中山地区住院患儿呼吸道博卡病毒感染的临床研究
|
摘要
目的了解中山地区住院患儿呼吸道博卡病毒(HBoV)感染情况、混合感染及流行病学特点。方法选取2016年7月至2017年7月该院住院的急性呼吸道感染患儿共4 072例作为研究对象,同期完善相关病原学检查,并利用实时荧光定量PC(RT-PCR)对其鼻咽分泌物进行HBoV检测,收集相关资料。结果 4 072例患儿中,HBoV阳性204例,检出率为5.0%,男性患儿126例,阳性构成比61.8%,女性患儿78例,阳性构成比38.2%。<1岁、1～<3岁、3～<7岁,以及≥7岁患儿的HBoV检出率分别为2.04%、1.96%、0.81%、0.15%。从月份上来看,7、8月最高为11.7%,9月为11.3%。从季节上来看,夏、秋季分别为30.9%、25.5%。64例(31.4%)患儿存在混合感染,<1岁26例(12.7%),1～<3岁29例(14.2%),混合感染细菌的最多为42例(20.6%),双重感染24例(11.8%),混合感染的病原体,小于1岁混合感染中呼吸道合胞病毒9例,鼻病毒4例,1～<3岁肺炎支原体感染6例,流感嗜血杆菌6例,鼻病毒3例,3～<7岁及≥7岁主要为MP感染。结论 HBoV是中山地区呼吸道感染的病原体之一,主要感染婴幼儿,混合感染常见,但对病情无明显影响,流行季节在夏秋季,主要临床表现为发热、咳嗽,肝功损害、心肌损害并发症少。
Objective To investigate the infection status,mixed infection and epidemiological characteristics of respiratory human bocavirus in hospitalized children in Zhongshan area.Methods From July 2016 to July2017,a total of 4 072 children with acute respiratory infections were admitted to Boai Hospital in Zhongshan City.At the same time,the related etiology was improved and the HBoV was detected using RT-PCR to collect relevant data.Results A total of 204 children were positive with HBoV,the detection rate was 5.0%,the male were 126,the positive composition was 61.8%,the female children were 78,and the positive composition was 38.2%.The HBoV detection rates for children aged less than 1 year old,1 to<3 years old,3 to<7 years old,and≥7 years old were 2.04%,1.96%,0.81%,and 0.15%respectively.In terms of months,the peak was11.7%in July and August and 11.3% in September.The summer and autumn seasons were 30.9% and25.5%respectively.64% of children(31.4%)had mixed infections,26 cases(12.7%)in the less than 1 year old group,29 cases(14.2%)in the 1-<3 year old group,the highest number of mixed infections is bateria,42 cases(20.6%),and 24 cases of double infections(11.8%).The pathogens of mixed infections were 9 cases of mixed infections of respiratory syncytial virus less than 1 year old,4 cases of rhinovirus,6 cases of MP infection in 1 to<3 years old,6 cases of Haemophilus influenzae,3 cases of rhinovirus,3 to<7 years old and≥7 years old is mainly MP infection.Conclusion HBoV is one of the pathogens of respiratory tract infection in Zhongshan area.It is mainly infected with infants and young children.Mixed infections are common,but there is no significant effect on the condition.In summer and autumn,the main clinical manifestations are fever,cough,liver work damage,and fewer complications of myocardial damage.
