ISSR分子标记在果蔬资源上的应用及研究进展
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摘要
ISSR分子标记是为了克服SSR引物设计费时费力的问题,在此发展起来的一种分子标记。其操作简单、高效,试验成本低,且多态性高、重复性好、引物设计简单。本文对几种常用分子标记的优缺点进行简单比较,重点介绍了目前广泛应用于果蔬资源研究中的ISSR分子标记的原理和特点,对ISSR分子标记技术在果蔬资源的遗传多样性和遗传结构、种质资源鉴定以及遗传图谱等方面的研究进展及应用进行综述,旨为果蔬资源的进一步研究利用提供参考依据。
Inter-simple sequence repeat(ISSR)molecular marker is one of molecular markers which is developed to overcome the time-consuming and laborious problem of SSR primer design.It has simple operation,high efficiency,low experimental cost,high polymorphism,good repeatability and simple primer design.The advantages and disadvantages of several common molecular markers were compared in the article,mainly introduced the principle and characteristics of ISSR molecular marker widely used in fruit and vegetable resources at present.The research progress and application of ISSR molecular marker in the genetic diversity,genetic structure,germplasm resources identification and genetic map of fruit and vegetable resources were reviewed.It aims to provide a reference for further research and utilization of fruit and vegetable resources.
Inter-simple sequence repeat(ISSR)molecular marker is one of molecular markers which is developed to overcome the time-consuming and laborious problem of SSR primer design.It has simple operation,high efficiency,low experimental cost,high polymorphism,good repeatability and simple primer design.The advantages and disadvantages of several common molecular markers were compared in the article,mainly introduced the principle and characteristics of ISSR molecular marker widely used in fruit and vegetable resources at present.The research progress and application of ISSR molecular marker in the genetic diversity,genetic structure,germplasm resources identification and genetic map of fruit and vegetable resources were reviewed.It aims to provide a reference for further research and utilization of fruit and vegetable resources.
引文
[1]刘淑芹,吴凤芝,刘守伟.园艺作物的ISSR分子标记研究及应用[J].东北农业大学学报,2012,43(4):145-150.
[2]许洹瑞.分子标记技术及其研究进展[J].黑龙江环境通报,2013,37(1):1-4.
[3]邹奕,马龙彪,江伟,等.ISSR分子标记技术在甜菜育种中的应用[J].中国糖料,2018,40(3):75-77.
[4]LACIS G,RASHAL I,TRAJKOVSKI V.Implementation of a limited set of SSR markers for screening of genetic variability in Latvian and Swedish sour cherry(Prunus cerasus L.)genetic resources collections[J].Proceedings of the Latvian Academy of Sciences,2011,65(1/2):21-28.
[5]SHARMA R,SHARMA S,KUMAR S.Pair-wise combinations of RAPD primers for diversity analysis with reference to protein and single primer RAPD in soybean[J].Annals of Agrarian Science,2018,16(3):243-249.
[6]SHUBHANGI S,PANKAJ K,GEETIKA G,et al.Assessment of genetic diversity in lettuce(Lactuca sativa L.)germplasm using RAPD markers[J].3 Biotech,2018,8(1):9.
[7]OUTRAM M A,JONES E E,JASPERS M V,et al.Development of a PCRRFLP method to distinguish species within the Ilyonectria macrodidyma complex[J].New Zealand Plant Protection,2014,67:151-156.
[8]PHARMAWATI M,IMANIAR E F.PCR-RFLP and sequencing of trnS/trnfM fragment of enhalus acoriodes from Sanur Coastal Waters,Bali,Indonesia:A preliminary study[J].Journal of Tropical Life Science,2016,6(2):118-122.
[9]JOHN B B,JOSEPH O O,GBOLAGADE J S.Molecular variability of Celosia argentea using amplified fragment length polymorphism(AFLP)marker[J].Molecular Plant Breeding,2016,26(7):1-6.
[10]MOHAMED A.ESAWI E L,GERMAINE K,et al.AFLP analysis of genetic diversity and phylogenetic relationships of Brassica oleracea in Ireland[J].Comptes Rendus-Biologies,2016,339(5/6)163-170.
[11]DASGUPTA N,NANDY P,SENGUPTA C,et al.Genetic variation in relation to adaptability of three mangrove species from the Indian Sundarbans assessed with RAPD and ISSR markers[J].Journal of Forestry Research,2018,29(2):301-310.
[12]ZHANG K X,WANG D,YANG C,et al.Linkage map construction and QTL analysis for Betula platyphylla Suk using RAPD,AFLP,ISSR and SSR[J].Silvae Genetica,2012,61(1):1-9.
