响应面法优化热水浸提灵芝孢子多糖工艺
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摘要
利用响应面设计试验优化灵芝孢子多糖的热水浸提工艺条件。以超临界CO_2萃取过灵芝孢子油后的灵芝孢子粉为原料,采用单因素试验,探究提取温度、提取时间、液料比对灵芝孢子多糖提取率的影响。在单因素试验的基础上,以多糖提取率为响应值,利用Box-Behnken试验设计和响应面分析法对提取条件进行优化,以确定最佳的提取工艺:提取温度91℃,提取时间3.3 h,液料比15.8︰1 (mL·g~(-1))。在此条件下多糖的提取率达到3.69%,与理论值相对误差0.33%。结果表明利用响应面法优化灵芝孢子多糖的热水浸提工艺是可行的。
Using response surface methodology to optimize hot water extraction conditions of polysaccharidies from Ganoderma Lucidum spores, based on Ganoderma lucidum spores which had been extracted Ganoderma Lucidum spores oil with supercritical carbon dioxide, using single factor experiment to investigated the effect of extraction temperature, extraction time and liquid-to-solid ratio on the yield of polysaccharides. Based on the single factor experiment, using the yield of polysaccharides as the response value, the extraction conditions by Box-Behnken design and response surface methodology to determine the optimal extraction conditions are optimized. The result show that the optimal extraction conditions are extraction temperature 91 ℃, extraction time 3.3 h and the ratio of liquid-to-solid 15.8︰1(mL·g~(-1)). The yield of polysaccharides is 3.69%under this conditions, compared with the theoretical value, the relative error is 0.33%. The results indicate that it is reliable,using response surface methodology to optimize hot water extraction conditions of polysaccharidies from Ganoderma lucidum spores.
Using response surface methodology to optimize hot water extraction conditions of polysaccharidies from Ganoderma Lucidum spores, based on Ganoderma lucidum spores which had been extracted Ganoderma Lucidum spores oil with supercritical carbon dioxide, using single factor experiment to investigated the effect of extraction temperature, extraction time and liquid-to-solid ratio on the yield of polysaccharides. Based on the single factor experiment, using the yield of polysaccharides as the response value, the extraction conditions by Box-Behnken design and response surface methodology to determine the optimal extraction conditions are optimized. The result show that the optimal extraction conditions are extraction temperature 91 ℃, extraction time 3.3 h and the ratio of liquid-to-solid 15.8︰1(mL·g~(-1)). The yield of polysaccharides is 3.69%under this conditions, compared with the theoretical value, the relative error is 0.33%. The results indicate that it is reliable,using response surface methodology to optimize hot water extraction conditions of polysaccharidies from Ganoderma lucidum spores.
引文
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[20]于海洋,弥春霞,吴薇,等.灵芝多糖提取方式比较及多糖应用研究进展[J].安徽农业科学, 2014, 42(8):2318-2319.
[21]辛敏,刘轩,詹欣,等.硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J].中国实验方剂学杂志, 2014,20(3):62-65.
[22]毛淑敏,许家珍,周帅飞,等.苯酚-硫酸法联合DNS法测定金银花不同花期多糖的含量[J].中南药学, 2015, 13(1):65-67.
[23]李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索, 2015, 34(8):41-45.
[24]董万领,闫晓明,程江华,等.响应面法优化水提菜籽多糖工艺[J].中国油脂, 2014, 39(4):87-89.
[25]李颖畅,王亚丽,齐凤元,等.响应面法优化蓝莓叶多糖提取工艺[J].食品工业科技, 2015, 36(3):227-231.
[2]王卫霄,吕艳茹,姚苗苗,等.灵芝孢子粉抗肿瘤活性的研究进展[J].河北医药, 2015, 37(1):105-108.
[3]王健,焦强,王海波,等.灵芝孢子粉质量分析方法研究进展[J].药物分析杂志, 2016, 36(5):749-755.
