碳量子点荧光技术在化学实验教学中的应用
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摘要
将碳量子点荧光技术加到荧光光谱法实验中,使学生在掌握荧光光度计的基本原理、了解荧光分光光度计的构造、学会运用分子荧光光谱法对物质进行定性和定量分析的同时,还可以了解科学前沿的热点,建立节能、绿色环保的观念,做到科研教学相结合,拓展学生的视野,从而提高学生的综合能力。
By adding the carbon quantum dot fluorescence technology into the fluorescence spectroscopy experiment, students can master the basic principle of the fluorescence spectrophotometer, understand the structure of such instrument, learn to use molecular fluorescence spectroscopy to analyze substances qualitatively and quantitatively. At the same time, they can also understand the hot spots in the frontier of science, establish the concept of energy saving and green environmental protection, combine scientific research with teaching, expand their horizons, and improve their comprehensive ability.
By adding the carbon quantum dot fluorescence technology into the fluorescence spectroscopy experiment, students can master the basic principle of the fluorescence spectrophotometer, understand the structure of such instrument, learn to use molecular fluorescence spectroscopy to analyze substances qualitatively and quantitatively. At the same time, they can also understand the hot spots in the frontier of science, establish the concept of energy saving and green environmental protection, combine scientific research with teaching, expand their horizons, and improve their comprehensive ability.
引文
[1] 陈国松,陈昌云.仪器分析实验[M].南京:南京大学出版社,2009.
[2] XU X,RAY R,GU Y,et al.Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments[J].Journal of the American Chemical Society,2004,126(40):12736-12737.
[3] SUN C,ZHANG Y,KALYTCHUK S,et al.Down-conversion monochrome light-emitting diodes with the color determined by the active layer thickness and concentration of carbon dots[J].Journal of Materials Chemistry C,2015,3(26):6613-6615.
[4] CHO S J,MAYSINGER D,JAIN M,et al.Long-term exposure to CdTe quantum dots causes functional impairments in live cells[J].Langmuir,2007,23(4):1974-1980.
[5] LI X,ZHANG S,KULINICH S,et al.Engineering surface states of carbon dots to achieve controllable luminescence for solid-luminescent composites and sensitive Be2+ detection[J].Scientific Reports,2015,4(1):4976.
[6] MIDDLETON E,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacological reviews,2000,52(4):673-751.
[7] SONG Z,QUAN F,XU Y,et al.Multifunctional N,S co-doped carbon quantum dots with pH- and thermo-dependent switchable fluorescent properties and highly selective detection of glutathione[J].Carbon,2016,104:169-178.
[8] LUSARZ A,SHENOUDA N S,SAKLA M S,et al.Common botanical compounds inhibit the hedgehog signaling pathway in prostate cancer[J].Cancer Research,2010,70(8):3382-3390.
[9] CHENG S,GAO N,ZHANG Z,et al.Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax[J].Clinical Cancer Research,2010,16(23):5679-5691.
[10] PSAHOULIA F H,DROSOPOULOS K G,DOUBRAVSKA L,et al.Quercetin enhances TRAIL-mediated apoptosis in colon cancer cells by inducing the accumulation of death receptors in lipid rafts[J].Molecular Cancer Therapeutics,2007,6(9):2591-2599.
[11] 齐德强,王学杰,张一平,等.汞离子荧光分子探针的研究进展[J].浙江外国语学院学报,2012(5):91-101.
[12] WANG F,PANG S,WANG L,et al.One-step synthesis of highly luminescent carbon dots in noncoordinating solvents[J].Chemistry of Materials,2010,22(16):4528-4530.
[13] YANG S T,CAO L,LUO P G,et al.Carbon dots for optical imaging in vivo[J].Journal of the American Chemical Society,2009,131(32):11308-11309.
[14] BOHLE D S,SPINA C J.The relationship of oxygen binding and peroxide sites and the fluorescent properties of zinc oxide semiconductor nanocrystals[J].Journal of the American Chemical Society,2007,129(41):12380-12381.
[2] XU X,RAY R,GU Y,et al.Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments[J].Journal of the American Chemical Society,2004,126(40):12736-12737.
[3] SUN C,ZHANG Y,KALYTCHUK S,et al.Down-conversion monochrome light-emitting diodes with the color determined by the active layer thickness and concentration of carbon dots[J].Journal of Materials Chemistry C,2015,3(26):6613-6615.
[4] CHO S J,MAYSINGER D,JAIN M,et al.Long-term exposure to CdTe quantum dots causes functional impairments in live cells[J].Langmuir,2007,23(4):1974-1980.
[5] LI X,ZHANG S,KULINICH S,et al.Engineering surface states of carbon dots to achieve controllable luminescence for solid-luminescent composites and sensitive Be2+ detection[J].Scientific Reports,2015,4(1):4976.
[6] MIDDLETON E,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacological reviews,2000,52(4):673-751.
[7] SONG Z,QUAN F,XU Y,et al.Multifunctional N,S co-doped carbon quantum dots with pH- and thermo-dependent switchable fluorescent properties and highly selective detection of glutathione[J].Carbon,2016,104:169-178.
[8] LUSARZ A,SHENOUDA N S,SAKLA M S,et al.Common botanical compounds inhibit the hedgehog signaling pathway in prostate cancer[J].Cancer Research,2010,70(8):3382-3390.
[9] CHENG S,GAO N,ZHANG Z,et al.Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax[J].Clinical Cancer Research,2010,16(23):5679-5691.
[10] PSAHOULIA F H,DROSOPOULOS K G,DOUBRAVSKA L,et al.Quercetin enhances TRAIL-mediated apoptosis in colon cancer cells by inducing the accumulation of death receptors in lipid rafts[J].Molecular Cancer Therapeutics,2007,6(9):2591-2599.
[11] 齐德强,王学杰,张一平,等.汞离子荧光分子探针的研究进展[J].浙江外国语学院学报,2012(5):91-101.
[12] WANG F,PANG S,WANG L,et al.One-step synthesis of highly luminescent carbon dots in noncoordinating solvents[J].Chemistry of Materials,2010,22(16):4528-4530.
[13] YANG S T,CAO L,LUO P G,et al.Carbon dots for optical imaging in vivo[J].Journal of the American Chemical Society,2009,131(32):11308-11309.
[14] BOHLE D S,SPINA C J.The relationship of oxygen binding and peroxide sites and the fluorescent properties of zinc oxide semiconductor nanocrystals[J].Journal of the American Chemical Society,2007,129(41):12380-12381.