不同来源胡敏酸化学组成及其结合态磷素的生物有效性
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摘要
磷(P)是存在于腐殖物质中的主要元素之一,然而不同来源腐殖物质中P素的生物有效性及其影响因素尚不清楚.本研究以土壤、泥炭、玉米秸秆、牛粪、猪粪和鸡粪为供试材料,利用酶水解方法比较了不同来源胡敏酸(HA)中P素生物有效性的差异,分析了P素生物有效性与HA化学组成之间的关系.结果发现:HA中活性无机P占HA全P的19.9%～46.4%,活性有机P占HA全P的23.3%～53.8%,活性P总量占HA全P的43.3%～84.7%;活性有机P中,类植酸态P的比例(9.13%～25.1%)最高,其次为简单单酯P(7.06%～22.3%)和多核苷酸P(4.12%～7.46%);不同来源HA相比,活性无机P的含量以土壤HA最高而鸡粪HA最低,活性有机P的含量以秸秆HA最高而牛粪HA最低,活性P总量的顺序为秸秆HA>土壤HA≈猪粪HA>泥炭HA>牛粪HA>鸡粪HA;Pearson线性相关分析指出,活性无机P与Al含量之间呈显著的负相关,活性有机P与Ca、Mg、Zn、碳水化合物和双烷氧碳含量之间呈显著的正相关,而活性P总量与Fe含量之间呈显著的负相关.上述结果说明,不同来源HA结合态P素的生物有效性不同,其中秸秆HA结合态P素的生物有效性最高,其次为土壤HA、猪粪HA、泥炭HA和牛粪HA,而鸡粪HA结合态P素的生物有效性最低;HA中酶可水解的活性有机P含量与其中金属元素和有机碳官能团的组成有关,HA中与Ca、Mg和Zn结合的P是酶可水解的有机P形态,同时HA中活性有机碳官能团含量较高则活性有机P的含量也较高.
Phosphorus(P) is one of the major elements in humic substances. However, the bioavailability and influencing factors of P associated with humic substances from different sources are still unclear. In this study, humic acids(HA) were isolated from soil, peat, maize straw, and livestock manures(i.e., cattle manure, pig manure, and chicken manure). The differences of the bioavailability of P associated HA were compared by using enzymatic hydrolysis experiments. Moreover, the correlations between P bioavailability and chemical compositions of HA were explored. The labile inorganic P, labile organic P, and total labile P accounted for 19.9%～46.4%, 23.3%～53.8%, and 43.3%～84.7% of total P in HA, respectively. In the labile organic P, phytate-like P displayed the highest proportion(9.13%～25.1%), followed by simple monoester P(7.06%～22.3%) and polynucleotide P(4.12%～7.46%). Among HA from the different sources, the labile inorganic P content was highest in HA from soil and lowest in HA from chicken manure, while the labile organic P content was highest in HA from maize straw and lowest in HA from cattle manure. The total labile P content in HA from different sources was in the order maize straw > soil ≈ pig manure > peat > cattle manure > chicken manure. Pearson correlation analysis shows that the labile inorganic P was negatively correlated with Al content, the labile organic P was positively correlated with Ca, Mg, Zn, carbohydrate C, and di-O-alkyl C, whereas the total labile P was negatively correlated with Fe content in HA. The above results suggest that the bioavailability of P associated with HA from different sources was distinct, in which the bioavailability of P is the highest for HA from maize straw, followed by HA from soil, pig manure, peat and cattle manure, and the lowest is for HA from chicken manure. The Ca-, Mg-, and Zn-bound P in HA are enzyme hydrolysable organic P forms, and the labile organic P is high in HA with high labile organic C functional groups.