[13]VICENTE S,MAGUAS C,TRINDADE H.Genetic diversity and differentiation of invasive Acacia longifolia in Portugal[J].Web Ecology,2018,18(1):91-103.
[14]JEPKOSGEI T E,OSORO O A,ANGIYO W B,et al.Molecular markers associated with alumini um tolerance in Sorghum bicolor[J].Hereditas,2018,155:20.
[15]林伟东,陈志丹,孙威江,等.基于SCoT标记的福建茶树品种(系)遗传多样性分析[J].茶叶科学,2018(1):43-57.
[16]郎彬彬,黄春辉,陶俊杰,等.毛花猕猴桃种质资源ISSR反应体系的建立和优化[J].分子植物育种,2076,14(2):511-516.
[17]陈河龙,高建明,张世清,等.芦笋ISSR反应体系的建立及优化[J].分子植物育种,2018,16(1):170-174.
[18]马丽,侯乐峰,郝兆祥,等.82个石榴品种遗传多样性的ISSR分析[J].果树学报,2015,32(5):741-750.
[19]陈名红,熊华斌,李成云.分子标记在百合属植物遗传多样性研究中的应用[J].生物技术通报,2011(12):65-69.
[20]STRASBURG J L,SHERMAN N A,WRIGHT K M,et al.What can patterns of differentiation across plant genomes tell us about adaptation and speciation?[J].Philosophical Transactions of the Royal Society B Biological Sciences,2012,367(1587):364-373
[21]YU H H,YANG Z L,SUN B,et al.Genetic diversity and relationship of endangered plant Magnolia officinalis(Magnoliaceae)assessed with ISSR polymorphisms[J].Biochemical Systematics&Ecology,2011,39(2):71-78.
[22]朱白婢,蔡兴来,周曼,等.基于ISSR标记的印度南瓜种质资源遗传多样性分析[J].分子植物育种,2016,14(12):3596-3600.
[23]MORENO S,MARTIN J P,ORTIZ J M.Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm[J].Euphytica,1998,101(1):117-125.
[24]张起,安华明.贵州砂梨种质资源遗传多样性的ISSR分析[J].果树学报,2015,32(1):6-12.
[25]李国田,张美勇,相昆,等.基于ISSR标记的16个核桃品种遗传多样性分析及分子身份构建[J].核农学报,2015,29(10):1884-1892.
[26]刘宝尧,刘小利,魏海斌,等.青海地方核桃种质资源遗传多样性研究[J].西北林学院学报,2017,32(2):130-135.
[27]李春侨,周龙,陆彪,等.天山樱桃野生居群遗传多样性ISSR分析[J].分子植物育种,2017,15(6):2420-2426.
[28]李涛,黎振兴,罗少波,等.东南亚茄子种质资源ISSR遗传多样性分析[J].中国农学通报,2014,30(25):104-110.
[29]肖熙鸥,王勇,李冠男,等.茄子种质资源的ISSR遗传多样性分析[J].华南农业大学学报,2012,33(3):296-300.
[30]陆贵峰,黄川,刘钰,等.24份古荔枝种质资源ISSR遗传多样性分析[J].南方农业学报,2017,48(2):197-201.
[31]梁敏,董文轩,甄睿,等.桃李杏资源亲缘关系的ISSR分析[J].贵州农业科学,2011,39(5):27-31.
[32]李雪峤,高芳华,伍壮生,等.基于ISSR标记的酱用型灯笼椒种质资源遗传多样性分析[J].分子植物育种,2018,16(5):1711-1716.
[33]王庆军,罗华,赵丽娜,等.24个山东石榴品种遗传多样性的ISSR分析[J].中国果树,2018(2):18-22.
[34]马丽,郝兆祥,周玉亮,等.果用石榴品种的ISSR遗传多样性分析[J].北方园艺,2016(21):106-110.
[35]王美军,黄乐,刘昆玉,等.刺葡萄ISSR分子标记体系的建立及种质资源聚类分析[J].果树学报,2017,34(3):279-287.
[36]陈小宇,刘林德,葛宜和,等.‘蛇龙珠’葡萄新株系遗传多样性的SSR、ISSR和VvmybA1基因片段分析[J].果树学报,2017,34(2):145-156.
[37]程琴,庞新华,吕平,等.两个桂热甘蔗品种(系)的简单重复序列间扩增(ISSR)分子鉴别[J].科学技术与工程,2018,18(8):191-195.
[38]赵依杰,王江波,张小红,等.枇杷新品种‘东湖早’的ISSR分子鉴定[J].热带作物学报,2010,31(1):72-76.
