江浙地区秀珍菇双翅目害虫鉴定及防治
|
摘要
【目的】明确江浙地区秀珍菇双翅目害虫的种类、发生规律及其生物防治方法,为秀珍菇双翅目害虫防控提供科学依据。【方法】在江苏灌南、高淳和浙江淳安、江山的7个秀珍菇栽培基地采集双翅目害虫虫样,采用形态学特征和分子生物学方法对虫样进行分类鉴定;采用黄板诱虫法监测秀珍菇出菇棚害虫全年动态消长规律,绘制双翅目害虫消长动态曲线;在秀珍菇出菇期,采用喷雾法测定1%印楝素水分散粒剂1000倍稀释液和1200 ITU/mg苏云金芽孢杆菌以色列变种(Bti)可湿性粉剂500倍稀释液对双翅目害虫的防治效果。【结果】在江浙地区7个秀珍菇栽培基地采集虫样70份,通过形态学和分子生物学鉴定发现当地秀珍菇栽培中受到4科[眼蕈蚊科(Sciaridae)、蚤蝇科(Phoridae)、果蝇科(Drosophilidae)和瘿蚊科(Cecidomyiidae)]5属[迟眼蕈蚊属(Bradysia impatiens)、厉眼蕈蚊属(Lycoriella ingenua)、异蚤蝇属(Megaselia tamilncduensis)、果蝇属(Drosophila glabriapex)和瘿蚊科未知属]双翅目虫害的危害,其中迟眼蕈蚊属的异迟眼蕈蚊发生范围最广、样本数最多;黄板监测结果显示,江浙地区秀珍菇双翅目害虫全年在5—7月和9—10月出现两个高峰;对害虫的防治试验结果表明,印楝素和Bti对秀珍菇上的双翅目害虫均有明显的杀虫活性,第2次用药15 d后黄板上的平均成虫数分别只有89.73和101.27只/板,明显少于对照虫数(281.23只/板),印楝素防治效果略优于Bti,但差异不显著(P>0.05)。【结论】迟眼蕈蚊属的异迟眼蕈蚊是江浙地区秀珍菇栽培中害虫种类的优势种群;在江浙地区秀珍菇出菇棚中双翅目害虫全年有两个发生高峰,分别为5—7月和9—10月;印楝素和Bti两种生物药剂对秀珍菇上的双翅目害虫均有明显的杀虫效果,可作为防治秀珍菇双翅目害虫的药剂在生产上推广使用。
【Objective】In order to provide scientific basis for the control of diptera pests on Pleurotus pulmonarius,the species,regularity of outbreak and biological control method of diptera pests on P. pulmonarius in the regions of Jiangsu and Zhejiang were studied.【Method】Diptera pests samples were collected in seven cultivation bases of P. pulmonarius in Guannan and Gaochun of Jiangsu as well as Chun'an and Jiangshan of Zhejiang. The collected pest samples were classified and identified by using morphological feature and molecular biological method. The dynamic curve of diptera pests was drawn according to annual dynamic regularity of pests in P. pulmonarius growth shed with yellow board insect trapping method. The control efficiency of 1% azadirachtin water dispersible granule(WDG)(1000-time diluent)and 1200ITU/mg Bacillus thuringiensis var. tsraelensis(Bti)wettable powder(WP)(500-time diluent)against diptera pests were determined by using spray method during P. pulmonarius growth period.【Result】Seventy pest samples were collected from seven cultivation bases of P. pulmonarius in four places of Jiangsu and Zhejiang. And 70 pest samples were identified by using morphology and molecular biology. The pest sample identification results showed that the cultivation of P. pulmonarius in Jiangsu and Zhejiang was damaged by four families of diptera pests containing Sciaridae,Phoridae,Drosophilidae and Cecidomyiidae and five genera including Bradysia impatiens,Lycoriella ingenua,Megaselia tamilncduensis,Drosophila glabriapex,and an unknown genus of Cecidomyiidae. The occurance area of Bradysia difformis of B. impatiens was the widest with the largest number of samples. The monitoring results of yellow board showed that there were two outbreak peaks of diptera pests on P. pulmonarius in the regions of Jiangsu and Zhejiang all the year round,May to July and September to October. The results of pest control test showed that both azadirachtin and Bti exhibited obvious insecticidal activity against diptera pests on P. pulmonarius. 15 d after second application of azadirachtin and Bti,the average adult quantities of diptera pests on each yellow board were 89.73 and 101.27 respectively. These two numbers were obviously lower than the number of control(281.23 pesst/board). In addition,the control effect of azadirachtin was better than that of Bti,but the difference was not significant(P>0.05).【Conclusion】B. difformis of B. impatiens is the dominant population of pest species in P. pulmonarius cultivation in the regions of Jiangsu and Zhejiang. Besides,there are two outbreak peaks of diptera pests in P. pulmonarius growth shed in the regions of Jiangsu and Zhejiang all the year round,May to July and September to October. Moreover,two biological pesticides,Bti and azadirachtin,have in secticidal efficiency against diptera pests on P. pulmonarius,so they can be used as pesticides for the control of diptera pests on P. pulmonarius and are popularized and applied in production.
