新型饲草串叶松香草SOD提取条件优化及其抗氧化能力
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摘要
本文旨在对不同生长期串叶松香草超氧化物歧化酶(SOD)提取条件进行优化,并对其提取液中类SOD活性物质及抗氧化能力进行考察。采用单因素和正交试验方法,以SOD活力为标准,优化提取条件,并测定串叶松香草不同生长阶段总黄酮、多酚类化合物和维生素C等类SOD活性物质含量,同时对不同生长阶段提取液中羟自由基、1,1-二苯代苦味酞基(DPPH)自由基及超氧阴离子的清除能力进行测定。结果表明:叶丛期与开花期的水提条件为料液比1∶25,37℃水浴浸提12 h,其提取液SOD活性分别为4030.12、4445.31 U/g。与叶丛期相比,开花期维生素C、总黄酮和总多酚含量分别提高34.51%(P <0.01)、56.59%(P <0.01)和23.15%(P <0.01),开花期提取液清除羟自由基和DPPH的能力分别提高205.65%(P <0.01)和12.20%(P <0.01)。综上,串叶松香草开花期比叶丛期抗氧化能力更强。
This study was to investigate best condition for extraction superoxide disinutase(SOD) from different growth phase of Silphium perfoliatum and evaluate the effects of SOD like activity substances and antioxidant activity of extract from Silphium perfoliatum.Use SOD activity as the standard to optimize extraction condition by the single factor experiments and orthogonal experiments.The content of SOD-simultaneous active substances of Silphium perfoliatum were determided. Determination of flavonoids,polyphenols and vitamin C(VC) content by standard curve method.Meanwhile,put the liquid antioxidant capacity to take preliminary determination,including its hydroxyl radical·OH,DPPH and superoxide anion scavenging capacity in different growing stage.The results showed that in the condition that solid-liquid ratio 1: 25,extaction temperature 37 ℃,extraction time 12 h,the SOD in Silphium perfoliatum water extract of the highest activity(4030.12 U/g and 4445.31 U/g) in the leaf stage and flowering stage.Compared with the leaf stage,the contents of VC,total flavonoid and total polyphenols in water extracts of Silphium perfoliatum were significantly increased by 34.51%(P < 0.01),56.59%(P < 0.01) and 23.15%(P < 0.01).Meanwhile, the ability of hydroxyl radicals and DPPH scavenging in water extracts of Silphium perfoliatum at the flowering stage were increased by 205.65%(P <0.01) and 12.20%(P < 0.01) than the leaf stage.
This study was to investigate best condition for extraction superoxide disinutase(SOD) from different growth phase of Silphium perfoliatum and evaluate the effects of SOD like activity substances and antioxidant activity of extract from Silphium perfoliatum.Use SOD activity as the standard to optimize extraction condition by the single factor experiments and orthogonal experiments.The content of SOD-simultaneous active substances of Silphium perfoliatum were determided. Determination of flavonoids,polyphenols and vitamin C(VC) content by standard curve method.Meanwhile,put the liquid antioxidant capacity to take preliminary determination,including its hydroxyl radical·OH,DPPH and superoxide anion scavenging capacity in different growing stage.The results showed that in the condition that solid-liquid ratio 1: 25,extaction temperature 37 ℃,extraction time 12 h,the SOD in Silphium perfoliatum water extract of the highest activity(4030.12 U/g and 4445.31 U/g) in the leaf stage and flowering stage.Compared with the leaf stage,the contents of VC,total flavonoid and total polyphenols in water extracts of Silphium perfoliatum were significantly increased by 34.51%(P < 0.01),56.59%(P < 0.01) and 23.15%(P < 0.01).Meanwhile, the ability of hydroxyl radicals and DPPH scavenging in water extracts of Silphium perfoliatum at the flowering stage were increased by 205.65%(P <0.01) and 12.20%(P < 0.01) than the leaf stage.
引文
[1]刘军军,刘金兰,李帅,等.拳参黄酮与VC、芦丁协同抗氧化作用研究[J].化学与生物工程.2014,31(6):34~37.
[2]刘生龙,王成信,王祺.优良饲用植物串叶松香草引种试验[J].草业科学,1995,12(2):29~31.
[3]刘文海.串叶松香草的新用途_提取SOD开发叶蛋白[J].现代生物医学进展,2008,8(11):2163~2165.
