基于RCWA的蛋白质光学生物传感器的研究
|
摘要
设计了一种蛋白质光学生物传感器,运用严格耦合波(RCWA)方法对该传感器进行理论分析,通过计算机仿真模拟,分析了传感器敏感介质膜系的反射率与激光入射角度和待测蛋白质的折射率、厚度之间的关系。由模拟结果可得:光学生物传感器敏感介质膜系的反射率和激光入射角度与待测蛋白质的折射率、厚度都有良好的线性响应特性,且随着厚度增加灵敏度增加。因此,可以通过检测传感器敏感介质膜系的反射率和入射角度得到待测蛋白质的折射率,通过调控抗体层和抗原层的厚度增减灵敏度。该光学生物传感器可用于检测蛋白质的种类和含量,具有灵敏度高、实时和无标记等优点。
This paper designs protein optical biosensor, theoretically analyzes the sensor by rigorous coupled wave(RCWA) method, analyzes the relationship between the reflectivity of sensitive media membrane system from the sensor and laser incidence angle and the refractive index, thickness of the protein by computer simulation. From the simulation results can be obtained: the reflectivity of sensitive media membrane system from the sensor and the laser incidence angle and refractive index, thickness, incidence angle of the protein has a good linear response characteristic, and the sensitivity increases with the increase of thickness. So that the refractive index of the protein can be obtained by detecting the reflectance of sensitive media membrane system from the sensor and the laser incident angle, and can control the thickness of antibodies and antigens to increase or decrease the sensitivity. The optical biosensors designed can be used to detect the type and content of proteins, it has the advantages of high sensitivity, real-time and no mark.
This paper designs protein optical biosensor, theoretically analyzes the sensor by rigorous coupled wave(RCWA) method, analyzes the relationship between the reflectivity of sensitive media membrane system from the sensor and laser incidence angle and the refractive index, thickness of the protein by computer simulation. From the simulation results can be obtained: the reflectivity of sensitive media membrane system from the sensor and the laser incidence angle and refractive index, thickness, incidence angle of the protein has a good linear response characteristic, and the sensitivity increases with the increase of thickness. So that the refractive index of the protein can be obtained by detecting the reflectance of sensitive media membrane system from the sensor and the laser incident angle, and can control the thickness of antibodies and antigens to increase or decrease the sensitivity. The optical biosensors designed can be used to detect the type and content of proteins, it has the advantages of high sensitivity, real-time and no mark.
引文
[1]BO RISOV S M,WOLFBEIS O S.Optical Biosensor[J].Chemical Reviews,2008,108(2):423-461.
[2]袁小平,何杰,刘荣贵,等.声表面波生物传感器发展与应用[J].压电与声光,2014,36(6):865-871.
[3]HUANG S,LU M,LI B,et al.Real-time detection on the expression of soluble protein and inclusion body in the recombinant escherichia coli with laser tweezers raman spectroscopy[J].Chinese Journal of Lasers,2014(12):251-259.
[4]赵璐,何婷,丁文欢,等.考马斯亮兰法(Bradford法)测定驼乳中蛋白质的含量[J].应用化工,2016,45(12):2366-2368.
[5]王高,冯巧玲,薛忠晋,等.基于共振瑞利散射血清蛋白浓度检测的研究[J].光谱学与光谱分析,2013,33(3):752-755.
[6]WANG Bin,LI Rongzhen,GENG Wenbo,et al.Development and verification of a method for rapid determination of protein content in human coagulation/anticoagulation factor products[J].Chinese Journal of Biologicals,2016,29(4):429-432.
[7]刘海静,许长华,李伟明,等.基于红外指纹图谱的螺旋藻品质分析和蛋白含量测定[J].光谱学与光谱分析,2013,33(4):977-981.
[8]熊贻坤,皇旭光.基于熔融拉锥光纤的液体折射率传感器[J].光学学报,2009,29(7):1956-1960.
[9]曾捷,梁大开,曹振新.光纤SPR传感器测量液体折射率的研究[J].压电与声光,2005,27(1):18-20.
[10]金凤泽,杜春雷,史立芳,等.表面等离子体波成像传感器的实验研究[J].红外与激光工程,2010,39(2):275-278.
[11]NISKANEN I,RTY J,PEIPONEN K E.A method for the detection of the refractive index of irregular shape solid pigments in light absorbing liquid matrix.[J].Talanta,2010,81(4-5):1322-1324.
