沙葱水提液体外抗氧化及α-葡萄糖苷酶与胰脂肪酶抑制作用的研究
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摘要
以新鲜沙葱为原料,用纯水对其进行提取,并且以该取提液为研究对象,测定其中的黄酮含量,通过测定1,1-二苯基-2-苦肼基自由基(DPPH·)清除能力来评价沙葱水提液的体外抗氧化活性,并分别采用α-葡萄糖苷酶与胰脂肪酶抑制模型研究其对α-葡萄糖苷酶和胰脂肪酶活性的抑制作用。结果显示:沙葱中总黄酮含量为4.02 mg QE/g DW,对DPPH自由基有显著的清除活性,测定得到的Trolox等价抗氧化能力为51.82 mg Trolox/g,半抑制浓度(half maximal inhibitory concentration,IC50)为0.867mg/mL,对α-葡萄糖苷酶和胰脂肪酶活性抑制的IC_(50)值分别为326.29mg/mL和179.48 mg/mL,且都呈明显的质量浓度依赖关系。结果表明,沙葱中黄酮含量丰富,有显著的体外抗氧化活性,并且有良好的α-葡萄糖苷酶与胰脂肪酶抑制活性,有深入开发研究的价值。
The Allium mongolicum Regel(AM)was used as raw material and extracted by pure water,and the total flavonoid content(TFC)was quantified.The antioxidant capacity of the aqueous extract was evaluated by testing the scavenging activity on 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·).Furthermore,inhibitory effects against α-glucosidase and pancreatic lipase in vitro of AM aqueous extract were assessed.Results showed that the TFC of AM was 4.02 mg QE/g DW and could scavenge DPPH radical effectively with IC50 of 0.867 mg/mL,the Trolox equivalent antioxidant capacity of DPPH assay was 51.82 mg Trolox/g DW.It also showed inhibitory activity against α-glucosidase and pancreatic lipase with IC_(50) were 326.29 mg/mL and179.48 mg/mL respectively,and both exhibited in a concentration manner.In summary,the flavonoid-rich AM had strong antioxidant potential and well inhibitory effects against α-glucosidase and pancreatic lipase,which deserves further research and development.
The Allium mongolicum Regel(AM)was used as raw material and extracted by pure water,and the total flavonoid content(TFC)was quantified.The antioxidant capacity of the aqueous extract was evaluated by testing the scavenging activity on 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·).Furthermore,inhibitory effects against α-glucosidase and pancreatic lipase in vitro of AM aqueous extract were assessed.Results showed that the TFC of AM was 4.02 mg QE/g DW and could scavenge DPPH radical effectively with IC50 of 0.867 mg/mL,the Trolox equivalent antioxidant capacity of DPPH assay was 51.82 mg Trolox/g DW.It also showed inhibitory activity against α-glucosidase and pancreatic lipase with IC_(50) were 326.29 mg/mL and179.48 mg/mL respectively,and both exhibited in a concentration manner.In summary,the flavonoid-rich AM had strong antioxidant potential and well inhibitory effects against α-glucosidase and pancreatic lipase,which deserves further research and development.
