不同规格野生和养殖刺参体腔细胞数量与体腔液免疫酶活性的比较研究

详细信息    查看全文 | 推荐本文 |

英文篇名：Comparative study on number of coelomocytes and immune enzyme activities in coelomic fluid of wild and cultured sea cucumber with different sizes 作者：梁春蕾 ; 任媛 ; 王轶南 ; 李强 ; 杨欢 ; 李振 英文作者：LIANG Chun-lei;REN Yuan;WANG Yi-nan;LI Qiang;YANG Huan;LI Zhen;College of Fisheries and Life Science, Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University;College of Marine and Biology Engineering, Yancheng Institute of Technology;Dalian SEM Bio-Engineering Technology Company Postdoctoral Research Center; 关键词：刺参 ; 野生 ; 养殖 ; 体腔细胞 ; 非特异免疫酶 英文关键词：Apostichopus japonicus;;wild;;cultured;;coelomocyte;;innate immune enzyme 中文刊名：DLSC 英文刊名：Journal of Dalian Ocean University 机构：大连海洋大学水产与生命学院农业农村部北方海水增养殖重点实验室;盐城工学院海洋与生物工程学院;大连赛姆生物工程技术有限公司博士后工作站; 出版日期：2019-05-14 09:36 出版单位：大连海洋大学学报 年：2019 期：v.34 基金：国家自然科学基金资助项目(31872544);; 盐城工学院人才引进资助项目(XJ201725,XJ201726) 语种：中文; 页：DLSC201903003 页数：7 CN：03 ISSN：21-1575/S 分类号：16-22

摘要

为了比较野生和养殖刺参Apostichopus japonicus及不同规格间的免疫力差异,试验设置3个不同规格的养殖刺参组(体质量分别为30.00 g±9.83 g、70.00 g±7.83 g、110.00 g±10.68 g,记为30 g、70 g、110 g组),以及3个不同规格的野生刺参组(体质量分别为70.00 g±8.13 g、110.00 g±7.53 g、150.00 g±10.64 g,记为70 g、110 g、150 g组),通过比较不同组刺参体腔细胞密度、数量及免疫酶活性等方面的差异,系统地分析了体质量及生活环境与刺参免疫力的关系。结果表明:无论养殖刺参还是野生刺参,不同规格刺参体腔细胞密度无显著性差异(P>0.05),但150 g组野生刺参的球形细胞密度显著高于70 g和110 g组(P<0.05);同种规格下,养殖刺参和野生刺参体腔细胞密度无显著性差异(P>0.05),但110 g体质量组养殖刺参和野生刺参球形细胞和淋巴细胞密度有显著性差异(P<0.05);对于体腔液免疫酶指标,同种类型不同规格刺参差异并不一致,无一定的规律性,但同种规格下,养殖刺参和野生刺参主要免疫酶活力之间无显著性差异(P>0.05)。研究表明,不同生存环境不会影响刺参体腔细胞数量与体腔液免疫活性,本研究结果可为刺参免疫机制的研究提供基础数据。
        In this trial, density, number of coelomocytes and immune enzyme activities were comparatively determined in cultured sea cucumber with body weight of(30.00±9.83)g,(70.00±7.83)g, and(110.00±10.68)g,and wild sea cucumber Apostichopus japonicus with body weight of(70.00±8.13)g,(110.00±7.53)g, and(150.00±10.64)g to explore the immunity relationship between wild and cultured sea cucumber and living environment. The results showed that there was no significant difference in the density of coelomocytes between wild and cultured animals with the different sizes(P>0.05). However, there were significantly higher density of spherulocytes in the wild sea cucumber with body weight of 150 g than that in the wild sea cucumber with body weight of 70 g and 110 g groups(P<0.05). The cultured and wild sea cucumber with the same sizes had no significantly different density of coelomocytes(P>0.05), with significantly higher density of spherulocytes and lymphoid cells in cultured sea cucumber in 110 g group than that in the wild animals(P<0.05). The differences in coelomic fluid parameters were not consistent and no certain regularity was observed in wild and cultured animals, without significant differences in these main immune enzyme activities between cultured and wild sea cucumber with the same size(P>0.05). The findings indicated that the number of coelomocytes and immune enzyme activities of sea cucumber were not influenced by environment, which provided a basis for research of the immune mechanism of sea cucumber.

引文

[1] Li Qiang,Ren Yuan,Liang Chunlei,et al.Regeneration of coelomocytes after evisceration in the sea cucumber,Apostichopus japonicus[J].Fish & Shellfish Immunology,2018,76:266-271.
    [2] Eliseikina M G,Magarlamov T Y.Coelomocyte morphology in the holothurians Apostichopus japonicus (Aspidochirota:Stichopodidae) and Cucumaria japonica(Dendrochirota:Cucumariidae)[J].Russian Journal of Marine Biology,2002,28(3):197-202.
    [3] 刘晓云,谭金山,包振民,等.刺参体腔细胞的超微结构观察[J].电子显微学报,2005,24(6):613-615.
    [4] Xing Kun,Yang Hongsheng,Chen Muyan.Morphological and ultrastructural characterization of the coelomocytes in Apostichopus japonicus[J].Aquatic Biology,2008,2(1):85-92.
    [5] Taguchi M,Tsutsui S,Nakamura O.Differential count and time-course analysis of the cellular composition of coelomocyte aggregate of the Japanese sea cucumber Apostichopus japonicus[J].Fish & Shellfish Immunology,2016,58:203-209.
    [6] 李华,陈静,陆佳,等.仿刺参体腔细胞和血细胞类型及体腔细胞数量研究[J].水生生物学报,2009,33(2):207-213.
    [7] 张宇鹏,田燚,商艳鹏,等.复合免疫增强剂对刺参生长和非特异性免疫酶活性的影响[J].大连海洋大学学报,2017,32(2):178-183.
    [8] Dong Shuanglin,Liang Miao,Gao Qinfeng,et al.Intra-specific effects of sea cucumber (Apostichopus japonicus)with reference to stocking density and body size[J].Aquaculture Research,2010,41(8):1170-1178.
    [9] 朱江艳,张洪彩,陈政强,等.不同规格刺参的非特异性免疫活性研究[J].集美大学学报:自然科学版,2013,18(6):408-412.
    [10] 田丹,姜国良,刘云.不同体质量仿刺参免疫活性的研究[J].海洋科学,2013,37(10):47-52.
    [11] 孟繁伊,麦康森,马洪明,等.棘皮动物免疫学研究进展[J].生物化学与生物物理进展,2009,36(7):803-809.
    [12] 叶海斌,樊英,李天保,等.刺参免疫防御机制研究进展[J].安徽农业科学,2018,46(16):27-29.
    [13] 张琴,麦康森,张文兵,等.饲料中添加硒酵母和维生素E对刺参生长、免疫力及抗病力的影响[J].动物营养学报,2011,23(10):1745-1755.
    [14] 常杰,牛化欣,张文兵,等.刺参免疫系统及其免疫增强剂评价指标的研究进展[J].中国饲料,2011(6):8-12.