[12]PU Xiaoya,DU Xusheng,LIU Bo.Determination of Fructose and Glucose Contents in Honey by Liquid Chromatography-refractive Index Detection Method and Direct Titration Method[J].Agricultural Biotechnology,2016(2):31-33.
[13]白忠臣,肖伟,黄政,等.RCWA方法设计阶梯形光学甲醇溶液检测探针[J].压电与声光,2013,35(3):342-344.
[14]LORRAIN N,HIRAOUI M,GUENDOUZ M,et al.Functionalization control of porous silicon optical structures using reflectance spectra modeling for biosensing applications[J].Materials Science&Engineering B,2011,176(14):1047-1053.
[15]ANDERSON G P,GOLDEN J P,LIGLER F S.An evanescent wave biosensor——Part I:Fluorescent signal acquisition from step-etched fiber optic probes.[J].IEEE Transactions on Biomedical Engineering,1994,41(6):578-584.
[16]LIU S,CHEN X.Estimation of the convergence order of rigorous coupled-wave analysis for binary gratings in optical critical dimension metrology[J].Proceedings of SPIE-The International Society for Optical Engineering,2012,51(8):081504-1-081504-7.
[2]袁小平,何杰,刘荣贵,等.声表面波生物传感器发展与应用[J].压电与声光,2014,36(6):865-871.
[3]HUANG S,LU M,LI B,et al.Real-time detection on the expression of soluble protein and inclusion body in the recombinant escherichia coli with laser tweezers raman spectroscopy[J].Chinese Journal of Lasers,2014(12):251-259.
[4]赵璐,何婷,丁文欢,等.考马斯亮兰法(Bradford法)测定驼乳中蛋白质的含量[J].应用化工,2016,45(12):2366-2368.
[5]王高,冯巧玲,薛忠晋,等.基于共振瑞利散射血清蛋白浓度检测的研究[J].光谱学与光谱分析,2013,33(3):752-755.
[6]WANG Bin,LI Rongzhen,GENG Wenbo,et al.Development and verification of a method for rapid determination of protein content in human coagulation/anticoagulation factor products[J].Chinese Journal of Biologicals,2016,29(4):429-432.
[7]刘海静,许长华,李伟明,等.基于红外指纹图谱的螺旋藻品质分析和蛋白含量测定[J].光谱学与光谱分析,2013,33(4):977-981.
[8]熊贻坤,皇旭光.基于熔融拉锥光纤的液体折射率传感器[J].光学学报,2009,29(7):1956-1960.
[9]曾捷,梁大开,曹振新.光纤SPR传感器测量液体折射率的研究[J].压电与声光,2005,27(1):18-20.
[10]金凤泽,杜春雷,史立芳,等.表面等离子体波成像传感器的实验研究[J].红外与激光工程,2010,39(2):275-278.
[11]NISKANEN I,RTY J,PEIPONEN K E.A method for the detection of the refractive index of irregular shape solid pigments in light absorbing liquid matrix.[J].Talanta,2010,81(4-5):1322-1324.
[12]PU Xiaoya,DU Xusheng,LIU Bo.Determination of Fructose and Glucose Contents in Honey by Liquid Chromatography-refractive Index Detection Method and Direct Titration Method[J].Agricultural Biotechnology,2016(2):31-33.
[13]白忠臣,肖伟,黄政,等.RCWA方法设计阶梯形光学甲醇溶液检测探针[J].压电与声光,2013,35(3):342-344.
[14]LORRAIN N,HIRAOUI M,GUENDOUZ M,et al.Functionalization control of porous silicon optical structures using reflectance spectra modeling for biosensing applications[J].Materials Science&Engineering B,2011,176(14):1047-1053.
[15]ANDERSON G P,GOLDEN J P,LIGLER F S.An evanescent wave biosensor——Part I:Fluorescent signal acquisition from step-etched fiber optic probes.[J].IEEE Transactions on Biomedical Engineering,1994,41(6):578-584.
[16]LIU S,CHEN X.Estimation of the convergence order of rigorous coupled-wave analysis for binary gratings in optical critical dimension metrology[J].Proceedings of SPIE-The International Society for Optical Engineering,2012,51(8):081504-1-081504-7.