不同种植年限蕉柑根际土壤线虫群落及营养类群结构特征
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摘要
【目的】分析种植年限对蕉柑根际土壤线虫群落组成与结构特征的影响,探明不同种植年限的蕉柑根际土壤线虫群落多样性与营养类群分布差异的成因。【方法】五点采样法采取土样,浅盘法分离提取线虫,运用生态学指数和主分量分析的PCA排序相结合的方法处理数据。【结果】(1)小树龄蕉柑根际土壤线虫群落的个体密度、属数、多样性指数和丰富度指数均显著高于大树龄蕉柑样地(P<0.05)。(2)PCA排序分析表明,食真菌线虫和大多数食细菌、捕食性/杂食性线虫主要分布在小树龄蕉柑样地,植食性线虫主要分布在大树龄蕉柑样地。(3)相关性分析表明,蕉柑种植年限与土壤环境因子、线虫总密度、线虫属数、生态学指数及食细菌和食真菌线虫、捕食/杂食性线虫密度呈极显著或者显著负相关,与植食性线虫密度呈显著正相关。(4)大树龄蕉柑根际土壤线虫的PPI值和PPI/MI指数显著高于小树龄蕉柑样地(P<0.05)。WI和NCR指数表明,小树龄蕉柑样地土壤健康程度优于种植年限长的大树龄蕉柑样地,小树龄蕉柑样地土壤有机质的分解途径以细菌为主。【结论】果树种植年限的延长会改变土壤的养分与结构,从而降低蕉柑根际土壤线虫密度和群落的多样性,对蕉柑根际土壤线虫各营养类群的结构及分布产生趋向性影响。
【Objective】Planting age plays an important role in the yield and quality of fruits. Environmental factors of soil also are closely related to the yield and quality of fruit, and the growth and development of fruit trees as well. Soil nematode is an indicator organism of soil environment, which can be used to evaluate the quality of soil environment with composition and characteristics of soil nematode community,and the distribution and tendency of nematode nutrient groups. In the present study, nematode communities in rhizospheric soil of Jiaogan(Citrus reticulata Blanco‘Tankan') with different planting years were investigated. In order to ascertain the causes of the distribution differences of the nematode nutrient groups, the effects of planting years on diversity of nematode community were studied by analyzing its composition and structure characteristics.【Methods】Four Jiaogan planting fields with different planting years were chosen as sample fields in Jiaogan planting base of Hongyang town, Jieyang city. Sample fields W1 and S2, with 6 and 7 planting years were set as short time planting fields. Sample fields N1 and 85-2,with 13 and 16 planting years were set as long time planting fields with same field management. In each sample field, three 4 m×4 m lands were set as sample points. Soil samples were taken from the 40 cm depth soil within 1 m range of main roots using 100 cm3 ring samplers. The soils from different layers weremixed evenly before two hundred grams fresh soils were taken from each sample point. Nematodes were isolated from these samples by shallow plate method. Data analysis was performed using the combination of diversity index, evenness index and richness index and PCA ordering of principal component analysis.【Results】(1) The nematodes isolated from the fields with short planting years(W1 and S3) belonged to 29 and 28 genera, with relative abundance of 31.49% and 29.05%, respectively. On the contrary, the nematodes from the fields with long planting years(N1 and 85-2) belonged to 24 and 23 genera, with relative abundance of 18.95% and 20.51%, respectively. The individual densities, genera number, diversity indices and richness indices of the nematodes isolated from the fields with short planting years(W1 and S3)were significantly higher than that from the fields with long planting years(N1 and 85-2)(P<0.05).(2)The results of PCA ordination showed that the first two axes explained the 93.8% change of nematode community, and four axes explained the 97.8% changes of the nematode community. Axis 1 and axis 2 obviously separated the sample field with short(W1 and S3) and long planting years(N1 and 85-2), suggesting that the composition of the soil nematode community in the rhizosphere of Jiaogan distinctly varied with the fields with different planting years. The results also showed difference in nutrient group composition and distribution of the nematodes among the soils from the four sample fields. Fungal nematodes and most eating bacteria, predators/omnivorous nematodes were mainly distributed in the soil with short planting years, whereas planting-feeding nematodes mainly in the soil with long planting years.(3) The correlation analysis indicated that there were significant negative correlations between planting years and the plant biomass, p H, soil organic matter, soil moisture content, soil porosity and soil density and other soil environmental factors, such as total density of nematodes, number of nematode genera, diversity index,richness index and edible bacterial and fungal nematode, predator-prey/omnivorous nematode. A significant positive correlation was found between planting-feeding nematode density and planting years.(4)The PPI value and PPI/MI index of the soil nematodes in the rhizosphere of Jiaogan with long planting years were significantly higher than those with short planting years(P<0.05). The planting-feeding nematodes tended to live in the rhizosphere of Jiaogan with long planting years. The NCR index showed that the decomposition pathway of soil organic matter was mainly functionally related to bacteria in the field with short planting years【.Conclusion】Extension of planting years would result in degeneration of the quality of soil environmental factors, decreasing the density, richness and diversity of soil nematode in the rhizosphere of Jiaogan soil. The change of composition and structure of the nematode nutrient groups in the rhizospheric soil would lead to tendency selection of distribution of eating bacterial nematodes and planting-feeding nematodes.
