正交试验法优化刺玫果总黄酮提取工艺
|
摘要
目的:为使刺玫果总黄酮更高效的提取出来,通过正交试验设计对刺玫果总黄酮提取工艺进行优化。方法:提取刺玫果总黄酮,采用正交试验、考察料液比、乙醇浓度、提取时间、提取次数对刺玫果总黄酮提取效果的影响,将总黄酮含量视为考察指标,选取最优提取工艺。结果:正交试验优化后提取工艺为:料液比1:10、醇浓度55%、提取时间2h、提取次数2次,总黄酮含量:27.41%。结论:由上述数据判断,正交试验实验次数少,数据点分布均匀,结论较可靠。
In order to extract the total flavonoids of Rosa davurica Pall more efficiently,orthogonal design was used to optimize the extraction process of total flavonoids from Rosa davurica Pall.With the yield of total flavonoids,the factors including the ratio of liquor to material,ethanol concentration,extraction time and repeated time were investigated by means of orthogonal design and response surface methodology,respectively.The following optimal extraction conditions were identified by using orthogonal design:the solid-liquid ratio,1:10(g·mL~(-1));the ethanol concentration,55%;extraction time,2h;repeated time,2;the total flavonoids yield reached 27.41%.Judging from the above data,Orthogonal experiments have fewer experiments and the data points are evenly distributed.The conclusion is more reliable.
In order to extract the total flavonoids of Rosa davurica Pall more efficiently,orthogonal design was used to optimize the extraction process of total flavonoids from Rosa davurica Pall.With the yield of total flavonoids,the factors including the ratio of liquor to material,ethanol concentration,extraction time and repeated time were investigated by means of orthogonal design and response surface methodology,respectively.The following optimal extraction conditions were identified by using orthogonal design:the solid-liquid ratio,1:10(g·mL~(-1));the ethanol concentration,55%;extraction time,2h;repeated time,2;the total flavonoids yield reached 27.41%.Judging from the above data,Orthogonal experiments have fewer experiments and the data points are evenly distributed.The conclusion is more reliable.
引文
[1]山西植物志编委会.山西植物志[M].北京:中国科学技术出版社,1998:335.
[2]王领弟,李艳荣,潘海峰,等.不同产地刺玫果中总黄酮含量的测定[J].承德医学院学报,2012,29(1):5-7.
[3]何媛媛,陈凡,孙爱东.刺玫果功效及食品开发研究进展[J].中国食物与营养,2015,21(6):25-28.
[4]程振伟,宋鑫秀,谢春阳.刺玫果果醋发酵工艺的研究[J].农产品加工(学刊),2014,(3):26-27;29.
[5]冯杉杉,金哲雄.刺玫果研究进展[J].黑龙江医药,2014,27(4):785-788.
[6]崔凤侠,王领弟,杜义龙,等.刺玫果提取物指纹图谱构建及4种成分含量测定[J].中国实验方剂学杂志,2014,20(7):93-96.
[7]俞作仁,王文莉,吕娟涛.刺玫果化学成分及药理作用研究进展[J].中草药,2002,(2):94-96.
[8]王领弟.山玫胶囊全过程质量控制研究[D].承德医学院,2012.
[9]朱琳,曾晓丹,张福胜.刺玫果中微量元素的测定[J].光谱实验室,2013,30(4):1656-1659.
[10]钟方丽,王晓林,杨扬.刺玫果提取物中总三萜酸的定性定量分析研究[J].林产化学与工业,2015,35(5):135-142.
[11]王晓林.高效液相色谱法测定刺玫果中槲皮素的含量[J].食品工业科技,2011,32(4):370-372.
[12]郝龙平,晏嘉泽,靳艳,等.刺玫果多糖的制备及其抗氧化活性[J].中成药,2016,38(12):2692-2696.
[13]钟方丽,王晓林,薛健飞,等.微波辅助提取刺玫果果胶工艺研究[J].食品工业科技,2015,36(11):239-242;246.
[14]杨扬,王晓林,薛健飞,等.刺玫果提取物中没食子酸的含量测定[J].食品工业,2015,36(1):284-286.
[15]郭向红,张鹏,匡海学,等.刺玫果口服液中没食子酸甲酯葡萄糖苷的含量测定[J].中医药信息,1999,16(3):53-54.
[16]Kim H M,Park Y A,Lee E J,et al.Inhibition of immediate-type allergic reaction by Rosa davurica Pall.in a murine model[J].Journal of Ethnopharmacology,1999,67(1):53.