【Objective】In order to provide scientific basis for the control of diptera pests on Pleurotus pulmonarius,the species,regularity of outbreak and biological control method of diptera pests on P. pulmonarius in the regions of Jiangsu and Zhejiang were studied.【Method】Diptera pests samples were collected in seven cultivation bases of P. pulmonarius in Guannan and Gaochun of Jiangsu as well as Chun'an and Jiangshan of Zhejiang. The collected pest samples were classified and identified by using morphological feature and molecular biological method. The dynamic curve of diptera pests was drawn according to annual dynamic regularity of pests in P. pulmonarius growth shed with yellow board insect trapping method. The control efficiency of 1% azadirachtin water dispersible granule(WDG)(1000-time diluent)and 1200ITU/mg Bacillus thuringiensis var. tsraelensis(Bti)wettable powder(WP)(500-time diluent)against diptera pests were determined by using spray method during P. pulmonarius growth period.【Result】Seventy pest samples were collected from seven cultivation bases of P. pulmonarius in four places of Jiangsu and Zhejiang. And 70 pest samples were identified by using morphology and molecular biology. The pest sample identification results showed that the cultivation of P. pulmonarius in Jiangsu and Zhejiang was damaged by four families of diptera pests containing Sciaridae,Phoridae,Drosophilidae and Cecidomyiidae and five genera including Bradysia impatiens,Lycoriella ingenua,Megaselia tamilncduensis,Drosophila glabriapex,and an unknown genus of Cecidomyiidae. The occurance area of Bradysia difformis of B. impatiens was the widest with the largest number of samples. The monitoring results of yellow board showed that there were two outbreak peaks of diptera pests on P. pulmonarius in the regions of Jiangsu and Zhejiang all the year round,May to July and September to October. The results of pest control test showed that both azadirachtin and Bti exhibited obvious insecticidal activity against diptera pests on P. pulmonarius. 15 d after second application of azadirachtin and Bti,the average adult quantities of diptera pests on each yellow board were 89.73 and 101.27 respectively. These two numbers were obviously lower than the number of control(281.23 pesst/board). In addition,the control effect of azadirachtin was better than that of Bti,but the difference was not significant(P>0.05).【Conclusion】B. difformis of B. impatiens is the dominant population of pest species in P. pulmonarius cultivation in the regions of Jiangsu and Zhejiang. Besides,there are two outbreak peaks of diptera pests in P. pulmonarius growth shed in the regions of Jiangsu and Zhejiang all the year round,May to July and September to October. Moreover,two biological pesticides,Bti and azadirachtin,have in secticidal efficiency against diptera pests on P. pulmonarius,so they can be used as pesticides for the control of diptera pests on P. pulmonarius and are popularized and applied in production.
引文
蔡邦华.2015.昆虫分类学[M].修订版.北京:化学工业出版社:748-882.[Cai B H.2015.Entomological Taxonomy[M].Revised Edition.Beijing:Chemical Industry Press:748-882.]
陈躬国,林原,刘新锐,赵光辉,陈剑.2017.秀珍菇遗传育种研究进展[J].热带作物学报,38(7):1377-1381.[Chen GG,Lin Y,Liu X R,Zhao G H,Chen J.2017.Recent advances in genetics and breeding of Pleurotus pulmonarius[J].Chinese Journal of Tropical Crops,38(7):1377-1381.]