【Objective】Planting age plays an important role in the yield and quality of fruits. Environmental factors of soil also are closely related to the yield and quality of fruit, and the growth and development of fruit trees as well. Soil nematode is an indicator organism of soil environment, which can be used to evaluate the quality of soil environment with composition and characteristics of soil nematode community,and the distribution and tendency of nematode nutrient groups. In the present study, nematode communities in rhizospheric soil of Jiaogan(Citrus reticulata Blanco‘Tankan') with different planting years were investigated. In order to ascertain the causes of the distribution differences of the nematode nutrient groups, the effects of planting years on diversity of nematode community were studied by analyzing its composition and structure characteristics.【Methods】Four Jiaogan planting fields with different planting years were chosen as sample fields in Jiaogan planting base of Hongyang town, Jieyang city. Sample fields W1 and S2, with 6 and 7 planting years were set as short time planting fields. Sample fields N1 and 85-2,with 13 and 16 planting years were set as long time planting fields with same field management. In each sample field, three 4 m×4 m lands were set as sample points. Soil samples were taken from the 40 cm depth soil within 1 m range of main roots using 100 cm3 ring samplers. The soils from different layers weremixed evenly before two hundred grams fresh soils were taken from each sample point. Nematodes were isolated from these samples by shallow plate method. Data analysis was performed using the combination of diversity index, evenness index and richness index and PCA ordering of principal component analysis.【Results】(1) The nematodes isolated from the fields with short planting years(W1 and S3) belonged to 29 and 28 genera, with relative abundance of 31.49% and 29.05%, respectively. On the contrary, the nematodes from the fields with long planting years(N1 and 85-2) belonged to 24 and 23 genera, with relative abundance of 18.95% and 20.51%, respectively. The individual densities, genera number, diversity indices and richness indices of the nematodes isolated from the fields with short planting years(W1 and S3)were significantly higher than that from the fields with long planting years(N1 and 85-2)(P<0.05).(2)The results of PCA ordination showed that the first two axes explained the 93.8% change of nematode community, and four axes explained the 97.8% changes of the nematode community. Axis 1 and axis 2 obviously separated the sample field with short(W1 and S3) and long planting years(N1 and 85-2), suggesting that the composition of the soil nematode community in the rhizosphere of Jiaogan distinctly varied with the fields with different planting years. The results also showed difference in nutrient group composition and distribution of the nematodes among the soils from the four sample fields. Fungal nematodes and most eating bacteria, predators/omnivorous nematodes were mainly distributed in the soil with short planting years, whereas planting-feeding nematodes mainly in the soil with long planting years.(3) The correlation analysis indicated that there were significant negative correlations between planting years and the plant biomass, p H, soil organic matter, soil moisture content, soil porosity and soil density and other soil environmental factors, such as total density of nematodes, number of nematode genera, diversity index,richness index and edible bacterial and fungal nematode, predator-prey/omnivorous nematode. A significant positive correlation was found between planting-feeding nematode density and planting years.(4)The PPI value and PPI/MI index of the soil nematodes in the rhizosphere of Jiaogan with long planting years were significantly higher than those with short planting years(P<0.05). The planting-feeding nematodes tended to live in the rhizosphere of Jiaogan with long planting years. The NCR index showed that the decomposition pathway of soil organic matter was mainly functionally related to bacteria in the field with short planting years【.Conclusion】Extension of planting years would result in degeneration of the quality of soil environmental factors, decreasing the density, richness and diversity of soil nematode in the rhizosphere of Jiaogan soil. The change of composition and structure of the nematode nutrient groups in the rhizospheric soil would lead to tendency selection of distribution of eating bacterial nematodes and planting-feeding nematodes.