Phosphorus(P) is one of the major elements in humic substances. However, the bioavailability and influencing factors of P associated with humic substances from different sources are still unclear. In this study, humic acids(HA) were isolated from soil, peat, maize straw, and livestock manures(i.e., cattle manure, pig manure, and chicken manure). The differences of the bioavailability of P associated HA were compared by using enzymatic hydrolysis experiments. Moreover, the correlations between P bioavailability and chemical compositions of HA were explored. The labile inorganic P, labile organic P, and total labile P accounted for 19.9%～46.4%, 23.3%～53.8%, and 43.3%～84.7% of total P in HA, respectively. In the labile organic P, phytate-like P displayed the highest proportion(9.13%～25.1%), followed by simple monoester P(7.06%～22.3%) and polynucleotide P(4.12%～7.46%). Among HA from the different sources, the labile inorganic P content was highest in HA from soil and lowest in HA from chicken manure, while the labile organic P content was highest in HA from maize straw and lowest in HA from cattle manure. The total labile P content in HA from different sources was in the order maize straw > soil ≈ pig manure > peat > cattle manure > chicken manure. Pearson correlation analysis shows that the labile inorganic P was negatively correlated with Al content, the labile organic P was positively correlated with Ca, Mg, Zn, carbohydrate C, and di-O-alkyl C, whereas the total labile P was negatively correlated with Fe content in HA. The above results suggest that the bioavailability of P associated with HA from different sources was distinct, in which the bioavailability of P is the highest for HA from maize straw, followed by HA from soil, pig manure, peat and cattle manure, and the lowest is for HA from chicken manure. The Ca-, Mg-, and Zn-bound P in HA are enzyme hydrolysable organic P forms, and the labile organic P is high in HA with high labile organic C functional groups.
引文
Anderson K R,Moore Jr P A,Miller D M,et al.2018.Phosphorus leaching from soil cores from a twenty-year study evaluating alum treatment of poultry litter [J].Journal of Environmental Quality,47:530-537
ková G,Liptaj T,Prónayová N.2007.Phosphorus fractions in arable and mountain soils and their humic acids [J].Soil and Water Research,2(4):141-148
Bedrock C N,Cheshire M V,Shand C A.1997.The involvement of iron and aluminum in the bonding of phosphorus to soil humic acid [J].Communications in Soil Science and Plant Analysis,28(11/12):961-967
曹志宏,黄艳丽,郝晋珉.2018.中国作物秸秆资源利用潜力的多适宜性综合评价[J].环境科学研究,31(1):179-186
窦森.2010.土壤有机质[M].北京:科学出版社
Gerke J.2010.Humic (organic matter)-Al(Fe)-phosphate complexes:An underestimated phosphate form in soils and source of plant-available phosphate [J].Soil Science,175(9):417-425
Guan S,An N,Zong N,et al.2018.Climate warming impacts on soil organic carbon fractions and aggregate stability in a Tibetan alpine meadow [J].Soil Biology and Biochemistry,116:224-236
Haygarth P M,Hinsinger P,Blackburn D.2018.Organic phosphorus:potential solutions for phosphorus security [J].Plant and Soil,427:1-3
He Z,Honeycutt C W,Xing B,et al.2007.Solid-state Fourier transform infrared and 31P nuclear magnetic resonance spectral features of phosphate compounds [J].Soil Science,172(7):501-515
He Z,Honeycutt C W.2005.A modified molybdate blue method for orthophosphate determination suitable for investigating enzymatic hydrolysis of organic phosphates [J].Communications in Soil Science and Plant Analysis,36(9/10):1373-1383
He Z,Ohno T,Cade-Menun B J,et al.2006.Spectral and chemical characterization of phosphates associated with humic substances [J].Soil Science Society of America Journal,70(5):1741-1751