韩文贺.2016.4种农药对秀珍菇虫害防治及产品安全性影响的研究[J].食用菌,(4):48-50.[Han W H.2016.The study on the pest control of Pleurotus geesteranus and the effect of product safety on four pesticides[J].Edible Fungi,(4):48-50.]
姜婷婷,覃培升,黎金锋,刘斌,李子玲,谭梦超,王瑾,吴宗华,梁锟,罗燕卿.2016.广西南宁市平菇蚊、蝇类害虫常见种鉴定及消长规律研究[J].南方农业学报,47(1):67-73.[Jiang T T,Qin P S,Li J F,Liu B,Li Z L,Tan M C,Wang J,Wu Z H,Liang K,Luo Y Q.2016.Common species identification and growth-declinelaw of mosquitoes and flies damaging Pleurotus ostreatus in Nanning,Guangxi[J].Journal of Southern Agriculture,47(1):67-73.]
孔繁建,张智,谢爱婷,吴晓晨,师迎春,崔丽娥.2016.黄板不同悬挂位置和高度对平菇菇房眼蕈蚊诱杀效果的影响[J].中国植保导刊,36(4):34-36.[Kong F J,Zhang Z,Xie A T,Wu X C,Shi Y C,Cui L E.2016.The trapping effects of yellow boards hanged different location and height against sciaridae in Pleurotus ostreatus mushroom house[J].China Plant Protection,36(4):34-36.]
兰玉菲,蔚承祥,于清伟,崔晓.2016.平菇反季节栽培中双翅目害虫防治的药剂筛选及综合防控技术[J].中国食用菌,35(5):48-50.[Lan Y F,Yu C X,Yu Q W,Cui X.2016.Pesticides screening and integrated pest control technology in anti-season cultivation of Pleurotus ostreatus[J].Edible Fungi of China,35(5):48-50.]
雷雨霞,李红玉,李蝶,刘斌,李子玲.2016.四种杀虫剂对异迟眼蕈蚊的防治效果[J].北方园艺,(8):108-111.[Lei Y X,Li H Y,Li D,Liu B,Li Z L.2016.Control efficiency of four insecticides to Bradysia difformis[J].Northern Horticulture,(8):108-111.]
李光强.2009.食用菌害虫优势种生物学、种群动态和群落特征的研究[D].泰安:山东农业大学.[Li G Q.2009.Researches on bionomics and population dynamics of the dorminant edible fungus insect pests and characteristics of the insect communities on main edible fungi[D].Tai’an:Shandong Agricultural University.]
李建波,何璞.2017.我国食用菌双翅目害虫种类和防治研究现状[J].中国植保导刊,37(11):19-25.[Li J B,He P.2017.Research progress on diptera pest species of mushroom and its controlling in China[J].China Plant Protection,37(11):19-25.]
刘跃钧,王思贵,郑文彪,刘爱英,傅双凤.2003.秀珍菇生物学特性及高产栽培技术[J].食用菌,25(4):37-38.[Liu Y J,Wang S G,Zheng W B,Liu A Y,Fu S F.2003.The biological characteristics and high-yield culture technique of Pleurotus pulmonarius[J].Edible Fungi,25(4):37-38.]
罗佳,庄秋林.2007.福建食用菌双翅目害虫的种类、为害及防治[J].福建农林大学学报(自然科学版),36(3):237-240.[Luo J,Zhuang Q L.2007.The species,damage and control of Diptera pests in edible fungi in Fujian Province[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),36(3):237-240.]
马林,林金盛,曲绍轩,李辉平,侯立娟,蒋宁,宋金俤.2017.三种杀虫剂对从南京食用菌大棚随机捕获的Bradysia的防治效果[J].食用菌学报,24(3):87-90.[Ma L,Lin JS,Qu S X,Li H P,Hou L J,Jiang N,Song J D.2017.Efficacy of thiamethoxam,pymetrozine and acetamipridin controlling Bradysia under laboratory conditions and in a Pleurotus ostreatus cultivation facility[J].Acta Edulis Fungi,24(3):87-90.]
马林,曲绍轩,林金盛,宋金俤,李辉平,侯立娟,蒋宁.2015.食用菌迟眼蕈蚊生防菌苏云金芽孢杆菌的筛选及毒力测定[J].食用菌,(6):53-55.[Ma L,Qu S X,Lin J S,Song J D,Li H P,Hou L J,Jiang N.2015.Screening and toxicity test of biocontrol bacterium Bacillus thuringiensis against mushroom Bradysia[J].Edible Fungi,(6):53-55.]
