不同分子量的褐藻寡糖对黄瓜幼苗光合作用及生长的影响
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摘要
应用不同分子量的褐藻寡糖(ADO)喷施在黄瓜幼苗叶片上,探索不同分子量的褐藻寡糖对黄瓜幼苗的生长情况以及生理特性的影响。结果表明:ADO促进黄瓜幼苗的生长,与对照相比,ADO处理过的幼苗株高、茎粗、株幅和鲜重均有显著增加,且ADO提高了黄瓜叶片中SOD和POD的活力,使得植物体内的氧自由基清除能力提升;ADO还提高叶片中的叶绿素的含量同时使得叶片中净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、胞间CO_2浓度(C_i)、叶绿素荧光参数F_v/F_m值均有显著提高。但是,不同分子量的ADO对黄瓜生长的促进效果存在差异,以分子量为8 000的褐藻寡糖处理的效果最好。
Alginate-derived oligosaccharides of different molecular weight(ADO)were applied to the leave of cucumber seedlings to explore the effects of ADO on the growth and physiological characters of cucumber seedlings.The results showed that:ADO promoted the growth of cucumber seedling in stem diameter,the height and width of seedling;The fresh weight of plant were significantly increased compared with that of control;Moreover,the activities of antioxidant enzymes such as SOD and POD were significantly enhanced in samples treated by ADO.ADO also promoted the synthesis of chlorophyll in cucumber leaves;Meanwhile,photosynthetic rate(P_n),stomatal conductance(G_s),transpiration rate(T_r),intercellular CO_2 concentration(C_i)and chlorophyll fluorescence parameters F_v/F_m were also increased.In conclusion,ADO of different molecular weights of displayed different effects on the growth of cucumber seedling and the treatment of ADO with molecular weight of 8 000 was the best.
Alginate-derived oligosaccharides of different molecular weight(ADO)were applied to the leave of cucumber seedlings to explore the effects of ADO on the growth and physiological characters of cucumber seedlings.The results showed that:ADO promoted the growth of cucumber seedling in stem diameter,the height and width of seedling;The fresh weight of plant were significantly increased compared with that of control;Moreover,the activities of antioxidant enzymes such as SOD and POD were significantly enhanced in samples treated by ADO.ADO also promoted the synthesis of chlorophyll in cucumber leaves;Meanwhile,photosynthetic rate(P_n),stomatal conductance(G_s),transpiration rate(T_r),intercellular CO_2 concentration(C_i)and chlorophyll fluorescence parameters F_v/F_m were also increased.In conclusion,ADO of different molecular weights of displayed different effects on the growth of cucumber seedling and the treatment of ADO with molecular weight of 8 000 was the best.
引文
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[2]马莲菊,卜宁,马纯艳,于翠梅,陈强.褐藻胶寡糖对豌豆种子萌发和幼苗的某些生理特性的影响[J].植物生理学通讯,2007,43(6):1097-110Ma L J,Bu N,Ma C Y,Yu C M,Chen Q.Effects of alginate derived oligosaccharide on seed germination and some physiological characters of seedlings in garden pea(Pisum sativum L)[J].Plant Physiology Communications,2007,43(6):1097-110(in Chinese)
[3]郝林华,陈靠山,李光友.牛蒡寡糖促进黄瓜生长及抗低温胁迫的生理效应[J].上海交通大学学报:农业科学版,2006,24(l):6-12Hao L H,Chen K S,Li G Y.Physiological effects of burdock oligosaccharide on growth promotion and chilling resistance of cucumber seedlings[J].Journal of Shanghai Jiaotong University:Agricultural Science,2006,24(l):6-12(in Chinese)
[4]郭卫华,赵小明,杜昱光.壳寡糖对黄瓜种子萌发和幼苗生长及光合特性的影响[J].中国农学通报,2009,25(3):164-169Guo W H,Zhao X M,Du Y G.Effects ofoligochitosan on the seed germination seedling growth and photosynthetic characters of cucumber[J].Chinese Agricultural Science Bulletin,2009,25(3):164-169(in Chinese)