He Z,Olk D C,Cade-Menun B J.2011.Forms and lability of phosphorus in humic acid fractions of Hord silt loam soil [J].Soil Science Society of America Journal,75(5):1712-1722
He Z,Olk D C,Honeycutt C W,et al.2009.Enzymatically and ultraviolet-labile phosphorus in humic acid fractions from rice soils [J].Soil Science,174(2):81-87
He Z,Pagliari P H,Waldrip H M.2016.Applied and environmental chemistry of animal manure:A review [J].Pedosphere,26(6):779-816
Huang L,Yang J,Xu Y,et al.2018.The contrasting effects of alum-treated chicken manures and KH2PO4 on phosphorus behavior in soils [J].Journal of Environmental Quality,47(2):345-352
黄绍文,唐继伟,李春花.2017.我国商品有机肥和有机废弃物中重金属、养分和盐分含量[J].植物营养与肥料学报,23(1):162-173
Kelleher B P,Simpson A J,Willeford K O,et al.2004.Acid phosphatase interactions with organo-mineral complexes:influence on catalytic activity [J].Biogeochemistry,71(3):285-297
Li B,Brett M T.2013.The influence of dissolved phosphorus molecular form on recalcitrance and bioavailability [J].Environmental Pollution,182:37-44
Li C,Ji F,Wang S,et al.2015.Adsorption of Cu(II) on humic acids derived from different organic materials [J].Journal of Integrative Agriculture,14(1):168-177
Li W,Joshi S R,Hou G,et al.2015.Characterizing phosphorus speciation of Chesapeake Bay sediments using chemical extraction,31P NMR,and X-ray absorption fine structure spectroscopy [J].Environmental Science and Technology,49(1):203-211
Linquist B A,Ruark M D,Hill J E.2011.Soil order and management practices control soil phosphorus fractions in managed wetland ecosystems [J].Nutrient Cycling in Agroecosystems,90(1):51-62
Liu S,Zhu Y,Wu F,et al.2017.Using solid 13C NMR coupled with solution 31P NMR spectroscopy to investigate molecular species and lability of organic carbon and phosphorus from aquatic plants in Tai Lake,China [J].Environmental Science and Pollution Research,24(2):1880-1889
Mahieu N,Olk D C,Randall E W.2002.Multinuclear magnetic resonance analysis of two humic acid fractions from lowland rice soils [J].Journal of Environmental Quality,31(2):421-430
McDowell R W,Condron L M,Mahieu N,et al.2002.Analysis of potentially mobile phosphorus in arable soils using solid state nuclear magnetic resonance [J].Journal of Environmental Quality,31(2):450-456
Menezes-Blackburn D,Giles C,Darch T,et al.2018.Opportunities for mobilizing recalcitrant phosphorus from agricultural soils:a review [J].Plant and Soil,427:5-16
Olaetxeaa M,De Hitaa D,Garcia C A,et al.2018.Hypothetical framework integrating the main mechanisms involved in the promoting action of rhizospheric humic substances on plant root- and shoot-growth [J].Applied Soil Ecology,123:521-537
Parent L-E,IInicki P.2002.Organic Soils and Peat Materials for Sustainable Agriculture [M].Boca Raton:CRC Press
Petrovic M,Kastelan-Macan M.1996.The uptake of inorganic phosphorus by insoluble metal-humic complexes [J].Water Science and Technology,34(7/8):253-258
Roberto B,Urrutia O,Erro J,et al.2016.Humic-(metal)-phosphate complexes characterization and ecological role in phosphorus bioavailability on plant nutrition [C].18th International Conference of International Humic Substances Society.Kanazawa,Japan
Romanyà J,Blanco-Moreno J M,Sans F X.2017.Phosphorus mobilization in low-P arable soils may involve soil organic C depletion [J].Soil Biology and Biochemistry,113:250-259
Senesi N,Plaza C,Brunetti G,et al.2007.A comparative survey of recent results on humic-like fractions in organic amendments and effects on native soil humic substances [J].Soil Biology and Biochemistry,39(6):1244-1262
苏德纯,张福锁,李国学.2000.磷-金属(Fe、Al)-有机酸三元复合体在植物磷营养中的作用[J].土壤通报,31(4):159-161
Stevenson F J.1994.Humus Chemistry:Genesis,Composition,Reactions,second ed [M].New York:Wiley & Sons
Turner B L,Frossard E,Baldwin D S.2005.Organic Phosphorus in the Environment [M].Wallingford:CABI Publishing