曲绍轩,马林,华秀红,宋金俤.2010.古田山多菌蚊物理防治方法筛选试验[J].食用菌,(1):59-60.[Qu S X,Ma L,Hua X H,Song J D.2010.Screening tests of physical control methods against Docosia gutiuushana[J].Edible Fungi,(1):59-60.]
曲绍轩,马林,宋金俤,李辉平,华秀红.2012.食用菌眼蕈蚊的分子鉴定及2种植物源农药的室内毒力测定[J].江苏农业科学,40(9):123-125.[Qu S X,Ma L,Song J D,Li H P,Hua X H.2012.Molecular identification of mushroom sciarid flies and indoor toxicity tests of two botanical pesticide[J].Jiangsu Agricultural Sciences,40(9):123-125.]
沈登荣,张宏瑞,李正跃,和绍禹,张陶.2009.云南食用菌眼蕈蚊分类及优势种分析[J].昆虫学报,52(8):934-940.[Shen D R,Zhang H R,Li Z Y,He S Y,Zhang T.2009.Taxonomy and dominance analysis of sciarid fly species(Diptera:Sciaridae)on edible fungi in Yunnan[J].Acta Entomologica Sinica,52(8):934-940.]
沈迎春,张怡,钱忠海,刘琴,王林贵.2017.生物农药防治双孢蘑菇菇蚊菇蝇效果初探--以苏云金杆菌以色列亚种为例[J].农药科学与管理,38(11):47-51.[Shen Y C,Zhang Y,Qian Z H,Liu Q,Wang L G.2017.Preliminary exploration of the effect on biopesticide,such as Bacillus thuringiensis subsp.Israelensis,against mosquitoes and flies in Agaricus bisporus cultivation[J].Pesticide Science and Administration,38(11):47-51.]
师迎春,杨秀芬,张涛,胡彬,丁守付,戴宇婷.2014.苏云金芽孢杆菌制剂对双孢蘑菇栽培房眼蕈蚊的控制作用[J].食用菌学报,21(4):76-80.[Shi Y C,Yang X F,Zhang T,Hu B,Ding S F,Dai Y T.2014.Effectiveness of Bacillus thuringiensis microbial agents in controlling sciarid fly infestation in Agaricus bisporus cultivation rooms[J].Acta Edulis Fungi,21(4):76-80.]
宋爽,刘宇,王兰青,王守现,许峰,黄晨阳.2015.三种生物源农药对平菇双翅目害虫的药效试验[J].食用菌,(5):57-58.[Song S,Liu Y,Wang L Q,Wang S X,Xu F,Huang C Y.2015.Efficacy experiment of three biopesticides against diptera pest in Pleurotus ostreatus cultivation[J].Edible Fungi,(5):57-58.]
王丹丹.2012.平菇昆虫种群动态、群落特征和平菇厉眼蕈蚊内禀生物学特性研究[D].泰安:山东农业大学.[Wang D D.2012.Researches on insect population dynamics and community characteristics on Pleurotus ostreatus and intrinsic ecological characteristics of Lycoriella pleuroti Yang et Zhang[D].Tai’an:Shandong Agricultural University.]
王瑾.2013.南宁市平菇菇蚊菇蝇种类调查及物理防治技术研究[D].南宁:广西大学.[Wang J.2013.Species investigation and study of physical control of mosquitoes and flies on Pleurotus ostreatus in Nanning[D].Nanning:Guangxi University.]
温志强,边广.2011.秀珍菇菇蚊菇蝇的消长规律与物理防治技术[J].热带作物学报,32(10):1931-1934.[Wen ZQ,Bian G.2011.Preliminary study of variation and physical control techniques on mushroom flies of Pleurotus geesteranus Singer[J].Chinese Journal of Tropical Crops,32(10):1931-1934.]
徐汉虹,赖多,张志祥.2017.植物源农药印楝素的研究与应用[J].华南农业大学学报,38(4):1-11.[Xu H H,Lai D,Zhang Z X.2017.Research and application of botanical pesticide azadirachtin[J].Journal of South China A-gricultural University,38(4):1-11.]
