鸡源H9N2亚型禽流感病毒感染鸡鸭和小鼠后对血液指标的影响
|
摘要
流感可以跨种属传播,通过了解地方性低致病禽流感H9N2亚型病毒感染不同种属动物后的血液变化情况,可为研究禽流感病毒的致病机制提供参考依据。选用52d黑羽乌骨鸡24只,52d樱桃谷鸭24只,6周龄～8周龄昆明系小鼠24只分成3组,攻毒前作为0d空白对照,鸡、鸭和小鼠采用静脉注射感染H9N2亚型流感病毒,分别在感染前(0d)、感染后3d、6d、9d采样,用PE-6800VET三分类动物血细胞分析仪测定血液常规指标。通过对不同种类白细胞和血小板数量变化的分析,结果表明,低致病性禽流感病毒H9N2可以跨种属感染,鸭炎性反应最小,小鼠炎性反应最严重。
Avian influenza virus can spread across species.After the local low pathogenic avian influenza subtype H9N2 virus infecting different species of animals,the changes of their blood can provide a reference for the pathogenic mechanism of avian influenza virus.Twenty four fifty-two days old dark feather silky chickens,twenty four fifty-two days old cherry valley ducks and twenty four six to eight weeks old Kunming mice were divided into three groups.Before injecting the virus as 0 day blank control,chickens,ducks and mice were infected with the H9N2 subtype influenza virus by intravenous injection.The blood was collected and hematologic indexes were measured by the PE-6800 VET animal blood cell analyzer 0 day before infection,3 days,6 days and 9 days after infection.The results showed that low pathogenic avian influenza virus H9N2 can spread across the species.Ducks inflammatory response was minimal and the inflammatory response of mice was most serious in the animals.
Avian influenza virus can spread across species.After the local low pathogenic avian influenza subtype H9N2 virus infecting different species of animals,the changes of their blood can provide a reference for the pathogenic mechanism of avian influenza virus.Twenty four fifty-two days old dark feather silky chickens,twenty four fifty-two days old cherry valley ducks and twenty four six to eight weeks old Kunming mice were divided into three groups.Before injecting the virus as 0 day blank control,chickens,ducks and mice were infected with the H9N2 subtype influenza virus by intravenous injection.The blood was collected and hematologic indexes were measured by the PE-6800 VET animal blood cell analyzer 0 day before infection,3 days,6 days and 9 days after infection.The results showed that low pathogenic avian influenza virus H9N2 can spread across the species.Ducks inflammatory response was minimal and the inflammatory response of mice was most serious in the animals.
引文
[1]Arafat N,Eladl A H,Marghani B H,et al.Enhanced infection of avian influenza virus H9N2with infectious laryngeotracheitis vaccination in chickens[J].Vet Microbiol,2018,219:8-16.
[2]王峥,柯昌文.全球禽流感流行概况及研究现状[J].华南预防医学,2017,43(2):182-186.
[3]Wu Y,Lin J,Yang S,et al.The molecular characteristics of avian influenza viruses(H9N2)derived from air samples in live poultry markets[J].Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis,2018,60:191-196.
[4]徐丹雯.H9N2亚型禽流感病毒的序列分析及其生物学特性研究[D].江苏扬州:扬州大学,2016.
[5]黄欣梅,李银,赵冬敏,等.11株H9N2亚型禽流感病毒神经氨酸酶基因遗传分析[J].南京农业大学学报,2016,39(1):150-155.
[6]孔兴天,杨孝朴,关立峥,等.两株H9N2亚型禽流感病毒的全基因组序列测定及其对小鼠的感染性研究[J].中国预防兽医学报,2017,39(10):779-783.
[7]Davison G M,Hendrickse H L,Matsha T E.The relationship between immunogenic red blood cell antigens and human Immunodeficiency virus infection[J].Transfusion Apheresis Sci,2018,57(1):58-62.
[8]Abdulcader M S,Ehiremen G,Nagy E,et al.Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+cells including macrophages yet decrease of the staining intensity of KUL01+cells using clodronate liposomes did not affect the viral genome loads in chickens.[J].Vet Immunol Immunopathol,2018,198:37-43.
[9]Safuan S N M,Tomari M R M,Wan N W Z.White blood cell(WBC)counting analysis in blood smear images using various color segmentation methods[J].Measurement,2018,116:543-555.
[10]张楚珺,欧德渊,姚红艳,等.组胺受体拮抗剂对低致病性禽流感患鸡血液指标的影响[J].畜牧与兽医,2017,49(2):98-102.
[11]赵维波,张钰,闵凡贵,等.禽流感H5N1亚型病毒对小鼠白细胞数值的影响[J].实验动物与比较医学,2006(2):108-111.
[12]王萍,李柱明,谢媛琪,等.诺如病毒对精神病患者感染性腹泻血液生理指标的影响[J].生物技术通讯,2016,27(2):254-256.