[4]娜日苏.优良青饲料引种栽培及应用研究:[硕士学位论文][D].内蒙古:内蒙古农业大学,2006.
[5]聂芙蓉,刘太宇,李梦云,等.串叶松香不同生育期营养成分及其绵羊瘤胃降解动态研究[J].西北农林科技大学学报,2009,37(5):69~73.
[6]谭兴和,甘霖,秦丹,等.串叶松香草营养成分及其营养价值分析[J].保鲜与加工.2003,3(2):10~12.
[7]王玮,杨桂英.优质高产饲料作物——串叶松香草的引种栽培试验[J].饲料与畜牧,2004,4:32~33.
[8]徐善光,周威,王书凯,等.浅谈串叶松香草的应用价值及栽培要点[J].辽宁林业科技.2010,1:61~62.
[9]杨楠,贾晓斌,张振海,等.黄酮类化合物抗肿瘤活性及机制研究进展[J].中国中药杂志,2015,40(3):373~384.
[10]张薇.串叶松香草类SOD研究:[硕士学位论文][D].上海:华东理工大学,2012.
[11]赵明坤,韩永芬.串叶松香草添加不同比例精料舍饲育肥山羊试验[J].贵州畜牧兽医.2001,25(4):6.
[12] Almeselmani M,Deshmukh P S,Sairam R K,et al.Protective role of antioxidant enzymes under high temperature stress[J].Plant Science.2006,171(3):382
[13] Hong Mei Shang,Hai Zhu Zhou,Ran Li,et al.Extraction optimization and influences of drying methods on antioxidant activities of polysaccharide from cup plant(Silphium perfoliatum L.)[J].Pols One.2017,12(8):e0183001.
[14] K owalski R,Kedziab B.Evaluation of antibacterial activity of Silphium perfoliatum extracts[J].Pharm Biology.2007,6:494~500.
[15] Yergaliyev T M,Nurbekova Z,Mukiyanova G,et al.The involvement of ROS producing aldehyde oxidase in plant response to Tombusvirus infection[J].Plant Physiology Biochemistry,2016,109:36~44.
[2]刘生龙,王成信,王祺.优良饲用植物串叶松香草引种试验[J].草业科学,1995,12(2):29~31.
[3]刘文海.串叶松香草的新用途_提取SOD开发叶蛋白[J].现代生物医学进展,2008,8(11):2163~2165.
[4]娜日苏.优良青饲料引种栽培及应用研究:[硕士学位论文][D].内蒙古:内蒙古农业大学,2006.
[5]聂芙蓉,刘太宇,李梦云,等.串叶松香不同生育期营养成分及其绵羊瘤胃降解动态研究[J].西北农林科技大学学报,2009,37(5):69~73.
[6]谭兴和,甘霖,秦丹,等.串叶松香草营养成分及其营养价值分析[J].保鲜与加工.2003,3(2):10~12.
[7]王玮,杨桂英.优质高产饲料作物——串叶松香草的引种栽培试验[J].饲料与畜牧,2004,4:32~33.
[8]徐善光,周威,王书凯,等.浅谈串叶松香草的应用价值及栽培要点[J].辽宁林业科技.2010,1:61~62.
[9]杨楠,贾晓斌,张振海,等.黄酮类化合物抗肿瘤活性及机制研究进展[J].中国中药杂志,2015,40(3):373~384.
[10]张薇.串叶松香草类SOD研究:[硕士学位论文][D].上海:华东理工大学,2012.
[11]赵明坤,韩永芬.串叶松香草添加不同比例精料舍饲育肥山羊试验[J].贵州畜牧兽医.2001,25(4):6.
[12] Almeselmani M,Deshmukh P S,Sairam R K,et al.Protective role of antioxidant enzymes under high temperature stress[J].Plant Science.2006,171(3):382
[13] Hong Mei Shang,Hai Zhu Zhou,Ran Li,et al.Extraction optimization and influences of drying methods on antioxidant activities of polysaccharide from cup plant(Silphium perfoliatum L.)[J].Pols One.2017,12(8):e0183001.
[14] K owalski R,Kedziab B.Evaluation of antibacterial activity of Silphium perfoliatum extracts[J].Pharm Biology.2007,6:494~500.
[15] Yergaliyev T M,Nurbekova Z,Mukiyanova G,et al.The involvement of ROS producing aldehyde oxidase in plant response to Tombusvirus infection[J].Plant Physiology Biochemistry,2016,109:36~44.