张超逸.2017.沈阳地区平菇主要害虫种类调查和发生规律研究[D].沈阳:沈阳大学.[Zhang C Y.2017.The main pest species and dynamic regularity of oyster mushroom in Shenyang area[D].Shenyang:Shenyang University.]
张孔金,黎志银,谢宝贵,黄志龙.2012.不同农药对秀珍菇生长及产品安全性影响的研究[J].食药用菌,20(5):292-294.[Zhang K J,Li Z Y,Xie B G,Huang Z L.2012.The study on the effect of Pleurotus geesteranus growth and product safety on different pesticides[J].Edible and Medicinal Mushrooms,20(5):292-294.]
张晓云,沈登荣,李正跃,张陶,张宏瑞.2007.食用菌双翅目害虫的采集和玻片标本制作方法[J].中国食用菌,26(5):56-57.[Zhang X Y,Shen D R,Li Z Y,Zhang T,Zhang H R.2007.Collection and slide preparation methods of dipterous pests on edible mushrooms[J].Edible Fungi of China,26(5):56-57.]
赵晓娜,廖慧东,罗佳,吴梅香,范青海.2011.杀虫剂对黑腹果蝇毒力测定及对香菇和秀珍菇菌丝生长的影响[J].江西农业大学学报,33(2):283-286.[Zhao X N,Liao H D,Luo J,Wu M X,Fan Q H.2011.Toxicities of 4 insecticides to Drosophila melanogaster and their effcects on growth of Lentinus edodes and Pleurotu sgeesteranus[J].Acta Agriculturae Universitatis Jiangxiensis,33(2):283-286.]
Ross A,Willson V L.2017.Basic and Advanced Statistical Tests[M].Rotterdam:Sense Publishers:21-24.
Thonghua T,Thonghua J.2015.The efficacy of insecticidal control in Pomelo fruit fly IPM program in Nakhon Si Thammarat Province,Thailand[J].International Journal of Agricultural Technology,11(2):391-397.
陈躬国,林原,刘新锐,赵光辉,陈剑.2017.秀珍菇遗传育种研究进展[J].热带作物学报,38(7):1377-1381.[Chen GG,Lin Y,Liu X R,Zhao G H,Chen J.2017.Recent advances in genetics and breeding of Pleurotus pulmonarius[J].Chinese Journal of Tropical Crops,38(7):1377-1381.]
韩文贺.2016.4种农药对秀珍菇虫害防治及产品安全性影响的研究[J].食用菌,(4):48-50.[Han W H.2016.The study on the pest control of Pleurotus geesteranus and the effect of product safety on four pesticides[J].Edible Fungi,(4):48-50.]
姜婷婷,覃培升,黎金锋,刘斌,李子玲,谭梦超,王瑾,吴宗华,梁锟,罗燕卿.2016.广西南宁市平菇蚊、蝇类害虫常见种鉴定及消长规律研究[J].南方农业学报,47(1):67-73.[Jiang T T,Qin P S,Li J F,Liu B,Li Z L,Tan M C,Wang J,Wu Z H,Liang K,Luo Y Q.2016.Common species identification and growth-declinelaw of mosquitoes and flies damaging Pleurotus ostreatus in Nanning,Guangxi[J].Journal of Southern Agriculture,47(1):67-73.]
孔繁建,张智,谢爱婷,吴晓晨,师迎春,崔丽娥.2016.黄板不同悬挂位置和高度对平菇菇房眼蕈蚊诱杀效果的影响[J].中国植保导刊,36(4):34-36.[Kong F J,Zhang Z,Xie A T,Wu X C,Shi Y C,Cui L E.2016.The trapping effects of yellow boards hanged different location and height against sciaridae in Pleurotus ostreatus mushroom house[J].China Plant Protection,36(4):34-36.]
兰玉菲,蔚承祥,于清伟,崔晓.2016.平菇反季节栽培中双翅目害虫防治的药剂筛选及综合防控技术[J].中国食用菌,35(5):48-50.[Lan Y F,Yu C X,Yu Q W,Cui X.2016.Pesticides screening and integrated pest control technology in anti-season cultivation of Pleurotus ostreatus[J].Edible Fungi of China,35(5):48-50.]