[13]桂博翔.SOCS3在H9N2禽流感病毒诱发急性肺损伤及继发细菌性肺炎中的作用研究[D].上海:华东师范大学,2016.
[14]Pushko P,Tretyakova I,Hidajat R,et al.Virus-like particles displaying H5,H7,H9hemagglutinins and N1neuraminidase elicit protective immunity to heterologous avian influenza viruses in chickens.[J].Virology,2017,501:176-182.
[15]Lion A,Richard M,Esnault E,et al.Productive replication of avian influenza viruses in chicken endothelial cells is determined by hemagglutinin cleavability and is related to innate immune escape[J].Virology,2018,513:29-42.
[16]誉铁鸥,曹泳文,张明翰,等.人感染H7N9禽流感重症患者免疫调理治疗前后淋巴细胞总数和T淋巴细胞亚群变化[J].岭南急诊医学杂志,2017,22(5):421-422+438.
[17]冯波.H9N2禽流感病毒感染内皮后对淋巴细胞抗病毒功能的影响[A]//中国畜牧兽医学会.第七届中国畜牧科技论坛论文集.北京:中国畜牧兽医学会,2016.
[18]刘永波,舒相华,吴常月,等.猪圆环病毒2型和猪伪狂犬病毒混合感染对仔猪血液生理生化指标的影响[J].中兽医学杂志,2017(2):3-5.
[2]王峥,柯昌文.全球禽流感流行概况及研究现状[J].华南预防医学,2017,43(2):182-186.
[3]Wu Y,Lin J,Yang S,et al.The molecular characteristics of avian influenza viruses(H9N2)derived from air samples in live poultry markets[J].Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis,2018,60:191-196.
[4]徐丹雯.H9N2亚型禽流感病毒的序列分析及其生物学特性研究[D].江苏扬州:扬州大学,2016.
[5]黄欣梅,李银,赵冬敏,等.11株H9N2亚型禽流感病毒神经氨酸酶基因遗传分析[J].南京农业大学学报,2016,39(1):150-155.
[6]孔兴天,杨孝朴,关立峥,等.两株H9N2亚型禽流感病毒的全基因组序列测定及其对小鼠的感染性研究[J].中国预防兽医学报,2017,39(10):779-783.
[7]Davison G M,Hendrickse H L,Matsha T E.The relationship between immunogenic red blood cell antigens and human Immunodeficiency virus infection[J].Transfusion Apheresis Sci,2018,57(1):58-62.
[8]Abdulcader M S,Ehiremen G,Nagy E,et al.Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+cells including macrophages yet decrease of the staining intensity of KUL01+cells using clodronate liposomes did not affect the viral genome loads in chickens.[J].Vet Immunol Immunopathol,2018,198:37-43.
[9]Safuan S N M,Tomari M R M,Wan N W Z.White blood cell(WBC)counting analysis in blood smear images using various color segmentation methods[J].Measurement,2018,116:543-555.
[10]张楚珺,欧德渊,姚红艳,等.组胺受体拮抗剂对低致病性禽流感患鸡血液指标的影响[J].畜牧与兽医,2017,49(2):98-102.
[11]赵维波,张钰,闵凡贵,等.禽流感H5N1亚型病毒对小鼠白细胞数值的影响[J].实验动物与比较医学,2006(2):108-111.
[12]王萍,李柱明,谢媛琪,等.诺如病毒对精神病患者感染性腹泻血液生理指标的影响[J].生物技术通讯,2016,27(2):254-256.
[13]桂博翔.SOCS3在H9N2禽流感病毒诱发急性肺损伤及继发细菌性肺炎中的作用研究[D].上海:华东师范大学,2016.
[14]Pushko P,Tretyakova I,Hidajat R,et al.Virus-like particles displaying H5,H7,H9hemagglutinins and N1neuraminidase elicit protective immunity to heterologous avian influenza viruses in chickens.[J].Virology,2017,501:176-182.
[15]Lion A,Richard M,Esnault E,et al.Productive replication of avian influenza viruses in chicken endothelial cells is determined by hemagglutinin cleavability and is related to innate immune escape[J].Virology,2018,513:29-42.
[16]誉铁鸥,曹泳文,张明翰,等.人感染H7N9禽流感重症患者免疫调理治疗前后淋巴细胞总数和T淋巴细胞亚群变化[J].岭南急诊医学杂志,2017,22(5):421-422+438.
[17]冯波.H9N2禽流感病毒感染内皮后对淋巴细胞抗病毒功能的影响[A]//中国畜牧兽医学会.第七届中国畜牧科技论坛论文集.北京:中国畜牧兽医学会,2016.
[18]刘永波,舒相华,吴常月,等.猪圆环病毒2型和猪伪狂犬病毒混合感染对仔猪血液生理生化指标的影响[J].中兽医学杂志,2017(2):3-5.