紫萼玉簪HvGASA、HvFAD基因的克隆及表达分析
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摘要
利用RACE技术、Genome Walking技术及PCR技术,从紫萼玉簪中克隆得到长度分别为336、1 320 bp的GASA基因HvGASA和脂肪酸去饱和酶基因HvFAD的cDNA全长序列,分别编码111、399个氨基酸,其中HvFAD基因所编码的蛋白为跨膜蛋白。qRT-PCR分析结果表明,2条基因随自然地温的降低均上调表达,而且在地温降至3℃时发生剧烈的表达量变化,说明HvGASA、HvFAD基因与紫萼玉簪的抗寒性具有密切关系。
引文
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[2]Herzog M,Dorne A M,Grellet F. GASA,a gibberellin-regulated gene family from Arabidopsis thaliana,related to the tomato GAST1 gene[J]. Plant Molecular Biology,1995,27(4):743-752.
[3]Furukawa T,Sakaguchi N,Shimada H. Two OsGASR genes,rice GAST homologue genes that are abundant in proliferating tissues,show different expression patterns in developing panicles[J]. Genes & Genetic Systems,2006,81(3):171-180.
[4]Ben-Nissan G,Lee J Y,Borohov A,et al. GIP,a petunia hybrida GA-induced cysteine-rich protein:a possible role in shoot elongation and transition to flowering[J]. Plant Journal,2004,37(2):229-238.
[5]Sun S L,Wang H X,Yu H M,et al. GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation[J]. Journal of Experimental Botany,2013,64(6):1637-1647.
[6]Wolf S,van der Does D,Ladwig F,et al. A receptor-like protein mediates the response to pectin modification by activating brassinosteroid signaling[J]. Proceedings of the National Academy of Sciences of the United States of America,2014,111(42):15261-15266.
[7]钟春梅,王小菁. 富含半胱氨酸的GASA小分子蛋白研究进展[J]. 植物学报,2016,51(1):1-8.
[8]Lyons J M. Chilling injury in plants[J]. Annual Review of Plant Physiology,1973,24(1):445-466.
[9]李飞,徐建飞,刘杰,等. 三个耐冻性不同的马铃薯野生种中FAD2基因的克隆及表达分析[J]. 作物学报,2014,40(1):45-53.
[10]Gigon A,Matos A R,Laffray D,et al. Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia)[J]. Annals of Botany,2004,94(3):345-351.
[11]Zhang M,Barg R,Yin M G,et al. Modulated fatty acid desaturation via overexpression of two distinct omega-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants[J]. Plant Journal,2005,44(3):361-371.
[12]Chaffai R,Elhammadi M A,Seybou T N,et al. Altered fatty acid profile of polar lipids in maize seedlings in response to excess copper[J]. Journal of Agronomy and Crop Science,2007,193(3):207-217.
[13]卢善发. 植物脂肪酸的生物合成与基因工程[J]. 植物学通报,2000,17(6):481-491.
[14]苏华楠,王雪峰,黄爱军,等. 高质量提取柑橘样品中病原总核酸方法的建立[J]. 园艺学报,2014,41(11):2342-2352.
[15]朱晓仙. 玉簪花中3个乙烯生物合成相关基因的克隆及其表达分析[D]. 金华:浙江师范大学,2012.
[16]冯延芝. 杜仲种仁转录组测序及FAD3基因的鉴定与功能研究[D]. 北京:中国林业科学研究院,2016.
[17]Ko C B,Woo Y M,Lee D J,et al. Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene[J]. Plant Physiology and Biochemistry,2007,45(9):722-728.
[18]张强,孟月娥,李艳敏,等. 玫瑰GASA4-like基因的克隆及其序列分析[J]. 河南农业科学,2009,38(9):161-166.
[19]McConn M,Hugly S,Browse J,et al. A mutation at the fad8 locus of Arabidopsis identifies a second chloroplast ω-3 desaturase[J]. Plant Physiology,1994,106(4):1609-1614.
[20]袁中厚. 紫苏ω-3脂肪酸脱氢酶基因的克隆与表达分析[D]. 重庆:重庆师范大学,2014.
[21]Somerville C. Direct tests of the role of membrane lipid composition in low-temperature-induced photoinhibition and chilling sensitivity in plants and cyanobacteria[J]. Proceedings of the National Academy of Sciences of the United States of America,1995,92(14):6215-6218.