人参花中人参皂苷Re和Rg1成分提取工艺优选
|
摘要
目的:通过正交试验设计方法优选人参花中人参皂苷Re和Rg1成分的提取工艺,为合理开发利用人参花提供科学依据。方法:采用RRLC-Q-TOF MS方法定量测定人参皂苷Re和Rg1的含量。以人参花中人参皂苷Re和Rg1含量为检测指标,以料液比、提取次数和提取时间为因素,采用L9(34)正交试验设计法优化人参花中人参皂苷Re和Rg1成分的提取工艺,从而确定人参花口服液的最佳提取工艺。结果:人参花水提液以人参皂苷Re和Rg1含量为指标测定,最佳提取工艺为料液比1∶12,提取次数为3次,提取时间为1h。结论:优选出的提取工艺稳定、合理,有效成分提取率高。
Objective:To optimize the extraction process of ginsenosides Re and Rg1 from ginseng flowers by taking the content of ginsenoside Re and Rg1 as index.Methods:Taking the content of ginsenoside Re and Rg1 as index,orthogonal test was adopted to optimize extraction process of ginsenoside Re and Rg1 from ginseng flowers by taking material-liquid ratio,extraction times and extraction time as factors.The content of ginsenoside Re and Rg1 was determined by RRLC-Q-TOF MS.Results:The optimal extraction conditions are as follows:solid-liquid ratio is 1∶12,extraction time is 1 h,and extract 3 times.Conclusion:The verification test shows that the optimized extraction process is stable,reasonable and has high yield of active constituents.
Objective:To optimize the extraction process of ginsenosides Re and Rg1 from ginseng flowers by taking the content of ginsenoside Re and Rg1 as index.Methods:Taking the content of ginsenoside Re and Rg1 as index,orthogonal test was adopted to optimize extraction process of ginsenoside Re and Rg1 from ginseng flowers by taking material-liquid ratio,extraction times and extraction time as factors.The content of ginsenoside Re and Rg1 was determined by RRLC-Q-TOF MS.Results:The optimal extraction conditions are as follows:solid-liquid ratio is 1∶12,extraction time is 1 h,and extract 3 times.Conclusion:The verification test shows that the optimized extraction process is stable,reasonable and has high yield of active constituents.
引文
[1]王恩鹏.人参花挥发油的质谱研究[D].长春:长春中医药大学,2010.
[2]郑维玲.人参花总皂苷对心血管系统影响的试验研究[D].长春:长春中医药大学,2009.
[3]王冠.人参花蕾中人参皂苷的分离纯化与生物转化[D].北京:北京化工大学,2010.
[4]杨慧洁,杨世海,庞立杰.人参花蕾研究进展[J].人参研究,2013,1(1):56-57.
[5]于永利,杨贵贞.吉林人参花总皂苷对NKC-IFN-IL-2调节网的作用及其抑瘤效应[J].中国免疫学杂志,1987,3(1):41-45.
[6]邢德君,杜葵琴,孙连坤,等.人参花蕾皂苷对失血性休克犬心、肝、肺组织SOD及五种金属元素含量的影响[J].中国病理生理杂志,1995,11(1):83-85.
[7]SHIVAKUMAR B R,KOLLURI S V R,RAVINDRANATHV.Glutathione homeostasis in brain during reperfusion following bilateral carotid artery occuLusion in the rat[J].Journal MolecuLar Cell Biology,1992,111:125-129.
[8]林楠,钟琳琳,李娜,等.人参花提取物抗疲劳作用的研究[J].长春中医药大学学报,2010,26(3):336-337.
[9]LI F,LV C N,LI Q,et al.Chemical and bioactive comparison of flowers of Panax gin-seng Meyer,Panax quinquefolius L.,and Panax notoginseng Burk[J].Journal of Ginseng Research,2017,41(4):487-495.
[10]QIU S,YANG W Z,YAO C L,et al.Malonylginsenosides with potential antidiabet-ic activities from the flower buds of Panax ginseng[J].Journal of Natural Products,2017,80(4):899-908.
[11]姜红柳.人参皂苷Re对老年性痴呆小鼠的治疗作用及机制研究[D].长春:吉林大学,2008.
[12]李玺,张欣,袁海峰,等.人参皂苷Rg1对AD模型大鼠脑片P-Tau,caspase-3表达的影响[J].中国中药杂志,2010,35(3):369-372.
[2]郑维玲.人参花总皂苷对心血管系统影响的试验研究[D].长春:长春中医药大学,2009.
[3]王冠.人参花蕾中人参皂苷的分离纯化与生物转化[D].北京:北京化工大学,2010.
[4]杨慧洁,杨世海,庞立杰.人参花蕾研究进展[J].人参研究,2013,1(1):56-57.
[5]于永利,杨贵贞.吉林人参花总皂苷对NKC-IFN-IL-2调节网的作用及其抑瘤效应[J].中国免疫学杂志,1987,3(1):41-45.
[6]邢德君,杜葵琴,孙连坤,等.人参花蕾皂苷对失血性休克犬心、肝、肺组织SOD及五种金属元素含量的影响[J].中国病理生理杂志,1995,11(1):83-85.
[7]SHIVAKUMAR B R,KOLLURI S V R,RAVINDRANATHV.Glutathione homeostasis in brain during reperfusion following bilateral carotid artery occuLusion in the rat[J].Journal MolecuLar Cell Biology,1992,111:125-129.
[8]林楠,钟琳琳,李娜,等.人参花提取物抗疲劳作用的研究[J].长春中医药大学学报,2010,26(3):336-337.
[9]LI F,LV C N,LI Q,et al.Chemical and bioactive comparison of flowers of Panax gin-seng Meyer,Panax quinquefolius L.,and Panax notoginseng Burk[J].Journal of Ginseng Research,2017,41(4):487-495.
[10]QIU S,YANG W Z,YAO C L,et al.Malonylginsenosides with potential antidiabet-ic activities from the flower buds of Panax ginseng[J].Journal of Natural Products,2017,80(4):899-908.
[11]姜红柳.人参皂苷Re对老年性痴呆小鼠的治疗作用及机制研究[D].长春:吉林大学,2008.
[12]李玺,张欣,袁海峰,等.人参皂苷Rg1对AD模型大鼠脑片P-Tau,caspase-3表达的影响[J].中国中药杂志,2010,35(3):369-372.