雷雨霞,李红玉,李蝶,刘斌,李子玲.2016.四种杀虫剂对异迟眼蕈蚊的防治效果[J].北方园艺,(8):108-111.[Lei Y X,Li H Y,Li D,Liu B,Li Z L.2016.Control efficiency of four insecticides to Bradysia difformis[J].Northern Horticulture,(8):108-111.]
李光强.2009.食用菌害虫优势种生物学、种群动态和群落特征的研究[D].泰安:山东农业大学.[Li G Q.2009.Researches on bionomics and population dynamics of the dorminant edible fungus insect pests and characteristics of the insect communities on main edible fungi[D].Tai’an:Shandong Agricultural University.]
李建波,何璞.2017.我国食用菌双翅目害虫种类和防治研究现状[J].中国植保导刊,37(11):19-25.[Li J B,He P.2017.Research progress on diptera pest species of mushroom and its controlling in China[J].China Plant Protection,37(11):19-25.]
刘跃钧,王思贵,郑文彪,刘爱英,傅双凤.2003.秀珍菇生物学特性及高产栽培技术[J].食用菌,25(4):37-38.[Liu Y J,Wang S G,Zheng W B,Liu A Y,Fu S F.2003.The biological characteristics and high-yield culture technique of Pleurotus pulmonarius[J].Edible Fungi,25(4):37-38.]
罗佳,庄秋林.2007.福建食用菌双翅目害虫的种类、为害及防治[J].福建农林大学学报(自然科学版),36(3):237-240.[Luo J,Zhuang Q L.2007.The species,damage and control of Diptera pests in edible fungi in Fujian Province[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),36(3):237-240.]
马林,林金盛,曲绍轩,李辉平,侯立娟,蒋宁,宋金俤.2017.三种杀虫剂对从南京食用菌大棚随机捕获的Bradysia的防治效果[J].食用菌学报,24(3):87-90.[Ma L,Lin JS,Qu S X,Li H P,Hou L J,Jiang N,Song J D.2017.Efficacy of thiamethoxam,pymetrozine and acetamipridin controlling Bradysia under laboratory conditions and in a Pleurotus ostreatus cultivation facility[J].Acta Edulis Fungi,24(3):87-90.]
马林,曲绍轩,林金盛,宋金俤,李辉平,侯立娟,蒋宁.2015.食用菌迟眼蕈蚊生防菌苏云金芽孢杆菌的筛选及毒力测定[J].食用菌,(6):53-55.[Ma L,Qu S X,Lin J S,Song J D,Li H P,Hou L J,Jiang N.2015.Screening and toxicity test of biocontrol bacterium Bacillus thuringiensis against mushroom Bradysia[J].Edible Fungi,(6):53-55.]
曲绍轩,马林,华秀红,宋金俤.2010.古田山多菌蚊物理防治方法筛选试验[J].食用菌,(1):59-60.[Qu S X,Ma L,Hua X H,Song J D.2010.Screening tests of physical control methods against Docosia gutiuushana[J].Edible Fungi,(1):59-60.]
曲绍轩,马林,宋金俤,李辉平,华秀红.2012.食用菌眼蕈蚊的分子鉴定及2种植物源农药的室内毒力测定[J].江苏农业科学,40(9):123-125.[Qu S X,Ma L,Song J D,Li H P,Hua X H.2012.Molecular identification of mushroom sciarid flies and indoor toxicity tests of two botanical pesticide[J].Jiangsu Agricultural Sciences,40(9):123-125.]
沈登荣,张宏瑞,李正跃,和绍禹,张陶.2009.云南食用菌眼蕈蚊分类及优势种分析[J].昆虫学报,52(8):934-940.[Shen D R,Zhang H R,Li Z Y,He S Y,Zhang T.2009.Taxonomy and dominance analysis of sciarid fly species(Diptera:Sciaridae)on edible fungi in Yunnan[J].Acta Entomologica Sinica,52(8):934-940.]
沈迎春,张怡,钱忠海,刘琴,王林贵.2017.生物农药防治双孢蘑菇菇蚊菇蝇效果初探--以苏云金杆菌以色列亚种为例[J].农药科学与管理,38(11):47-51.[Shen Y C,Zhang Y,Qian Z H,Liu Q,Wang L G.2017.Preliminary exploration of the effect on biopesticide,such as Bacillus thuringiensis subsp.Israelensis,against mosquitoes and flies in Agaricus bisporus cultivation[J].Pesticide Science and Administration,38(11):47-51.]
