基于多波长HPLC指纹图谱结合化学计量分析的金花葵质量评价研究
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摘要
目的建立不同批次金花葵的多波长HPLC指纹图谱,并结合定量分析、相似度评价、聚类分析及主成分分析对金花葵进行质量评价研究。方法采用Phenomenex Kinetex C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.08%磷酸水为流动相,梯度洗脱,体积流量为1.0 mL/min,波长切换(0～8 min,260 nm,检测原儿茶酸;8～15 min,324 nm,检测咖啡酸;15～60min,360nm,检测芦丁、金丝桃苷、异槲皮苷、棉皮素-8-O-β-D-葡萄糖醛酸苷、杨梅素、槲皮素-3′-O-葡萄糖苷、槲皮素),柱温为30℃。利用中药色谱指纹图谱相似度评价系统2004A版及SPSS 19.0软件建立金花葵HPLC指纹图谱,并进行相似度评价、聚类分析及主成分分析。结果金花葵HPLC指纹图谱确认了25个共有峰,指认9个共有峰,并对指认共有峰进行了含量测定。16批金花葵样品相似度在0.879～0.983,相似度良好;聚类分析结果将金花葵聚为2类,表明不同产地金花葵在相似中存在差异性;根据主成分综合得分对金花葵质量进行了排名。结论该方法简便准确,可用于金花葵药材的综合质量评价。
Objective To establish multiwavelength HPLC fingerprint of Aurea helianthus from different batches, and combine quantitative analysis, similarity evaluation, cluster analysis, and principal component analysis to evaluate the quality of A. helianthus. Methods The chromatographic column was Phenomenex Kinetex C18(250 mm × 4.6 mm, 5 μm). The mobile phase was composed of 0.08% phosphoric acid water(A) and acetonitrile(B) in gradient elution at a flow rate of 1.0 mL/min, the detection wavelength was set at 260 nm for protocatechuic acid during 0—8 min, 324 nm for caffeic acid during 8—15 min, 360 nm for rutin, hyperin, isoquercitrin, gossypetin-8-O-β-D-glucuronide, myricetin, quercetin-3′-O-glucoside, and quercetin during 15—60 min. The column temperature was set at 30 oC. And the HPLC fingerprint of A. helianthus was established by the similarity evaluation system for chromatographic fingerprint of TCM(Version 2004 A) and SPSS19.0, which was used for similarity evaluation, cluster analysis, and principal component analysis. Results A total of 25 common peaks were confirmed of A. helianthus HPLC fingerprint, and nine peaks were identified which were determined. The similarity of 16 batches of samples was between 0.879 and 0.983; The results of cluster analysis showed that A. helianthus was clustered into two groups, indicating that there were differences in the similarity; Ranked the quality of A. helianthus based on the main component composite score. Conclusion The method is simple and accurate, which can be used for the comprehensive quality evaluation research of the medicinal materials of A. helianthus.
Objective To establish multiwavelength HPLC fingerprint of Aurea helianthus from different batches, and combine quantitative analysis, similarity evaluation, cluster analysis, and principal component analysis to evaluate the quality of A. helianthus. Methods The chromatographic column was Phenomenex Kinetex C18(250 mm × 4.6 mm, 5 μm). The mobile phase was composed of 0.08% phosphoric acid water(A) and acetonitrile(B) in gradient elution at a flow rate of 1.0 mL/min, the detection wavelength was set at 260 nm for protocatechuic acid during 0—8 min, 324 nm for caffeic acid during 8—15 min, 360 nm for rutin, hyperin, isoquercitrin, gossypetin-8-O-β-D-glucuronide, myricetin, quercetin-3′-O-glucoside, and quercetin during 15—60 min. The column temperature was set at 30 oC. And the HPLC fingerprint of A. helianthus was established by the similarity evaluation system for chromatographic fingerprint of TCM(Version 2004 A) and SPSS19.0, which was used for similarity evaluation, cluster analysis, and principal component analysis. Results A total of 25 common peaks were confirmed of A. helianthus HPLC fingerprint, and nine peaks were identified which were determined. The similarity of 16 batches of samples was between 0.879 and 0.983; The results of cluster analysis showed that A. helianthus was clustered into two groups, indicating that there were differences in the similarity; Ranked the quality of A. helianthus based on the main component composite score. Conclusion The method is simple and accurate, which can be used for the comprehensive quality evaluation research of the medicinal materials of A. helianthus.
引文
[1]张建.金花葵花总黄酮解热抗炎作用的实验研究[J].中国医科大学学报, 2011, 40(8):763-764.
