一例鸡传染性法氏囊病病毒的分子鉴定
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摘要
发现一例疑似鸡传染性法氏囊病,利用SPF鸡蛋将传染性法氏囊病料进行病毒增殖,使用PCR技术扩增出IBDV的结构蛋白vp3基因。测序分析结果表明,IBDV vp3长为831 bp,推导其编码277个氨基酸;其与超强毒株日本OKYM和超强毒株香港HK46同源性较高,在核苷酸水平上为98.9%与97.5%,在氨基酸水平上为96.9%和96.2%;对该IBDV进行遗传进化树分析,结果显示,与超强毒株日本OKYM和超强毒株香港HK46位于同一进化分支。因此,从分子水平说明该病毒属于IBDV。
A suspected infectious bursal disease was found, infectious bursal disease virus was proliferated by SPF eggs, and vp3 gene from infectious bursal disease virus was amplified by PCR. The results revealed that the IBDV vp3 gene contained 831 bp nucleotide and 277 amino acid. That compared with very virulent infectious bursal disease virus Japan OKYM strain and Hong Kong HK46 strain had high homology which were 98.9%,97.5% in sequence level and 96.9%,96.2% in amino acid level. The phylogenetic analysis showed that IBDV was in the same lineage with the vv IBDV Japan OKYM strain and Hong Kong HK46 strain. These results demonstrated that this virus was classified to be a IBDV.
A suspected infectious bursal disease was found, infectious bursal disease virus was proliferated by SPF eggs, and vp3 gene from infectious bursal disease virus was amplified by PCR. The results revealed that the IBDV vp3 gene contained 831 bp nucleotide and 277 amino acid. That compared with very virulent infectious bursal disease virus Japan OKYM strain and Hong Kong HK46 strain had high homology which were 98.9%,97.5% in sequence level and 96.9%,96.2% in amino acid level. The phylogenetic analysis showed that IBDV was in the same lineage with the vv IBDV Japan OKYM strain and Hong Kong HK46 strain. These results demonstrated that this virus was classified to be a IBDV.
引文
[1]BECHT H,MLLER H,MLLER H K.Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus[J].Gen.Virol.,1988,63:631-640.
[2]PRINGLE C R.The universal system of virus taxonomy of the International Committee on Virus Taxonomy(ICTV),including new proposals ratified since publication of the Sixth ICTV Report in 1995[J].Archives of Virology,1998,143:203-210.
[3]DAVIS L V S.History of infectious bursal disease in the USA the first two decades[J].Avian Diseases,1997,41(1):11-19.
[4]LOMBARDO E,MARAVER A,CASTN J R,et al.VP1,the putative RNA-dependent RNA polymerase of infectious bursal disease virus,forms complexes with the capsid protein VP3,leading to efficient encapsidation into virus-like paricles[J].Journal of Virology,1999,73(8):6973-6983.
[5]MULLER H,H BECHT.Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their aignificance as structural elements for infectious virus and incomplete particles[J].J.Virol.,1982,44:384-392.
[6]HUDSON P J,MCKERN N M,POWER B E,et al.Genomic structure of the large RNA segment of infectious bursal disease virus[J].Nucleic Acids Research,1986,14(12):5001-5012.
[7]张艳红,吴庆茹,师梦梦,等.IBDV感染对法氏囊RNA m~6A和DNA 5mC修饰以及相关催化酶基因表达的影响[J].中国兽医学报,2019,39(5):936-942.
[8]丁颖楠,俞天奇,张宜娜,等.传染性法氏囊病病毒感染DF-1细胞的lncRNA表达谱变化分析[J].农业生物技术学报,2019(6):1-8.
[9]GARIGLIO MARTA,DABBOU SIHEM,CRISPO MANUELA,et al.Effects of the dietary inclusion of partially defatted black soldier fly(Hermetia illucens)meal on the blood chemistry and tissue(Spleen,Liver,Thymus,and Bursa of Fabricius)histology of muscovy ducks(Cairina moschata domestica)[J].Animals:an Open Access Journal from MDPI,2019,9(6):158-167.
[10]MAHARDIKA G N,BECHT H.Mapping of cross-reacting and serotype-specific epitopes on the VP3 structural protein of the infectious bursal disease virus(IBDV)[J].Archives of Virology,1995,140(4):765-774.
[11]李建亮.传染性法氏囊病病毒山东株的分离鉴定及其鸡胚毒的免疫效果[D].泰安:山东农业大学,2005.
[12]吴卫东,魏中锋,徐梅,等.禽法氏囊、腺病毒二联灭活疫苗的制备与检验[J].中国畜禽种业,2019,15(4):169-170.
