木薯器官及其腐解物水浸液对橡胶树白根病病菌的化感作用
|
摘要
本文采用菌丝生长速率法研究木薯根、茎、叶及其腐解物水浸液对橡胶树白根病病菌的化感效果。结果表明:(1)根水浸液在处理后2～5 d均呈现出显著的化感促进效果,不同浓度之间的化感效果差异不明显;叶水浸液在处理后2 d和5 d均有显著的化感促进效果,处理后3 d和4 d,仅10和100 mg/mL的叶水浸液呈现显著的化感促进效果;茎水浸液在处理后2～3 d的化感促进效果均不明显,但100 mg/mL的茎水浸液在处理后4 d和1000 mg/mL茎水浸液在处理后5 d呈现显著的化感促进效果。(2)根腐解物水浸液化感抑制效果随处理浓度的增加而增加,浓度增至200 mg/mL时差异达到显著水平;叶腐解物水浸液化感抑制作用随浓度的增加而逐渐下降,当浓度增至200 mg/mL时化感效果不明显;茎腐解物水浸液均有显著的化感抑制作用。
The allelopathic effect of water extracts from the root, stem or leaf of cassava and the decomposing products on the Rigidoporus lignosus(Klotzsch) Imaz. of rubber tree at three concentrations and different days after treatment were studied using the mycelial growth rate method. The water extracts from the root of cassava could significantly promote the growth of Rigidoporus lignosus(Klotzsch) Imaz. between the 2–5th day after treatment, but there was no significant difference among the three concentrations. The promotion effect of the three concentrations extracts from the leaf was significant at the 2th or 5th day after treatment, while the promotion effect to the 10 mg/mL and 100 mg/mL extracts was significant at the 3th day or 4th day. The allelopathic promotion effect of the extracts from the stem was not obvious at the 2th or 3th day, while there was a significantly positive effect of the 100 mg/mL extracts at the 4th and of 1000 mg/mL extracts at the 5th day. The allelopathic inhibition effect of the extracts from the root decomposing product increased gradually with the concentration increased. The effect was significant when the concentration increased to 200 mg/mL. With the concentration increased, the inhibition effect of the extracts from the leaf decomposing product decreased gradually. The effect was no significant when the concentration increased to 200 mg/mL. The inhibition effect of the extracts from the stem decomposing product was significant.
The allelopathic effect of water extracts from the root, stem or leaf of cassava and the decomposing products on the Rigidoporus lignosus(Klotzsch) Imaz. of rubber tree at three concentrations and different days after treatment were studied using the mycelial growth rate method. The water extracts from the root of cassava could significantly promote the growth of Rigidoporus lignosus(Klotzsch) Imaz. between the 2–5th day after treatment, but there was no significant difference among the three concentrations. The promotion effect of the three concentrations extracts from the leaf was significant at the 2th or 5th day after treatment, while the promotion effect to the 10 mg/mL and 100 mg/mL extracts was significant at the 3th day or 4th day. The allelopathic promotion effect of the extracts from the stem was not obvious at the 2th or 3th day, while there was a significantly positive effect of the 100 mg/mL extracts at the 4th and of 1000 mg/mL extracts at the 5th day. The allelopathic inhibition effect of the extracts from the root decomposing product increased gradually with the concentration increased. The effect was significant when the concentration increased to 200 mg/mL. With the concentration increased, the inhibition effect of the extracts from the leaf decomposing product decreased gradually. The effect was no significant when the concentration increased to 200 mg/mL. The inhibition effect of the extracts from the stem decomposing product was significant.
引文
[1]鞠岩峰,张剑,吴润,等.天然橡胶种植现状及市场需求预测分析[J].林业资源管理,2014(1):152-157.
[2]张慧坚,方佳,孙好勤.我国热带作物产业发展现状及趋势分析[J].江西农业学报,2010,22(5):173-179.
[3]蔡吉苗,陈瑶,潘羡心,等.海南橡胶树棒孢霉落叶病病情调查与病原鉴定[J].热带农业科学,2008,28(5):1-7,10.
[4]杨国锋.加蓬的胶粮间作系统[J].世界热带农业信息,2000(6):1-2.
[5]邬若兰,尹维,廖晓兰.植物化感作用防治植物病害研究进展[J].农业灾害研究,2013,3(7):42-44,62.
[6]宋文超,贾俊英,云兴福.西芹鲜根浸提液对黄瓜枯萎病菌化感作用机理的研究-鲜根浸提物处理后黄瓜枯萎病菌形态及孢子生长的变化[J].内蒙古农业大学学报,2010,31(3):100-105.
[7]杨玉锋,姚战军,李保利.西芹根水浸提液对西瓜枯萎病菌的化感作用[J].江苏农业科学,2011,39(6):224-225.
