人工针阔混交林土壤微生物群落碳代谢特征
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摘要
利用BiologMT技术,研究了帽儿山试验林场中5种人工林(红松纯林、胡桃楸纯林、红松和胡桃楸混交林、水曲柳纯林、红松和水曲柳混交林)根际土壤微生物碳代谢特征,比较了混交林与相应纯林之间的差异。结果表明:胡桃楸纯林和水曲柳纯林(阔叶林)土壤微生物碳代谢活性(DAWC)高于红松纯林(针叶林);与红松混交后的胡桃楸林和水曲柳林土壤微生物碳代谢活性、物种多样性及均匀度均低于相应纯林。主成分分析表明:人工针阔叶纯林及混交林土壤微生物群落代谢的主要碳源类型是氨基酸、羧酸和糖类;胺类、氨基酸、羧酸、聚合物和糖类利用程度的显著降低,可能是造成混交后胡桃楸和水曲柳林土壤微生物碳代谢活性低于纯林的原因之一。氨基酸、羧酸和糖类利用程度的不同造成红松和胡桃楸混交林与相应纯林土壤微生物碳代谢功能差异。
The method of BiologMTwas taken to study the carbon metabolism characteristics of rhizospheric soil microbial from five types of plantation( Pinus koraiensis,Juglans mandshurica,Fraxinus mandshurica,Pinus koraiensis and Juglans mandshurica mixed plantation,Pinus koraiensis and Fraxinus mandshurica mixed plantation),and to compare differences on the functional diversity of carbon source metabolism of rhizospheric soil microbial community between pure plantation and mixed plantation. The average well color development( DAWC) of soil microbes of broad-leaved forest( Juglans mandshurica and Fraxinus mandshurica) was higher than that of coniferous forest( Pinus koraiensis). The carbon source metabolic activity of soil microbes and soil microbial community diversity in the broad-leaved forest( Juglans mandshurica and Fraxinus mandshurica) mixed with Pinus koraiensis were lower than those of relevant pure forests. By the principal component analysis( PCA),the main carbon source of soil microbial community metabolism in plantations included amino acids,carboxylic acid and carbohydrates,meanwhile which made the differences on soil carbon metabolic function between Pinus koraiensis and Juglans mandshurica mixed plantation and relevant pure plantation. The carbon metabolic activity of soil microbial community of Juglans mandshurica and Fraxinus mandshurica,which,respectively,mixed with Pinus koraiensis was obviously decreased due to less usage of amines,amino acids,carboxylic acid,polymers and carbohydrates.
The method of BiologMTwas taken to study the carbon metabolism characteristics of rhizospheric soil microbial from five types of plantation( Pinus koraiensis,Juglans mandshurica,Fraxinus mandshurica,Pinus koraiensis and Juglans mandshurica mixed plantation,Pinus koraiensis and Fraxinus mandshurica mixed plantation),and to compare differences on the functional diversity of carbon source metabolism of rhizospheric soil microbial community between pure plantation and mixed plantation. The average well color development( DAWC) of soil microbes of broad-leaved forest( Juglans mandshurica and Fraxinus mandshurica) was higher than that of coniferous forest( Pinus koraiensis). The carbon source metabolic activity of soil microbes and soil microbial community diversity in the broad-leaved forest( Juglans mandshurica and Fraxinus mandshurica) mixed with Pinus koraiensis were lower than those of relevant pure forests. By the principal component analysis( PCA),the main carbon source of soil microbial community metabolism in plantations included amino acids,carboxylic acid and carbohydrates,meanwhile which made the differences on soil carbon metabolic function between Pinus koraiensis and Juglans mandshurica mixed plantation and relevant pure plantation. The carbon metabolic activity of soil microbial community of Juglans mandshurica and Fraxinus mandshurica,which,respectively,mixed with Pinus koraiensis was obviously decreased due to less usage of amines,amino acids,carboxylic acid,polymers and carbohydrates.
