穴位贴敷对过敏性鼻炎大鼠炎性反应的调节作用
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摘要
目的:观察穴位贴敷对过敏性鼻炎(AR)大鼠血清免疫球蛋白E(IgE)、转化生长因子beta 1(TGF-β1)和鼻黏膜嗜酸性粒细胞(EOS)的影响,探讨穴位贴敷对AR的作用机制。方法:Wistar大鼠随机分为正常对照组、模型组、药物组和穴贴组,每组10只。采用卵白蛋白注射及鼻黏膜刺激法建立AR大鼠模型。穴贴组大鼠予双侧"肺俞""脾俞""肾俞"穴位贴敷,隔日1次,药物组每日予丙酸氟替卡松鼻喷雾剂滴鼻,均治疗14d。观察大鼠治疗前后行为学评分,酶联免疫吸附法检测大鼠血清中IgE、TGF-β1的含量,苏木素伊红染色观察大鼠鼻黏膜EOS浸润情况。结果:与正常对照组比较,模型组大鼠行为学评分及血清IgE、TGF-β1水平明显上升(P<0.05),鼻黏膜EOS数量明显增加;与模型组比较,药物组和穴贴组大鼠行为学评分及血清IgE、TGF-β1水平均明显降低(P<0.05),鼻黏膜无明显EOS浸润;穴贴组与药物组比较,各指标差异无统计学意义(P>0.05)。结论:穴位贴敷对AR的治疗效果显著,其对AR的调节作用可能是通过下调血清中IgE、TGF-β1水平而实现的。
Objective To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis(AR),serum immunoglobulin E(IgE)and transforming growth factor beta 1(TGF-β1)level,and number of nasal eosinophils(EOS)in rats with AR,so as to explore its underlying mechanisms.Methods Forty male Wistar rats were randomly divided into normal control,model,medication and acupoint application groups(n=10 rats per group).The AR model was established by intraperitoneal(i.p.)injection of mixture solution of ovalbumin,aluminum hydroxide and normal saline(once every other day,for7 times),and nasal drip plus spray inhalation of ovalbumin(on the following day of i.p.,once daily for 9 days).For acupoint application,the prepared herbal paste(containing White Mustard Seed,Rhizoma Corydalis,unprocessed Radix Kansui,Herba Asari and ginger juice)was applied to bilateral"Feishu"(BL13),"Pishu"(BL20)and"Shenshu"(BL23)for 2 h,once every other day for 7 times.The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days.Scores of nasal itching,sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum IgE and TGF-β1,and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.Results After modeling and compared with the normal control group,the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher(P<0.05),and the infiltration state of EOS got worse.Compared with the model group,the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed(P<0.05)and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups.No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels(P>0.05).Conclusion Acupoint application can improve the symptoms of AR rats,which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1.
Objective To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis(AR),serum immunoglobulin E(IgE)and transforming growth factor beta 1(TGF-β1)level,and number of nasal eosinophils(EOS)in rats with AR,so as to explore its underlying mechanisms.Methods Forty male Wistar rats were randomly divided into normal control,model,medication and acupoint application groups(n=10 rats per group).The AR model was established by intraperitoneal(i.p.)injection of mixture solution of ovalbumin,aluminum hydroxide and normal saline(once every other day,for7 times),and nasal drip plus spray inhalation of ovalbumin(on the following day of i.p.,once daily for 9 days).For acupoint application,the prepared herbal paste(containing White Mustard Seed,Rhizoma Corydalis,unprocessed Radix Kansui,Herba Asari and ginger juice)was applied to bilateral"Feishu"(BL13),"Pishu"(BL20)and"Shenshu"(BL23)for 2 h,once every other day for 7 times.The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days.Scores of nasal itching,sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum IgE and TGF-β1,and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.Results After modeling and compared with the normal control group,the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher(P<0.05),and the infiltration state of EOS got worse.Compared with the model group,the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed(P<0.05)and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups.No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels(P>0.05).Conclusion Acupoint application can improve the symptoms of AR rats,which may be associated with its effect in down-regulating the levels of serum IgE and TGF-β1.
引文
[1]陈静,陈莹,张继苹,等.中药超微粉腧穴敷贴对变应性鼻炎大鼠IFN-γ和IL-4含量的影响[J].时珍国医国药,2014,25(11):2813-2815.
[2]谢冰斌,刘月辉,朱新华,等.丙酸氟替卡松对转化生长因子β1蛋白及其mRNA在大鼠变应性鼻炎鼻黏膜中表达的影响[J].中国耳鼻咽喉头颈外科,2011,18(7):381-384.
[3]朱歆洁,朱鲁平,陆美萍,等.儿童变应性鼻炎严重度与TG-FB1基因-509C/T多态性的关系[J].中华耳鼻咽喉头颈外科杂志,2010,45(6):459-464.
[4]秦乐,曾强,欧云娜,等.过敏性鼻炎的治疗进展[J].中药与临床,2014,5(4):50-55.
[5]SEIDMAN M D,GURGEL R K,LIN S Y,et al.Clinical practice guideline:allergic rhinitis[J].Otolaryngol Head Neck Surg,2015,152(1Suppl):S1-S43.
