乌鲁木齐地区哈萨克族献血人群HPA-15基因多态性分析
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摘要
目的分析乌鲁木齐地区哈萨克族献血人群血小板特异性抗原HPA-15基因多态性,为临床血小板输注提供依据。方法采用聚合酶链-序列特异性引物技术(PCR-SSP)对乌鲁木齐地区100名无血缘关系的哈萨克族献血者的HPA-15系统进行基因分型,计算基因型频率和基因频率。结果哈萨克族献血人群HPA-15a/15a、HPA-15a/15b、HPA-15b/15b基因型频率分别为0.21、0.53、0.26。HPA-15a、HPA-15b的基因频率分别为0.475和0.525。HPA-15a、HPA-15b抗原不配合率分别为0.1997和0.1747。结论乌鲁木齐地区哈萨克族HPA-15抗原不配合概率高,可能导致产生同种免疫性血小板抗体的概率增加,在临床血小板输注中的作用值得重视。
Objective To investigate the genetic polymorphism of HPA-15 alleles in Kazak blood donors from Urumqi area and to provide valuable data for clinical platelet transfusion.Methods HPA-15 system genotype was determined by PCR-SSP method in 100 Kazak blood donors from Urumqi.The frequencies of gene and genotype were calculated and compared with that in other areas.Results The frequencies of HPA-15 aa,HPA-15 ab and HPA-15 bb in Kazak blood donors were 0.21,0.53 and 0.26,respectively.The gene frequency of HPA-15 a and HPA-15 b were 0.475 and 0.525,respectively.The frequencies of antigen incompatibility of HPA-15 a and HPA-15 b were 0.1997 and 0.1747,respectively.Conclusion The frequency of antigen incompatibility of HPA-15 is higher,which indicate HPA-15 may increase the probability of human platelet alloimmunization and may play an important role in clinical platelet transfusion.
Objective To investigate the genetic polymorphism of HPA-15 alleles in Kazak blood donors from Urumqi area and to provide valuable data for clinical platelet transfusion.Methods HPA-15 system genotype was determined by PCR-SSP method in 100 Kazak blood donors from Urumqi.The frequencies of gene and genotype were calculated and compared with that in other areas.Results The frequencies of HPA-15 aa,HPA-15 ab and HPA-15 bb in Kazak blood donors were 0.21,0.53 and 0.26,respectively.The gene frequency of HPA-15 a and HPA-15 b were 0.475 and 0.525,respectively.The frequencies of antigen incompatibility of HPA-15 a and HPA-15 b were 0.1997 and 0.1747,respectively.Conclusion The frequency of antigen incompatibility of HPA-15 is higher,which indicate HPA-15 may increase the probability of human platelet alloimmunization and may play an important role in clinical platelet transfusion.
引文
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9范佳鑫,吐尔洪·克维尔,左宏莉,等.新疆柯尔克孜族人群血小板抗原1~6、15系统基因多态性分析[J].新疆医科大学学报,2011,34(8):820-827.
10夏兰,蔡兴权,陈鑫萍,等.海南岛黎族人血小板1~17抗原系统基因多态性研究[J].中华医学遗传学杂志,2010,27(2):229-230.
11戴宇东.中国汉族人群HPA分型单采血小板供者库的建立模式与库容[J].中国实验血液学杂志,2010,18(4):1046-1050.
12杨兰,梁秀云,曾江辉.广西地区壮族瑶族人群人类血小板抗原基因的多态性分析[J].检验医学与临床,2013,10(20):2644-2645+2647.
13李梦丹,王珏,宋宁,等.拉萨藏族献血人群H P A-1~6、15基因多态性[J].中国输血杂志,2013,26(6):514-517.
14冯明亮,沈伟,黄慧,等.中国汉族人群人类血小板抗原HPA-15(Gov)多态性调查[J].中国输血杂志,2006,19(2):97-99.
2 Metcalfe P,Watkins N A,Ouwehand W H,et al.Nomenclature of human platelet antigens[J].Vox Sanguinis,2003,85(3):240-245.
3 Schuh A C,Watkins N A,Quang N,et al.A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens[J].Blood,2002,99(11):1692-1698
4 Berry J E,Murphy C M,Smith G A,et al.Detection of Gov system antibodies by MAIPA reveals an immunogenicity similar to the HPA-5 alloantigens[J].Br J Haematol,2000,110(3):735-742.
5 Bordin J O,Kelton J O,Warner M N,et al.Maternal immunization to Gov system alloantigens on human platelets[J].Transfusion,1997,37(8):823-828.
6 Feng M L,Liu D Z,Shen W,et al.Establishment of an HPA-1-to-16-typed platelet donor registry in China[J].Transfusion Med,2006,16(5):369-74.
7杨芳,黄吉娥,丛珊,等.贵州黔东南州苗族人群HPA-1~5,1 5多态性调查[J].中国输血杂志,2 0 1 2,2 5(11):1190-1192.
8吐尔洪·克维尔,左宏莉,方春富等.维吾尔族血小板抗原1-5,15系统基因多态性分析[J].新疆医科大学学报,2006,29(12):1128-1130.
9范佳鑫,吐尔洪·克维尔,左宏莉,等.新疆柯尔克孜族人群血小板抗原1~6、15系统基因多态性分析[J].新疆医科大学学报,2011,34(8):820-827.
10夏兰,蔡兴权,陈鑫萍,等.海南岛黎族人血小板1~17抗原系统基因多态性研究[J].中华医学遗传学杂志,2010,27(2):229-230.
11戴宇东.中国汉族人群HPA分型单采血小板供者库的建立模式与库容[J].中国实验血液学杂志,2010,18(4):1046-1050.
12杨兰,梁秀云,曾江辉.广西地区壮族瑶族人群人类血小板抗原基因的多态性分析[J].检验医学与临床,2013,10(20):2644-2645+2647.
13李梦丹,王珏,宋宁,等.拉萨藏族献血人群H P A-1~6、15基因多态性[J].中国输血杂志,2013,26(6):514-517.
14冯明亮,沈伟,黄慧,等.中国汉族人群人类血小板抗原HPA-15(Gov)多态性调查[J].中国输血杂志,2006,19(2):97-99.