质谱仪在微生物检验临床教学中的实践
摘要
为提高质谱仪在病原体鉴定中应用的教学,探讨将翻转课堂导入教学环节,使繁琐复杂的微生物检验教学变得引人入胜,提高实验室的教学水平,提高学生的临床综合分析能力。在带教教师的指导下,进修和实习学生通过鉴定结果和相关的临床病例进行初步分析和探讨,在微生物检验的临床工作中掌握实验室相关的应用与实践,因此本文将进行通过导入翻转课堂,对初学者进行质谱仪的临床技能操作与考核的相关研究。
引文
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[10]LEE C,KIM S W.Effectiveness of a flipped classroom in learning periodontal diagnosis and treatment planning[J].J Dent Educ,2018,82(6):614-620.
[2]RATCLIFFE P,FANG H,THIDHOLM E,et al.Comparison of MALDI-TOF MS and VITEK 2system for laboratory diagnosis of Granulicatella and Abiotrophia species causing invasive infections[J].Diagn Microbiol Infect Dis,2013,77(3):216-219.
[3]FLORIO W,TAVANTI A,BARNINI S,et al.Recent advances and ongoing challenges in the diagnosis of microbial infections by MALDI-TOF mass spectrometry[J].Front Microbiol,2018,9(9):1097-1106.
[4]CASSAGNE C,NORMAND A C,L′OLLIVIER C,et al.Performance of MALDI-TOF MS platforms for fungal identification[J].Mycoses,2016,59(11):678-690.
[5]GARNER O,MOCHON A,BRANDA J,et al.Multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the VITEK R MS system[J].Clin Microbiol Infect,2014,20(4):335-339.
[6]MATHER C A,RIVERA S F,BUTLER-WU S M.Comparison of the bruker biotyper and vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of mycobacteria using simplified protein extraction protocols[J].J Clin Microbiol,2014,52(1):130-138.
[7]LAU S K,TANG B S,TENG J L,et al.Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of clinically significant bacteria that are difficult to identify in clinical laboratories[J].J Clin Pathol,2014,67(4):361-366.
[8]BROYER P,PERROT N,ROSTAING H,et al.An automated sample preparation instrument to accelerate positive blood cultures microbial identification by MALDI-TOF mass spectrometry(vitek(R)MS)[J].Front Microbiol,2018,9(9):911-925.
[9]SCHUBERT S,KOSTRZEWA M.MALDI-TOF MS in the microbiology laboratory:current trends[J].Curr Issues Mol Biol,2017(23):17.
[10]LEE C,KIM S W.Effectiveness of a flipped classroom in learning periodontal diagnosis and treatment planning[J].J Dent Educ,2018,82(6):614-620.