[6]WEN C Y,LIU Y F,ZHOU L,et al.A systematic and narrative review of acupuncture point application therapies in the treatment of allergic rhinitis and asthma during dog days[J].Evid Based Complement Alternat Med,2015,2015:846851.
[7]李忠仁.实验针灸学[M].2版.北京:中国中医药出版社,2003.
[8]谢冰斌,刘月辉,刘建国,等.丙酸氟替卡松对变应性鼻炎鼻黏膜重塑动态影响的实验研究[J].临床耳鼻咽喉头颈外科杂志,2011,25(5):232-235.
[9]刘永平,陈蕴光,郑俊斌,等.变应性鼻炎鼻激发后鼻腔分泌物嗜酸性粒细胞增加与血清特异性IgE水平正相关[J].细胞与分子免疫学杂志,2017,33(1):85-88.
[10]张元,侯珣瑞,李丽红,等.穴位注射对变应性鼻炎大鼠鼻黏膜中嗜酸性粒细胞计数、嗜酸性粒细胞趋化因子蛋白和mRNA表达的影响[J].针刺研究,2017,42(2):141-144.
[11]OUYANG Y,MIYATA M,HATSUSHIKA K,et al.TGF-beta signaling may play a role in the development of goblet cell hyperplasia in a mouse model of allergic rhinitis[J].Allergol Int,2010,59(3):313-319.
[12]朱歆洁,朱鲁平,程雷,等.转化生长因子β在过敏性鼻炎发病机制中的作用[J].中华临床免疫和变态反应杂志,2010,4(4):294-299.
[13]李科婷,刘涛.免疫治疗调节变应性鼻炎外周血调节性T细胞表达[J].国际耳鼻咽喉头颈外科杂志,2017,41(3):175-177.
[14]孟珣,沙骥超,董震,等.调节性T细胞在变应性鼻炎中的研究进展[J].中国实验诊断学,2013,17(3):585-587.
[15]陈德华,李添应.干扰素-γ诱导的单核因子、单核细胞趋化蛋白-1和转化生长因子-β1在变应性鼻炎的表达及其意义[J].中华临床免疫和变态反应杂志,2010,4(2):103-106.
[2]谢冰斌,刘月辉,朱新华,等.丙酸氟替卡松对转化生长因子β1蛋白及其mRNA在大鼠变应性鼻炎鼻黏膜中表达的影响[J].中国耳鼻咽喉头颈外科,2011,18(7):381-384.
[3]朱歆洁,朱鲁平,陆美萍,等.儿童变应性鼻炎严重度与TG-FB1基因-509C/T多态性的关系[J].中华耳鼻咽喉头颈外科杂志,2010,45(6):459-464.
[4]秦乐,曾强,欧云娜,等.过敏性鼻炎的治疗进展[J].中药与临床,2014,5(4):50-55.
[5]SEIDMAN M D,GURGEL R K,LIN S Y,et al.Clinical practice guideline:allergic rhinitis[J].Otolaryngol Head Neck Surg,2015,152(1Suppl):S1-S43.
[6]WEN C Y,LIU Y F,ZHOU L,et al.A systematic and narrative review of acupuncture point application therapies in the treatment of allergic rhinitis and asthma during dog days[J].Evid Based Complement Alternat Med,2015,2015:846851.
[7]李忠仁.实验针灸学[M].2版.北京:中国中医药出版社,2003.
[8]谢冰斌,刘月辉,刘建国,等.丙酸氟替卡松对变应性鼻炎鼻黏膜重塑动态影响的实验研究[J].临床耳鼻咽喉头颈外科杂志,2011,25(5):232-235.
[9]刘永平,陈蕴光,郑俊斌,等.变应性鼻炎鼻激发后鼻腔分泌物嗜酸性粒细胞增加与血清特异性IgE水平正相关[J].细胞与分子免疫学杂志,2017,33(1):85-88.
[10]张元,侯珣瑞,李丽红,等.穴位注射对变应性鼻炎大鼠鼻黏膜中嗜酸性粒细胞计数、嗜酸性粒细胞趋化因子蛋白和mRNA表达的影响[J].针刺研究,2017,42(2):141-144.
[11]OUYANG Y,MIYATA M,HATSUSHIKA K,et al.TGF-beta signaling may play a role in the development of goblet cell hyperplasia in a mouse model of allergic rhinitis[J].Allergol Int,2010,59(3):313-319.
[12]朱歆洁,朱鲁平,程雷,等.转化生长因子β在过敏性鼻炎发病机制中的作用[J].中华临床免疫和变态反应杂志,2010,4(4):294-299.
[13]李科婷,刘涛.免疫治疗调节变应性鼻炎外周血调节性T细胞表达[J].国际耳鼻咽喉头颈外科杂志,2017,41(3):175-177.
[14]孟珣,沙骥超,董震,等.调节性T细胞在变应性鼻炎中的研究进展[J].中国实验诊断学,2013,17(3):585-587.
[15]陈德华,李添应.干扰素-γ诱导的单核因子、单核细胞趋化蛋白-1和转化生长因子-β1在变应性鼻炎的表达及其意义[J].中华临床免疫和变态反应杂志,2010,4(2):103-106.