红参中5种人参皂苷含量的超高效液相色谱法同时测定
|
摘要
目的建立超高效液相色谱法同时测定红参中人参皂苷Rg_1、Re、Rf、Rb_1和Ro含量测定的方法。方法采用Waters Acquity UPLC BEH C18色谱柱(2.1×100 mm, 1.7μm),流动相为乙腈-0.05%磷酸溶液梯度洗脱,流速0.4ml·min~(-1),检测波长为203nm,柱温为40℃。结果 5种人参皂苷在相应的范围内呈良好的线性关系(r≥0.9999),平均加样回收率(n=9)分别为98.85%、98.42%、98.51%、99.63%和98.80%,RSD分别为1.2%、1.5%、1.3%、0.8%和0.9%。结论该方法操作简单,高效,准确,可靠,可用于红参的质量控制。
To establish a UPLC method for the simultaneous determination of ginsenoside Rg_1, Re, Rf, Rb_1 and Ro in red ginseng. The analysis was performed on a Waters Acquity UPLC BEH C18 column(2.1×100 mm, 1.7μm),using acetonitrile-0.05% phosphoric acid aqueous as the mobile phase in a gradient mode at a flow rate of 0.4 ml·min~(-1). The detection wavelength was 203 nm and column temperature was 40℃. The five kinds of ginsenosides in the corresponding linear ranges showed good linear relationships(r≥0.9999); the average recoveries(n=9) were 98.85%,98.42%,98.51%,99.63% and 98.80%,RSD were 1.2%,1.5%,1.3%,0.8% and 0.9%.The established method is simple, efficient, accurate and reliable, which can be used as a method of quality control of red ginseng.
To establish a UPLC method for the simultaneous determination of ginsenoside Rg_1, Re, Rf, Rb_1 and Ro in red ginseng. The analysis was performed on a Waters Acquity UPLC BEH C18 column(2.1×100 mm, 1.7μm),using acetonitrile-0.05% phosphoric acid aqueous as the mobile phase in a gradient mode at a flow rate of 0.4 ml·min~(-1). The detection wavelength was 203 nm and column temperature was 40℃. The five kinds of ginsenosides in the corresponding linear ranges showed good linear relationships(r≥0.9999); the average recoveries(n=9) were 98.85%,98.42%,98.51%,99.63% and 98.80%,RSD were 1.2%,1.5%,1.3%,0.8% and 0.9%.The established method is simple, efficient, accurate and reliable, which can be used as a method of quality control of red ginseng.
引文
[1] 国家药典委员会.中国药典,一部[S].北京:中国医药科技出版社,2010 : 143
[2] 徐绥绪, 王乃利,李英辉. 中国红参化学成分的研究(Ⅱ)[J]. 药学学报, 1986 , 21(5) : 356
[3] 刘丹,濮社班,钱士辉, 等.中国红参化学成分的研究[J].中国中药杂志, 2011 , 36 (4) : 462
[4] 曹智, 张燕娣, 许永华, 等. 人参有效成分及其药理作用研究新进展[J]. 人参研究, 2012 , 2 : 39
[5] 何道同, 王兵, 陈珺明. 人参皂苷药理作用研究进展[J]. 辽宁中医药大学学报, 2012 , 14 (7) : 118
[6] 赵远,张凡,曲胜军,等 . 人参主要成分药理研究进展[J] . 亚太传统医药, 2012 , 8 (11) : 171
[7] 王常顺,刘永利,王晓蕾,等.UPLC、HPLC测定血栓通注射液中5个皂苷类成分含量的比较研究[J]. 药物分析杂志, 2013 , 33 (9) : 1617
[8] 曹树萍,聂黎行,王钢力,等. UPLC法用于参麦注射液及其中间体的皂苷含量研究[J]. 药物分析杂志, 2014 , 34 (7) : 1264
[9] 逄世峰, 孙成贺, 李亚丽, 等. UPLC法测定西洋参中7种人参皂苷含量[J] . 人参研究, 2013 , 33 (9) : 1617
[10] 张晓旭, 马雪涛,胡蒙, 等 .超高效液相色谱法检测6种人参皂苷含量[J]. 中国食品学报, 2015 , 15 (5) : 241
[2] 徐绥绪, 王乃利,李英辉. 中国红参化学成分的研究(Ⅱ)[J]. 药学学报, 1986 , 21(5) : 356
[3] 刘丹,濮社班,钱士辉, 等.中国红参化学成分的研究[J].中国中药杂志, 2011 , 36 (4) : 462
[4] 曹智, 张燕娣, 许永华, 等. 人参有效成分及其药理作用研究新进展[J]. 人参研究, 2012 , 2 : 39
[5] 何道同, 王兵, 陈珺明. 人参皂苷药理作用研究进展[J]. 辽宁中医药大学学报, 2012 , 14 (7) : 118
[6] 赵远,张凡,曲胜军,等 . 人参主要成分药理研究进展[J] . 亚太传统医药, 2012 , 8 (11) : 171
[7] 王常顺,刘永利,王晓蕾,等.UPLC、HPLC测定血栓通注射液中5个皂苷类成分含量的比较研究[J]. 药物分析杂志, 2013 , 33 (9) : 1617
[8] 曹树萍,聂黎行,王钢力,等. UPLC法用于参麦注射液及其中间体的皂苷含量研究[J]. 药物分析杂志, 2014 , 34 (7) : 1264
[9] 逄世峰, 孙成贺, 李亚丽, 等. UPLC法测定西洋参中7种人参皂苷含量[J] . 人参研究, 2013 , 33 (9) : 1617
[10] 张晓旭, 马雪涛,胡蒙, 等 .超高效液相色谱法检测6种人参皂苷含量[J]. 中国食品学报, 2015 , 15 (5) : 241