lower than model group. In addition,blood routine test results showed that different doses of carnosic acid pretreatment had certain regulation effects on various physiological indexes of middle cerebral artery occlusion(MCAO) rats,and the difference was statistically significant among the groups. Conclusions Therefor,the pretreatment of carnosic acid had a good protective effect on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.
Objective Our aim is to study the role of carnosic acid preconditioning on focal cerebral ischemia caused by middle cerebral artery occlusion in rats. Methods Healthy male Sprague-Dawley rats were randomly divided into 7 groups: sham operation group,model group,nimodipine group,breviscapine group,carnosic acid 180 mg/kg group,carnosic acid 90 mg/kg group,and carnosic acid 45 mg/kg group. Rats in each group were administered with intragastric administration for 7 days continuously. After the last administration,the Middle Cerebral Artery Occlusion( MCAO) model of rats were prepared by modified suture method to observe the influence of focal cerebral ischemia caused by the treatment of rat middle cerebral artery occlusion. Results The percentage of cerebral infarction area were significantly reduced,the aggregation of platelets were inhibited,and fibrinogen( FIB) content dramatically was decreased in carnosic acid 180 mg/kg group. Then the symptoms of nerve damage in rats were remarkably improved,the percentage of cerebral infarction were reduced,the activated partial thromboplastin time( ATPP),and decrease fibrinogen( FIB) content were lengthened in carnosic acid 90 mg/kg group,compared with model group. Ultimately,the ATPP and fibrinogen( FIB) content of carnosic acid 45 mg/kg group were longer and lower than model group. In addition,blood routine test results showed that different doses of carnosic acid pretreatment had certain regulation effects on various physiological indexes of middle cerebral artery occlusion(MCAO) rats,and the difference was statistically significant among the groups. Conclusions Therefor,the pretreatment of carnosic acid had a good protective effect on focal cerebral ischemia caused by middle cerebral artery occlusion in rats.
引文
[1]范娅丹,韩江全,邓才洪.灯盏细辛与赤芍配伍对永久性脑缺血损伤大鼠的保护作用[J].中风与神经疾病,2016,33(10):894-896.
[2]谭燕萍.羟基红花黄色素-a与芍药苷对大鼠脑缺血再灌注损伤的保护作用及其对PI3K和AKT蛋白表达的影响[J].抗感染药学,2018(5):746-750.
[3]Li XX,Lu SF,Zhu BM,et al.Excitatory Amino Acids Toxicity and the Adjustment of Acupuncture Intervention[J].Ischemic Stroke,2016,41(2):180.
[4]Prabhakar O.Cerebroprotective effect of resveratrol through antioxidant and anti-inflammatory effects in diabetic rats[J].Naunyn-Schmiedeberg's archives of pharmacology,2013,386(8):705-710.
[5]Zhao J,Zhang X,Dong L,et al.Cinnamaldehyde inhibits inflammation and brain damage in a mouse model of permanent cerebral ischaemia[J].British journal of pharmacology,2015,172(20):5009-5023.
[6]张蔺珊,李娟娟,吴春云.脑缺血的损伤机制及相关信号通路的研究进展[J].神经解剖学杂志,2014,30(6):729-732.
[7]赵浩,李永宁,王任直,等.大鼠局灶性脑缺血模型的有效制备[J].中国实验动物学报,2009,17(6):432-436.
[8]展淑琴,高亚亚,张凌峰,等.大鼠大脑中动脉永久性缺血和缺血再灌注模型的比较[J].陕西医学杂志,2013,42(6):643-646.
[9]杨赞章,陈虹.线栓法制备大鼠局灶性脑缺血模型的改进与体会[J].时珍国医国药,2008,19(9):2189~2190.
[10]魏静,胡炜彦,张兰春,等.鼠尾草酸稳定性及对H2O2损伤神经元的保护作用[J].中药药理与临床,2016(2):43-46.
[11]Vázquez NM,Fiorilli G,Cáceres Guido PA,et al.Carnosic acid acts synergistically with gentamicin in killing methicillin-resistant Staphylococcus aureus clinical isolates[J].Phytomedicine:international journal of phytotherapy and phytopharmacology,2016,23(12):1337-1343.
[12]袁干军,李沛波,杨慧.迷迭香中鼠尾草酸的抗MR-SA活性研究[J].中国现代应用药学,2012(7):571-574.