师迎春,杨秀芬,张涛,胡彬,丁守付,戴宇婷.2014.苏云金芽孢杆菌制剂对双孢蘑菇栽培房眼蕈蚊的控制作用[J].食用菌学报,21(4):76-80.[Shi Y C,Yang X F,Zhang T,Hu B,Ding S F,Dai Y T.2014.Effectiveness of Bacillus thuringiensis microbial agents in controlling sciarid fly infestation in Agaricus bisporus cultivation rooms[J].Acta Edulis Fungi,21(4):76-80.]
宋爽,刘宇,王兰青,王守现,许峰,黄晨阳.2015.三种生物源农药对平菇双翅目害虫的药效试验[J].食用菌,(5):57-58.[Song S,Liu Y,Wang L Q,Wang S X,Xu F,Huang C Y.2015.Efficacy experiment of three biopesticides against diptera pest in Pleurotus ostreatus cultivation[J].Edible Fungi,(5):57-58.]
王丹丹.2012.平菇昆虫种群动态、群落特征和平菇厉眼蕈蚊内禀生物学特性研究[D].泰安:山东农业大学.[Wang D D.2012.Researches on insect population dynamics and community characteristics on Pleurotus ostreatus and intrinsic ecological characteristics of Lycoriella pleuroti Yang et Zhang[D].Tai’an:Shandong Agricultural University.]
王瑾.2013.南宁市平菇菇蚊菇蝇种类调查及物理防治技术研究[D].南宁:广西大学.[Wang J.2013.Species investigation and study of physical control of mosquitoes and flies on Pleurotus ostreatus in Nanning[D].Nanning:Guangxi University.]
温志强,边广.2011.秀珍菇菇蚊菇蝇的消长规律与物理防治技术[J].热带作物学报,32(10):1931-1934.[Wen ZQ,Bian G.2011.Preliminary study of variation and physical control techniques on mushroom flies of Pleurotus geesteranus Singer[J].Chinese Journal of Tropical Crops,32(10):1931-1934.]
徐汉虹,赖多,张志祥.2017.植物源农药印楝素的研究与应用[J].华南农业大学学报,38(4):1-11.[Xu H H,Lai D,Zhang Z X.2017.Research and application of botanical pesticide azadirachtin[J].Journal of South China A-gricultural University,38(4):1-11.]
张超逸.2017.沈阳地区平菇主要害虫种类调查和发生规律研究[D].沈阳:沈阳大学.[Zhang C Y.2017.The main pest species and dynamic regularity of oyster mushroom in Shenyang area[D].Shenyang:Shenyang University.]
张孔金,黎志银,谢宝贵,黄志龙.2012.不同农药对秀珍菇生长及产品安全性影响的研究[J].食药用菌,20(5):292-294.[Zhang K J,Li Z Y,Xie B G,Huang Z L.2012.The study on the effect of Pleurotus geesteranus growth and product safety on different pesticides[J].Edible and Medicinal Mushrooms,20(5):292-294.]
张晓云,沈登荣,李正跃,张陶,张宏瑞.2007.食用菌双翅目害虫的采集和玻片标本制作方法[J].中国食用菌,26(5):56-57.[Zhang X Y,Shen D R,Li Z Y,Zhang T,Zhang H R.2007.Collection and slide preparation methods of dipterous pests on edible mushrooms[J].Edible Fungi of China,26(5):56-57.]
赵晓娜,廖慧东,罗佳,吴梅香,范青海.2011.杀虫剂对黑腹果蝇毒力测定及对香菇和秀珍菇菌丝生长的影响[J].江西农业大学学报,33(2):283-286.[Zhao X N,Liao H D,Luo J,Wu M X,Fan Q H.2011.Toxicities of 4 insecticides to Drosophila melanogaster and their effcects on growth of Lentinus edodes and Pleurotu sgeesteranus[J].Acta Agriculturae Universitatis Jiangxiensis,33(2):283-286.]
Ross A,Willson V L.2017.Basic and Advanced Statistical Tests[M].Rotterdam:Sense Publishers:21-24.
Thonghua T,Thonghua J.2015.The efficacy of insecticidal control in Pomelo fruit fly IPM program in Nakhon Si Thammarat Province,Thailand[J].International Journal of Agricultural Technology,11(2):391-397.