Objective To investigate the infection status,mixed infection and epidemiological characteristics of respiratory human bocavirus in hospitalized children in Zhongshan area.Methods From July 2016 to July2017,a total of 4 072 children with acute respiratory infections were admitted to Boai Hospital in Zhongshan City.At the same time,the related etiology was improved and the HBoV was detected using RT-PCR to collect relevant data.Results A total of 204 children were positive with HBoV,the detection rate was 5.0%,the male were 126,the positive composition was 61.8%,the female children were 78,and the positive composition was 38.2%.The HBoV detection rates for children aged less than 1 year old,1 to<3 years old,3 to<7 years old,and≥7 years old were 2.04%,1.96%,0.81%,and 0.15%respectively.In terms of months,the peak was11.7%in July and August and 11.3% in September.The summer and autumn seasons were 30.9% and25.5%respectively.64% of children(31.4%)had mixed infections,26 cases(12.7%)in the less than 1 year old group,29 cases(14.2%)in the 1-<3 year old group,the highest number of mixed infections is bateria,42 cases(20.6%),and 24 cases of double infections(11.8%).The pathogens of mixed infections were 9 cases of mixed infections of respiratory syncytial virus less than 1 year old,4 cases of rhinovirus,6 cases of MP infection in 1 to<3 years old,6 cases of Haemophilus influenzae,3 cases of rhinovirus,3 to<7 years old and≥7 years old is mainly MP infection.Conclusion HBoV is one of the pathogens of respiratory tract infection in Zhongshan area.It is mainly infected with infants and young children.Mixed infections are common,but there is no significant effect on the condition.In summer and autumn,the main clinical manifestations are fever,cough,liver work damage,and fewer complications of myocardial damage.
引文
[1]ALLANDER T,TAMMI M T,ERIKSSON M,et al.Cloning of a human parvovirus by molecular screening of respiratory tract samples[J].Proc Natl Acad Sci U S A,2005,102(36):12891-12896.
[2]GUIDO M,TUMOLO M R,VERRI T,et al.Human bocavirus:current knowledge and future challenges[J].World J Gastroenterol,2016,22(39):8684-8697.
[3]KAPOOR A,SIMMONDS P,SLIKAS E,et al.Human bocaviruses are highly diverse,dispersed,recombination prone,and prevalent in enteric infections[J].J Infect Dis,2010,201(11):1633-1643.
[4]张云宏,兰涛,秦桂秀.人偏肺病毒与人博卡病毒的研究现状[J].中国病毒病杂志,2015,17(5):396-400.
[5]WURZEL D F,MARCHANT J M,CLARK J E,et al.Respiratory virus detection in nasopharyngeal aspirate versus bronchoalveolar lavage is dependent on virus type in children with chronic respiratory symptoms[J].J Clin Virol,2013,58(4):683-688.
[6]XU L,HE X,ZHANG D M,et al.Surveillance and genome analysis of human bocavirus in patients with respiratory infection in Guangzhou,China[J].PLoS One,2012,7(9):e44876.
[7]王瑢,丁淑贤,王娟丽,等.2016年武汉地区住院儿童人博卡病毒感染的临床特征及流行病学[J].中国感染控制杂志,2018,17(1):31-35.
[8]季伟,陈正荣,郭红波,等.苏州儿童医院呼吸道病毒的流行特点及与气候因素的相关性研究[J].中华预防医学杂志,2011,45(3):2205-2207.
[9]JULA A,WARIS M,KANTOLA K,et al.Primary and secondary human bocavirus 1infections in a family,Finland[J].Emerg Infect Dis,2013,19(8):1328-1331.
[10]URSIC T,STEYER A,KOPRIVA S,et al.Human bocavirus as the cause of a life-threatening infection[J].J Clin Microbiol,2011,49(3):1179-1181.
[11]EDNER N,CASTILLO-RODAS P,FALK L,et al.Lifethreatening respiratory tract disease with human bocavirus-1infection in a 4-year-old child[J].J Clin Microbiol,2012,50(2):531-532.
[12]KORNER R W,SODERLUND-VENERMO M,VAN KO-NIN GSBRUGGEN-RIETSCHEL S,et al.Severe human bocavirus infection,Germany[J].Emerg Infect Dis,2011,17(12):2303-2305.
[13]URSIC T,KRIVEC U,KALAN G,et al.Fatal human bocavirus infection in an 18-month-old child with chronic lung disease of prematurity[J].Pediatr Infect Dis J,2015,34(1):111-112.
[14]BREBION A,VANLIEFERINGHEN P,DECHELOTTE P,et al.Fatal subacute myocarditis associated with human bocavirus 2in a 13-month-old child[J].J Clin Microbiol,2014,52(3):1006-1008.