引文
[1]张薇,魏海雷,高洪文,等.土壤微生物多样性及其环境影响因子研究进展[J].生态学杂志,2005,24(1):48-52.
[2] BALSER T,KINZIG A,FIRESTONE M. The functional consequences of biodiversity[J]. Austral Ecdogy,2002,28(6):684-689.
[3] CAVIGELLI M A,ROBERTSON G P. The functional significance of denitrified community composition in a terrestrial ecosystem[J].Ecology,2000,81(5):1402-1414.
[4] FIERER N,SCHIMEL J P,HOLDEN P A. Variations in microbial community composition through two soil depth profiles[J]. Soil Biology and Biochemistry,2003,35(1):167-176.
[5]陈新鹏.武夷山针阔混交林群落动态研究[D].福州:福建农林大学,2009.
[6]何友均,覃林,李智勇,等.西南桦纯林与西南桦×红椎混交林碳贮量比较[J].生态学报,2012,32(23):7586-7594.
[7]覃林,何友均,李智勇,等.南亚热带红椎马尾松纯林及其混交林生物量和生产力分配格局[J].林业科学,2011,47(12):17-21.
[8]衣晓丹,王新杰.杉木人工纯林与混交林下几种土壤养分对比及与生长的关系[J].中南林业科技大学学报,2013,33(2):34-38.
[9]刘福香.大兴安岭地区天然落叶松-白桦混交林分树种直径分布研究[D].哈尔滨:东北林业大学,2013.
[10]欧阳旭,李跃林,张倩媚.鼎湖山针阔叶混交林小气候调节效应[J].生态学杂志,2014,33(3):575-582.
[11]陈立新,姜一,段文标,等.红松混交林凋落物氮储量及分解释放对土壤氮的影响[J].生态学杂志,2015,34(1):114-121.
[12]张卫强,张卫华,潘文,等.桉树林和针阔混交林对植物多样性的影响比较[J].水土保持研究,2014,21(6):122-128.
[13] GARLAND J L,MILLS A L. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J]. Applied and Environmental Microbiology,1991,57(8):2351-2359.
[14] CLASSEN A T,BOYLE S I,HASKINS K E,et al. Communitylevel physiological profiles of bacteria and fungi:plate type and incubation temperature influences on contrasting soils[J]. FEMS Microbiology Ecology,2003,44(3):319-328.
[15] MAGURRAN A E. Ecological diversity and Its measurement[M].Princeton:Princeton University Press,1988.
[16] VERSTRTE W,VOETS J P. Soil microbial and biochemical characteristics in relation to soil management and fertility[J]. Soil Biology and Biochemistry,1977,9(4):253-258.
[17] BUYER J S,KAUFAMAN D D. Microbial diversity in the rhizosphere of corn grown under conventional and low-input systems[J]. Applied Soil Ecology,1997,5(1):21-27.
[18]张萌萌,敖红,张景云,等.建植年限对紫花苜蓿根际土壤微生物群落功能多样性的影响[J].草业科学,2014,31(5):787-796.
[19]罗希茜,郝晓晖,陈涛,等.长期不同施肥对稻田土壤微生物群落功能多样性的影响[J].生态学报,2009,29(2):740-748.
[20]林青,曾军,马晶,等.新疆地震断裂带次生植物根际土壤微生物碳源利用[J].应用生态学报,2011,22(9):2297-2302.
[21] GARLAND J L. Analysis and interpretation of community-level physiological profiles in microbial ecology[J]. FEMS Microbiology Ecology,1997,24(4):289-300.
[22] WAID J S. Does soil biodiversity depend upon metabiotic activity and influences[J]. Applied Soil Ecology,1999,13(2):151-158.
[23] CHODAK M,PIETRZYKOWSKI M,SROKA K. Physiological profiles of microbial communities in mine soils afforested with different tree species[J]. Ecological Engineering,2015,81(5):462-470.
