一株植物乳杆菌的粘附能力及免疫调节作用研究
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摘要
从西藏地区传统乳制品中分离得到的4株乳杆菌中筛选出一株粘附能力较强的植物乳杆菌TD109,选取不同剂量的菌悬液给小鼠灌服,于30 d后测定相关的免疫指标。实验结果表明:灌服植物乳杆菌TD109后,小鼠的胸腺指数由对照组的2.46提高到了高剂量组的2.93,脾脏指数由对照组的2.84提高到高剂量组的3.54;IL-2含量由对照组的126.01 ng/mL提高到高剂量组的295.08ng/mL、IFN-γ水平由对照组的122.66 ng/mL提高到高剂量组的149.81 ng/mL,TNF-α质量浓度由对照组的113.30 ng/mL降低到高剂量组的72.96ng/mL;同时MTT实验的结果也从对照组的0.994提高到高剂量组的1.868。初步证明了植物乳杆菌TD109对机体免疫能力有增强的作用。
Lactobacillus plantarum TD109 with a strong ability of adhesion was isolated from 4 Lactobacillus strains obtained from traditional dairy products in Tibet area. Mice were fed with different doses of bacterial suspension for 30 days. Related immune indexes were subsequently determined. The results showed that after consuming Lactobacillus plantarum TD109, the thymus index by 2.46 in the control group increased to 2.93 in the high-dose group, the spleen index raised from 2.84 of the control group increased to 3.54 of the high-dose group. IL-2 content increased from 126.01 ng/mL of the control group to 295.08 ng/mL of the high-dose group, IFN-γ levels went up to 149.81 ng/mL in the high-dose group from the control group of 122.66 ng/mL, the content of TNF-α decreased from 113.30 ng/mL of the control group to 72.96 ng/mL of the high-dose group. The data of MTT experiment was increased to 1.868 of high-dose group from 0.994 in the control group. It demonstrated that Lactobacillus plantarum TD109 had the ability of enhancing immunity.
Lactobacillus plantarum TD109 with a strong ability of adhesion was isolated from 4 Lactobacillus strains obtained from traditional dairy products in Tibet area. Mice were fed with different doses of bacterial suspension for 30 days. Related immune indexes were subsequently determined. The results showed that after consuming Lactobacillus plantarum TD109, the thymus index by 2.46 in the control group increased to 2.93 in the high-dose group, the spleen index raised from 2.84 of the control group increased to 3.54 of the high-dose group. IL-2 content increased from 126.01 ng/mL of the control group to 295.08 ng/mL of the high-dose group, IFN-γ levels went up to 149.81 ng/mL in the high-dose group from the control group of 122.66 ng/mL, the content of TNF-α decreased from 113.30 ng/mL of the control group to 72.96 ng/mL of the high-dose group. The data of MTT experiment was increased to 1.868 of high-dose group from 0.994 in the control group. It demonstrated that Lactobacillus plantarum TD109 had the ability of enhancing immunity.
引文
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[15]付彦君,张效禹.昆布提取物对小鼠免疫功能及抗疲劳能力的影响[J].天津中医药大学学报,2013,32(4):214-216.
[16]GU W Z,SU W H,ZHOU L X,et al.Two-way immune Therapy-unionapplication of immune suppression and immune modulator[J].Aerospace Medicine,2004,15(1):52-53.
[2]BUCK B L,ALTERMANN E,SVINGERUD T,et al.Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM[J].Appl Environ Microbiol,2005,71(12):8344-8351.
[3]汪海峰,章文明,汪以真,等.乳酸杆菌与肠道黏附相关表面因子及其机制的研究进展[J].动物营养学报,2011,23(2):179-186.
[4]WOHLGEMUTH S,LOH G,BLAUT M.Recent developments and perspectives in the investigation of probiotic effects[J].International Journal of Medical Microbiology,2010,300(1):3-10.
[5]OELSCHLAEGER T A.Mechanisms of probiotic actions-a review[J].International Journal of Medical Microbiology,2010,300(1):57-62.
[6]TSAI Y T,CHENG P C,PAN T M.The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits[J].Applied Microbiology and Biotechnology,2012,96(4):853-862.
[7]DELCENSERIE V,MARTEL D,LAMOUREUX M,et al.Immunomodulatory effects of probiotics in the intestinal tract[J].Current Issues in Molecular Biology,2008,10(1/2):37.
[8]中华人民共和国卫生部.保健食品检验与评价技术规范[J].中华人民共和国卫生部,2003:22-34.
[9]郭彤,胥保华,马玉龙,等.嗜酸乳杆菌和两歧双歧杆菌体外黏附Caco-2细胞及其对病原菌黏附性能的影响[J].中国兽医学报,2008,28(5):527-531.
[10]白洁,李卫芬,黄琴,等.几株益生乳酸菌对Caco-2细胞的黏附及其对致病菌黏附的影响[J].动物营养学报.2012,24(10):1992-1998.
[11]王红娟,郑峥,杨洁.新疆疆岳驴乳粉增强小鼠免疫功能的研究[J].中国乳品工业,2012,40(5):20-23.
[12]SOLTAN DALLAL M M,YAZDI M H,HOLAKUYEE M,et al.Lactobacillus casei ssp.casei induced Th1 cytokine profile and natural killer cells activity in invasive ductal carcinoma bearing mice[J].Iranian Journal of Allergy,Asthma,and Immunology,2012,11(2):183-189.
[13]YOON H S,JU J H,LEE J E,et al.The probiotic Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 promote cholesterol efflux and suppress inflammation in THP-1 Cells[J].Journal of the Science of Food and Agriculture,2013,93(4):781-787.
[14]刘少敏,满朝新,李理,姜毓君.乳酸菌免疫调节作用的研究进展[J].中国食物与营养,2013,19(4):60-63.
[15]付彦君,张效禹.昆布提取物对小鼠免疫功能及抗疲劳能力的影响[J].天津中医药大学学报,2013,32(4):214-216.
[16]GU W Z,SU W H,ZHOU L X,et al.Two-way immune Therapy-unionapplication of immune suppression and immune modulator[J].Aerospace Medicine,2004,15(1):52-53.