[15]SADEGHI M,KANTOLA K,FINNEGAN D P,et al.Possible involvement of human bocavirus 1in the death of a middle-aged immunosuppressed patient[J].J Clin Microbiol,2013,51(10):3461-3463.
[16]BROCCOLO F,FALCONE V,ESPOSITO S,et al.Human bocaviruses:possible etiologic role in respiratory infection[J].J Clin Virol,2015(72):75-81.
[17]GHIETTO L M,MAJUL D,FERREYRA SOAJE P,et al.Comorbidity and high viral load linked to clinical presentation of respiratory human bocavirus infection[J].Arch Virol,2015,160(1):117-127.
[2]GUIDO M,TUMOLO M R,VERRI T,et al.Human bocavirus:current knowledge and future challenges[J].World J Gastroenterol,2016,22(39):8684-8697.
[3]KAPOOR A,SIMMONDS P,SLIKAS E,et al.Human bocaviruses are highly diverse,dispersed,recombination prone,and prevalent in enteric infections[J].J Infect Dis,2010,201(11):1633-1643.
[4]张云宏,兰涛,秦桂秀.人偏肺病毒与人博卡病毒的研究现状[J].中国病毒病杂志,2015,17(5):396-400.
[5]WURZEL D F,MARCHANT J M,CLARK J E,et al.Respiratory virus detection in nasopharyngeal aspirate versus bronchoalveolar lavage is dependent on virus type in children with chronic respiratory symptoms[J].J Clin Virol,2013,58(4):683-688.
[6]XU L,HE X,ZHANG D M,et al.Surveillance and genome analysis of human bocavirus in patients with respiratory infection in Guangzhou,China[J].PLoS One,2012,7(9):e44876.
[7]王瑢,丁淑贤,王娟丽,等.2016年武汉地区住院儿童人博卡病毒感染的临床特征及流行病学[J].中国感染控制杂志,2018,17(1):31-35.
[8]季伟,陈正荣,郭红波,等.苏州儿童医院呼吸道病毒的流行特点及与气候因素的相关性研究[J].中华预防医学杂志,2011,45(3):2205-2207.
[9]JULA A,WARIS M,KANTOLA K,et al.Primary and secondary human bocavirus 1infections in a family,Finland[J].Emerg Infect Dis,2013,19(8):1328-1331.
[10]URSIC T,STEYER A,KOPRIVA S,et al.Human bocavirus as the cause of a life-threatening infection[J].J Clin Microbiol,2011,49(3):1179-1181.
[11]EDNER N,CASTILLO-RODAS P,FALK L,et al.Lifethreatening respiratory tract disease with human bocavirus-1infection in a 4-year-old child[J].J Clin Microbiol,2012,50(2):531-532.
[12]KORNER R W,SODERLUND-VENERMO M,VAN KO-NIN GSBRUGGEN-RIETSCHEL S,et al.Severe human bocavirus infection,Germany[J].Emerg Infect Dis,2011,17(12):2303-2305.
[13]URSIC T,KRIVEC U,KALAN G,et al.Fatal human bocavirus infection in an 18-month-old child with chronic lung disease of prematurity[J].Pediatr Infect Dis J,2015,34(1):111-112.
[14]BREBION A,VANLIEFERINGHEN P,DECHELOTTE P,et al.Fatal subacute myocarditis associated with human bocavirus 2in a 13-month-old child[J].J Clin Microbiol,2014,52(3):1006-1008.
[15]SADEGHI M,KANTOLA K,FINNEGAN D P,et al.Possible involvement of human bocavirus 1in the death of a middle-aged immunosuppressed patient[J].J Clin Microbiol,2013,51(10):3461-3463.
[16]BROCCOLO F,FALCONE V,ESPOSITO S,et al.Human bocaviruses:possible etiologic role in respiratory infection[J].J Clin Virol,2015(72):75-81.
[17]GHIETTO L M,MAJUL D,FERREYRA SOAJE P,et al.Comorbidity and high viral load linked to clinical presentation of respiratory human bocavirus infection[J].Arch Virol,2015,160(1):117-127.