[24]易志刚,蚁伟民,周丽霞,等.鼎湖山主要植被类型土壤微生物生物量研究[J].生态环境,2005,14(5):727-729.
[25] JIA S,WANG Z,LI X,et al. Effect of nitrogen fertilizer,root branch order and temperature on respiration and tissue N concentration of fine roots in Larix gmelinii and Fraxinus mandshurica[J]. Tree Physiology,2011,31(7):718-726.
[26] JIA S,MCLANGHLIN N B,GU J,et al. Relationships between root respiration rate and root morphology,chemistry and anatomy in Larix gmelinii and Fraxinus mandshurica[J]. Tree Physiology,2013,33(6):579-589.
[27]刘继明,黄炳军,徐演鹏,等.河南鸡公山自然保护区典型森林类型土壤微生物群落功能多样性[J].林业资源管理,2013(1):76-85.
[28] ZABINSKI C A,GANNON J E. Effects of recreational impacts on soil microbial communities[J]. Environmental Management,1997,21(2):233-238.
[29]陈法霖,郑华,阳柏苏,等.中亚热带几种针,阔叶树种凋落物混合分解对土壤微生物群落碳代谢多样性的影响[J].生态学报,2011,31(11):3027-3035.
[30]张俊艳.海南岛热带天然针叶林-阔叶林交错区的群落特征研究[D].北京:中国林业科学研究院,2014.
[31]孔维栋,刘可星,廖宗文.有机物料种类及腐熟水平对土壤微生物群落的影响[J].应用生态学报,2004,15(3):487-492.
[32]彭艳,刘艳霞,朱健,等.马尾松混交林土壤微生物功能多样性的影响[J].环境科学与技术,2014,37(11):42-46.
[33]谢英荷,洪坚平,卜玉山,等.枣麦间作对土壤肥力的影响[J].山西农业大学学报(自然科学版),2002,22(3):203-205.
[34] PRSTON M J,BODDY L,RANDERSON P F. Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles-a critique[J]. Fems Microbiology Ecology,2002,42(1):1-14.
[2] BALSER T,KINZIG A,FIRESTONE M. The functional consequences of biodiversity[J]. Austral Ecdogy,2002,28(6):684-689.
[3] CAVIGELLI M A,ROBERTSON G P. The functional significance of denitrified community composition in a terrestrial ecosystem[J].Ecology,2000,81(5):1402-1414.
[4] FIERER N,SCHIMEL J P,HOLDEN P A. Variations in microbial community composition through two soil depth profiles[J]. Soil Biology and Biochemistry,2003,35(1):167-176.
[5]陈新鹏.武夷山针阔混交林群落动态研究[D].福州:福建农林大学,2009.
[6]何友均,覃林,李智勇,等.西南桦纯林与西南桦×红椎混交林碳贮量比较[J].生态学报,2012,32(23):7586-7594.
[7]覃林,何友均,李智勇,等.南亚热带红椎马尾松纯林及其混交林生物量和生产力分配格局[J].林业科学,2011,47(12):17-21.
[8]衣晓丹,王新杰.杉木人工纯林与混交林下几种土壤养分对比及与生长的关系[J].中南林业科技大学学报,2013,33(2):34-38.
[9]刘福香.大兴安岭地区天然落叶松-白桦混交林分树种直径分布研究[D].哈尔滨:东北林业大学,2013.
[10]欧阳旭,李跃林,张倩媚.鼎湖山针阔叶混交林小气候调节效应[J].生态学杂志,2014,33(3):575-582.
[11]陈立新,姜一,段文标,等.红松混交林凋落物氮储量及分解释放对土壤氮的影响[J].生态学杂志,2015,34(1):114-121.
[12]张卫强,张卫华,潘文,等.桉树林和针阔混交林对植物多样性的影响比较[J].水土保持研究,2014,21(6):122-128.
[13] GARLAND J L,MILLS A L. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J]. Applied and Environmental Microbiology,1991,57(8):2351-2359.
