高脂饮食对兔睾丸组织损害机制及光甘草定保护作用研究
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摘要
目的研究光甘草定(GLA)对高脂模型兔睾丸功能的保护作用及机制。方法建立高脂模型兔模型,随机将作为模型兔的新西兰兔分成三组,分别为光甘草定组、高脂模型组以及正常对照组。其中,以高脂饲料喂养高脂组与GLA组,以正常饲料方式喂养正常对照组,持续12周;GLA组自第7周开始给予GLA,剂量2 mg/(kg·d),至第12周。分析血脂总胆固醇(TCH)和三酰甘油(TG)变化;免疫组织化学法研究睾丸间质细胞和巨噬细胞的变化;脂质氧化(MDA)检测和总抗氧化能检测试剂盒(ABTS快速法)分别检测睾丸组织脂质氧化水平和总抗氧化能力;Western blot分析睾酮合成相关蛋白/细胞色素P450胆固醇侧链裂解酶(P450 scc)和类固醇合成快速调节蛋白(StAR)、抗氧化蛋白沉默信息调节因子2相关酶1(SIRT1)、锰超氧化物歧化酶(MnSOD)、人谷脱甘肽过氧化酶4(GPx4)及内质网应激标记分子葡萄糖调节蛋白78(GRP78)表达。结果与正常对照组相比,高脂组血清TCH增高(P<0.05);睾丸间质细胞表达3β-羟类固醇脱氢酶(3β-HSD)水平降低;多克隆抗体(F4/80)标记巨噬细胞数量增加;Western blot示P450 scc、StAR、SIRT1、GPx4和MnSOD表达降低(P<0.05);机体脂质氧化增加,总抗氧化能力减弱(P>0.05);GRP78表达上调;高脂添加GLA组与高脂组相比,TCH降低(P<0.05);睾丸间质细胞3β-HSD表达增强;巨噬细胞数量减少;P450 scc、StAR、SIRT1、GPx4和MnSOD表达升高(P<0.05);脂质氧化减少,总抗氧化能力增强;GRP78表达有下降趋势。结论 GLA可以减弱高脂对兔睾丸间质细胞睾酮合成能力的损害,其抗氧化应激作用可能是重要机制。
Objective To study the protective effect and mechanism of glabridin on the testis function of high fat model rabbits. Methods To establish a high-fat rabbit model, the rabbits were randomly divided into three groups:normal control group, high-fat model group, and glabridin group. Normal feed feeding in the control group; the high-fat group and the glabridin group were fed with high-fat feed for 12 weeks. The glabridin group was fed with glabridin from the 7 th week to 12 th week; the dose was 2 mg/(kg·d). The changes of total cholesterol and triglycerides were analyzed. Study on the changes of macrophages in Leydig cells were tested by immunohistochemistry; Western blot was used to analyze the testosterone synthesis related protein/enzyme P450 scc and StAR expression, antioxidant protein/enzyme SIRT1, MnSOD, GPx4 and endoplasmic reticulum stress marker GRP78 expression; MDA and ABTS kit were used to test the lipid oxidation level and total antioxidant capacity of testicular tissue. Results Compared with the control group, serum total cholesterol(P<0.05) was increased in the high-fat group. The expression 3β-HSD level in Leydig cells was decreased; F4/80 antibody labeled macrophages increased in number; Western blot showed a decrease in expression of P450 scc,StA R,SIRT1,GPx4 and MnSOD( P <0. 05). The body lipid oxidation increased and the total antioxidant capacity decreased( P > 0. 05). GRP78 expression was up-regulated; The total cholesterol decreased( P < 0. 05) compared with that in the high-fat group.Leydig cell 3β-HSD expression enhancement; the number of macrophages decreased; the expression of P450 scc,StAR,SIRT1,GPx4 and MnSOD increased( P < 0. 05). Decreased lipid oxidation and total antioxidant capacity enhanced( P > 0. 05); The expression of GRP78 showed a downward trend. Conclusion Glabridin can reduce the damage of high fat on the synthesis of testosterone in rabbit testis,and its anti-oxidative stress might be an important mechanism.
