响应面法优化分子蒸馏提纯烟梗浸膏的工艺
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摘要
为了提高烟梗浸膏的利用价值,以超临界流体萃取法制备的烟梗浸膏为研究对象,采用响应面法对其分子蒸馏法分离提纯的工艺参数进行了优化,并对所得的精油成分进行了气相色谱质谱联用(GC/MS)检测。结果表明:①在蒸馏温度140℃,刮膜转速246 r/min,冷凝口温度39℃的条件下,分子蒸馏的效果较好,精油得率为10.49%±0.05%。②与烟梗浸膏相比,所得精油中有24种成分的质量分数得到大幅提高,其中桉叶油醇的富集倍数最高(112倍)。③所得精油应用于再造烟叶加香,能够有效提高感官品质。该方法适用于提纯烟梗浸膏,且所得精油可以增强产品的烟香。
To improve the utilization value of tobacco stem extract, molecular distillation was used to separate and purify tobacco stem extract prepared by supercritical fluid extraction. The parameters of molecular distillation were optimized by the response surface method, and the components in the extracted essential oil were determined by GC/MS. The results showed that: 1) The highest yield of the essential oil was 10.49% ±0.05%, the optimized processing parameters were distillation temperature 140 ℃, film scraping speed 246 r/min,and condensing temperature 39 ℃. 2) Comparing with tobacco stem extract, the contents(mass fraction) of 24 components in the obtained essential oil increased greatly, and the enrichment of eucalyptol was the highest and reached 112 times. 3) The obtained essential oil was used as a flavor for reconstituted tobacco, and the sensory evaluation results indicated positive sensory quality. This method is suitable for purifying tobacco stem extracts,and the obtained essential oil enhances the tobacco-like aroma of reconstituted tobacco products.
To improve the utilization value of tobacco stem extract, molecular distillation was used to separate and purify tobacco stem extract prepared by supercritical fluid extraction. The parameters of molecular distillation were optimized by the response surface method, and the components in the extracted essential oil were determined by GC/MS. The results showed that: 1) The highest yield of the essential oil was 10.49% ±0.05%, the optimized processing parameters were distillation temperature 140 ℃, film scraping speed 246 r/min,and condensing temperature 39 ℃. 2) Comparing with tobacco stem extract, the contents(mass fraction) of 24 components in the obtained essential oil increased greatly, and the enrichment of eucalyptol was the highest and reached 112 times. 3) The obtained essential oil was used as a flavor for reconstituted tobacco, and the sensory evaluation results indicated positive sensory quality. This method is suitable for purifying tobacco stem extracts,and the obtained essential oil enhances the tobacco-like aroma of reconstituted tobacco products.
引文
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[16]YC/T 498-2014再造烟叶(造纸法)感官评价方法[S].YC/T 498-2014 The evaluation methods of sensory quality for reconstituted tobacco(paper-process)[S].
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[18]康明娥,王光雨.新植二烯的合成研究[J].香料香精化妆品,2016(4):1-3.KANG Minge,WANG Guangyu.Study on the synthesis of neophytadiene[J].Flavour Fragrance Cosmetics,2016(4):1-3.
[2]于建军,魏登辉,田斌强,等.响应面法优化漆酶降解梗丝木质素的工艺[J].烟草科技,2017,50(3):57-64.YU Jianjun,WEI Denghui,TIAN Binqiang,et al.Optimization of lignin degradation by laccase in cut stem with response surface method[J].Tobacco Science&Technology,2017,50(3):57-64.
[3]杨菁,沈晓洁,张怡春,等.烟梗的超临界CO2流体萃取工艺及萃取物在电子烟中的应用[J].烟草科技,2016,49(7):51-59.YANG Jing,SHEN Xiaojie,ZHANG Yichun,et al.Supercritical CO2extraction on tobacco stem and application of stem extract in e-cigarette liquids[J].Tobacco Science&Technology,2016,49(7):51-59.
[4]高宏建,张献忠,钟建军,等.水蒸汽蒸馏法提取烟草精油的研究[J].食品工业科技,2011,32(10):388-390.GAO Hongjian,ZHANG Xianzhong,ZHONG Jianjun,et al.Research on extraction tobacco essential oils by steam distillation[J].Science and Technology of Food Industry,2011,32(10):388-390.