Urrutia O,Erro J,Guardado I,et al.2014.Physico-chemical characterization of humic-metal-phosphate complexes and their potential application to the manufacture of new types of phosphate-based fertilizers [J].Journal of Plant Nutrition and Soil Science,177(2):128-136
Wang J,Wu Y,Zhou J,et al.2016.Carbon demand drives microbial mineralization of organic phosphorus during the early stage of soil development [J].Biology and Fertility of Soils,52(6):825-839
王涛,周健民,王火焰.2011.固体废弃物及土壤中磷的形态分析技术[J].土壤学报,48(1):185-191
严玉鹏,万彪,刘凡,等.2012.环境中植酸的分布、形态及界面反应行为[J].应用与环境生物学报,18(3):494-501
袁露露.2018.中国泥炭地资源管理和利用标准体系建立研究[D].长春:东北师范大学
张友,宋佳伟,吕迎春,等.2017.荣成天鹅湖沉积物有机磷的生物有效性及其时空动态[J].环境科学学报,37(12):4629-4635
Zhu Y,Wu F,He Z,et al.2015.Influence of natural organic matter on the bioavailability and preservation of organic phosphorus in lake sediments [J].Chemical Geology,397:51-60
Zhu Y,Wu F,Liu S,et al.2018.Bioavailability and preservation of organic phosphorus in lake sediments:Insights from enzymatic hydrolysis and 31P nuclear magnetic resonance [J].Chemosphere,211:50-61
ková G,Liptaj T,Prónayová N.2007.Phosphorus fractions in arable and mountain soils and their humic acids [J].Soil and Water Research,2(4):141-148
Bedrock C N,Cheshire M V,Shand C A.1997.The involvement of iron and aluminum in the bonding of phosphorus to soil humic acid [J].Communications in Soil Science and Plant Analysis,28(11/12):961-967
曹志宏,黄艳丽,郝晋珉.2018.中国作物秸秆资源利用潜力的多适宜性综合评价[J].环境科学研究,31(1):179-186
窦森.2010.土壤有机质[M].北京:科学出版社
Gerke J.2010.Humic (organic matter)-Al(Fe)-phosphate complexes:An underestimated phosphate form in soils and source of plant-available phosphate [J].Soil Science,175(9):417-425
Guan S,An N,Zong N,et al.2018.Climate warming impacts on soil organic carbon fractions and aggregate stability in a Tibetan alpine meadow [J].Soil Biology and Biochemistry,116:224-236
Haygarth P M,Hinsinger P,Blackburn D.2018.Organic phosphorus:potential solutions for phosphorus security [J].Plant and Soil,427:1-3
He Z,Honeycutt C W,Xing B,et al.2007.Solid-state Fourier transform infrared and 31P nuclear magnetic resonance spectral features of phosphate compounds [J].Soil Science,172(7):501-515
He Z,Honeycutt C W.2005.A modified molybdate blue method for orthophosphate determination suitable for investigating enzymatic hydrolysis of organic phosphates [J].Communications in Soil Science and Plant Analysis,36(9/10):1373-1383
He Z,Ohno T,Cade-Menun B J,et al.2006.Spectral and chemical characterization of phosphates associated with humic substances [J].Soil Science Society of America Journal,70(5):1741-1751
He Z,Olk D C,Cade-Menun B J.2011.Forms and lability of phosphorus in humic acid fractions of Hord silt loam soil [J].Soil Science Society of America Journal,75(5):1712-1722
He Z,Olk D C,Honeycutt C W,et al.2009.Enzymatically and ultraviolet-labile phosphorus in humic acid fractions from rice soils [J].Soil Science,174(2):81-87
He Z,Pagliari P H,Waldrip H M.2016.Applied and environmental chemistry of animal manure:A review [J].Pedosphere,26(6):779-816
Huang L,Yang J,Xu Y,et al.2018.The contrasting effects of alum-treated chicken manures and KH2PO4 on phosphorus behavior in soils [J].Journal of Environmental Quality,47(2):345-352
黄绍文,唐继伟,李春花.2017.我国商品有机肥和有机废弃物中重金属、养分和盐分含量[J].植物营养与肥料学报,23(1):162-173
Kelleher B P,Simpson A J,Willeford K O,et al.2004.Acid phosphatase interactions with organo-mineral complexes:influence on catalytic activity [J].Biogeochemistry,71(3):285-297
Li B,Brett M T.2013.The influence of dissolved phosphorus molecular form on recalcitrance and bioavailability [J].Environmental Pollution,182:37-44
Li C,Ji F,Wang S,et al.2015.Adsorption of Cu(II) on humic acids derived from different organic materials [J].Journal of Integrative Agriculture,14(1):168-177