[2]卢丹,贾瑞波.中药金花葵的研究进展[J].中国药物评价, 2015, 32(2):90-93.
[3]曹利华,苗明三.金花葵的现代研究与综合利用分析[J].中医学报, 2016, 31(12):1966-1968.
[4]张春燕,杨倩.金丝桃苷预处理对心肌缺血再灌注性心律失常大鼠心肌ATP酶活性和Cx43、Kir2.1表达的影响[J].中成药, 2018, 40(2):254-260.
[5]宋雨,胡一晨,李维,等.芦丁与槲皮素不同配比对大鼠体内芦丁药动学的影响[J].中国药房, 2016,28(7):902-905.
[6]雷鸣,徐应淑,孙静波,等.异槲皮素对叔丁基过氧化氢诱导的L-02细胞氧化损伤的保护作用[J].天然产物研究与开发, 2017, 29(6):563-568.
[7]吕娜,刘露,李思蒙,等.棉皮素和棉皮苷体外抗氧化、保护DNA及阻断亚硝胺合成作用的比较[J].毒理学杂志, 2016, 30(4):292-296.
[8]林治鑫,顾雪竹,刘珺.高速逆流色谱结合UNIFAC数学模型分离纯化淡竹叶中的槲皮素-3-O-葡萄糖苷[J].中国实验方剂学杂志, 2011, 17(5):23-28.
[9]王博,李德芳,韩吉春,等.杨梅素对离体大鼠心肌缺血再灌注损伤保护作用的研究[J].中国新药杂志,2017, 26(12):1455-1461.
[10]陈家全,王慧,李冬玉,等.原儿茶酸在大鼠体内代谢产物的分析[J].中成药, 2017, 39(3):561-565.
[11]孙皓熠,郝宝燕,张浩超,等.咖啡酸研究概况[J].食品与药品, 2017, 19(2):151-155.
[12]刘东方,赵丽娜,李银峰,等.中药指纹图谱技术的研究进展及应用[J].中草药, 2016, 47(22):4085-4094.
[13]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药, 2013, 44(22):3095-3104.
[14]邵镪钎,李丹,蒋攀,等.基于主成分及聚类分析的川明参的综合评价[J].中草药, 2018, 49(14):3389-3396.
[15]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017, 48(2):278-282.
[2]卢丹,贾瑞波.中药金花葵的研究进展[J].中国药物评价, 2015, 32(2):90-93.
[3]曹利华,苗明三.金花葵的现代研究与综合利用分析[J].中医学报, 2016, 31(12):1966-1968.
[4]张春燕,杨倩.金丝桃苷预处理对心肌缺血再灌注性心律失常大鼠心肌ATP酶活性和Cx43、Kir2.1表达的影响[J].中成药, 2018, 40(2):254-260.
[5]宋雨,胡一晨,李维,等.芦丁与槲皮素不同配比对大鼠体内芦丁药动学的影响[J].中国药房, 2016,28(7):902-905.
[6]雷鸣,徐应淑,孙静波,等.异槲皮素对叔丁基过氧化氢诱导的L-02细胞氧化损伤的保护作用[J].天然产物研究与开发, 2017, 29(6):563-568.
[7]吕娜,刘露,李思蒙,等.棉皮素和棉皮苷体外抗氧化、保护DNA及阻断亚硝胺合成作用的比较[J].毒理学杂志, 2016, 30(4):292-296.
[8]林治鑫,顾雪竹,刘珺.高速逆流色谱结合UNIFAC数学模型分离纯化淡竹叶中的槲皮素-3-O-葡萄糖苷[J].中国实验方剂学杂志, 2011, 17(5):23-28.
[9]王博,李德芳,韩吉春,等.杨梅素对离体大鼠心肌缺血再灌注损伤保护作用的研究[J].中国新药杂志,2017, 26(12):1455-1461.
[10]陈家全,王慧,李冬玉,等.原儿茶酸在大鼠体内代谢产物的分析[J].中成药, 2017, 39(3):561-565.
[11]孙皓熠,郝宝燕,张浩超,等.咖啡酸研究概况[J].食品与药品, 2017, 19(2):151-155.
[12]刘东方,赵丽娜,李银峰,等.中药指纹图谱技术的研究进展及应用[J].中草药, 2016, 47(22):4085-4094.
[13]李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药, 2013, 44(22):3095-3104.
[14]邵镪钎,李丹,蒋攀,等.基于主成分及聚类分析的川明参的综合评价[J].中草药, 2018, 49(14):3389-3396.
[15]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017, 48(2):278-282.