家蚕胚胎发育过程的组织形态显微观察
|
摘要
胚胎发育是动物许多重要器官与性状发育的重要时期。本文利用石蜡切片法,对家蚕的胚胎发育进行了观察,并在显微镜可见光明场下拍照记录胚胎发育的形态特殊时期及其经历的发育时间,直观地获得家蚕胚胎发育完整过程。实验完整记录了家蚕胚胎发育的5个胚胎发育时期及其相对应:卵裂与胚盘形成期、胚带形成期、器官形成期和完成期及其相对应的发育时间。为进一步研究家蚕个体发育,器官的形成与分化奠定基础。
The Embryonic development is an important process of animals because extensive research into embryogenesis will improve our understanding of insect developmental mechanisms. To explore the embryonic development of silkworm(Bombyx mori), we used paraffin section technical method to detect morphological development from fertilization to hatching in the paper. Embryonic stages, syncytial cleavage, blastoderm, cellularization, germ rudiment condensation, germ band formation, head segmentation, abdominal segmentation, appendage segmentation, blastokinesis, completion of organs and hatching with the duration of each stage were recorded. This work will facilitate further studies on developmental biology of B. mori and silkworm grainage.
The Embryonic development is an important process of animals because extensive research into embryogenesis will improve our understanding of insect developmental mechanisms. To explore the embryonic development of silkworm(Bombyx mori), we used paraffin section technical method to detect morphological development from fertilization to hatching in the paper. Embryonic stages, syncytial cleavage, blastoderm, cellularization, germ rudiment condensation, germ band formation, head segmentation, abdominal segmentation, appendage segmentation, blastokinesis, completion of organs and hatching with the duration of each stage were recorded. This work will facilitate further studies on developmental biology of B. mori and silkworm grainage.
引文
Chai CL, Lu C.Comparison of early embryogenesis between silkworm and Drosophila from both morphology and gene content level [J]. Hereditas, 2006, 28 (9): 1173-1179. [柴春利, 鲁成. 从形态学不同到基因水平差异: 家蚕与果蝇早期胚胎发育比较 [J]. 遗传, 2006, 28 (9): 1173-1179]
Landi S, Gargani E, Paoli F,et al. Morphological markers for cryopreservation in the embryonic development of Drosophila suzukii (Diptera: Drosophilidae)[J]. J. Econ. Entomol., 2015, 108: 1875-1883.
Lee TY. The development of Trichogramma evanescens Westw. and its influence on the embryonic development of its host, Attacus cynthia ricini Boisd[J]. Acta Entomologica Sinica, 1961, 10 (4-6): 339-354, 525-530. [利翠英. 赤眼蜂Trichogramma evanescens Westw.的个体发育及其对于寄主蓖麻蚕Attacus cynthia ricini Boisd.胚胎发育的影响 [J]. 昆虫学报, 1961, 10 (4-6): 339-354, 525-530]
Li JY, Moghaddam SH, Chen JE,et al. Shotgun proteomic analysis on the embryos of silkworm Bombyx mori at the end of organogenesis [J]. Insect Biochem. Mol. Biol., 2010, 40: 293-302.
Liu MJ, Tong XL, Zhang GJ. Advances in researches of the mechanism of diapause in the silkworm,Bombyx mori [J]. News letter of Sericultureal Science, 2014: 23-31.[刘玫静, 童晓玲, 张高军. 家蚕滞育机理研究进展 [J]. 蚕学通讯, 2014: 23-31]
Lu HS. Principles of the Silkworm Grainage [M]. Shanghai: Shanghai Scientific & Technical Publishers, 2011: 72-83, 95-103. [吕鸿声, 蚕种学原理[M]. 上海: 上海科学技术出版社, 2011: 72-83, 95-103]
Nagy L, Riddiford L, Kiguchi K. Morphogenesis in the early embryo of the lepidopteran Bombyx mori [J]. Dev. Biol., 1994, 165: 137-151.
Nakagaki M, Takei R, Nagashima E,et al. Cell cycles in embryos of the silkworm, Bombyx mori: G2-arrest at diapause stage [J]. Roux Arch. Dev. Biol., 1990, 200: 223-229.
Pan MH, Xu W, Su MK,et al. Study on the morphology of embryonic development states in silkworm, Bombyx mori [J]. Canye Kexue, 2007: 667-73. [潘敏慧, 徐伟, 苏茂科, 等. 家蚕胚胎发育过程的组织形态学研究 [J]. 蚕业科学, 2007: 667-73]
Ponnuvel KM, Murthy GN, Awasthi AK,et al. Differential gene expression during early embryonic development in diapause and non-diapause eggs of multivoltine silkworm Bombyx mori [J]. Indian J. Exp. Biol., 2010, 48: 1143-1151.
Sasibhushan S, Rao CGP, Ponnuvel KM. Genome wide microarray based expression profiles during early embryogenesis in diapause induced and non-diapause eggs of polyvoltine silkworm Bombyx mori [J]. Genomics, 2013, 102: 379-387.
Steponkus PL, Myers SP, Lynch DV,et al. Cryopreservation of Drosophila melanogaster embryos [J]. Nature, 1990, 345: 170-172.