Li W,Joshi S R,Hou G,et al.2015.Characterizing phosphorus speciation of Chesapeake Bay sediments using chemical extraction,31P NMR,and X-ray absorption fine structure spectroscopy [J].Environmental Science and Technology,49(1):203-211
Linquist B A,Ruark M D,Hill J E.2011.Soil order and management practices control soil phosphorus fractions in managed wetland ecosystems [J].Nutrient Cycling in Agroecosystems,90(1):51-62
Liu S,Zhu Y,Wu F,et al.2017.Using solid 13C NMR coupled with solution 31P NMR spectroscopy to investigate molecular species and lability of organic carbon and phosphorus from aquatic plants in Tai Lake,China [J].Environmental Science and Pollution Research,24(2):1880-1889
Mahieu N,Olk D C,Randall E W.2002.Multinuclear magnetic resonance analysis of two humic acid fractions from lowland rice soils [J].Journal of Environmental Quality,31(2):421-430
McDowell R W,Condron L M,Mahieu N,et al.2002.Analysis of potentially mobile phosphorus in arable soils using solid state nuclear magnetic resonance [J].Journal of Environmental Quality,31(2):450-456
Menezes-Blackburn D,Giles C,Darch T,et al.2018.Opportunities for mobilizing recalcitrant phosphorus from agricultural soils:a review [J].Plant and Soil,427:5-16
Olaetxeaa M,De Hitaa D,Garcia C A,et al.2018.Hypothetical framework integrating the main mechanisms involved in the promoting action of rhizospheric humic substances on plant root- and shoot-growth [J].Applied Soil Ecology,123:521-537
Parent L-E,IInicki P.2002.Organic Soils and Peat Materials for Sustainable Agriculture [M].Boca Raton:CRC Press
Petrovic M,Kastelan-Macan M.1996.The uptake of inorganic phosphorus by insoluble metal-humic complexes [J].Water Science and Technology,34(7/8):253-258
Roberto B,Urrutia O,Erro J,et al.2016.Humic-(metal)-phosphate complexes characterization and ecological role in phosphorus bioavailability on plant nutrition [C].18th International Conference of International Humic Substances Society.Kanazawa,Japan
Romanyà J,Blanco-Moreno J M,Sans F X.2017.Phosphorus mobilization in low-P arable soils may involve soil organic C depletion [J].Soil Biology and Biochemistry,113:250-259
Senesi N,Plaza C,Brunetti G,et al.2007.A comparative survey of recent results on humic-like fractions in organic amendments and effects on native soil humic substances [J].Soil Biology and Biochemistry,39(6):1244-1262
苏德纯,张福锁,李国学.2000.磷-金属(Fe、Al)-有机酸三元复合体在植物磷营养中的作用[J].土壤通报,31(4):159-161
Stevenson F J.1994.Humus Chemistry:Genesis,Composition,Reactions,second ed [M].New York:Wiley & Sons
Turner B L,Frossard E,Baldwin D S.2005.Organic Phosphorus in the Environment [M].Wallingford:CABI Publishing
Urrutia O,Erro J,Guardado I,et al.2014.Physico-chemical characterization of humic-metal-phosphate complexes and their potential application to the manufacture of new types of phosphate-based fertilizers [J].Journal of Plant Nutrition and Soil Science,177(2):128-136
Wang J,Wu Y,Zhou J,et al.2016.Carbon demand drives microbial mineralization of organic phosphorus during the early stage of soil development [J].Biology and Fertility of Soils,52(6):825-839
王涛,周健民,王火焰.2011.固体废弃物及土壤中磷的形态分析技术[J].土壤学报,48(1):185-191
严玉鹏,万彪,刘凡,等.2012.环境中植酸的分布、形态及界面反应行为[J].应用与环境生物学报,18(3):494-501
袁露露.2018.中国泥炭地资源管理和利用标准体系建立研究[D].长春:东北师范大学
张友,宋佳伟,吕迎春,等.2017.荣成天鹅湖沉积物有机磷的生物有效性及其时空动态[J].环境科学学报,37(12):4629-4635
Zhu Y,Wu F,He Z,et al.2015.Influence of natural organic matter on the bioavailability and preservation of organic phosphorus in lake sediments [J].Chemical Geology,397:51-60
Zhu Y,Wu F,Liu S,et al.2018.Bioavailability and preservation of organic phosphorus in lake sediments:Insights from enzymatic hydrolysis and 31P nuclear magnetic resonance [J].Chemosphere,211:50-61