[13]夏田娟,毕良武,赵振东,等.鼠尾草酸的抗氧化活性及抑菌活性研究[J].天然产物研究与开发,2015(1):35-40.
[14]Zhou X,Qi Y,Chen T.Long-term pre-treatment of antioxidant Ginkgo biloba extract EGb-761 attenuates cerebral-ischemia-induced neuronal damage in aged mice[J].Biomedicine&pharmacotherapy,2017(85):256-263.
[15]Zhang X,Chen Y,Cai G,et al.Carnosic acid induces apoptosis of hepatocellular carcinoma cells via ROS-mediated mitochondrial pathway[J].Chemico-biological interactions,2017(277):91-100.
[16]Maione F,Cantone V,Pace S,et al.Anti-inflammatory and analgesic activity of carnosol and carnosic acid in vivo and in vitro and in silico analysis of their target interactions[J].British journal of pharmacology,2017,174(11):1497-1508.
[17]胡炜彦,于浩飞,张荣平.鼠尾草酸对Aβ损伤海马神经元的保护作用研究[J].天然产物研究与开发,2014,26(4):490-493.
[18]段晓燕,张诤,潘勤,等.鼠尾草酸对肥胖性脂肪性肝炎大鼠血清生化指标和肝组织学变化的影响[J].实用肝脏病杂志,2012,15(4):295-298.
[19]刘振国.鼠尾草酸对四氯化碳诱导肝细胞损伤的作用研究[J].长沙:中南大学,2011:22.
[20]张兰春,于浩飞,程先睿,等.迷迭香脂溶性提取物对MCAO型大鼠脑缺血的保护作用[J].中药药理与临床,2014(6):109-111.
[21]包新杰,李雪元,左赋兴,等.稳定的大鼠大脑中动脉栓塞脑梗死模型的建立[J].中国实验动物学报,2016,24(4):395-398.
[22]王龙,金保哲,张新中.脑血流监测对大鼠脑缺血模型制备的评价作用[J].中国脑血管病杂志,2017,14(5).
[23]王玥琪,刘星,杨春壮,等.近十年来MCAO大鼠模型制备方法优缺点探讨分析[J].牡丹江医学院学报,2017,38(2):136-138.
[24]吴雅吟,陶静.电针对左侧MCAO大鼠皮质中shh及gli1的影响观察.亚太传统医药[J],2014,10(6):9-10.
[25]曹长春,金秋,谭东明.脑缺血与自由基损伤[J].北方药学,2014,11(3):109.
[26]庄礼源.脑梗死临床治疗研究进展[J].临床合理用药杂志,2017,10(9):173~175.
[27]冀思华,曹圣楷,曾光尧,等.迷迭香二萜酚类成分鼠尾草酸的研究进展[J].中南药学,2014(8):788-792.
[28]Loussouarn M,Krieger liszkay A,Svilar L,et al.Carnosic acid and carnosol,two major antioxidants of rosemary,act through different mechanisms[J].Plant physiology,2017,175(3):1183
[29]Yang Neng,Xia Zongling,Shao Naiyuan,et al.Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway[J].Scientific reports,2017,7(1):11036.
[2]谭燕萍.羟基红花黄色素-a与芍药苷对大鼠脑缺血再灌注损伤的保护作用及其对PI3K和AKT蛋白表达的影响[J].抗感染药学,2018(5):746-750.
[3]Li XX,Lu SF,Zhu BM,et al.Excitatory Amino Acids Toxicity and the Adjustment of Acupuncture Intervention[J].Ischemic Stroke,2016,41(2):180.
[4]Prabhakar O.Cerebroprotective effect of resveratrol through antioxidant and anti-inflammatory effects in diabetic rats[J].Naunyn-Schmiedeberg's archives of pharmacology,2013,386(8):705-710.
[5]Zhao J,Zhang X,Dong L,et al.Cinnamaldehyde inhibits inflammation and brain damage in a mouse model of permanent cerebral ischaemia[J].British journal of pharmacology,2015,172(20):5009-5023.
[6]张蔺珊,李娟娟,吴春云.脑缺血的损伤机制及相关信号通路的研究进展[J].神经解剖学杂志,2014,30(6):729-732.