[39]任梦云,陈彦君,张盾,等.ISSR标记技术在药用植物资源中的研究进展及应用[J].生物技术通报,2017,33(4):63-69.
[40]郑姗,张小艳,张立杰,等.40个龙眼品种(品系)DNA指纹图谱构建及遗传关系分析[J].中国农学通报,2016,32(1):107-112.
[41]尹明华,肖芳,糜俊,等.应用ISSR指纹图谱鉴定上饶早梨3个主栽品种及其不同继代次数试管苗[J].基因组学与应用生物学,2016,35(10):2842-2850.
[42]周坤华,陈学军,方荣,等.辣椒种间(Capsicum annuum×C. frutescens)遗传图谱的构建与分析[J].园艺学报,2013,40(11):2171-2179.
[43]吴国林.基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D].南昌:江西农业大学,2014.
[44]林伟东,孙威江,陈志丹.ISSR分子标记技术在茶树育种中的研究进展[J].广东农业科学,2015,42(23):133-138.
[2]许洹瑞.分子标记技术及其研究进展[J].黑龙江环境通报,2013,37(1):1-4.
[3]邹奕,马龙彪,江伟,等.ISSR分子标记技术在甜菜育种中的应用[J].中国糖料,2018,40(3):75-77.
[4]LACIS G,RASHAL I,TRAJKOVSKI V.Implementation of a limited set of SSR markers for screening of genetic variability in Latvian and Swedish sour cherry(Prunus cerasus L.)genetic resources collections[J].Proceedings of the Latvian Academy of Sciences,2011,65(1/2):21-28.
[5]SHARMA R,SHARMA S,KUMAR S.Pair-wise combinations of RAPD primers for diversity analysis with reference to protein and single primer RAPD in soybean[J].Annals of Agrarian Science,2018,16(3):243-249.
[6]SHUBHANGI S,PANKAJ K,GEETIKA G,et al.Assessment of genetic diversity in lettuce(Lactuca sativa L.)germplasm using RAPD markers[J].3 Biotech,2018,8(1):9.
[7]OUTRAM M A,JONES E E,JASPERS M V,et al.Development of a PCRRFLP method to distinguish species within the Ilyonectria macrodidyma complex[J].New Zealand Plant Protection,2014,67:151-156.
[8]PHARMAWATI M,IMANIAR E F.PCR-RFLP and sequencing of trnS/trnfM fragment of enhalus acoriodes from Sanur Coastal Waters,Bali,Indonesia:A preliminary study[J].Journal of Tropical Life Science,2016,6(2):118-122.
[9]JOHN B B,JOSEPH O O,GBOLAGADE J S.Molecular variability of Celosia argentea using amplified fragment length polymorphism(AFLP)marker[J].Molecular Plant Breeding,2016,26(7):1-6.
[10]MOHAMED A.ESAWI E L,GERMAINE K,et al.AFLP analysis of genetic diversity and phylogenetic relationships of Brassica oleracea in Ireland[J].Comptes Rendus-Biologies,2016,339(5/6)163-170.
[11]DASGUPTA N,NANDY P,SENGUPTA C,et al.Genetic variation in relation to adaptability of three mangrove species from the Indian Sundarbans assessed with RAPD and ISSR markers[J].Journal of Forestry Research,2018,29(2):301-310.
[12]ZHANG K X,WANG D,YANG C,et al.Linkage map construction and QTL analysis for Betula platyphylla Suk using RAPD,AFLP,ISSR and SSR[J].Silvae Genetica,2012,61(1):1-9.
[13]VICENTE S,MAGUAS C,TRINDADE H.Genetic diversity and differentiation of invasive Acacia longifolia in Portugal[J].Web Ecology,2018,18(1):91-103.
[14]JEPKOSGEI T E,OSORO O A,ANGIYO W B,et al.Molecular markers associated with alumini um tolerance in Sorghum bicolor[J].Hereditas,2018,155:20.
[15]林伟东,陈志丹,孙威江,等.基于SCoT标记的福建茶树品种(系)遗传多样性分析[J].茶叶科学,2018(1):43-57.
[16]郎彬彬,黄春辉,陶俊杰,等.毛花猕猴桃种质资源ISSR反应体系的建立和优化[J].分子植物育种,2076,14(2):511-516.
[17]陈河龙,高建明,张世清,等.芦笋ISSR反应体系的建立及优化[J].分子植物育种,2018,16(1):170-174.
[18]马丽,侯乐峰,郝兆祥,等.82个石榴品种遗传多样性的ISSR分析[J].果树学报,2015,32(5):741-750.