引文
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[23]Oboh G,Ademiluyi A O,Agunloye O M,et al.Inhibitory Effect of Garlic,Purple Onion,and White Onion on Key Enzymes Linked with Type 2 Diabetes and Hypertension[J].Journal of Dietary Supplements,2018,6(15):1-14
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[25]李恒彪,曾斌,向阳,等.洋葱辅助调脂减肥功效研究[J].食品研究与开发,2014,35(5):10-12
[2]Muqier,Sarula Q,Terigele W,et al.Effects of flavonoids from Allium mongolicum Regel on growth performance and growth-related hormones in meat sheep[J].Animal Nutrition,2017,3:33-38
[3]马毓泉.内蒙古植物志(第5卷)[M].第二版.呼和浩特:内蒙古人民出版社,1994:489-491
[4]黄晴,吴忠坤,吴中琴,等.葱属类植物中有机硫化物的抗氧化性研究进展[J].食品研究与开发,2018,39(1):214-220
[5]Zhang T Y,Chen H K,Zhang C R.Polymorphic microsatellite markers for Allium mongolicum Regal(Amaryllidaceae)[J].Genes Genetic Systems,2014,89(3):133-136
[6]张璐,郑亚军,李艳.槟榔籽乙醇提取物抗氧化性的研究[J].食品研究与开发,2016,37(8):1-4
[7]Hsieh J F,Lin W J,Huang K F,et al.Antioxidant activity and inhibition ofα-glucosidase by hydroxyl-functionalized 2-arylbenzo[b]furans[J].European Journal of Medicine Chemistry,2015,93:443-451
[8]Kim G N,Shin M R,Shin S H,et al.Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity of Diospyros kaki Fruit and Citrus unshiu Peel[J].Biomed Research International,2016,2016:1-7
[9]赵春艳,敖长金,缪亚娟,等.沙葱黄酮对小鼠抗氧化能力的影响[J].饲料工业,2009,30(24):10-13
[10]Xin H,Qi X Y,Wu J J,et al.Assessment of the inhibition potential of Licochalcone A against human UDP-glucuronosyl transferases[J].Food and Chemical Toxicology,2016,90:112-122
[11]Kaska A,Deniz N,Cicek M,et al.Evaluation of Antioxidant Properties,Phenolic Compounds,Anthelmintic,and Cytotoxic Activities of Various Extracts Isolated from Nepeta cadmea:An Endemic Plant for Turkey[J].Journal of Food Science,2018,83(6):1552-1559
[12]Lopez-Yerena A,Guerra-Ramirez D,Jacome-Rincon J,et al.Initial evaluation of fruit of accessions of Persea schiedeana Nees for nutritional value,quality and oil extraction[J].Food Chemistry,2018,245:879-884
[13]Kang W Y,Song Y L,Zhang L.α-Glucosidase inhibitory and antioxidant properties and antidiabetic activity of Hypericum ascyron L.[J].Medicinal Chemistry Research,2010,20(7):809-816
[14]Hung W C,Ling X H,Chang C C,et al.Inhibitory Effects of Siegesbeckia orientalis Extracts on Advanced Glycation End Product Formation and Key Enzymes Related to Metabolic Syndrome[J].Molecules,2017,22(10):E1785
[15]姚文红,李飞阳,王娟,等.女贞花总黄酮提取及清除DPPH自由基作用的研究[J].食品研究与开发,2016,37(14):42-45
[16]赖小燕,姜泽东,倪辉,等.茶花粉黄酮对α-葡萄糖苷酶抑制作用的研究[J].食品工业科技,2016,37(5):353-357
[17]刘蕊,郑毅男,刘文丛.人参皂苷Rb3对胰脂肪酶的抑制作用[J].天然产物研究与开发,2011,23(3):522-525
[18]萨茹丽,木其尔,王翠芳,等.沙葱总黄酮提取工艺优化及其体外抗氧化、抗菌作用[J].食品科学,2014,35(24):1-8
[19]李富华,刘冬,明建.苦荞麸皮黄酮抗氧化及抗肿瘤活性[J].食品科学,2014,35(7):58-63
[20]邓浩,张容鹄,梁振益,等.木奶果花色苷提取纯化及其抗氧化活性研究[J].食品研究与开发,2016,37(21):32-36
[21]Wen H X,Tang B W,Stewart A J,et al.Erythritol Attenuates Postprandial Blood Glucose by Inhibitingα-Glucosidase[J].Journal of Agriculture and Food Chemistry,2018,66(6):1401-1407
[22]李波,包怡红,高锋,等.红松松球鳞片多酚对α-淀粉酶和α-葡萄糖苷酶的抑制作用[J].食品工业科技,2015,36(1):63-65
[23]Oboh G,Ademiluyi A O,Agunloye O M,et al.Inhibitory Effect of Garlic,Purple Onion,and White Onion on Key Enzymes Linked with Type 2 Diabetes and Hypertension[J].Journal of Dietary Supplements,2018,6(15):1-14
[24]Jaradat N,Zaid A N,Hussein F,et al.Anti-Lipase Potential of the Organic and Aqueous Extracts of Ten Traditional Edible and Medicinal Plants in Palestine;a Comparison Study with Orlistat[J].Medicines(Basel),2014,4(4):89-102
[25]李恒彪,曾斌,向阳,等.洋葱辅助调脂减肥功效研究[J].食品研究与开发,2014,35(5):10-12