引文
[1]陈金星.不同种植年限苹果园土壤特性比较[D].杨凌:西北农林科技大学,2014.CHEN Jinxing.The comparison of soil charactericsin apple orchards of different cultivation yeas[D].Yangling:Northwest Agriculture and Forestry University,2014.
[2]杜静静,张永清.不同种植年限果园土壤有机质变化特征研究[J].山西师范大学学报(自然科学版),2013,27(2):51-54.DU Jingjing,ZHANG Yongqing.Variations of soil organic matter in apple orchards with different pangting yeas[J].Journal of Shanxi Normal University(Natural Science Edition),2013,27(2):51-54.
[3]张玉树,张金波,朱同彬,蔡祖聪.不同种植年限果园土壤有机氮组分变化特征[J].生态学杂志,2015,34(5):1229-1233.ZHANG Yushu,ZHANG Jinbo,ZHU Tongbin,CAI Zucong.Characteritics of soil organic nitrogen components of orchards with different planting ages[J].Chinese Journal of Ecology,2015,34(5):1229-1233.
[4]王治国,付彦博,饶晓娟,耿庆龙,孟阿静,冯耀祖.不同种植年限果园土壤退化成因分析[J].农业科学与技术:英文版,2014,15(8):1131-1334.WANG Zhiguo,FU Yanbo,RAO Xiaojuan,GENG Qinglong,MENG Ajing,FENG Yaozu.Analysis of soil degradation causes in orchards with different planting years[J].Agricultural Science&Technology,2014,15(8):1331-1334.
[5]王雪山,杜秉海,姚良同,王善林,徐峥,丁延芹.种植年限对牡丹根际土壤微生物群落结构的影响[J].山东大学学报(自然科学版),2012,43(4):508-516.WANG Xueshan,DU Binghai,YAO Liangtong,WANG Shanlin,XU Zheng,DING Yanqin.Effects of planting years on microbial communities structure in peony rhizosphere soil[J].Journal of Shandong Agricutural University(Natural Science),2012,43(4):508-516.
[6]胡秀娜,祁国振,丁延芹,姚良同.种植年限对苹果根际细菌群落结构的影响[C].武汉:全国微生物肥料生产技术研讨会,2014:132-136.HU Xiuna,QI Guozheng,DING Yanqin,YAO Liangtong.Influence of bacterial community structure in apple orchards with planting years[C].Wuhan:National Microbial Fertilizer Production Technology Conference,2014:132-136.
[7]杨树泉,沈向,毛志泉,尹承苗,王峰,王青青.环渤海湾苹果产区老果园与连作果园土壤线虫群落特征[J].生态学报,2010,30(16):4445-4451.YANG Shuquan,SHEN Xiang,MAO Zhiquan,YIN Chengmiao,WANG Feng,WANG Qingqing.Characterization of nematode communities in the soil of long-standing versus replanted apple orchards surrounding Bohai Gulf[J].Acta Ecologica Sinica,2010,30(16):4445-4451.
[8]钟爽,何应对,韩丽娜,周兆禧,马蔚红,曾会才,金志强.连作年限对香蕉园土壤线虫群落结构及多样性的影响[J].中国生态农业学报,2012,20(5):130-139.ZHONG Shuang,HE Yingdui,HAN Lina,ZHOU Zhaoxi,MAWeihong,ZENG Huicai,JIN Zhiqiang.Effect of continuous cropping of banana on soil nematode community structure and diversity[J].Chinese Journal of Eco-Agriculture,2012,20(5):130-139.
[9]刘艳斌,刘奇志,石旺鹏,李红旭,白鹏华,赵明新,刘志龙,周海鹰,王玮,王玉玲.梨园根际土壤线虫对土壤养分及叶片黄化的响应[J].果树学报,2013,30(6):998-1004.LIU Yanbin,LIU Qizhi,SHI Wangpeng,LI Hongxu,BAI Penghua,ZHAO Mingxin,LIU Zhilong,ZHOU Haiying,WANG Wei,WANG Yuling.Response of nematodes to soil nutrient and leaf yellowing in pear trees[J].Journal of Fruit Science,2013,30(6):998-1004.