Tu ZL, Hong DZ, Zhao LH. Division and movement observation and digitalization analysis of Bombyx mori fertilized nuclei in early embryonic development [J]. Science of Sericulture, 2014: 254-57. [屠振力, 洪德志, 赵丽华. 家蚕胚胎发育初期受精核的分裂与移动观察及数值化分析 [J]. 蚕业科学, 2014: 254-57]
Xiang ZH. Biology of Sericulture [M]. Beijing: China Forestry Publishing House, 2005: 70-89. [向仲怀. 蚕丝生物学 [M]. 北京: 中国林业出版社, 2005: 70-89]
Xu W. The Study of Embryonie Development and Immunoloealization of BmMago Based on Paraffin Seetionin [D]. Chongqing: Southwest University, 2007: 19-34. [徐伟. 基于石蜡切片技术的家蚕胚胎发育与Mago蛋白定位研究 [D]. 重庆: 西南大学, 2007: 19-34]
Wu WG. A Collgction of Illustrative Photographs of the Histology & Embryology in the Silkworm (Bombyx mori)[M]. Beijing: China Agriculture Press, 1999: 77-95. [吴维光, 桑蚕组织胚胎学原理 [M]. 北京: 中国农业出版社, 1999: 77-95]
Landi S, Gargani E, Paoli F,et al. Morphological markers for cryopreservation in the embryonic development of Drosophila suzukii (Diptera: Drosophilidae)[J]. J. Econ. Entomol., 2015, 108: 1875-1883.
Lee TY. The development of Trichogramma evanescens Westw. and its influence on the embryonic development of its host, Attacus cynthia ricini Boisd[J]. Acta Entomologica Sinica, 1961, 10 (4-6): 339-354, 525-530. [利翠英. 赤眼蜂Trichogramma evanescens Westw.的个体发育及其对于寄主蓖麻蚕Attacus cynthia ricini Boisd.胚胎发育的影响 [J]. 昆虫学报, 1961, 10 (4-6): 339-354, 525-530]
Li JY, Moghaddam SH, Chen JE,et al. Shotgun proteomic analysis on the embryos of silkworm Bombyx mori at the end of organogenesis [J]. Insect Biochem. Mol. Biol., 2010, 40: 293-302.
Liu MJ, Tong XL, Zhang GJ. Advances in researches of the mechanism of diapause in the silkworm,Bombyx mori [J]. News letter of Sericultureal Science, 2014: 23-31.[刘玫静, 童晓玲, 张高军. 家蚕滞育机理研究进展 [J]. 蚕学通讯, 2014: 23-31]
Lu HS. Principles of the Silkworm Grainage [M]. Shanghai: Shanghai Scientific & Technical Publishers, 2011: 72-83, 95-103. [吕鸿声, 蚕种学原理[M]. 上海: 上海科学技术出版社, 2011: 72-83, 95-103]
Nagy L, Riddiford L, Kiguchi K. Morphogenesis in the early embryo of the lepidopteran Bombyx mori [J]. Dev. Biol., 1994, 165: 137-151.
Nakagaki M, Takei R, Nagashima E,et al. Cell cycles in embryos of the silkworm, Bombyx mori: G2-arrest at diapause stage [J]. Roux Arch. Dev. Biol., 1990, 200: 223-229.
Pan MH, Xu W, Su MK,et al. Study on the morphology of embryonic development states in silkworm, Bombyx mori [J]. Canye Kexue, 2007: 667-73. [潘敏慧, 徐伟, 苏茂科, 等. 家蚕胚胎发育过程的组织形态学研究 [J]. 蚕业科学, 2007: 667-73]
Ponnuvel KM, Murthy GN, Awasthi AK,et al. Differential gene expression during early embryonic development in diapause and non-diapause eggs of multivoltine silkworm Bombyx mori [J]. Indian J. Exp. Biol., 2010, 48: 1143-1151.
Sasibhushan S, Rao CGP, Ponnuvel KM. Genome wide microarray based expression profiles during early embryogenesis in diapause induced and non-diapause eggs of polyvoltine silkworm Bombyx mori [J]. Genomics, 2013, 102: 379-387.
Steponkus PL, Myers SP, Lynch DV,et al. Cryopreservation of Drosophila melanogaster embryos [J]. Nature, 1990, 345: 170-172.
Tu ZL, Hong DZ, Zhao LH. Division and movement observation and digitalization analysis of Bombyx mori fertilized nuclei in early embryonic development [J]. Science of Sericulture, 2014: 254-57. [屠振力, 洪德志, 赵丽华. 家蚕胚胎发育初期受精核的分裂与移动观察及数值化分析 [J]. 蚕业科学, 2014: 254-57]
Xiang ZH. Biology of Sericulture [M]. Beijing: China Forestry Publishing House, 2005: 70-89. [向仲怀. 蚕丝生物学 [M]. 北京: 中国林业出版社, 2005: 70-89]
Xu W. The Study of Embryonie Development and Immunoloealization of BmMago Based on Paraffin Seetionin [D]. Chongqing: Southwest University, 2007: 19-34. [徐伟. 基于石蜡切片技术的家蚕胚胎发育与Mago蛋白定位研究 [D]. 重庆: 西南大学, 2007: 19-34]
Wu WG. A Collgction of Illustrative Photographs of the Histology & Embryology in the Silkworm (Bombyx mori)[M]. Beijing: China Agriculture Press, 1999: 77-95. [吴维光, 桑蚕组织胚胎学原理 [M]. 北京: 中国农业出版社, 1999: 77-95]
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |