南极磷虾油中砷甜菜碱在大鼠体内的代谢
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摘要
为研究南极磷虾(Euphausia superba)油中的砷甜菜碱在大鼠体内的吸收、分布和蓄积情况,随机将雄性Wistar大鼠分为3组:大豆油对照组、磷虾油组、添加100 mg/kg砷甜菜碱的磷虾油组,灌胃剂量为2.625 g油/kg·bw。于灌胃后1、2、4、6、12、24h,经戊巴比妥钠麻醉,腹主动脉取血处死大鼠,并收集12 h和24 h尿液,ICP-MS法检测血浆、肝脏、肾脏、脾和尿液中总砷含量。另连续灌胃同等剂量油脂7 d和30 d,检测肝脏和肾脏中总砷含量。结果显示,磷虾油组和对照组大鼠血浆和脏器中总砷含量各时间点无显著变化;砷甜菜碱磷虾油组大鼠血浆中总砷含量于1 h达到最高,脏器中总砷含量均在6 h达到最高,24 h后降低至最高值的30%以下;85%的砷会在24 h之内随尿液排出体外。7 d和30 d连续灌胃后,2个实验组大鼠脏器中总砷含量较对照组均无显著变化。南极磷虾油中的砷甜菜碱在大鼠体内很少蓄积,大部分于24h内随尿液排出体外,而长期摄食也未出现脏器蓄积现象。本研究对于指导南极磷虾油及其相关产品的科学消费和拓展应用具有重要意义。
To study the absorption, distribution, and accumulation of arsenobetaine in Antarctic krill(Euphausia superba) oil, Wistar rats were randomly divided into 3 groups and treated with 2.625 g/kg·bw of soybean oil, krill oil, or 100 mg/kg arsenobetaine added to krill oil. The plasma, liver, kidney and spleen of the rats were collected at 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h after gavage, whereas the urine was also collected at 12 h and 24 h. The total arsenic content in the plasma, organs, and urine was detected by ICP-MS. The total arsenic contents in liver and kidney of rats were also measured at 7 d and 30 d after continuous gavage with the same dose of the lipids. The total arsenic contents in the plasma and organs showed no obvious changes at each time point between the krill oil group and the control group. The maximum content of total arsenic in plasma occurred at 1 h after gavage, whereas the total arsenic content in the liver, kidney, and spleen were all the highest at 6 h after gavage. At 24 h after gavage, the content of total arsenic in the liver, kidney, and spleen decreased to below 30% of the maximum. Within 24 h, 85% of the arsenic in the krill oil was passed out of the body in the urine. After continuous gavage for 7 days and 30 days, the total arsenic contents in the liver and kidney of the rats in each group showed no significant changes when compared with those of the control group. Arsenobetaine in the krill oil was rarely absorbed in rats and most passed out of the body in the urine within 24 h. No cumulative effects appeared after long-term feeding with the krill oil and the arsenobetaine.
To study the absorption, distribution, and accumulation of arsenobetaine in Antarctic krill(Euphausia superba) oil, Wistar rats were randomly divided into 3 groups and treated with 2.625 g/kg·bw of soybean oil, krill oil, or 100 mg/kg arsenobetaine added to krill oil. The plasma, liver, kidney and spleen of the rats were collected at 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h after gavage, whereas the urine was also collected at 12 h and 24 h. The total arsenic content in the plasma, organs, and urine was detected by ICP-MS. The total arsenic contents in liver and kidney of rats were also measured at 7 d and 30 d after continuous gavage with the same dose of the lipids. The total arsenic contents in the plasma and organs showed no obvious changes at each time point between the krill oil group and the control group. The maximum content of total arsenic in plasma occurred at 1 h after gavage, whereas the total arsenic content in the liver, kidney, and spleen were all the highest at 6 h after gavage. At 24 h after gavage, the content of total arsenic in the liver, kidney, and spleen decreased to below 30% of the maximum. Within 24 h, 85% of the arsenic in the krill oil was passed out of the body in the urine. After continuous gavage for 7 days and 30 days, the total arsenic contents in the liver and kidney of the rats in each group showed no significant changes when compared with those of the control group. Arsenobetaine in the krill oil was rarely absorbed in rats and most passed out of the body in the urine within 24 h. No cumulative effects appeared after long-term feeding with the krill oil and the arsenobetaine.