[10]张航,刘奇志,石旺鹏,刘志龙,周海鹰.北京地区梨园土壤线虫群落组成与多样性研究[J].中国果树,2013,55(2):20-24.ZHANG Hang,LIU Qizhi,SHI Wangpeng,LIU Zhilong,ZHOUHaiying.Studies in soil nematodes community composition and diversity of pear garden in Beijing area[J].China Fruits,2013,55(2):20-24.
[11]孙晓铭,段玉玺,赵磊,陈立杰.辽宁果树根围土壤线虫的多样性研究[J].果树学报,2010,27(3):410-415.SUN Xiaoming,DUAN Yuxi,ZHAO Lei,CHEN Lijie.Diversity of soil nematodes in orchards in Liaoning province[J].Journal of Fruit Science,2010,27(3):410-415.
[12]XIAO H F,TIAN Y H,ZHOU H P,AI X S,YANG X D.Intensive rubber cultivation degrades soil nematode communities in Xishuangbanna,southwest China[J].Soil Biology&Biochemistry,2014,76:161-169.
[13]薛会英,胡锋,罗大庆.藏北高寒草甸植物群落对土壤线虫群落功能结构的影响[J].生态学报,2013,33(5):1482-1494.XUE Huiying,HU Feng,LUO Daqing.Effects of alpine meadow plant communities on soil nematode in Northern Tibet[J].Acta Ecologica Sinica,2014,33(5):1482-1494.
[14]尹文英,胡圣豪,沈韫芬.中国土壤动物检索图鉴[M].北京:科学出版社,1998:51-89.YIN Wenying.HU Shenghao,SHEN Yunfen.Pictorial keys to soil animals of China[M].Beijing:Science Press,1998:51-89.
[15]BONGERS T.De nematode van Nederland[M].Pirota Schoorl Bibliotheek Uitgave Knnv Nr,1988:45-215.
[16]JAIRAJPURI M S,AHMAD W.Dorylaimida:free-living,predaceous and plant-parasitic nematodes[M].Brill,1992:1-458.
[17]谢辉.植物线虫分类学[M].第2版.北京:高等教育出版社,2005:1-384.XIE Hui.Plant nematodes taxonomy[M].2nd edtion.Beijing:Higher Education Press,2005:1-384.
[18]吴纪华.中国淡水和土壤线虫的研究[M].武汉:中国科学院水生物研究所,1999:1-348.WU Jihua.Studies of freshwater and soil nematodes of China[M].Wuhan:Institute of Hydrobiology,Chinese Academy of Science,1999:1-348.
[19]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:146-195.LU Rukun.Methods of soil agricultural chemistry analysis method[M].Beijing:China’s agricultural science and technology press,2000:146-195.
[20]YEATES G W,BONGERS T,DE GOEDE R G M,FRECHMAND W,GEORGIEVA S S.Feeding habits in soil nematode families and genera an outline for soil ecologists[J].Nematol,1993,25(3):315-331.
[21]SHANNON C E,WEAVER W.The mathematical theory of communication[J].University of Illinois press,1949,27(4):623-656.
[22]BONGERS T.The maturity index:An ecological measure of environmental disturbance based on nematode species composition[J].Oecologia,1990,83(1):14-19.
[23]WASILEWSKA L.The effect of age of meadows on succession and diversities in soil nematode communities[J].Pedobiologia,1994,38(1):1-11.
[24]SHANNON C E,WEAVER W.The mathematical theory of communication[M].Chicago:University of Illinois Press:1998:1-144.
[25]戴征凯,赵菊林,宋谨琦,陈永中,刘满强,李辉信,胡锋,焦加国.“绿色南京”林业建设新增林地的土壤生物的群落结构[J].土壤通报,2012,43(1):66-69.DAI Zhengkai,ZHAO Julin,SONG Jinqi,CHEN Yongzhong,LIUManqiang,LI Huixin,HU Feng,JIAO Jiaguo.The community composition of soil microorganisms soil biology in new plangted forest of“Green Nanjing”[J].Chinese Journal of Soil Science,2012,43(1):66-69.