[5]余劲聪,何舒雅,林克明.海洋寡糖诱导植物抗逆性的研究进展[J].中国农业科技导报,2016,18(4):44-51Yu J C,He S Y,Lin K M.Research progress on plant stress resistance induced by marine oligosaccharides[J].Journal of Agricultural Science and Technology,2016,18(4):44-51(in Chinese)
[6]夏萱.一种海洋寡糖复合植物生长调节剂的研究[D].青岛:中国海洋大学,2012Xia X.Research of a marine oligosaccharide-based complexing plant growth regulator[D].Qingdao:Ocean University of China,2012(in Chinese)
[7]Natsume M,Kamo Y,Hirayama M,Adachi T.Isolation and characterization of alginate derived oligosaccharides with root growth promoting activities[J].Carbohydrate Research,1994,258:187
[8]HienN Q,Nagasawa N,Le X T,Yoshii F,Dang V H,Mitimo H,Makuuchi K,Kume T.Growth-promotion of plants with depolymerized alginate by irradiation[J].Radiation Physics and Chemistry,2000,59:97
[9]刘瑞志.褐藻寡糖促进植物生长与抗逆效应机理研究[D].青岛:中国海洋大学,2009Liu R Z.Effects of alginate derived oligosaccharides on the growth promotion and stress resistance of plants[D].Qingdao:Ocean University of China,2009(in Chinese)
[10]Hu X K,Jiang X L,Hwang H Y,Liu S L,Guan H S.Promotive effects of alginate-derived oligosaccharide on maize seed germination[J].Journal of Applied Phycology,2004,16(1):73-76
[11]Griffiths C A,Sagar R,Geng Y,Primavesi L F,Patel M K,Passarelli M K,Gilmore I S,Steven R T,Bunch J,Paul MJ.Chemical intervention in plant sugar signaling increases yield and resilience[J].Nature,2016,540:574-578
[12]刘瑞志,江晓路,管华诗.褐藻寡糖激发子诱导烟草抗低温作用研究[J].中国海洋大学学报,2009,39(2):243-248Liu R Z,Jiang X L,Guan H S.Effects of alginate-derived oligosaccharide on the low temperature resistance of tobacco leaves[J].Periodical of Ocean University of China,2009,39(2):243-248(in Chinese)
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:258-260,134-137,260-261Li H S.Principles and Techniques of Plant Physiological Biochemical Experiment[M].Beijing:Higher Education Press,2000:258-260,134-137,260-261(in Chinese)
[14]Giannopolitis C N,Ries S K.Superoxide dismutases I.Occurrence in higher plants[J].Plant Physiology,1977,59:309-314
[15]Klapheck S,Zimmer I,Cosse H.Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase[J].Plant and Cell Physiology,1990,31(7):1005-1013
[16]Csiszar J,Galle A,Horvath E,Dancso P,Gombos M,Vary Z,Erdei L,Gyorgyey J,Tari I.Different peroxidase activities and expression of abiotic stress-related peroxidases in apical root segments of wheat genotypes with different drought stress tolerance under osmotic stress[J].Plant Physiology and Biochemistry,2012,52:119-129
[17]Foyer C H,Noctor G.Oxidant and antioxidant signaling in plants:a re-evaluation of the concept of oxidative stress in a physiological context[J].Plant Cell and Environment,2005,28:1056-1071
[18]Nhut D T,Takamura T,Watanabe H,Okamoto K,Tanaka M.Responses of strawberry plantlets cultured in vitro under super bright red and blue light-emitting diodes(LEDs)[J].Plant Cell Tissue and Organ Culture,2003,73:43-52
[19]Sfivastava A,Strasser R J,Govindjee.Differential effects of dimethylbenzoquinone and dichlorobenzoquinone on chlorophyll fluorescence transient in spinach thylakoids[J].Journal of Photochemistry&Photobiology B-Biology,1995,31(3):163-169
[20]Heppich W B,Peckmanm K.Influence of drought on mitochondrial activity,photosynthesis,nocturnal acid accumulation and water relations in the CAM plants Prenia sladeniana(ME-type)and Crassula lycopodioides(PEPCK-type)[J].Annals of Botany,2000,86(3):611-620