改性壳聚糖抗菌纳米微球的制备
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摘要
以卤胺化合物为抗菌基团对壳聚糖接枝改性,并制备成纳米微球,提高壳聚糖的抗菌性能.通过核磁共振和紫外光谱对改性壳聚糖进行结构表征;探讨了改性壳聚糖浓度、三聚磷酸钠浓度及两者体积比对纳米微球的形成和粒径分布的影响;测定了纳米微球的抗菌性能.结果表明,在改性壳聚糖浓度为4. 0 mg/m L,三聚磷酸钠浓度为2. 0 mg/m L时,形成的纳米微球形态稳定,粒径分布均匀,氯化后的纳米微球可在30 min内杀灭10~7cfu(cfu为单位体积中的菌落总数)的金黄色葡萄球菌和大肠杆菌,表现出优异的抗菌性能.
A novel antibacterial nanospheres was prepared by modification of chitosan with N-halamine,and characterized by nuclear magnetic resonance( NMR) and ultraviolet spectrum( UV). The effect of concentration and volume ratio of CS-HDH,polysodium tripolyphosphate( TPP) and the volume ratio of the two polymers on the formation and particle size of nanoparticles were discussed. The antibacterial properties were also measured. The results show that the nanospheres has a stable shape and uniform distribution of particle size. Excellent antibacterial properties can be obtained,and 10~7cfu( colong-forming units) of S. aureus and E. coli can be killed within 30 min.
A novel antibacterial nanospheres was prepared by modification of chitosan with N-halamine,and characterized by nuclear magnetic resonance( NMR) and ultraviolet spectrum( UV). The effect of concentration and volume ratio of CS-HDH,polysodium tripolyphosphate( TPP) and the volume ratio of the two polymers on the formation and particle size of nanoparticles were discussed. The antibacterial properties were also measured. The results show that the nanospheres has a stable shape and uniform distribution of particle size. Excellent antibacterial properties can be obtained,and 10~7cfu( colong-forming units) of S. aureus and E. coli can be killed within 30 min.
引文
[1] Wang A. Q.,Chitin Chemistry,Science Press,Beijing,2007(王爱勤.甲壳素化学.北京:科学出版社,2007)
[2] Guo M. M.,Study on Applications of Chitosan in Antimicrobial Food Packaging,Jiangnan University,Wuxi,2015(郭鸣鸣.壳聚糖在几种食品抗菌包装中的应用研究.无锡:江南大学,2015)
[3] Cruz-Romero M. C.,Food Control,2013,34,393—397
[4] Sui S. Y.,Study on Whey Protein Concentrate-chitosan Antibacterial Composite Edible Films,Jilin University,Changchun,2015(隋思瑶.浓缩乳清蛋白-壳聚糖抗菌复合膜的研究.长春:吉林大学,2015)
[5] Williams D. E.,Elder E. D.,Worley S. D.,Applied and Environmental Microbiology,1988,54(10),2583—2585
[6] Liu Y.,Liu Y.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,296,231—236
[7] Jiang Z. M.,Ma K. K.,Du J. M.,Li R.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,288,518—523
[8] Li R.,Hu P.,Ren X. H.,Worley S. D.,Huang T. S.,Carbohydrate Polymers,2013,92,534—539
[9] Cheng X. L.,Ma K. K.,Li R.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,309,138—143
[10] Yin M. L.,Chen X. L.,Li R.,Huang D.,Fan X. Y.,Ren X. H.,Huang T. S.,Journal of Applied Polymer Science,2016,133(46),https://doi.org/10.1002/app.44204
[11] Wang Y. F.,Li L.,Liu Y.,Ren X. H.,Liang J.,Materials Science and Engineering C,2016,69,1075—1080
[12] Wu Z. Y.,Chen Z.,Huang H.,Tian P. P.,Journal of Nantong University(Natural Science Edition),2005,25,20—22(吴振宇,陈钟,黄华,田鹏鹏.南通大学学报(自然科学版),2005,25,20—22)
[13] Gierszewska-Dru6)zyn'ska M.,Ostrowska-Czubenko J.,Polish Chitin Society,2011,16,15—22
[14] Qi L. F.,Xu Z. R.,Jiang X.,Hu C. H.,Zou X. F.,Carbohydrate Research,2004,339,2693—2700
[15] Wang Y. F.,Liu Y.,Tian H. R.,Zhai Y. S.,Pan N. Y.,Yin M. L.,Ren X. H.,Liang J.,Colloid and Polymer Science,2017,295(910),1897—1900
[2] Guo M. M.,Study on Applications of Chitosan in Antimicrobial Food Packaging,Jiangnan University,Wuxi,2015(郭鸣鸣.壳聚糖在几种食品抗菌包装中的应用研究.无锡:江南大学,2015)
[3] Cruz-Romero M. C.,Food Control,2013,34,393—397
[4] Sui S. Y.,Study on Whey Protein Concentrate-chitosan Antibacterial Composite Edible Films,Jilin University,Changchun,2015(隋思瑶.浓缩乳清蛋白-壳聚糖抗菌复合膜的研究.长春:吉林大学,2015)
[5] Williams D. E.,Elder E. D.,Worley S. D.,Applied and Environmental Microbiology,1988,54(10),2583—2585
[6] Liu Y.,Liu Y.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,296,231—236
[7] Jiang Z. M.,Ma K. K.,Du J. M.,Li R.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,288,518—523
[8] Li R.,Hu P.,Ren X. H.,Worley S. D.,Huang T. S.,Carbohydrate Polymers,2013,92,534—539
[9] Cheng X. L.,Ma K. K.,Li R.,Ren X. H.,Huang T. S.,Applied Surface Science,2014,309,138—143
[10] Yin M. L.,Chen X. L.,Li R.,Huang D.,Fan X. Y.,Ren X. H.,Huang T. S.,Journal of Applied Polymer Science,2016,133(46),https://doi.org/10.1002/app.44204
[11] Wang Y. F.,Li L.,Liu Y.,Ren X. H.,Liang J.,Materials Science and Engineering C,2016,69,1075—1080
[12] Wu Z. Y.,Chen Z.,Huang H.,Tian P. P.,Journal of Nantong University(Natural Science Edition),2005,25,20—22(吴振宇,陈钟,黄华,田鹏鹏.南通大学学报(自然科学版),2005,25,20—22)
[13] Gierszewska-Dru6)zyn'ska M.,Ostrowska-Czubenko J.,Polish Chitin Society,2011,16,15—22
[14] Qi L. F.,Xu Z. R.,Jiang X.,Hu C. H.,Zou X. F.,Carbohydrate Research,2004,339,2693—2700
[15] Wang Y. F.,Liu Y.,Tian H. R.,Zhai Y. S.,Pan N. Y.,Yin M. L.,Ren X. H.,Liang J.,Colloid and Polymer Science,2017,295(910),1897—1900