河北省番茄黄化曲叶病毒和番茄褪绿病毒复合侵染及分布
|
摘要
番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)及番茄褪绿病毒(Tomato chlorosis virus,ToCV)是危害番茄的主要病毒。2016～2017年,在河北省18个番茄主产区调查番茄病毒病危害情况,采集262份植株矮化、叶片黄化、褪绿、卷曲的番茄样品,利用分子生物学技术对病原进行鉴定。结果表明:2016～2017年河北省番茄病毒病发生普遍,所检样品中TYLCV的侵染率为68.66%,其中与ToCV复合侵染率为19.5%;除栾城、滦南、乐亭、永年4地样品暂未检测到ToCV外,其他14个采样点的样品均检测到ToCV,侵染率为19.5%,且全部表现为与TYLCV复合侵染,河北省番茄主产区暂未发现ToCV单独侵染的样品。
Tomato yellow leaf curl virus and Tomato chlorosis virus were two major virus diseases in China.During 2016-2017,262 leaf samples,which showed symptoms of stunting,yellowing,curling,chlorosis and rolling were collected from 18 main tomato-producing regions. Molecular and biological methods detection were used to detect TYLCV and ToCV. The results showed that virus diseases occurred in all the tomato-cultivated areas throughout the Hebei province. The symptoms of tomato virus disease in filed were complex. The infection rates of TYLCV,detected in every sampling site,were 68.66%,where the mixed infection rates with ToCV were 19.5%,and the rest were singly infected. ToCV was detected in all regions except for Luan cheng,Luan nan,Le ting and Yong nian. The infection rate of ToCV was 19.5%,and all the positive samples were mixinfected with TYLCV. No single infection of ToCV on tomato was found.
Tomato yellow leaf curl virus and Tomato chlorosis virus were two major virus diseases in China.During 2016-2017,262 leaf samples,which showed symptoms of stunting,yellowing,curling,chlorosis and rolling were collected from 18 main tomato-producing regions. Molecular and biological methods detection were used to detect TYLCV and ToCV. The results showed that virus diseases occurred in all the tomato-cultivated areas throughout the Hebei province. The symptoms of tomato virus disease in filed were complex. The infection rates of TYLCV,detected in every sampling site,were 68.66%,where the mixed infection rates with ToCV were 19.5%,and the rest were singly infected. ToCV was detected in all regions except for Luan cheng,Luan nan,Le ting and Yong nian. The infection rate of ToCV was 19.5%,and all the positive samples were mixinfected with TYLCV. No single infection of ToCV on tomato was found.
引文
[1]Fauquet C M,Mayo M A,Maniloff J,et al.Virus taxonomy:The Eighth Report of the International Committee on Taxonomy of Viruses[R].San Diego:Elsevier Academic Press,2005.
[2]Wu J B,Dai F M,Zhou X P.First report of Tomato yellow leaf curl virus in China[J].Plant Disease,2006,90(10):1359.
[3]Tian P,Ai P F,Song J J.et al.The progress of sequence analysis,spread and distribution of w hiteflytransmitted geminivirus in tomato in China(in Chinese)[J].Chinese Agricultural Science Bulletin(中国农学通报),2013,29(1):60-64.
[4]Di D P,Gong H R,Zhang S M,et al.Tomato yellow leaf curl virus disease-new tomato disease in Hebei Province(in Chinese)[A].Annual Conference of China Society of Plant Protection[C].(Beijing:China Agricultural Science and Technology Press),2009:207-209.
[5]Zhang A H,Zhang S M,Liu S,et al.Occurrence and distribution of Tomato yellow leaf curl disease in Hebei Province(in Chinese)[J].Plant Protection(植物保护),2010,36(4):127-129.
[6]Zhou Y,Li X H,Liu J H,et al.Molecular identification of Tomato yellow leaf curl virus in Hebei(in Chinese)[J].Plant Protection(植物保护),2010,36(1):60-64.
[7]King A M Q,Lefkowitz E,Adams M J,et al.Virus taxonomy:The ninth report of the International committee on taxonomy of viruses[R].Amsterdam:Elsevier Academic Press,2011.
[8]Sun G Z,Gao L L,Lu W L,et al.Molecular detection and identification of Tomato chlorosis virus infecting greenhouse grow n tomato plants in Hebei Province(in Chinese)[J].Northern Horticulture(北方园艺),2015,(9):95-98.
[9]Zhou T,Yang P Y,Zhao R N,et al.Alarming the transmission and damage of Tomato chlorosis virus in China(in Chinese)[J].Plant Protection(植物保护),2014,40(5):196-199.
[10]Zhang A H,Zhao C N,Di D P,et al.Identification of the resistance of tomato varieties to TYLCV in Hebei Province(in Chinese)[J].Plant Protection(植物保护),2011,37(5):148-150.
[11]Du K T,Wang Z J,Yong R Q,et al.Molecular identification and full-length sequence analysis of Tomato yellow leaf curl virus on sw eet pepper in China(in Chinese)[J].Acta Phytopathologica Sinica(植物病理学报),2016,46(3):425-428.
[12]Liu Y G,Wei J P,Qiao N,et al.Tomato chlorosis virus outbreaks in Shandong and prevention measures(in Chinese)[J].China Vegetables(中国蔬菜),2014,(5):67-69.
[13]Zhao R N,Wang R,Shi Y C,et al.Molecular identification of Tomato chlorosis virus on sw eet pepper(in Chinese)[J].Plant Protection(植物保护),2014,40(1):128-130.