[19]陈名红,熊华斌,李成云.分子标记在百合属植物遗传多样性研究中的应用[J].生物技术通报,2011(12):65-69.
[20]STRASBURG J L,SHERMAN N A,WRIGHT K M,et al.What can patterns of differentiation across plant genomes tell us about adaptation and speciation?[J].Philosophical Transactions of the Royal Society B Biological Sciences,2012,367(1587):364-373
[21]YU H H,YANG Z L,SUN B,et al.Genetic diversity and relationship of endangered plant Magnolia officinalis(Magnoliaceae)assessed with ISSR polymorphisms[J].Biochemical Systematics&Ecology,2011,39(2):71-78.
[22]朱白婢,蔡兴来,周曼,等.基于ISSR标记的印度南瓜种质资源遗传多样性分析[J].分子植物育种,2016,14(12):3596-3600.
[23]MORENO S,MARTIN J P,ORTIZ J M.Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm[J].Euphytica,1998,101(1):117-125.
[24]张起,安华明.贵州砂梨种质资源遗传多样性的ISSR分析[J].果树学报,2015,32(1):6-12.
[25]李国田,张美勇,相昆,等.基于ISSR标记的16个核桃品种遗传多样性分析及分子身份构建[J].核农学报,2015,29(10):1884-1892.
[26]刘宝尧,刘小利,魏海斌,等.青海地方核桃种质资源遗传多样性研究[J].西北林学院学报,2017,32(2):130-135.
[27]李春侨,周龙,陆彪,等.天山樱桃野生居群遗传多样性ISSR分析[J].分子植物育种,2017,15(6):2420-2426.
[28]李涛,黎振兴,罗少波,等.东南亚茄子种质资源ISSR遗传多样性分析[J].中国农学通报,2014,30(25):104-110.
[29]肖熙鸥,王勇,李冠男,等.茄子种质资源的ISSR遗传多样性分析[J].华南农业大学学报,2012,33(3):296-300.
[30]陆贵峰,黄川,刘钰,等.24份古荔枝种质资源ISSR遗传多样性分析[J].南方农业学报,2017,48(2):197-201.
[31]梁敏,董文轩,甄睿,等.桃李杏资源亲缘关系的ISSR分析[J].贵州农业科学,2011,39(5):27-31.
[32]李雪峤,高芳华,伍壮生,等.基于ISSR标记的酱用型灯笼椒种质资源遗传多样性分析[J].分子植物育种,2018,16(5):1711-1716.
[33]王庆军,罗华,赵丽娜,等.24个山东石榴品种遗传多样性的ISSR分析[J].中国果树,2018(2):18-22.
[34]马丽,郝兆祥,周玉亮,等.果用石榴品种的ISSR遗传多样性分析[J].北方园艺,2016(21):106-110.
[35]王美军,黄乐,刘昆玉,等.刺葡萄ISSR分子标记体系的建立及种质资源聚类分析[J].果树学报,2017,34(3):279-287.
[36]陈小宇,刘林德,葛宜和,等.‘蛇龙珠’葡萄新株系遗传多样性的SSR、ISSR和VvmybA1基因片段分析[J].果树学报,2017,34(2):145-156.
[37]程琴,庞新华,吕平,等.两个桂热甘蔗品种(系)的简单重复序列间扩增(ISSR)分子鉴别[J].科学技术与工程,2018,18(8):191-195.
[38]赵依杰,王江波,张小红,等.枇杷新品种‘东湖早’的ISSR分子鉴定[J].热带作物学报,2010,31(1):72-76.
[39]任梦云,陈彦君,张盾,等.ISSR标记技术在药用植物资源中的研究进展及应用[J].生物技术通报,2017,33(4):63-69.
[40]郑姗,张小艳,张立杰,等.40个龙眼品种(品系)DNA指纹图谱构建及遗传关系分析[J].中国农学通报,2016,32(1):107-112.
[41]尹明华,肖芳,糜俊,等.应用ISSR指纹图谱鉴定上饶早梨3个主栽品种及其不同继代次数试管苗[J].基因组学与应用生物学,2016,35(10):2842-2850.
[42]周坤华,陈学军,方荣,等.辣椒种间(Capsicum annuum×C. frutescens)遗传图谱的构建与分析[J].园艺学报,2013,40(11):2171-2179.
[43]吴国林.基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D].南昌:江西农业大学,2014.
[44]林伟东,孙威江,陈志丹.ISSR分子标记技术在茶树育种中的研究进展[J].广东农业科学,2015,42(23):133-138.