[14] CLASSEN A T,BOYLE S I,HASKINS K E,et al. Communitylevel physiological profiles of bacteria and fungi:plate type and incubation temperature influences on contrasting soils[J]. FEMS Microbiology Ecology,2003,44(3):319-328.
[15] MAGURRAN A E. Ecological diversity and Its measurement[M].Princeton:Princeton University Press,1988.
[16] VERSTRTE W,VOETS J P. Soil microbial and biochemical characteristics in relation to soil management and fertility[J]. Soil Biology and Biochemistry,1977,9(4):253-258.
[17] BUYER J S,KAUFAMAN D D. Microbial diversity in the rhizosphere of corn grown under conventional and low-input systems[J]. Applied Soil Ecology,1997,5(1):21-27.
[18]张萌萌,敖红,张景云,等.建植年限对紫花苜蓿根际土壤微生物群落功能多样性的影响[J].草业科学,2014,31(5):787-796.
[19]罗希茜,郝晓晖,陈涛,等.长期不同施肥对稻田土壤微生物群落功能多样性的影响[J].生态学报,2009,29(2):740-748.
[20]林青,曾军,马晶,等.新疆地震断裂带次生植物根际土壤微生物碳源利用[J].应用生态学报,2011,22(9):2297-2302.
[21] GARLAND J L. Analysis and interpretation of community-level physiological profiles in microbial ecology[J]. FEMS Microbiology Ecology,1997,24(4):289-300.
[22] WAID J S. Does soil biodiversity depend upon metabiotic activity and influences[J]. Applied Soil Ecology,1999,13(2):151-158.
[23] CHODAK M,PIETRZYKOWSKI M,SROKA K. Physiological profiles of microbial communities in mine soils afforested with different tree species[J]. Ecological Engineering,2015,81(5):462-470.
[24]易志刚,蚁伟民,周丽霞,等.鼎湖山主要植被类型土壤微生物生物量研究[J].生态环境,2005,14(5):727-729.
[25] JIA S,WANG Z,LI X,et al. Effect of nitrogen fertilizer,root branch order and temperature on respiration and tissue N concentration of fine roots in Larix gmelinii and Fraxinus mandshurica[J]. Tree Physiology,2011,31(7):718-726.
[26] JIA S,MCLANGHLIN N B,GU J,et al. Relationships between root respiration rate and root morphology,chemistry and anatomy in Larix gmelinii and Fraxinus mandshurica[J]. Tree Physiology,2013,33(6):579-589.
[27]刘继明,黄炳军,徐演鹏,等.河南鸡公山自然保护区典型森林类型土壤微生物群落功能多样性[J].林业资源管理,2013(1):76-85.
[28] ZABINSKI C A,GANNON J E. Effects of recreational impacts on soil microbial communities[J]. Environmental Management,1997,21(2):233-238.
[29]陈法霖,郑华,阳柏苏,等.中亚热带几种针,阔叶树种凋落物混合分解对土壤微生物群落碳代谢多样性的影响[J].生态学报,2011,31(11):3027-3035.
[30]张俊艳.海南岛热带天然针叶林-阔叶林交错区的群落特征研究[D].北京:中国林业科学研究院,2014.
[31]孔维栋,刘可星,廖宗文.有机物料种类及腐熟水平对土壤微生物群落的影响[J].应用生态学报,2004,15(3):487-492.
[32]彭艳,刘艳霞,朱健,等.马尾松混交林土壤微生物功能多样性的影响[J].环境科学与技术,2014,37(11):42-46.
[33]谢英荷,洪坚平,卜玉山,等.枣麦间作对土壤肥力的影响[J].山西农业大学学报(自然科学版),2002,22(3):203-205.
[34] PRSTON M J,BODDY L,RANDERSON P F. Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles-a critique[J]. Fems Microbiology Ecology,2002,42(1):1-14.
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