寿光鸡AANAT基因5′调控区的单核苷酸多态性及其与产蛋性状的关联分析
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摘要
褪黑激素(melatonin,Mel)分泌增加可阻碍家禽生殖器官的发育,降低家禽产蛋量。5-羟色胺-N-乙酰基转移酶(arylalkylamine N-acetyltransferase,AANAT)是Mel合成过程中的限速酶。本实验旨在研究AANAT基因5'调控区多态性及其与寿光鸡(Gallus domesticus'Shouguang')产蛋性能的关系,采用直接测序法检测了AANAT基因5'调控区的SNPs,并将检测到的SNPs与寿光鸡的产蛋性状进行关联分析。结果表明,AANAT基因5'控区共21个SNPs,其中10个低度多态、11个中度多态。3个中度多态位点对产蛋性能影响显著(P<0.05),其中-803位点能使开产日龄显著提前,并能使前期(20~25周龄)产蛋数显著增加;-561位点能使中期产蛋数显著增加;-522位点能使后期(46~57周龄)产蛋数显著增加;-561、-522位点能使产蛋总数显著增加。综上所述,AANAT基因5'调控区有多个SNPs对产蛋性状影响显著,可作为寿光鸡产蛋性状辅助选择的有潜在利用价值的分子遗传标记。
The increase of melatonin(Mel) can obstruct the development of reproductive organs and reduce the egg production in poultry. The arylalkylamine N-acetyltransferase(AANAT) is the major rate-limiting enzyme for melatonin synthesis from serotonin. In order to investigate the polymorphism of the AANAT gene on laying traits, SNPs in 5' regulatory region of the gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Shouguang chickens(Gallus domesticus). The results showed that 10 low polymorphism loci and 11 moderate polymorphism loci were found in the 5' regulatory region, respectively. Three SNPs with moderate polymorphisms had significant effects on laying traits(P<0.05). The locus of-803 could significantly advance the age at first egg and increase the egg production during 20~25 weeks of age. The loci of-561 and-522 increased the egg production during 26~45 weeks of age and during 46~57 weeks of age, respectively, so as to significantly increased the total egg production as well. In summary, the 5' regulatory region of AANAT gene have many SNPs, which were significantly associated with laying traits and thus are potential molecular markers to improve laying traits in Shouguang chickens.
The increase of melatonin(Mel) can obstruct the development of reproductive organs and reduce the egg production in poultry. The arylalkylamine N-acetyltransferase(AANAT) is the major rate-limiting enzyme for melatonin synthesis from serotonin. In order to investigate the polymorphism of the AANAT gene on laying traits, SNPs in 5' regulatory region of the gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Shouguang chickens(Gallus domesticus). The results showed that 10 low polymorphism loci and 11 moderate polymorphism loci were found in the 5' regulatory region, respectively. Three SNPs with moderate polymorphisms had significant effects on laying traits(P<0.05). The locus of-803 could significantly advance the age at first egg and increase the egg production during 20~25 weeks of age. The loci of-561 and-522 increased the egg production during 26~45 weeks of age and during 46~57 weeks of age, respectively, so as to significantly increased the total egg production as well. In summary, the 5' regulatory region of AANAT gene have many SNPs, which were significantly associated with laying traits and thus are potential molecular markers to improve laying traits in Shouguang chickens.
引文
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贾祥捷,王长法,李秋玲,等.2011.中国荷斯坦牛POU1F1基因与PRL基因的多态性及其聚合效应对产奶性状的影响[J].遗传,33(12):1359-1365.(Jia X J,Wang CF,Li Q L,et al.2011.Polymorphism of POU1F1 gene and PRL gene and their combined effects on milk performance traits in Chinese Holstein cattle[J].Hereditas,33(12):1359-1365.)
周敏,和俊,谢袖娟,等.2012.VIPR-1基因5'调控区多态位点与鸡就巢性的关联分析[J].畜牧兽医学报,43(3):343-352.(Zhou M,He J,Xie X J,et al.2012.Association of polymorphisms in the 5'regulator region of the VIPR-1 gene with chicken broodiness[J].Acta Veterinaria et Zootechnica Sinica,43(3):343-352.)
宋倩,王慧,康丽,等.2013.寿光鸡褪黑素受体基因(MTNR1A)5'调控区多态性与产蛋性状的关联分析[J].农业生物技术学报,05:579-587.(Song Q,Wang H,Kang L,et al.2013.Polymorphisms of the 5'regulatory region of melatonin receptor gene(MTNR1A)and their associations with laying traits in Shouguang chickens(Gallus gallus)[J].Journal of Agricultural Biotechnology,05:579-587.)
于成江.2007.常年性和季节性发情绵羊AA-NAT和HIOMT基因遗传多态性的研究[D].硕士学位论文,西北农林科技大学,陈玉林;尹君亮,pp.35-36.(Yu CJ.2007.Studies on genetic polymorphisim of AANATand HIOMT genes in nonseasonnal and seasonal oestrus sheep[D].Thesis for M.S.,Northwest A&F University.Suppervisor:Chen Y L;Yin J L,pp.35-36.)
杜朝,玉蕾叶,孙国杰,等.2014.真核表达载体pc DNA3.1(+)-Myo D-HA-His的构建及功能检测[J].河北科技大学学报,02:149-153.(Du C,Yu L Y,Sun G J,et al.2014.Construction and effect test of the eukaryotic expression vedtor pc DNA3.1(+)-Myo D-HA-His[J].Journal of Hebei University of Science and Technology,02:49-153.)
华登科,贺海军,贾亚雄,等.2014.光照强度对北京油鸡生长激素和褪黑激素含量的影响[J].中国家禽,36(19):30-32.(Hua D K,He H J,Jia Y X et al.2014.Effect of light intensity on growth hormone and melatonin secretion of Beijing You chicken[J].China Poultry,36(19):30-32.)
Gilad E,Laudon M,Matzkin H,et al.1998.Evidence for a local action of melatonin on the rat prostate[J].Urology,159(3):1069-1073.
Foulker N S,Borjigin J,Snyder S H,et al.1997.Rhythmic transcription:the molecular basis of circadian melatonin synthesis[J].Biology of the Cell,89(8):487-494.
Klein D C,Coon S L,Roseboom P H,et al.1997.The melato nin rhythm-generating enzyme:Molecular regulation o serotonin N-acetyltransferase in the prineal gland[J]Recent Progress in Hormone Research,52:307-357;dis cussion 357-358.
Hardeland R,Poeggeler B.2003.Non-Vertebrate melatonin[J].Journal of Pineal Research,34(4):233-241.
Chowdhur I,Sengupta A,Maitra S K.2008.Melatonin:Fifty years of scientific journey from the discovery in bovin pineal gland to delineation of functions in human[J].In dian Journal of Biochemistry and Biophysics,45(5)289-304.
Qiu X M,Li N,Deng X M,et al.2006.The single nucleotid polymorphisms of chicken melanocortin-4 recepto(MC4R)gene and their association nanlysis with carcas traits[J].Science in China Series C:Life Science,49(6):560-566.
Sekine A,Saito S,Iida A,et al.2001.Identification of single nucleotide polymorphisms(SNPs)of human N-acetyl transferase genes NAT1,NAT2,AANAT,ARD1,and L1CAM in the Japanese population[J].Journal of Human Genetics,46(4):314-319.
Hohjoh H,Takasu M,Shishikura K,et al.2003.Significant as sociation of the arylalkylamine N-acetyltransferase(AA-NAT)gene with delayed sleep phase syndrome[J].Neu rogenetics,4(3):151-153.
何兰花,田允波,薛立群,等.2004.褪黑激素被动免疫对母鸭卵泡发育和垂体激素水平的影响[J].佛山科学技术学院学报(自然科学版),22(3):67-70.(He L H,Tian YB,Xue L Q,et al.2004.Effect of melatonin passive im munization on the follicle development and pituitary hormone of female ducks[J].Journal of Foshan Univer sity(Natural Science Edition),22(3):67-70.)
闫艳.2010.与山羊繁殖性能相关候选基因的研究[D].硕士学位论文,重庆大学,导师:王凭青;储明星,pp.6065.(Yan Y.2010.Study on candidate gene related to breeding performance of goats[D].Thesis for M.S.Chongqing University,Suppervisor:Wang P Q;Chu MX,pp.60-65.)
赵雪丹,王慧,康丽,等.2014.褪黑激素受体MTNR1B 5′调控区多态及其与鸡产蛋性状的关联分析[J].中国兽医学报,11:1855-1860.(Zhao X D,Wang H,Kang L et al.2014.Polymorphisms of the 5′regulatory reagion of melatonin receptor gene MTNR1B and their correlations with laying traits in chickens[J].Chinese Journal of Veterinary Science,11:1855-1860.)
李晓宁,王慧,于希江,等.2012.文昌鸡MTNR1B基因的多态性及其与产蛋性状的关系.畜牧兽医学报,43(4):540-545.(Li X N,Wang H,Yu X J et al.2012.Polymorphisms of MTNR1B gene and its association with laying traits in Wenchang chickens[J].Acta Veterinaria et Zootechnica Sinica,43(4):540-545.)
雷晓玉,雷晓峰,赵晏等.2008.褪黑激素主动免疫对蛋鸡产蛋率的影响[J].畜禽业,2008,4,228:10-12.(Lei X Y,Lei X F,Zhao Y et al.2008.The effect of melatonin active immune on rate of layers[J].Livestock and Poultry Industry,4(228):10-12.)
