棉花内生芽孢杆菌LH-L3定殖能力研究
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摘要
为更好地利用优良内生生防菌株,通过自然转化法将携带绿色荧光蛋白基因的质粒p HT01-P43GFPmut3a成功导入生防菌株LH-L3中,获得了标记菌株LH-L3-GFP,用标记菌液浇灌棉株24 h后,在棉株的各个器官中都能检测到标记菌,其定殖量分布由大到小依次为根、茎、花朵和叶。采用涂叶和灌根接种标记菌株的方法,结果发现菌株在蚕豆、玉米、小麦、甘蓝上均可定殖,定殖能力由大到小依次为蚕豆、甘蓝、小麦和玉米。
To make advantage of good beneficial endophyte,a stable GFP tagged strain,LH-L3-GFP wasobtained by transferring plasmid p HT01-P43GFPmut3 a into LH-L3 via natural transformation.The strain wasdistributed in all organs of cotton after its seedling roots drenched with GFP tagged strain in 24 h.The popula-tion was in the order of root>stem>flower>leaf.By inoculation of the GFP tagged strain with painting leaf androots with drenching,the strain could successfully colonize in 4 crops in the order of broad bean > cabbage >wheat>maize.
To make advantage of good beneficial endophyte,a stable GFP tagged strain,LH-L3-GFP wasobtained by transferring plasmid p HT01-P43GFPmut3 a into LH-L3 via natural transformation.The strain wasdistributed in all organs of cotton after its seedling roots drenched with GFP tagged strain in 24 h.The popula-tion was in the order of root>stem>flower>leaf.By inoculation of the GFP tagged strain with painting leaf androots with drenching,the strain could successfully colonize in 4 crops in the order of broad bean > cabbage >wheat>maize.
引文
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[14]蒋晓玲.解淀粉芽孢杆菌Y19微生物菌肥的研制及其生物效益研究[D].昆明:云南农业大学,2015.Jiang X L.Development of microbial fertilizer and biological effect of Bacillus amyloliquefaciens Y19[D].Kunming:YunnanAgricultural University,2015.
[2]彭双,杨茹,闫淑珍,等.杀线虫植物内生细菌和根际放线菌对根结线虫的防效[J].植物保护学报,2012,39(1):63-69.Peng S,Yang R,Yan S Z,et al.Control effect of the antinematode endophytic bacteria and rhizosphere actinomycetes to root-knot nematodes of tomato plant[J].Acta Phytophylacica Sinica,2012,39(1):63-69.
[3]傅本重,朱洁倩,郭新梅,等.核桃内生菌XHE7对病原真菌的拮抗作用及种子发芽的影响[J].江西农业大学学报,2017,39(2):272-277.Fu B Z,Zhu J Q,Guo X M,et al.The antagonism of walnut endophyte XHE7 against plant pathogenic fungi and effect oncrop germination[J].Acta Agriculturae Universitatis Jiangxiensis,2017,39(2):272-277.
[4]杨茹.植物内生细菌对植物寄生线虫、蚜虫和螨虫的活性研究[D].南京:南京师范大学,2013.Yang R.A active products research of the endophytic bacteria for plant-parasite nematode,Myzus persicae and Tetranychuscinnabarinus[D].Nanjing:Nanjing Normal University,2013.
[5]杨洪凤,余向阳,薛雅蓉,等.内生解淀粉芽孢杆菌CC09在小麦根部定殖的电镜观察及防病效果[J].中国生物防治学报,2014,30(6):839-844.Yang H F,Yu X Y,Xue Y R,et al.Colonization of endophytic Bacillus amyloliquefaciens CC09 in wheat roots observed un-der transmission electron microscopy and its biocontrol efficiency against diseases[J].Chinese Journal of Biological Control,2014,30(6):839-844.
[6]杨珍福,何鹏飞,吴毅歆,等.烟草内生细菌YN201448的定殖能力研究[J].中国烟草科学,2015,36(3):80-85.Yang Z F,He P F,Wu Y X,et al.The colonization of endophytic bacterium YN201448 in tobacco[J].Chinese Tobacco Sci-ence,2015,36(3):80-85.
[7]陈丽华,袁德超,吴毅歆,等.棉花黄萎病生防内生芽孢杆菌LH-L3的分离鉴定[J].棉花学报,2017,29(6):550-559.Chen L H,Yuan D C,Wu Y X,et al.Isolation and characterization of endophytic Bacillus methylotrophicus LH-L3 antagonistof cotton Verticillium wilt[J].Cotton Science,2017,29(6):550-559.
[8]何鹏飞.B9601-Y2菌株的基因组解析及部分功能验证[D].武汉:华中农业大学,2014.He P F.Genomic analysis of B9601-Y2 strain and partial functions confirmation[D].Wuhan:Huazhong Agricultural Univer-sity,2014.
[9]方中达.植病研究方法[M].北京:中国农业出版社,1998.Fang Z D.Research methods of plant pathology[M].Beijing:China agricultural press,1998.
[10]Avis T J,Gravel V,Antoun H,et al.Multifaceted beneficial effects of rhizosphere microorganisms on plant health and pro-ductivity[J].Soil Biology and Biochemistry,2008,40(7):1733-1740.
[11]Klein E,Ofek M,Katan J,et al.Soil suppressiveness to Fusarium disease:shifts in root microbiome associated with reductionof pathogen root colonization[J].Phytopathology,2013,103(1):23-33.
[12]王娜,陶伟,陈双林,等.植物内生细菌在棉花体内的定殖动态及对棉花黄萎病的生物防治效果[J].植物保护学报,2016,43(2):207-214.Wang N,Tao W,Chen S L,et al.Colonization trends of endophytic bacteria in cotton and their biological control effect oncotton verticillium wilt[J].Journal of Plant Protection,2016,43(2):207-214.
[13]陈楠,于飞,何艳柳.一株水稻促生长内生真菌的绿色荧光蛋白基因标记与示踪[J].生物技术通报,2017,33(3):100-105.Chen N,Yu F,He Y L.Labeling and tracing of green fluorescent protein in fungal endophyte with growth-promoting activityto rice seedlings[J].Biotechnology Bulletin,2017,33(3):100-105.
[14]蒋晓玲.解淀粉芽孢杆菌Y19微生物菌肥的研制及其生物效益研究[D].昆明:云南农业大学,2015.Jiang X L.Development of microbial fertilizer and biological effect of Bacillus amyloliquefaciens Y19[D].Kunming:YunnanAgricultural University,2015.