[13]陆冰洋,刘华栋,王彩先,等.传染性法氏囊病病毒超强毒株高免卵黄抗体的研制及应用[J].中国家禽,2014(1):22-25.
[14]吴卫东,魏中锋,徐梅,等.鸡法氏囊和腺病毒精制卵黄抗体制备与应用[J].中国畜禽种业,2019,15(5):185-186.
[15]杨棋,魏蔷,刘运超,等.传染性法氏囊病毒VP2蛋白的高效表达与免疫原性分析[J].河南农业科学,2018,47(5):124-128.
[16]冯金芳,何海汛,孙国鹏,等.1株鸡传染性法氏囊病毒VP2基因的克隆及其分子流行病学分析[J].河南农业科学,2018,47(1):109-113,133.
[17]詹丽娥,陆冰洋,刘华栋,等.鸡传染性法氏囊病超强毒株的分子鉴定[J].华北农学报,2013,28(3):234-238.
[18]王占伟,刘茂军,冯志新,等.鸡传染性法氏囊病病毒JSNJ株的分离鉴定及其部分特性研究[J].华北农学报,2010,25(5):47-51.
[2]PRINGLE C R.The universal system of virus taxonomy of the International Committee on Virus Taxonomy(ICTV),including new proposals ratified since publication of the Sixth ICTV Report in 1995[J].Archives of Virology,1998,143:203-210.
[3]DAVIS L V S.History of infectious bursal disease in the USA the first two decades[J].Avian Diseases,1997,41(1):11-19.
[4]LOMBARDO E,MARAVER A,CASTN J R,et al.VP1,the putative RNA-dependent RNA polymerase of infectious bursal disease virus,forms complexes with the capsid protein VP3,leading to efficient encapsidation into virus-like paricles[J].Journal of Virology,1999,73(8):6973-6983.
[5]MULLER H,H BECHT.Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their aignificance as structural elements for infectious virus and incomplete particles[J].J.Virol.,1982,44:384-392.
[6]HUDSON P J,MCKERN N M,POWER B E,et al.Genomic structure of the large RNA segment of infectious bursal disease virus[J].Nucleic Acids Research,1986,14(12):5001-5012.
[7]张艳红,吴庆茹,师梦梦,等.IBDV感染对法氏囊RNA m~6A和DNA 5mC修饰以及相关催化酶基因表达的影响[J].中国兽医学报,2019,39(5):936-942.
[8]丁颖楠,俞天奇,张宜娜,等.传染性法氏囊病病毒感染DF-1细胞的lncRNA表达谱变化分析[J].农业生物技术学报,2019(6):1-8.
[9]GARIGLIO MARTA,DABBOU SIHEM,CRISPO MANUELA,et al.Effects of the dietary inclusion of partially defatted black soldier fly(Hermetia illucens)meal on the blood chemistry and tissue(Spleen,Liver,Thymus,and Bursa of Fabricius)histology of muscovy ducks(Cairina moschata domestica)[J].Animals:an Open Access Journal from MDPI,2019,9(6):158-167.
[10]MAHARDIKA G N,BECHT H.Mapping of cross-reacting and serotype-specific epitopes on the VP3 structural protein of the infectious bursal disease virus(IBDV)[J].Archives of Virology,1995,140(4):765-774.
[11]李建亮.传染性法氏囊病病毒山东株的分离鉴定及其鸡胚毒的免疫效果[D].泰安:山东农业大学,2005.
[12]吴卫东,魏中锋,徐梅,等.禽法氏囊、腺病毒二联灭活疫苗的制备与检验[J].中国畜禽种业,2019,15(4):169-170.
[13]陆冰洋,刘华栋,王彩先,等.传染性法氏囊病病毒超强毒株高免卵黄抗体的研制及应用[J].中国家禽,2014(1):22-25.
[14]吴卫东,魏中锋,徐梅,等.鸡法氏囊和腺病毒精制卵黄抗体制备与应用[J].中国畜禽种业,2019,15(5):185-186.
[15]杨棋,魏蔷,刘运超,等.传染性法氏囊病毒VP2蛋白的高效表达与免疫原性分析[J].河南农业科学,2018,47(5):124-128.
[16]冯金芳,何海汛,孙国鹏,等.1株鸡传染性法氏囊病毒VP2基因的克隆及其分子流行病学分析[J].河南农业科学,2018,47(1):109-113,133.
[17]詹丽娥,陆冰洋,刘华栋,等.鸡传染性法氏囊病超强毒株的分子鉴定[J].华北农学报,2013,28(3):234-238.
[18]王占伟,刘茂军,冯志新,等.鸡传染性法氏囊病病毒JSNJ株的分离鉴定及其部分特性研究[J].华北农学报,2010,25(5):47-51.