辛海瑞,潘晓花,毕晔,等.2016.光照节律对北京鸭生产性能、屠宰性能和血液抗氧化功能的影响[J].中国农业科学,49(23):4638-4645.(Xin H R,Pan X H,Bi Y et al.2016.Effects of lighting regimes on production performance,carcass performance and anti-oxidant capacity of the blood in Peking ducks[J].Scientia Agricultura Sinica,49(23):4638-4645.)
贾祥捷,王长法,李秋玲,等.2011.中国荷斯坦牛POU1F1基因与PRL基因的多态性及其聚合效应对产奶性状的影响[J].遗传,33(12):1359-1365.(Jia X J,Wang CF,Li Q L,et al.2011.Polymorphism of POU1F1 gene and PRL gene and their combined effects on milk performance traits in Chinese Holstein cattle[J].Hereditas,33(12):1359-1365.)
周敏,和俊,谢袖娟,等.2012.VIPR-1基因5'调控区多态位点与鸡就巢性的关联分析[J].畜牧兽医学报,43(3):343-352.(Zhou M,He J,Xie X J,et al.2012.Association of polymorphisms in the 5'regulator region of the VIPR-1 gene with chicken broodiness[J].Acta Veterinaria et Zootechnica Sinica,43(3):343-352.)
宋倩,王慧,康丽,等.2013.寿光鸡褪黑素受体基因(MTNR1A)5'调控区多态性与产蛋性状的关联分析[J].农业生物技术学报,05:579-587.(Song Q,Wang H,Kang L,et al.2013.Polymorphisms of the 5'regulatory region of melatonin receptor gene(MTNR1A)and their associations with laying traits in Shouguang chickens(Gallus gallus)[J].Journal of Agricultural Biotechnology,05:579-587.)
于成江.2007.常年性和季节性发情绵羊AA-NAT和HIOMT基因遗传多态性的研究[D].硕士学位论文,西北农林科技大学,陈玉林;尹君亮,pp.35-36.(Yu CJ.2007.Studies on genetic polymorphisim of AANATand HIOMT genes in nonseasonnal and seasonal oestrus sheep[D].Thesis for M.S.,Northwest A&F University.Suppervisor:Chen Y L;Yin J L,pp.35-36.)
杜朝,玉蕾叶,孙国杰,等.2014.真核表达载体pc DNA3.1(+)-Myo D-HA-His的构建及功能检测[J].河北科技大学学报,02:149-153.(Du C,Yu L Y,Sun G J,et al.2014.Construction and effect test of the eukaryotic expression vedtor pc DNA3.1(+)-Myo D-HA-His[J].Journal of Hebei University of Science and Technology,02:49-153.)
华登科,贺海军,贾亚雄,等.2014.光照强度对北京油鸡生长激素和褪黑激素含量的影响[J].中国家禽,36(19):30-32.(Hua D K,He H J,Jia Y X et al.2014.Effect of light intensity on growth hormone and melatonin secretion of Beijing You chicken[J].China Poultry,36(19):30-32.)
Gilad E,Laudon M,Matzkin H,et al.1998.Evidence for a local action of melatonin on the rat prostate[J].Urology,159(3):1069-1073.
Foulker N S,Borjigin J,Snyder S H,et al.1997.Rhythmic transcription:the molecular basis of circadian melatonin synthesis[J].Biology of the Cell,89(8):487-494.
Klein D C,Coon S L,Roseboom P H,et al.1997.The melato nin rhythm-generating enzyme:Molecular regulation o serotonin N-acetyltransferase in the prineal gland[J]Recent Progress in Hormone Research,52:307-357;dis cussion 357-358.
Hardeland R,Poeggeler B.2003.Non-Vertebrate melatonin[J].Journal of Pineal Research,34(4):233-241.
Chowdhur I,Sengupta A,Maitra S K.2008.Melatonin:Fifty years of scientific journey from the discovery in bovin pineal gland to delineation of functions in human[J].In dian Journal of Biochemistry and Biophysics,45(5)289-304.
Qiu X M,Li N,Deng X M,et al.2006.The single nucleotid polymorphisms of chicken melanocortin-4 recepto(MC4R)gene and their association nanlysis with carcas traits[J].Science in China Series C:Life Science,49(6):560-566.
Sekine A,Saito S,Iida A,et al.2001.Identification of single nucleotide polymorphisms(SNPs)of human N-acetyl transferase genes NAT1,NAT2,AANAT,ARD1,and L1CAM in the Japanese population[J].Journal of Human Genetics,46(4):314-319.
Hohjoh H,Takasu M,Shishikura K,et al.2003.Significant as sociation of the arylalkylamine N-acetyltransferase(AA-NAT)gene with delayed sleep phase syndrome[J].Neu rogenetics,4(3):151-153.