[7]赵浩,李永宁,王任直,等.大鼠局灶性脑缺血模型的有效制备[J].中国实验动物学报,2009,17(6):432-436.
[8]展淑琴,高亚亚,张凌峰,等.大鼠大脑中动脉永久性缺血和缺血再灌注模型的比较[J].陕西医学杂志,2013,42(6):643-646.
[9]杨赞章,陈虹.线栓法制备大鼠局灶性脑缺血模型的改进与体会[J].时珍国医国药,2008,19(9):2189~2190.
[10]魏静,胡炜彦,张兰春,等.鼠尾草酸稳定性及对H2O2损伤神经元的保护作用[J].中药药理与临床,2016(2):43-46.
[11]Vázquez NM,Fiorilli G,Cáceres Guido PA,et al.Carnosic acid acts synergistically with gentamicin in killing methicillin-resistant Staphylococcus aureus clinical isolates[J].Phytomedicine:international journal of phytotherapy and phytopharmacology,2016,23(12):1337-1343.
[12]袁干军,李沛波,杨慧.迷迭香中鼠尾草酸的抗MR-SA活性研究[J].中国现代应用药学,2012(7):571-574.
[13]夏田娟,毕良武,赵振东,等.鼠尾草酸的抗氧化活性及抑菌活性研究[J].天然产物研究与开发,2015(1):35-40.
[14]Zhou X,Qi Y,Chen T.Long-term pre-treatment of antioxidant Ginkgo biloba extract EGb-761 attenuates cerebral-ischemia-induced neuronal damage in aged mice[J].Biomedicine&pharmacotherapy,2017(85):256-263.
[15]Zhang X,Chen Y,Cai G,et al.Carnosic acid induces apoptosis of hepatocellular carcinoma cells via ROS-mediated mitochondrial pathway[J].Chemico-biological interactions,2017(277):91-100.
[16]Maione F,Cantone V,Pace S,et al.Anti-inflammatory and analgesic activity of carnosol and carnosic acid in vivo and in vitro and in silico analysis of their target interactions[J].British journal of pharmacology,2017,174(11):1497-1508.
[17]胡炜彦,于浩飞,张荣平.鼠尾草酸对Aβ损伤海马神经元的保护作用研究[J].天然产物研究与开发,2014,26(4):490-493.
[18]段晓燕,张诤,潘勤,等.鼠尾草酸对肥胖性脂肪性肝炎大鼠血清生化指标和肝组织学变化的影响[J].实用肝脏病杂志,2012,15(4):295-298.
[19]刘振国.鼠尾草酸对四氯化碳诱导肝细胞损伤的作用研究[J].长沙:中南大学,2011:22.
[20]张兰春,于浩飞,程先睿,等.迷迭香脂溶性提取物对MCAO型大鼠脑缺血的保护作用[J].中药药理与临床,2014(6):109-111.
[21]包新杰,李雪元,左赋兴,等.稳定的大鼠大脑中动脉栓塞脑梗死模型的建立[J].中国实验动物学报,2016,24(4):395-398.
[22]王龙,金保哲,张新中.脑血流监测对大鼠脑缺血模型制备的评价作用[J].中国脑血管病杂志,2017,14(5).
[23]王玥琪,刘星,杨春壮,等.近十年来MCAO大鼠模型制备方法优缺点探讨分析[J].牡丹江医学院学报,2017,38(2):136-138.
[24]吴雅吟,陶静.电针对左侧MCAO大鼠皮质中shh及gli1的影响观察.亚太传统医药[J],2014,10(6):9-10.
[25]曹长春,金秋,谭东明.脑缺血与自由基损伤[J].北方药学,2014,11(3):109.
[26]庄礼源.脑梗死临床治疗研究进展[J].临床合理用药杂志,2017,10(9):173~175.
[27]冀思华,曹圣楷,曾光尧,等.迷迭香二萜酚类成分鼠尾草酸的研究进展[J].中南药学,2014(8):788-792.
[28]Loussouarn M,Krieger liszkay A,Svilar L,et al.Carnosic acid and carnosol,two major antioxidants of rosemary,act through different mechanisms[J].Plant physiology,2017,175(3):1183
[29]Yang Neng,Xia Zongling,Shao Naiyuan,et al.Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway[J].Scientific reports,2017,7(1):11036.