[26]洪毅,张伟东,王雪峰,王诚楠.庄河口湿地不同土地利用方式下土壤线虫群落特征[J].生态环境学报,2014,23(10):1616-1621.HONG Yi,ZHANG Weidong,WANG Xuefeng,WANG Chengnan.The characteristic of soil nematodes communities under different vegetation types in Zhuanghe estuary wetland[J].Ecology and Environmental Sciences,2014,23(10):1616-1621.
[27]STIRLING G R,RAMES E,STIRLING A M,HAMILL S.Factors assciated with the suppressiveness of sugarcane soils to plant-parasitic nematodes[J].Journal of Nematology,2011,43(3/4):135-148.
[28]樊云龙,梁玉华,阮英慧,勾昕.土壤动物在生态监测中的应用[J].贵州科学,2015,33(6):53-57.FAN Yunlong,LIANG Yuhua,RUAN Yinghui,GOU Xin.Application on monitoring of ecology environment with soil animals[J].Guizhou Science,2015,33(6):53-57.
[29]赵艳,阮云泽,张晓波.海南乐东香蕉园土壤线虫群落及多样性研究[J].广东农业科学,2015,42(7):55-59.ZHAO Yan,RUAN Yunze,ZHANG Xiaobo.Soil nematode community structure and diversity in banana garclen in Ledong county of Hainan province[J].Guangdong Agricultural Science,2015,42(7):55-59.
[30]钟爽,曾会才,金志强.不同土壤类型蕉园土壤线虫群落结构的分布特征[J].广东农业科学,2013,40(17):64-67.ZHONG Shuang,ZENG Huicai,JIN Zhiqiang.Distribution characterics of soil nematode community structure in different banana orchards[J].Guangdong Agricultural Science,2013,40(17):64-67.
[31]邢树文,朱慧,庄东红,范红梦,罗国洪,邱少钿.凤凰单丛茶园土壤线虫群落特征[J].生态学杂志,2014,33(3):666-673.XING Shuwen,ZHU Hui,ZHUANG Donghong,FAN Hongmeng,LUO Guohong,QIU Shaotian.Characteristics of soil nematode community in Fenghuang Dancong tea plantations[J].Chinese Journal of Ecology,2014,33(3):666-673.
[32]RITZ K,TRUDGILL D L.Utility of nematode community analysis as an integrated measure of the functional state of soils:Perspectives and challenges[J].Plant Soil,1992,12(1):1-11.
[2]杜静静,张永清.不同种植年限果园土壤有机质变化特征研究[J].山西师范大学学报(自然科学版),2013,27(2):51-54.DU Jingjing,ZHANG Yongqing.Variations of soil organic matter in apple orchards with different pangting yeas[J].Journal of Shanxi Normal University(Natural Science Edition),2013,27(2):51-54.
[3]张玉树,张金波,朱同彬,蔡祖聪.不同种植年限果园土壤有机氮组分变化特征[J].生态学杂志,2015,34(5):1229-1233.ZHANG Yushu,ZHANG Jinbo,ZHU Tongbin,CAI Zucong.Characteritics of soil organic nitrogen components of orchards with different planting ages[J].Chinese Journal of Ecology,2015,34(5):1229-1233.
[4]王治国,付彦博,饶晓娟,耿庆龙,孟阿静,冯耀祖.不同种植年限果园土壤退化成因分析[J].农业科学与技术:英文版,2014,15(8):1131-1334.WANG Zhiguo,FU Yanbo,RAO Xiaojuan,GENG Qinglong,MENG Ajing,FENG Yaozu.Analysis of soil degradation causes in orchards with different planting years[J].Agricultural Science&Technology,2014,15(8):1331-1334.
[5]王雪山,杜秉海,姚良同,王善林,徐峥,丁延芹.种植年限对牡丹根际土壤微生物群落结构的影响[J].山东大学学报(自然科学版),2012,43(4):508-516.WANG Xueshan,DU Binghai,YAO Liangtong,WANG Shanlin,XU Zheng,DING Yanqin.Effects of planting years on microbial communities structure in peony rhizosphere soil[J].Journal of Shandong Agricutural University(Natural Science),2012,43(4):508-516.
