二氧化硅@裸银纳米颗粒微球制备及其对大肠杆菌活性的影响
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摘要
以SiO_2微球为载体,利用其表面可自动吸附与还原银离子的能力,制备新型二氧化硅@银纳米颗粒微球复合材料(SiO_2@Ag NPs),并用透射电镜、Raman光谱、X射线光电子能谱等对其进行表征,测试其对大肠杆菌的抑菌性能.结果表明:当SiO_2@Ag NPs质量浓度为2.0mg/mL时,复合材料的抑菌率为99.8%;当银纳米颗粒表面含有稳定剂聚乙烯吡咯烷酮时,其抑菌率降低.表明稳定剂可保护银纳米颗粒的单分散性,但不利于其抑菌性的发挥.
The silica microspheres were used as carriers to absorb and reduce silver ions automatically on the surface of microspheres,and a new type of composite material of silica@silver nanoparticle microspheres(SiO_2@Ag NPs) was obtained. The silver nanoparticles were characterized by transmission electron microscopy(TEM),Raman spectroscopy and X-ray photoelectron spectroscopy(XPS),and the bacteriostasis of Escherichia coli was tested.The results show that the antibacterial rate of the composite is 99.8% when the mass concentration of SiO_2@Ag NPs is 2.0 mg/mL,and the antibacterial rate decreases when the surface of silver nanoparticles contains stabilizer(polyvinyl pyrrolidone).Therefore,the stabilizer can protect the monodispersity of silver nanoparticles,but it is not conducive to its bacteriostatic effect.
The silica microspheres were used as carriers to absorb and reduce silver ions automatically on the surface of microspheres,and a new type of composite material of silica@silver nanoparticle microspheres(SiO_2@Ag NPs) was obtained. The silver nanoparticles were characterized by transmission electron microscopy(TEM),Raman spectroscopy and X-ray photoelectron spectroscopy(XPS),and the bacteriostasis of Escherichia coli was tested.The results show that the antibacterial rate of the composite is 99.8% when the mass concentration of SiO_2@Ag NPs is 2.0 mg/mL,and the antibacterial rate decreases when the surface of silver nanoparticles contains stabilizer(polyvinyl pyrrolidone).Therefore,the stabilizer can protect the monodispersity of silver nanoparticles,but it is not conducive to its bacteriostatic effect.
引文
[1]何雄,周静峰,孙金才.银纳米粒子基底制备及表面增强拉曼光谱法检测荧光素钠[J].食品安全质量检测学报,2018,9(5):1084-1088.(HE Xiong,ZHOU Jingfeng,SUN Jincai.Preparation of Ag Nanoparticale Substrate and Determination of Sodium Fluorescein by Surface Enhanced Raman Spectroscopy[J].Journal of Food Safety and Quality,2018,9(5):1084-1088.)
[2]徐署东,李卫东.聚多巴胺球支撑银纳米粒子制备无酶过氧化氢生物传感器[J].中国无机分析化学,2016,6(4):79-83.(XU Shudong,LI Weidong.Preparation of Non-enzymatic H2O2Biosensor Based on Polydopamine Spheres Supported Ag Nanoparticle[J].Chinese Journal of Inorganic Analytical Chemistry,2016,6(4):79-83.)
[3]何静妍.银纳米材料的应用研究[J].化工管理,2018(2):152-153.(HE Jingyan.Application of Silver Nanomaterials[J].Chemical Enterprise Management,2018(2):152-153.)
[4]陈顺,刘念,琚艳云,等.四氧化三铁载银纳米粒子的原位制备及其抗菌应用[J].功能材料,2017,48(3):3097-3101.(CHEN Shun,LIU Nian,JU Yanyun,et al.In-situ Synthesis and Antibacterial Application of Fe3O4@Ag Nanoparticle[J].Journal of Functional Materials,2017,48(3):3097-3101.)
[5]陈延明,王晶,李凤红.PVP为稳定剂不同溶剂体系银纳米粒子的制备及表征[J].高分子材料科学与工程,2013,29(2):152-154.(CHEN Yanming,WANG Jing,LI Fenghong.Preparation and Characterization of Silver Nanoparticles Using Polyvinylpyrrolidone as Stabilizer in Different Solvent Systems[J].Polymer Materials Science&Engineering,2013,29(2):152-154.)
[6]周晓光,田春贵,廖丽霞,等.SDS辅助的种子生长法制备片状纳米银[J].黑龙江大学自然科学学报,2012,29(4):507-510.(ZHOU Xiaoguang,TIAN Chungui,LIAO Lixia,et al.SDS-Assisted Seed-Growth Preparation of Plate-Like Ag Nanostructure[J].Journal of Natural Science of Heilongjiang University,2012,29(4):507-510.)