[5]杨君,高宏建,王宇,等.超声辅助溶剂法提取烟草精油粗提物的研究[J].中国食品学报,2012,12(4):65-71.YANG Jun,GAO Hongjian,WANG Yu,et al.Research of extraction tobacco essential oils by ultrasonicassisted method[J].Journal of Chinese Institute of Food Science and Technology,2012,12(4):65-71.
[6]刘亚娟,刘芸,王志祥.超临界CO2萃取烟草精油的工艺研究[J].化学与生物工程,2014,31(6):59-63.LIU Yajuan,LIU Yun,WANG Zhixiang.Study on the extraction process of essential oil from tobacco by supercritical CO2fluid[J].Chemistry&Bioengineering,2014,31(6):59-63.
[7]瞿先中,王宏伟,程雷平.超临界萃取烟草精油条件的优化[J].安徽农业科学,2010,38(35):20448-20450.QU Xianzhong,WANG Hongwei,CHENG Leiping.Optimization of supercritical extraction conditions of essential oil from tobacco[J].Journal of Anhui Agricultural Sciences,2010,38(35):20448-20450.
[8]谭天伟.天然产物分离新技术[J].化工进展,2003,22(7):665-668.Tan Tianwei.New separation and purification methods for natural herbs[J].Chemical Industry and Engineering Progress,2003,22(7):665-668.
[9]邢红丽.分子蒸馏技术的应用与发展[J].武汉工业学院学报,2005,24(1):65-67,71.XING Hongli.Application and development of molecular distillation technology[J].Journal of Wuhan Polytechnic University,2005,24(1):65-67,71.
[10]冯武文,杨村,于宏奇,等.分子蒸馏技术--一项特殊的液-液分离技术[J].上海化工,1999(3/4):38-39.FENG Wuwen,YANG Cun,YU Hongqi,et al.Molecular distillation-a special liquid-liquid separation technique[J].Shanghai Chemical Industry,1999(3/4):38-39.
[11]Micov M,Luti?an J,Cvengro?J.Balance equations for molecular distillation[J].Separation Science and Technology,1997,32(18):3051-3066.
[12]刘华,葛发欢.分子蒸馏新技术在天然产物分离和其它领域中的应用[J].中药材,1999,22(3):152-156.LIU Hua,GE Fahuan.Application of molecular distillation technology in natural product separation and other fields[J].Chinese Medicinal Materials,1999,22(3):152-156.
[13]许松林,徐世民,干爱华.天然产物分离的新技术--分子蒸馏[J].中草药,2001,32(6):562-563.XU Songlin,XU Shimin,GAN Aihua.Application of molecular distillation on separation of natural products[J].Chinese Traditional and Herbal Drugs,2001,32(6):562-563.
[14]易聪华,冀唯妮,孔浩辉,等.烟梗本香物质的超临界CO2萃取[J].华南理工大学学报(自然科学版),2017,45(6):103-108.YI Conghua,JI Weini,KONG Haohui,et al.Supercritical CO2fluid extraction of natural flavor components in tobacco stem[J].Journal of South China University of Technology(Natural Science),2017,45(6):103-108.
[15]易聪华,张素文,冀唯妮,等.氧碱法脱除烟梗中木质素降低烟气有害成分[J].华南理工大学学报(自然科学版),2016,44(6):21-26.YI Conghua,ZHANG Suwen,JI Weini,et al.Reduction of harmful components in cigarette smoke via delignification of tobacco stem by means of oxygen alkali technology[J].Journal of South China University of Technology(Natural Science),2016,44(6):21-26.
[16]YC/T 498-2014再造烟叶(造纸法)感官评价方法[S].YC/T 498-2014 The evaluation methods of sensory quality for reconstituted tobacco(paper-process)[S].
[17]黄申,夏璠,钱玉梅,等.西柏三烯-4,6-二醇降解产香菌培养基条件优化[J].烟草科技,2017,50(12):55-60,69.HUANG Shen,XIA Fan,QIAN Yumei,et al.Optimization of medium for cembratriene-4,6-diol degradation bacteria[J].Tobacco Science&echnology,2017,50(12):55-60,69.
[18]康明娥,王光雨.新植二烯的合成研究[J].香料香精化妆品,2016(4):1-3.KANG Minge,WANG Guangyu.Study on the synthesis of neophytadiene[J].Flavour Fragrance Cosmetics,2016(4):1-3.