[6]胡秀娜,祁国振,丁延芹,姚良同.种植年限对苹果根际细菌群落结构的影响[C].武汉:全国微生物肥料生产技术研讨会,2014:132-136.HU Xiuna,QI Guozheng,DING Yanqin,YAO Liangtong.Influence of bacterial community structure in apple orchards with planting years[C].Wuhan:National Microbial Fertilizer Production Technology Conference,2014:132-136.
[7]杨树泉,沈向,毛志泉,尹承苗,王峰,王青青.环渤海湾苹果产区老果园与连作果园土壤线虫群落特征[J].生态学报,2010,30(16):4445-4451.YANG Shuquan,SHEN Xiang,MAO Zhiquan,YIN Chengmiao,WANG Feng,WANG Qingqing.Characterization of nematode communities in the soil of long-standing versus replanted apple orchards surrounding Bohai Gulf[J].Acta Ecologica Sinica,2010,30(16):4445-4451.
[8]钟爽,何应对,韩丽娜,周兆禧,马蔚红,曾会才,金志强.连作年限对香蕉园土壤线虫群落结构及多样性的影响[J].中国生态农业学报,2012,20(5):130-139.ZHONG Shuang,HE Yingdui,HAN Lina,ZHOU Zhaoxi,MAWeihong,ZENG Huicai,JIN Zhiqiang.Effect of continuous cropping of banana on soil nematode community structure and diversity[J].Chinese Journal of Eco-Agriculture,2012,20(5):130-139.
[9]刘艳斌,刘奇志,石旺鹏,李红旭,白鹏华,赵明新,刘志龙,周海鹰,王玮,王玉玲.梨园根际土壤线虫对土壤养分及叶片黄化的响应[J].果树学报,2013,30(6):998-1004.LIU Yanbin,LIU Qizhi,SHI Wangpeng,LI Hongxu,BAI Penghua,ZHAO Mingxin,LIU Zhilong,ZHOU Haiying,WANG Wei,WANG Yuling.Response of nematodes to soil nutrient and leaf yellowing in pear trees[J].Journal of Fruit Science,2013,30(6):998-1004.
[10]张航,刘奇志,石旺鹏,刘志龙,周海鹰.北京地区梨园土壤线虫群落组成与多样性研究[J].中国果树,2013,55(2):20-24.ZHANG Hang,LIU Qizhi,SHI Wangpeng,LIU Zhilong,ZHOUHaiying.Studies in soil nematodes community composition and diversity of pear garden in Beijing area[J].China Fruits,2013,55(2):20-24.
[11]孙晓铭,段玉玺,赵磊,陈立杰.辽宁果树根围土壤线虫的多样性研究[J].果树学报,2010,27(3):410-415.SUN Xiaoming,DUAN Yuxi,ZHAO Lei,CHEN Lijie.Diversity of soil nematodes in orchards in Liaoning province[J].Journal of Fruit Science,2010,27(3):410-415.
[12]XIAO H F,TIAN Y H,ZHOU H P,AI X S,YANG X D.Intensive rubber cultivation degrades soil nematode communities in Xishuangbanna,southwest China[J].Soil Biology&Biochemistry,2014,76:161-169.
[13]薛会英,胡锋,罗大庆.藏北高寒草甸植物群落对土壤线虫群落功能结构的影响[J].生态学报,2013,33(5):1482-1494.XUE Huiying,HU Feng,LUO Daqing.Effects of alpine meadow plant communities on soil nematode in Northern Tibet[J].Acta Ecologica Sinica,2014,33(5):1482-1494.
[14]尹文英,胡圣豪,沈韫芬.中国土壤动物检索图鉴[M].北京:科学出版社,1998:51-89.YIN Wenying.HU Shenghao,SHEN Yunfen.Pictorial keys to soil animals of China[M].Beijing:Science Press,1998:51-89.
[15]BONGERS T.De nematode van Nederland[M].Pirota Schoorl Bibliotheek Uitgave Knnv Nr,1988:45-215.
[16]JAIRAJPURI M S,AHMAD W.Dorylaimida:free-living,predaceous and plant-parasitic nematodes[M].Brill,1992:1-458.
[17]谢辉.植物线虫分类学[M].第2版.北京:高等教育出版社,2005:1-384.XIE Hui.Plant nematodes taxonomy[M].2nd edtion.Beijing:Higher Education Press,2005:1-384.