[7]费扬扬.贵金属纳米溶胶的制备及CD光谱研究[D].镇江:江苏科技大学,2016.(FEI Yangyang.The Preparation of Noble Metal Nano-colloidal Solutions and the Study of CD Spectra[D].Zhenjiang:Jiangsu University of Science and Technology,2016.)
[8]张田露.银纳米颗粒致心血管系统毒理学效应及其机制的研究[D].北京:北京印刷学院,2014.(ZHANG Tianlu.Study on the Toxicological Effects and Mechanisms of Silver Nanoparticles on Endothelial System[D].Beijing:Beijing Institute of Graphic Communication,2014.)
[9]刘岩,于清波,白晓,等.溶胶-凝胶法制备单分散功能性二氧化硅球[J].化学研究与应用,2016,28(6):880-883.(LIU Yan,YU Qingbo,BAI Xiao,et al.Preparation of Monodispersed Functional SiO2 Microspheres by a Sol-Gel Method[J].Chemical Research and Application,2016,28(6):880-883.)
[10]李华.平板菌落计数的改进方法[J].生物学通报,2006,41(1):51.(LI Hua.Improvement of Plate Colony Counting Method[J].Bulletin of Biology,2006,41(1):51.)
[11]LIU Yan,LI Yingdi,KANG Yanlei,et al.Silver Nanoparticle Generators:Silicon Dioxide Microspheres[J].Chem Eur J,2017,23(26):6244-6248.
[2]徐署东,李卫东.聚多巴胺球支撑银纳米粒子制备无酶过氧化氢生物传感器[J].中国无机分析化学,2016,6(4):79-83.(XU Shudong,LI Weidong.Preparation of Non-enzymatic H2O2Biosensor Based on Polydopamine Spheres Supported Ag Nanoparticle[J].Chinese Journal of Inorganic Analytical Chemistry,2016,6(4):79-83.)
[3]何静妍.银纳米材料的应用研究[J].化工管理,2018(2):152-153.(HE Jingyan.Application of Silver Nanomaterials[J].Chemical Enterprise Management,2018(2):152-153.)
[4]陈顺,刘念,琚艳云,等.四氧化三铁载银纳米粒子的原位制备及其抗菌应用[J].功能材料,2017,48(3):3097-3101.(CHEN Shun,LIU Nian,JU Yanyun,et al.In-situ Synthesis and Antibacterial Application of Fe3O4@Ag Nanoparticle[J].Journal of Functional Materials,2017,48(3):3097-3101.)
[5]陈延明,王晶,李凤红.PVP为稳定剂不同溶剂体系银纳米粒子的制备及表征[J].高分子材料科学与工程,2013,29(2):152-154.(CHEN Yanming,WANG Jing,LI Fenghong.Preparation and Characterization of Silver Nanoparticles Using Polyvinylpyrrolidone as Stabilizer in Different Solvent Systems[J].Polymer Materials Science&Engineering,2013,29(2):152-154.)
[6]周晓光,田春贵,廖丽霞,等.SDS辅助的种子生长法制备片状纳米银[J].黑龙江大学自然科学学报,2012,29(4):507-510.(ZHOU Xiaoguang,TIAN Chungui,LIAO Lixia,et al.SDS-Assisted Seed-Growth Preparation of Plate-Like Ag Nanostructure[J].Journal of Natural Science of Heilongjiang University,2012,29(4):507-510.)
[7]费扬扬.贵金属纳米溶胶的制备及CD光谱研究[D].镇江:江苏科技大学,2016.(FEI Yangyang.The Preparation of Noble Metal Nano-colloidal Solutions and the Study of CD Spectra[D].Zhenjiang:Jiangsu University of Science and Technology,2016.)
[8]张田露.银纳米颗粒致心血管系统毒理学效应及其机制的研究[D].北京:北京印刷学院,2014.(ZHANG Tianlu.Study on the Toxicological Effects and Mechanisms of Silver Nanoparticles on Endothelial System[D].Beijing:Beijing Institute of Graphic Communication,2014.)
[9]刘岩,于清波,白晓,等.溶胶-凝胶法制备单分散功能性二氧化硅球[J].化学研究与应用,2016,28(6):880-883.(LIU Yan,YU Qingbo,BAI Xiao,et al.Preparation of Monodispersed Functional SiO2 Microspheres by a Sol-Gel Method[J].Chemical Research and Application,2016,28(6):880-883.)
[10]李华.平板菌落计数的改进方法[J].生物学通报,2006,41(1):51.(LI Hua.Improvement of Plate Colony Counting Method[J].Bulletin of Biology,2006,41(1):51.)
[11]LIU Yan,LI Yingdi,KANG Yanlei,et al.Silver Nanoparticle Generators:Silicon Dioxide Microspheres[J].Chem Eur J,2017,23(26):6244-6248.