[8]刘顺湖,吕秀红.金乡大蒜秸秆浸提液对黄瓜灰霉病菌抑制效果的研究[J].济宁学院学报,2014,35(6):5-10.
[9]齐永志.根系化感物质与病原菌在草莓连作障碍中的协同作用研究[D].保定:河北农业大学,2008.
[10]陈建新,刘家运,刘翠珍,等.不同处理方法对木薯氢氰酸含量的影响[J].广东畜牧兽医科技,1992(4):13-14.
[11]何翠薇,陈玉萍,覃洁萍,等.木薯茎杆及叶化学成分初步研究[J].时珍国医国药,2011,22(4):908-909.
[12]李珊珊,戴好富,赵友兴,等.海南产木薯茎化学成分研究[J].热带亚热带植物学报,2012,20(2):197-200.
[13]柳红娟,黄洁,刘子凡,等.木薯器官浸提液对香蕉尖孢镰刀菌的抑菌作用[J].云南农业大学学报(自然科学版),2016,31(3):556-561.
[14]刘培培,刘子凡,刘乔颖,等.木薯器官及其混合物水浸提液对橡胶树棒孢霉落叶病病菌的化感作用[J].热带作物学报,2018,39(2):338-342.
[15]Tran Van Canh,张开明.不同杀菌剂防治橡胶树白根病的研究[J].热带作物译丛,1984(4):19-22.
[16]秦立金,曹巨峰,韩伟秋,等.黄瓜和西芹间作对黄瓜生长及枯萎病发生的影响[J].中国生态农业学报,2018,26(5):684-692.
[17]王桂清,马迪.室内药效试验方法的比较[J].江苏农业科学,2017,45(19):175-178.
[18]鲁海菊,张晓永,江涛,等.抗枇杷根腐病病菌的枇杷内生木霉P3.9菌株生物学特性[J].江苏农业科学,2018,46(2):53-57.
[19]韩旭.大蒜秸秆腐解物化感作用研究及化感物质鉴定[D].杨凌:西北农林科技大学,2013.
[20]田给林.连作草莓土壤酚酸类物质的化感作用及其生物调控研究[D].北京:中国农业大学,2015.
[21]孔垂华,胡飞,王朋.植物化感(相生相克)作用[M].北京:高等教育出版社,2016.
[22]邬彩霞,刘苏娇,赵国琦.黄花草木樨水浸提液中潜在化感物质的分离、鉴定[J].草业学报,2014,23(5):184-192.
[23]Wallenstein M D,Hess A M,Lewis M R,et al.Decomposition of aspen leaf litter results in unique metabolomes when decomposed under different tree species[J].Soil Biology&Biochemistry,2010,42:484-490.
[24]程莹,白寿发,庄敬华,等.甜瓜残茬腐解物对镰孢枯萎病的助长作用[J].中国农学通报,2011,27(8):217-221.
[25]Blagbrough I S,Bayoumi S A L,Rowanet M G,et al.Cassava:An appraisal of its phytochemistry and its biotechnological prospects[J].Phytochemistry,2010,71(17-18):1940-1951.
[26]谭秀梅,王华田,孔令刚,等.杨树人工林连作土壤中酚酸积累规律及对土壤微生物的影响[J].山东大学学报(理学版),2008,43(1):14-19.
[27]刘建国.新疆棉花长期连作的土壤环境效应及其化感作用的研究[D].南京:南京农业大学,2008.
[28]Orhan D D,Ozcelik B,Ozgen S,et al.Antibacterial,antifungal,and antiviral activities of some flavonoids[J].Microbiologial Research,2010,165(6):496-504.
[29]吴会芹,董林林,王倩.玉米、小麦秸秆水浸提液对蔬菜种子的化感作用[J].华北农学报,2009,24(12):140-143.
[30]Dong S Q,Ma Y Q,Wu H W,et al.Stimulatory effects of wheat(Triticum aestivum L.)on seed germination of Orobanche minor Sm[J].Allelopathy Journal,2012,30(2):247-258.
[31]韩燕,云兴福.西芹根水浸提液对黄瓜枯萎病菌的化感作用[J].内蒙古农业大学学报(自然科学版),2007,28(4):112-115.
[2]张慧坚,方佳,孙好勤.我国热带作物产业发展现状及趋势分析[J].江西农业学报,2010,22(5):173-179.
[3]蔡吉苗,陈瑶,潘羡心,等.海南橡胶树棒孢霉落叶病病情调查与病原鉴定[J].热带农业科学,2008,28(5):1-7,10.
[4]杨国锋.加蓬的胶粮间作系统[J].世界热带农业信息,2000(6):1-2.