[18]吴纪华.中国淡水和土壤线虫的研究[M].武汉:中国科学院水生物研究所,1999:1-348.WU Jihua.Studies of freshwater and soil nematodes of China[M].Wuhan:Institute of Hydrobiology,Chinese Academy of Science,1999:1-348.
[19]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000:146-195.LU Rukun.Methods of soil agricultural chemistry analysis method[M].Beijing:China’s agricultural science and technology press,2000:146-195.
[20]YEATES G W,BONGERS T,DE GOEDE R G M,FRECHMAND W,GEORGIEVA S S.Feeding habits in soil nematode families and genera an outline for soil ecologists[J].Nematol,1993,25(3):315-331.
[21]SHANNON C E,WEAVER W.The mathematical theory of communication[J].University of Illinois press,1949,27(4):623-656.
[22]BONGERS T.The maturity index:An ecological measure of environmental disturbance based on nematode species composition[J].Oecologia,1990,83(1):14-19.
[23]WASILEWSKA L.The effect of age of meadows on succession and diversities in soil nematode communities[J].Pedobiologia,1994,38(1):1-11.
[24]SHANNON C E,WEAVER W.The mathematical theory of communication[M].Chicago:University of Illinois Press:1998:1-144.
[25]戴征凯,赵菊林,宋谨琦,陈永中,刘满强,李辉信,胡锋,焦加国.“绿色南京”林业建设新增林地的土壤生物的群落结构[J].土壤通报,2012,43(1):66-69.DAI Zhengkai,ZHAO Julin,SONG Jinqi,CHEN Yongzhong,LIUManqiang,LI Huixin,HU Feng,JIAO Jiaguo.The community composition of soil microorganisms soil biology in new plangted forest of“Green Nanjing”[J].Chinese Journal of Soil Science,2012,43(1):66-69.
[26]洪毅,张伟东,王雪峰,王诚楠.庄河口湿地不同土地利用方式下土壤线虫群落特征[J].生态环境学报,2014,23(10):1616-1621.HONG Yi,ZHANG Weidong,WANG Xuefeng,WANG Chengnan.The characteristic of soil nematodes communities under different vegetation types in Zhuanghe estuary wetland[J].Ecology and Environmental Sciences,2014,23(10):1616-1621.
[27]STIRLING G R,RAMES E,STIRLING A M,HAMILL S.Factors assciated with the suppressiveness of sugarcane soils to plant-parasitic nematodes[J].Journal of Nematology,2011,43(3/4):135-148.
[28]樊云龙,梁玉华,阮英慧,勾昕.土壤动物在生态监测中的应用[J].贵州科学,2015,33(6):53-57.FAN Yunlong,LIANG Yuhua,RUAN Yinghui,GOU Xin.Application on monitoring of ecology environment with soil animals[J].Guizhou Science,2015,33(6):53-57.
[29]赵艳,阮云泽,张晓波.海南乐东香蕉园土壤线虫群落及多样性研究[J].广东农业科学,2015,42(7):55-59.ZHAO Yan,RUAN Yunze,ZHANG Xiaobo.Soil nematode community structure and diversity in banana garclen in Ledong county of Hainan province[J].Guangdong Agricultural Science,2015,42(7):55-59.
[30]钟爽,曾会才,金志强.不同土壤类型蕉园土壤线虫群落结构的分布特征[J].广东农业科学,2013,40(17):64-67.ZHONG Shuang,ZENG Huicai,JIN Zhiqiang.Distribution characterics of soil nematode community structure in different banana orchards[J].Guangdong Agricultural Science,2013,40(17):64-67.
[31]邢树文,朱慧,庄东红,范红梦,罗国洪,邱少钿.凤凰单丛茶园土壤线虫群落特征[J].生态学杂志,2014,33(3):666-673.XING Shuwen,ZHU Hui,ZHUANG Donghong,FAN Hongmeng,LUO Guohong,QIU Shaotian.Characteristics of soil nematode community in Fenghuang Dancong tea plantations[J].Chinese Journal of Ecology,2014,33(3):666-673.
[32]RITZ K,TRUDGILL D L.Utility of nematode community analysis as an integrated measure of the functional state of soils:Perspectives and challenges[J].Plant Soil,1992,12(1):1-11.