引文
Chen FH,Zhang XR.Arsenobetaine in marine animals.Marine Environmental Science,1993,12(2):58-67[陈甫华,张相如.海洋动物中的砷甜菜碱.海洋环境科学,1993,12(2):58-67]
Dopp E,Kligerman AD,Diazbone RA.Organoarsenicals.Uptake,metabolism,and toxicity.Metal Ions in Life Sciences,2010,7:231
He RK,Luo HJ,et al.Health benefits of Antarctic krill oil.Food Research and Development,2013,34(20):130-133
Kaise T,Horiguchi Y,Fukui S,et al.Acute toxicity and metabolism of arsenocholine in mice.Applied Organometallic Chemistry,1992,6(4):369-373
Khairul I,Wang QQ,Jiang YH,et al.Metabolism,toxicity and anticancer activities of arsenic compounds.Oncotarget,2017,8(14):23905-23926
Liu JJ,Feng DN,Su XF,et al.Optimization of formula for Antartic krill oil soft capsules shell.Progress in Fishery Sciences,2014,35(4):141-144[刘建君,冯迪娜,苏学锋,等.南极磷虾油软胶囊囊皮配方的设计与优化.渔业科学进展,2014,35(4):141-144]
Liu Q,Huang HL,Li LN et al.Strategic thinking behind the commercial development of Antarctic krill.Chinese Journal of Polar Research,2015(1):31-37[刘勤,黄洪亮,李励年,等.南极磷虾商业化开发的战略性思考.极地研究,2015(1):31-37]
Liu Q,Liu ZD,Lu YN,et al.On Antarctic krill products and development trends.Morden Fisheries Information,2014,29(2):115-21[刘勤,刘志东,陆亚男,等.南极磷虾产品研究及发展趋势.渔业信息与战略,2014,29(2):115-121]
Liu Y,Wu J.Advances in research on the metabolism of arsenic in the liver and its effects on hepatic biochemical parameters.World Chinese Journal of Digestology,2009,17(34):3524-3529[刘洋,吴君.砷在肝脏中的代谢及对其生化指标影响的研究进展.世界华人消化杂志,2009,17(34):3524-3529]
Li W,Li B.Health effects of long-time arsenic exposure:Areview of recent studies.Journal of Environment and Health,2016,33(9):836-841[李伟,李冰.环境砷暴露对机体损伤的研究进展.环境与健康杂志,2016,33(9):836-841]
Lu HFS,Bruheim I,Jacobsen C,et al.Oxidative stability and non-enzymatic browning reactions in Antarctic krill oil(Euphausia superba).Lipid Technology,2014,26(5):111-114
Molin M,Ulven SM,Meltzer HM,et al.Arsenic in the human food chain,biotransformation and toxicology-Review focusing on seafood arsenic.Journal of Trace Elements in Medicine and Biology Organ of the Society for Minerals&Trace Elements,2015,31:249
Nearing MM,Koch I,Reimer KJ.Complementary arsenic speciation methods:A review.Spectrochimica Acta Part BAtomic Spectroscopy,2014,99(9):150-162
Nie YC,Zhang B,Zhao XY,et al.Seasonal variation in lipids and protein content of Antarctic krill(Euphausia superba).Progress in Fishery Sciences,2016,37(3):1-8[聂玉晨,张波,赵宪勇,等.南极磷虾(Euphausia superba)脂肪与蛋白含量的季节变化.渔业科学进展,2016,37(3):1-8]
Sadee B,Foulkes ME,Hill SJ.Coupled techniques for arsenic speciation in food and drinking water:A review.Journal of Analytical Atomic Spectrometry,2015,30(1):102-118
Taylor V,Goodale B,Raab A,et al.Human exposure to organic arsenic species from seafood.Science of the Total Environment,2016,580:266-282
Wang S,Li K,Cui H et al.Quantitative analysis of total and different species of arsenic in oil of Antarctic krill(Euphausia superba).Chinese Journal of Analytical Chemistry,2016,44(5):767-772[王松,Li K,崔鹤,等.南极磷虾油中总砷含量及砷形态分析.分析化学,2016,44(5):767-772]
Wang YE,Jiang GL,Xu K,et al.Effects of the Antarctic krill oil on the serum lipids and antioxidation ability in hyperlipidemia rats.Chinese Journal of Marine Drugs,2011,30(3):56-59
Yang RY,Li ZJ,Lin Q,et al.Distribution of arsenic in arsenic-poisoning rat organs.Chinese Journal of Control of Endemic Diseases,2005,20(5):260-262[杨瑞瑛,李子杰,林勤,等.砷中毒大鼠体内各脏器、组织中砷的分布.中国地方病防治杂志,2005,20(5):260-262]
Zhou DY.Effects of long-term intake of Antarctic krill oils on artery blood pressure in spontaneously hypertensive rats.Journal of the Science of Food&Agriculture,2016,97(4):
Zhou Y,He LP,Huang X,et al.Immunological function among people exposed to lead,cadmium,and arsenic pollution.Chinese Journal of Public Health,2010,26(2):243-244[周勇,贺莉萍,黄晓,等.铅、镉、砷污染地区人群免疫功能检测分析.中国公共卫生,2010,26(2):243-244]