Objective To study the protective effect and mechanism of glabridin on the testis function of high fat model rabbits. Methods To establish a high-fat rabbit model, the rabbits were randomly divided into three groups:normal control group, high-fat model group, and glabridin group. Normal feed feeding in the control group; the high-fat group and the glabridin group were fed with high-fat feed for 12 weeks. The glabridin group was fed with glabridin from the 7 th week to 12 th week; the dose was 2 mg/(kg·d). The changes of total cholesterol and triglycerides were analyzed. Study on the changes of macrophages in Leydig cells were tested by immunohistochemistry; Western blot was used to analyze the testosterone synthesis related protein/enzyme P450 scc and StAR expression, antioxidant protein/enzyme SIRT1, MnSOD, GPx4 and endoplasmic reticulum stress marker GRP78 expression; MDA and ABTS kit were used to test the lipid oxidation level and total antioxidant capacity of testicular tissue. Results Compared with the control group, serum total cholesterol(P<0.05) was increased in the high-fat group. The expression 3β-HSD level in Leydig cells was decreased; F4/80 antibody labeled macrophages increased in number; Western blot showed a decrease in expression of P450 scc,StA R,SIRT1,GPx4 and MnSOD( P <0. 05). The body lipid oxidation increased and the total antioxidant capacity decreased( P > 0. 05). GRP78 expression was up-regulated; The total cholesterol decreased( P < 0. 05) compared with that in the high-fat group.Leydig cell 3β-HSD expression enhancement; the number of macrophages decreased; the expression of P450 scc,StAR,SIRT1,GPx4 and MnSOD increased( P < 0. 05). Decreased lipid oxidation and total antioxidant capacity enhanced( P > 0. 05); The expression of GRP78 showed a downward trend. Conclusion Glabridin can reduce the damage of high fat on the synthesis of testosterone in rabbit testis,and its anti-oxidative stress might be an important mechanism.
引文
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[2] Gupta V K,Fatima A,Faridi U,et al.Antimicrobal potential of Glycyrrhiza glabra roots [J].Ethnopharmacol,2008,116(2):377-80.
[3] Cheema H S,Prakash O,Pal A,et al.Glabridin induces oxidative stress mediated apoptosis like cell death of malaria parasite plasmodium falciparum[J].Parasitol Int,2014,63(2):349-58.
[4] Singh V,Pal A,Darokar M P,et al.A polyphenolic flavonoid glabridin:oxidative stress response in multidrug-resistant staphylococcus aureus [J].Free Radic Bio Med,2015,87:48-57.
[5] Ye X,Jiang F,Li Y,et al.Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c[J].Cancer Sci,2014,105(7):875-82.
[6] Lee J W,Choe S S,Jang H,et al.AMPK activation with glabridin ameliorates adiposity and lipid dysregulation in obesity [J].J Lipid Res,2012,53(7):1277-86.
[7] 王淦娴,王怡,丁俊丽,等.光甘草定通过p38-MAPK通路调控动脉粥样硬化模型兔动脉MLCK表达[J].安徽医科大学学报,2017,52(3):309-13.
[8] Lv Z M,Wang Q,Chen Y H,et al.Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue:implications for its involvement in improving steroidogenesis in diet-induced obese mice [J].Mol Reprod Dev,2015,82(4):321-8.
[9] Erdemir F,Atilgan D,Markoc F,et al.The effect of diet induced obesity on testicular tissue and serum oxidative stress parameters[J].Actas Urol Esp,2012,36(3):153-9.
[10] Mancini A,Festa R,Raimondo S,et al.Biochemical alterations in semen of varicocele patients:a review of the literature[J].Adv Urol,2012,2012:903931.
[11] Chen H,Guo J,Ge R,et al.Steroidogenic fate of the Leydig cells that repopulate the testes of young and aged Brown Norway rats after elimination of the preexisting Leydig cells[J].Exp Gerontol,2015,72:8-15.
[12] Tsai Y C,Weissman A M.The unfolded protein response,degradation from the endoplasmic reticulum and cancer[J].Genes Cancer,2010,1(7):764-78.
[13] Cao S S,Kaufman R J.Endoplasmic reticulum stress and oxidative stress in cell fate decision and human disease[J].Antioxid Redox Signal,2014,21(3):396-413.
[14] Hotamisligil G S.Endoplasmic reticulum stress and the inflammatory basis of metabolic disease[J].Cell,2010,140(6):900-17.
[15] Brown M K,Naidoo N,et al.The endoplasmic reticulum stress response in aging and age-related diseases[J].Front Physiol,2012,3:263.