[17]刘中申,李廷利,杨书彬,等.刺玫果浸膏粉对动脉粥样硬化及胆固醇影响的实验研究[J].中国老年学杂志,1998,(4):246-247.
[18]焦淑萍,陈彪,姜虹.山刺玫果实清除羟自由基及抗DNA损伤作用的实验研究[J].北华大学学报(自然科学版),2002,(4):307-308;318.
[19]金哲雄,齐典.刺玫果鞣质预防肿瘤作用研究[J].中华中医药学刊,2007,(4):647-648.
[20]韩竞,金哲雄.刺玫果的药理作用及临床应用概况[J].黑龙江医药,2014,27(1):106-108.
[21]高阳,姜会敏,霍雅玉,等.刺玫果的体外抗氧化活性研究[J].西北药学杂志,2014,29(3):226-230.
[22]Azahar N F,Gani S S A,Mokhtar N F M.Optimization of phenolics and flavonoids extraction conditions of Curcuma Zedoarialeaves using response surface methodology[J].Chemistry Central Journal,2017,11(1):96.
[2]王领弟,李艳荣,潘海峰,等.不同产地刺玫果中总黄酮含量的测定[J].承德医学院学报,2012,29(1):5-7.
[3]何媛媛,陈凡,孙爱东.刺玫果功效及食品开发研究进展[J].中国食物与营养,2015,21(6):25-28.
[4]程振伟,宋鑫秀,谢春阳.刺玫果果醋发酵工艺的研究[J].农产品加工(学刊),2014,(3):26-27;29.
[5]冯杉杉,金哲雄.刺玫果研究进展[J].黑龙江医药,2014,27(4):785-788.
[6]崔凤侠,王领弟,杜义龙,等.刺玫果提取物指纹图谱构建及4种成分含量测定[J].中国实验方剂学杂志,2014,20(7):93-96.
[7]俞作仁,王文莉,吕娟涛.刺玫果化学成分及药理作用研究进展[J].中草药,2002,(2):94-96.
[8]王领弟.山玫胶囊全过程质量控制研究[D].承德医学院,2012.
[9]朱琳,曾晓丹,张福胜.刺玫果中微量元素的测定[J].光谱实验室,2013,30(4):1656-1659.
[10]钟方丽,王晓林,杨扬.刺玫果提取物中总三萜酸的定性定量分析研究[J].林产化学与工业,2015,35(5):135-142.
[11]王晓林.高效液相色谱法测定刺玫果中槲皮素的含量[J].食品工业科技,2011,32(4):370-372.
[12]郝龙平,晏嘉泽,靳艳,等.刺玫果多糖的制备及其抗氧化活性[J].中成药,2016,38(12):2692-2696.
[13]钟方丽,王晓林,薛健飞,等.微波辅助提取刺玫果果胶工艺研究[J].食品工业科技,2015,36(11):239-242;246.
[14]杨扬,王晓林,薛健飞,等.刺玫果提取物中没食子酸的含量测定[J].食品工业,2015,36(1):284-286.
[15]郭向红,张鹏,匡海学,等.刺玫果口服液中没食子酸甲酯葡萄糖苷的含量测定[J].中医药信息,1999,16(3):53-54.
[16]Kim H M,Park Y A,Lee E J,et al.Inhibition of immediate-type allergic reaction by Rosa davurica Pall.in a murine model[J].Journal of Ethnopharmacology,1999,67(1):53.
[17]刘中申,李廷利,杨书彬,等.刺玫果浸膏粉对动脉粥样硬化及胆固醇影响的实验研究[J].中国老年学杂志,1998,(4):246-247.
[18]焦淑萍,陈彪,姜虹.山刺玫果实清除羟自由基及抗DNA损伤作用的实验研究[J].北华大学学报(自然科学版),2002,(4):307-308;318.
[19]金哲雄,齐典.刺玫果鞣质预防肿瘤作用研究[J].中华中医药学刊,2007,(4):647-648.
[20]韩竞,金哲雄.刺玫果的药理作用及临床应用概况[J].黑龙江医药,2014,27(1):106-108.
[21]高阳,姜会敏,霍雅玉,等.刺玫果的体外抗氧化活性研究[J].西北药学杂志,2014,29(3):226-230.
[22]Azahar N F,Gani S S A,Mokhtar N F M.Optimization of phenolics and flavonoids extraction conditions of Curcuma Zedoarialeaves using response surface methodology[J].Chemistry Central Journal,2017,11(1):96.