[14]Zhao L M.The establishment of the molecular identification,complete genome sequence analysis,and detection technique of Tomato chlorosis virus(in Chinese)[D].Tai'an:Shandong Agricultural University(泰安:山东农业大学),2015.
[15]Wang C L,Feng J,Sun X H,et al.Molecular identification of Tomato chlorosis virus from four provinces or autonomous region in northern China(in Chinese)[J].Plant Protection(植物保护),2017,43(2):141-145.
[16]Hu J A,Wan X J,Li Z J,et al.Molecular identification of Tomato chlorosis virus in Henan Province(in Chinese)[J].China Vegetables(中国蔬菜),2015(12):25-28.
[17]Fondong V N,Pita J S,Rey M E C,et al.Evidence of synergism betw een African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon[J].Journal of General Virology,2000,81:287-297.
[18]Tatineni S,Graybosch R A,Hein G L,et al.Wheat cultivar-specific disease synergism and alteration of virus accumulation during co-infection w ith Wheat streak mosaic virus and Triticum mosaic virus[J].Phytopathology,2010,100(3):230-238.
[19]Srinivasan R,Alvarez J M.Effect of mixed viral infections(Potato virus Y-Potato leafroll virus)on biology and preference of vectors Myzus persicae and Macrosiphum euphorbiae(Hemiptera;Aphididae)[J].Journal of Economic Entomology,2007,100(3):646-655.
[2]Wu J B,Dai F M,Zhou X P.First report of Tomato yellow leaf curl virus in China[J].Plant Disease,2006,90(10):1359.
[3]Tian P,Ai P F,Song J J.et al.The progress of sequence analysis,spread and distribution of w hiteflytransmitted geminivirus in tomato in China(in Chinese)[J].Chinese Agricultural Science Bulletin(中国农学通报),2013,29(1):60-64.
[4]Di D P,Gong H R,Zhang S M,et al.Tomato yellow leaf curl virus disease-new tomato disease in Hebei Province(in Chinese)[A].Annual Conference of China Society of Plant Protection[C].(Beijing:China Agricultural Science and Technology Press),2009:207-209.
[5]Zhang A H,Zhang S M,Liu S,et al.Occurrence and distribution of Tomato yellow leaf curl disease in Hebei Province(in Chinese)[J].Plant Protection(植物保护),2010,36(4):127-129.
[6]Zhou Y,Li X H,Liu J H,et al.Molecular identification of Tomato yellow leaf curl virus in Hebei(in Chinese)[J].Plant Protection(植物保护),2010,36(1):60-64.
[7]King A M Q,Lefkowitz E,Adams M J,et al.Virus taxonomy:The ninth report of the International committee on taxonomy of viruses[R].Amsterdam:Elsevier Academic Press,2011.
[8]Sun G Z,Gao L L,Lu W L,et al.Molecular detection and identification of Tomato chlorosis virus infecting greenhouse grow n tomato plants in Hebei Province(in Chinese)[J].Northern Horticulture(北方园艺),2015,(9):95-98.
[9]Zhou T,Yang P Y,Zhao R N,et al.Alarming the transmission and damage of Tomato chlorosis virus in China(in Chinese)[J].Plant Protection(植物保护),2014,40(5):196-199.
[10]Zhang A H,Zhao C N,Di D P,et al.Identification of the resistance of tomato varieties to TYLCV in Hebei Province(in Chinese)[J].Plant Protection(植物保护),2011,37(5):148-150.
[11]Du K T,Wang Z J,Yong R Q,et al.Molecular identification and full-length sequence analysis of Tomato yellow leaf curl virus on sw eet pepper in China(in Chinese)[J].Acta Phytopathologica Sinica(植物病理学报),2016,46(3):425-428.
[12]Liu Y G,Wei J P,Qiao N,et al.Tomato chlorosis virus outbreaks in Shandong and prevention measures(in Chinese)[J].China Vegetables(中国蔬菜),2014,(5):67-69.
[13]Zhao R N,Wang R,Shi Y C,et al.Molecular identification of Tomato chlorosis virus on sw eet pepper(in Chinese)[J].Plant Protection(植物保护),2014,40(1):128-130.
[14]Zhao L M.The establishment of the molecular identification,complete genome sequence analysis,and detection technique of Tomato chlorosis virus(in Chinese)[D].Tai'an:Shandong Agricultural University(泰安:山东农业大学),2015.
[15]Wang C L,Feng J,Sun X H,et al.Molecular identification of Tomato chlorosis virus from four provinces or autonomous region in northern China(in Chinese)[J].Plant Protection(植物保护),2017,43(2):141-145.
[16]Hu J A,Wan X J,Li Z J,et al.Molecular identification of Tomato chlorosis virus in Henan Province(in Chinese)[J].China Vegetables(中国蔬菜),2015(12):25-28.
[17]Fondong V N,Pita J S,Rey M E C,et al.Evidence of synergism betw een African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon[J].Journal of General Virology,2000,81:287-297.
[18]Tatineni S,Graybosch R A,Hein G L,et al.Wheat cultivar-specific disease synergism and alteration of virus accumulation during co-infection w ith Wheat streak mosaic virus and Triticum mosaic virus[J].Phytopathology,2010,100(3):230-238.
[19]Srinivasan R,Alvarez J M.Effect of mixed viral infections(Potato virus Y-Potato leafroll virus)on biology and preference of vectors Myzus persicae and Macrosiphum euphorbiae(Hemiptera;Aphididae)[J].Journal of Economic Entomology,2007,100(3):646-655.