Dopp E,Kligerman AD,Diazbone RA.Organoarsenicals.Uptake,metabolism,and toxicity.Metal Ions in Life Sciences,2010,7:231
He RK,Luo HJ,et al.Health benefits of Antarctic krill oil.Food Research and Development,2013,34(20):130-133
Kaise T,Horiguchi Y,Fukui S,et al.Acute toxicity and metabolism of arsenocholine in mice.Applied Organometallic Chemistry,1992,6(4):369-373
Khairul I,Wang QQ,Jiang YH,et al.Metabolism,toxicity and anticancer activities of arsenic compounds.Oncotarget,2017,8(14):23905-23926
Liu JJ,Feng DN,Su XF,et al.Optimization of formula for Antartic krill oil soft capsules shell.Progress in Fishery Sciences,2014,35(4):141-144[刘建君,冯迪娜,苏学锋,等.南极磷虾油软胶囊囊皮配方的设计与优化.渔业科学进展,2014,35(4):141-144]
Liu Q,Huang HL,Li LN et al.Strategic thinking behind the commercial development of Antarctic krill.Chinese Journal of Polar Research,2015(1):31-37[刘勤,黄洪亮,李励年,等.南极磷虾商业化开发的战略性思考.极地研究,2015(1):31-37]
Liu Q,Liu ZD,Lu YN,et al.On Antarctic krill products and development trends.Morden Fisheries Information,2014,29(2):115-21[刘勤,刘志东,陆亚男,等.南极磷虾产品研究及发展趋势.渔业信息与战略,2014,29(2):115-121]
Liu Y,Wu J.Advances in research on the metabolism of arsenic in the liver and its effects on hepatic biochemical parameters.World Chinese Journal of Digestology,2009,17(34):3524-3529[刘洋,吴君.砷在肝脏中的代谢及对其生化指标影响的研究进展.世界华人消化杂志,2009,17(34):3524-3529]
Li W,Li B.Health effects of long-time arsenic exposure:Areview of recent studies.Journal of Environment and Health,2016,33(9):836-841[李伟,李冰.环境砷暴露对机体损伤的研究进展.环境与健康杂志,2016,33(9):836-841]
Lu HFS,Bruheim I,Jacobsen C,et al.Oxidative stability and non-enzymatic browning reactions in Antarctic krill oil(Euphausia superba).Lipid Technology,2014,26(5):111-114
Molin M,Ulven SM,Meltzer HM,et al.Arsenic in the human food chain,biotransformation and toxicology-Review focusing on seafood arsenic.Journal of Trace Elements in Medicine and Biology Organ of the Society for Minerals&Trace Elements,2015,31:249
Nearing MM,Koch I,Reimer KJ.Complementary arsenic speciation methods:A review.Spectrochimica Acta Part BAtomic Spectroscopy,2014,99(9):150-162
Nie YC,Zhang B,Zhao XY,et al.Seasonal variation in lipids and protein content of Antarctic krill(Euphausia superba).Progress in Fishery Sciences,2016,37(3):1-8[聂玉晨,张波,赵宪勇,等.南极磷虾(Euphausia superba)脂肪与蛋白含量的季节变化.渔业科学进展,2016,37(3):1-8]
Sadee B,Foulkes ME,Hill SJ.Coupled techniques for arsenic speciation in food and drinking water:A review.Journal of Analytical Atomic Spectrometry,2015,30(1):102-118
Taylor V,Goodale B,Raab A,et al.Human exposure to organic arsenic species from seafood.Science of the Total Environment,2016,580:266-282
Wang S,Li K,Cui H et al.Quantitative analysis of total and different species of arsenic in oil of Antarctic krill(Euphausia superba).Chinese Journal of Analytical Chemistry,2016,44(5):767-772[王松,Li K,崔鹤,等.南极磷虾油中总砷含量及砷形态分析.分析化学,2016,44(5):767-772]
Wang YE,Jiang GL,Xu K,et al.Effects of the Antarctic krill oil on the serum lipids and antioxidation ability in hyperlipidemia rats.Chinese Journal of Marine Drugs,2011,30(3):56-59
Yang RY,Li ZJ,Lin Q,et al.Distribution of arsenic in arsenic-poisoning rat organs.Chinese Journal of Control of Endemic Diseases,2005,20(5):260-262[杨瑞瑛,李子杰,林勤,等.砷中毒大鼠体内各脏器、组织中砷的分布.中国地方病防治杂志,2005,20(5):260-262]
Zhou DY.Effects of long-term intake of Antarctic krill oils on artery blood pressure in spontaneously hypertensive rats.Journal of the Science of Food&Agriculture,2016,97(4):
Zhou Y,He LP,Huang X,et al.Immunological function among people exposed to lead,cadmium,and arsenic pollution.Chinese Journal of Public Health,2010,26(2):243-244[周勇,贺莉萍,黄晓,等.铅、镉、砷污染地区人群免疫功能检测分析.中国公共卫生,2010,26(2):243-244]
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