[5]邬若兰,尹维,廖晓兰.植物化感作用防治植物病害研究进展[J].农业灾害研究,2013,3(7):42-44,62.
[6]宋文超,贾俊英,云兴福.西芹鲜根浸提液对黄瓜枯萎病菌化感作用机理的研究-鲜根浸提物处理后黄瓜枯萎病菌形态及孢子生长的变化[J].内蒙古农业大学学报,2010,31(3):100-105.
[7]杨玉锋,姚战军,李保利.西芹根水浸提液对西瓜枯萎病菌的化感作用[J].江苏农业科学,2011,39(6):224-225.
[8]刘顺湖,吕秀红.金乡大蒜秸秆浸提液对黄瓜灰霉病菌抑制效果的研究[J].济宁学院学报,2014,35(6):5-10.
[9]齐永志.根系化感物质与病原菌在草莓连作障碍中的协同作用研究[D].保定:河北农业大学,2008.
[10]陈建新,刘家运,刘翠珍,等.不同处理方法对木薯氢氰酸含量的影响[J].广东畜牧兽医科技,1992(4):13-14.
[11]何翠薇,陈玉萍,覃洁萍,等.木薯茎杆及叶化学成分初步研究[J].时珍国医国药,2011,22(4):908-909.
[12]李珊珊,戴好富,赵友兴,等.海南产木薯茎化学成分研究[J].热带亚热带植物学报,2012,20(2):197-200.
[13]柳红娟,黄洁,刘子凡,等.木薯器官浸提液对香蕉尖孢镰刀菌的抑菌作用[J].云南农业大学学报(自然科学版),2016,31(3):556-561.
[14]刘培培,刘子凡,刘乔颖,等.木薯器官及其混合物水浸提液对橡胶树棒孢霉落叶病病菌的化感作用[J].热带作物学报,2018,39(2):338-342.
[15]Tran Van Canh,张开明.不同杀菌剂防治橡胶树白根病的研究[J].热带作物译丛,1984(4):19-22.
[16]秦立金,曹巨峰,韩伟秋,等.黄瓜和西芹间作对黄瓜生长及枯萎病发生的影响[J].中国生态农业学报,2018,26(5):684-692.
[17]王桂清,马迪.室内药效试验方法的比较[J].江苏农业科学,2017,45(19):175-178.
[18]鲁海菊,张晓永,江涛,等.抗枇杷根腐病病菌的枇杷内生木霉P3.9菌株生物学特性[J].江苏农业科学,2018,46(2):53-57.
[19]韩旭.大蒜秸秆腐解物化感作用研究及化感物质鉴定[D].杨凌:西北农林科技大学,2013.
[20]田给林.连作草莓土壤酚酸类物质的化感作用及其生物调控研究[D].北京:中国农业大学,2015.
[21]孔垂华,胡飞,王朋.植物化感(相生相克)作用[M].北京:高等教育出版社,2016.
[22]邬彩霞,刘苏娇,赵国琦.黄花草木樨水浸提液中潜在化感物质的分离、鉴定[J].草业学报,2014,23(5):184-192.
[23]Wallenstein M D,Hess A M,Lewis M R,et al.Decomposition of aspen leaf litter results in unique metabolomes when decomposed under different tree species[J].Soil Biology&Biochemistry,2010,42:484-490.
[24]程莹,白寿发,庄敬华,等.甜瓜残茬腐解物对镰孢枯萎病的助长作用[J].中国农学通报,2011,27(8):217-221.
[25]Blagbrough I S,Bayoumi S A L,Rowanet M G,et al.Cassava:An appraisal of its phytochemistry and its biotechnological prospects[J].Phytochemistry,2010,71(17-18):1940-1951.
[26]谭秀梅,王华田,孔令刚,等.杨树人工林连作土壤中酚酸积累规律及对土壤微生物的影响[J].山东大学学报(理学版),2008,43(1):14-19.
[27]刘建国.新疆棉花长期连作的土壤环境效应及其化感作用的研究[D].南京:南京农业大学,2008.
[28]Orhan D D,Ozcelik B,Ozgen S,et al.Antibacterial,antifungal,and antiviral activities of some flavonoids[J].Microbiologial Research,2010,165(6):496-504.
[29]吴会芹,董林林,王倩.玉米、小麦秸秆水浸提液对蔬菜种子的化感作用[J].华北农学报,2009,24(12):140-143.
[30]Dong S Q,Ma Y Q,Wu H W,et al.Stimulatory effects of wheat(Triticum aestivum L.)on seed germination of Orobanche minor Sm[J].Allelopathy Journal,2012,30(2):247-258.
[31]韩燕,云兴福.西芹根水浸提液对黄瓜枯萎病菌的化感作用[J].内蒙古农业大学学报(自然科学版),2007,28(4):112-115.