[4] WANG P Y, ZHU X L, LIN Z B. Antitumor and immunomodulatory effects of polysaccharides from broken-spore of Ganoderma lucidum[J]. Frontiers in Pharmacology, 2012(3):135.
[5] GUO L, XIE J, RUAN Y, et al. Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum[J]. International Immunopharmacology,2009, 9(10):1175-1182.
[6] MA C, GUAN S, YANG M, et al. Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum[J].Phytomedicine International Journal of Phytotherapy&Phytopharmacology, 2008, 15(4):268-276.
[7] BAO X, FANG J, LI X Y. Structural characterization and immunomodulating activity of a complex glucan from spores of Ganoderma lucidum[J]. Bioscience Biotechnology&Biochemistry, 2001, 65(11):2384-2391.
[8] ZHU X, CHEN X, XIE J, et al. Mechanochemical-assisted extraction and antioxidant activity of polysaccharides from Ganoderma lucidum spores[J]. International Journal of Food Science&Technology, 2012, 47(5):927-932.
[9] ZHOU Y, QU Z Q, ZENG Y S, et al. Neuroprotective effect of preadministration with Ganoderma lucidum spore on rat hippocampus[J]. Experimental&Toxicologic Pathology,2012, 64(7/8):673-680.
[10]常珊珊,周丹,孟国梁,等.灵芝多糖对高脂性脂肪肝大鼠氧化应激的影响[J].中国中药杂志, 2012, 37(20):9-13.
[11]朱科学,聂少平,宋丹,等.黑灵芝多糖对Ⅱ型糖尿病大鼠血糖、血脂及肠系膜上动脉病变改善作用[J].食品科学, 2013, 34(23):300-304.
[12]叶鹏飞,张美萍,王康宇,等.灵芝主要成分及其药理作用的研究进展综述[J].食药用菌, 2013, 21(3):158-161.
[13]朱玲,史吉平,王晨光,等.灵芝多糖的提取方法及其功能特性研究进展[J].现代化工, 2017, 37(1):55-59.
[14]于华峥,刘艳芳,周帅,等.灵芝子实体、菌丝体及孢子粉中多糖成分差异比较研究[J].菌物学报, 2016, 35(2):170-177.
[15]尚姝,于青. HPLC-ELSD法分析破壁灵芝孢子粉的多糖组成[J].中成药, 2015, 37(6):1272-1275.
[16]刘佳,王勇.灵芝多糖的研究进展[J].现代药物与临床,2012, 27(6):629-634.
[17]游丽君,冯梦莹,刘钧发,等.不同提取方法对灵芝多糖性质的影响研究[J].现代食品科技, 2013, 29(6):1207-1212.
[18]邓辰辰,相继芬,董兴叶,等.亚临界水提取灵芝多糖的工艺研究[J].河南工业大学学报(自然科学版), 2016,37(2):105-108.
[19]郑丹婷,蔡思敏,苑子涵,等.灵芝多糖的提取、分离及分析方法的研究进展[J].机电信息, 2016(11):33-38.
[20]于海洋,弥春霞,吴薇,等.灵芝多糖提取方式比较及多糖应用研究进展[J].安徽农业科学, 2014, 42(8):2318-2319.
[21]辛敏,刘轩,詹欣,等.硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J].中国实验方剂学杂志, 2014,20(3):62-65.
[22]毛淑敏,许家珍,周帅飞,等.苯酚-硫酸法联合DNS法测定金银花不同花期多糖的含量[J].中南药学, 2015, 13(1):65-67.
[23]李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索, 2015, 34(8):41-45.
[24]董万领,闫晓明,程江华,等.响应面法优化水提菜籽多糖工艺[J].中国油脂, 2014, 39(4):87-89.
[25]李颖畅,王亚丽,齐凤元,等.响应面法优化蓝莓叶多糖提取工艺[J].食品工业科技, 2015, 36(3):227-231.